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P28622

- GUN4_BACS5

UniProt

P28622 - GUN4_BACS5

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Protein
Endoglucanase 4
Gene
N/A
Organism
Bacillus sp. (strain KSM-522)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei400 – 4001 By similarity
Active sitei438 – 4381 By similarity
Active sitei447 – 4471 By similarity

GO - Molecular functioni

  1. cellulase activity Source: UniProtKB-EC
  2. cellulose binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiCBM3. Carbohydrate-Binding Module Family 3.
GH9. Glycoside Hydrolase Family 9.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase 4 (EC:3.2.1.4)
Alternative name(s):
Cellulase 4
EG-IV
Endo-1,4-beta-glucanase 4
OrganismiBacillus sp. (strain KSM-522)
Taxonomic identifieri120046 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525 Reviewed prediction
Add
BLAST
Chaini26 – 636611Endoglucanase 4
PRO_0000007952Add
BLAST

Proteomic databases

PRIDEiP28622.

Structurei

3D structure databases

ProteinModelPortaliP28622.
SMRiP28622. Positions 27-634.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini478 – 635158CBM3
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
2.60.40.710. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR008965. Carb-bd_dom.
IPR001956. CBD_3.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view]
PfamiPF00942. CBM_3. 1 hit.
PF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SMARTiSM01067. CBM_3. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
SSF49384. SSF49384. 1 hit.
PROSITEiPS51172. CBM3. 1 hit.
PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28622-1 [UniParc]FASTAAdd to Basket

« Hide

MTRRWSFLVQ CFTFKKKEGV RSRYMSDYNY VEVLQKSILF YEAQRSGKLP    50
ESNRLNWRGD SGLEDGKDVG HDLTGGWYDA GDHVKFGLPM AYSAAVLAWT 100
VYEYREAYEE AELLDDMLDQ IKWATDYFLK AHTGPNEFWA QVGDGNADHG 150
WWGPAEVMPM NRPAFKIDEH CPGTEVAAQT AAALAAGSII FKETDAPYAA 200
KLLTHAKQLY AFADQYRGEY TDCVTNAQPF YNSWSGYIDE LIWGGIWLYL 250
ATNDQTYLNK ALKAVEEWPK DWDYTFTMSW DNTFFLSQIL LARITKEKRF 300
IESTERNLDY WSTGFVQNGK VERITYTPGG LAWLDQWGSL RYTANAAFLA 350
FVYADWVSDQ EKKNRYQTFA IRQTHYMLGD NPQNRSYVVG FGKNPPMHPH 400
HRTAHGSWSN QLTTPSSHRH TLYGPLVGGP NRQDQYTDDI SDYVSNEVAT 450
DYNAAFTGNG AAVWSGQSKL PNFPPKEKVE DEFFVEAAVM SNDTTSTQIK 500
AILYNRSGWP ARSSQSLSFR YYVNLSEIFA KGFTDKDIQV TAVYNEGASL 550
SPLTVYDASS HIYFTEIDFT GVAIFPGGES LHKKEIQFRL SAPNGANIWD 600
ASNDYSFQGL TSNMQKTARI PVFDQGDLVF GTLPNK 636
Length:636
Mass (Da):72,245
Last modified:May 30, 2000 - v2
Checksum:i76718BF57A8EF6A2
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti530 – 5345AKGFT → CEGIH1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB004098 Genomic DNA. Translation: BAA24918.1.
PIRiJC5874.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB004098 Genomic DNA. Translation: BAA24918.1 .
PIRi JC5874.

3D structure databases

ProteinModelPortali P28622.
SMRi P28622. Positions 27-634.
ModBasei Search...

Protein family/group databases

CAZyi CBM3. Carbohydrate-Binding Module Family 3.
GH9. Glycoside Hydrolase Family 9.

Proteomic databases

PRIDEi P28622.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.50.10.10. 1 hit.
2.60.40.710. 1 hit.
InterProi IPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR008965. Carb-bd_dom.
IPR001956. CBD_3.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view ]
Pfami PF00942. CBM_3. 1 hit.
PF00759. Glyco_hydro_9. 1 hit.
[Graphical view ]
SMARTi SM01067. CBM_3. 1 hit.
[Graphical view ]
SUPFAMi SSF48208. SSF48208. 1 hit.
SSF49384. SSF49384. 1 hit.
PROSITEi PS51172. CBM3. 1 hit.
PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Amino acid sequence and stereoselective hydrolytic reaction of an endo-1,4-beta-glucanase from a Bacillus strain."
    Hitomi J., Hatada Y., Kawaminami S., Kawai S., Ito S.
    Biosci. Biotechnol. Biochem. 61:2004-2009(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Cloning of Clostridium cellulovorans endo-1,4-beta-glucanase genes."
    Shoseyov O., Foong F., Hamamoto T., Doi R.H.
    Biochem. Biophys. Res. Commun. 169:667-672(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-534.
  3. Doi R.H.
    Unpublished observations (JAN-2000)
    Cited for: SHOWS THAT SEQUENCE DID NOT ORIGINATE FROM C.CELLULOVORANS.

Entry informationi

Entry nameiGUN4_BACS5
AccessioniPrimary (citable) accession number: P28622
Secondary accession number(s): O50589
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: May 30, 2000
Last modified: September 3, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally (1 Publication) thought to originate from Clostridium cellulovorans and was termed endoglucanase C (engC).

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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