P28621 (GUNB_CLOC7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase B
EC=3.2.1.4

Alternative name(s):
Cellulase B
Endo-1,4-beta-glucanase B

Gene names

Name:	engB
Ordered Locus Names:	Clocel_1150

Organism

Clostridium cellulovorans (strain ATCC 35296 / DSM 3052 / OCM 3 / 743B) [Complete proteome] [HAMAP]

Taxonomic identifier

573061 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium › Clostridium cellulovorans

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Has endoglucanase activity on carboxymethyl-cellulose (CMC), xylan and lichenan, but not Avicel.
Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans. Endohydrolysis of 1,4-beta-D-xylosidic linkages in xylans.
Domain	A 24 residue domain is repeated twice in this enzyme as well as in other C.thermocellum cellulosome enzymes. This domain may function as the binding ligand for the SL component.
Miscellaneous	Up to 32 AA of the N-terminus and 52 AA of the C-terminus are not required for catalytic activity.
Sequence similarities	Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 33	33	Potential
	Chain	34 – 440	407	Endoglucanase B		PRO_0000007847
Regions
	Repeat	387 – 410	24	1
	Repeat	419 – 440	22	2
	Region	387 – 440	54	2 X 24 AA approximate repeats
Sites
	Active site	179	1	Proton donor By similarity
	Active site	305	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	P28621 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 93D6CB9EA7E2AF21	FASTA	440	48,637
	10 20 30 40 50 60 MNKRLSRGKI SLLASVFVTT TFMGGVNVLA STAKTGIRDI TSQQVVKEMK VGWNLGNTMD 70 80 90 100 110 120 ATGGETNWGN PLTTHAMIDK VKAAGFNTLR LPITWDGHIG AAPDYAIDAT WMNRVEEIAN 130 140 150 160 170 180 YAFDNNMYVI INLHHEDGWL KPYYANEAEV KAKITKVWTQ IANRFKDYGD YLIFETMNEP 190 200 210 220 230 240 RPVGAADEWS GGSYENRDMV NRYNLTAVNT IRATGGNNAL RHIMVPTLAA AALSTTMNDY 250 260 270 280 290 300 IVPNNDSRVI VSLHMYSPYF FSADLTSQWT TATWGSDADK AALSADFDAV YNKFVKNGRA 310 320 330 340 350 360 VVIGEMGTIN KNNLDSRVKH AEYYAKEATV RGITPIWWDN GYCVAGKEQT FGIFNRKNLT 370 380 390 400 410 420 WCCPEVMQAF IRGAGATQTQ TSYSLGDVNK DGKVNAIDYA VLKSILLGTN TNVDLSVSDM 430 440 NKDGKVNALD LAVLKKMLLS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence and characteristics of endoglucanase gene engB from Clostridium cellulovorans." Foong F., Hamamoto T., Shoseyov O., Doi R.H. J. Gen. Microbiol. 137:1729-1736(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequence of Clostridium cellulovorans 743B." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L., Pitluck S., Chertkov O., Detter J.C., Han C., Tapia R., Land M., Hauser L., Chang Y.-J., Jeffries C., Kyrpides N., Ivanova N., Mikhailova N., Hemme C.L., Woyke T. Submitted (AUG-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35296 / DSM 3052 / OCM 3 / 743B.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M75706 Genomic DNA. Translation: AAA23231.1. CP002160 Genomic DNA. Translation: ADL50906.1.
PIR	A44815.
RefSeq	YP_003842670.1. NC_014393.1.
3D structure databases
ProteinModelPortal	P28621.
ModBase	Search...
Protein-protein interaction databases
IntAct	P28621. 1 interaction.
Protein family/group databases
CAZy	GH5. Glycoside Hydrolase Family 5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ADL50906; ADL50906; Clocel_1150.
GeneID	9607996.
KEGG	ccb:Clocel_1150.
PATRIC	41738611. VBICloCel81632203721_3056.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000027405.
KO	K01179.
ProtClustDB	CLSK882585.
Enzyme and pathway databases
BioCyc	CCEL573061:GIXD-1188-MONOMER.
Family and domain databases
Gene3D	1.10.1330.10. 1 hit. 3.20.20.80. 1 hit.
InterPro	IPR016134. Cellulos_enz_dockerin_1. IPR002105. Cellulos_enz_dockerin_1_Ca-bd. IPR018242. Dockerin_1. IPR001547. Glyco_hydro_5. IPR018087. Glyco_hydro_5_CS. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00150. Cellulase. 1 hit. PF00404. Dockerin_1. 2 hits. [Graphical view]
SUPFAM	SSF63446. Cellulos_enz_dockerin_1. 1 hit. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00448. CLOS_CELLULOSOME_RPT. 2 hits. PS00018. EF_HAND_1. 2 hits. Uncertain. PS00659. GLYCOSYL_HYDROL_F5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUNB_CLOC7

Accession

Primary (citable) accession number: P28621
Secondary accession number(s): D9SUK1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program