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P28621

- GUNB_CLOC7

UniProt

P28621 - GUNB_CLOC7

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Protein

Endoglucanase B

Gene

engB

Organism
Clostridium cellulovorans (strain ATCC 35296 / DSM 3052 / OCM 3 / 743B)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Has endoglucanase activity on carboxymethyl-cellulose (CMC), xylan and lichenan, but not Avicel.

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Endohydrolysis of 1,4-beta-D-xylosidic linkages in xylans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei179 – 1791Proton donorBy similarity
Active sitei305 – 3051NucleophileBy similarity

GO - Molecular functioni

  1. cellulase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciCCEL573061:GIXD-1188-MONOMER.

Protein family/group databases

CAZyiGH5. Glycoside Hydrolase Family 5.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase B (EC:3.2.1.4)
Alternative name(s):
Cellulase B
Endo-1,4-beta-glucanase B
Gene namesi
Name:engB
Ordered Locus Names:Clocel_1150
OrganismiClostridium cellulovorans (strain ATCC 35296 / DSM 3052 / OCM 3 / 743B)
Taxonomic identifieri573061 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000002730: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3333Sequence AnalysisAdd
BLAST
Chaini34 – 440407Endoglucanase BPRO_0000007847Add
BLAST

Interactioni

Protein-protein interaction databases

IntActiP28621. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP28621.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati387 – 410241Add
BLAST
Repeati419 – 440222Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni387 – 440542 X 24 AA approximate repeatsAdd
BLAST

Domaini

A 24 residue domain is repeated twice in this enzyme as well as in other C.thermocellum cellulosome enzymes. This domain may function as the binding ligand for the SL component.

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

HOGENOMiHOG000027405.
KOiK01179.
OMAiRFKDYGD.

Family and domain databases

Gene3Di1.10.1330.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR016134. Cellulos_enz_dockerin_1.
IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
IPR018242. Dockerin_1.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00150. Cellulase. 1 hit.
PF00404. Dockerin_1. 2 hits.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF63446. SSF63446. 1 hit.
PROSITEiPS00448. CLOS_CELLULOSOME_RPT. 2 hits.
PS00018. EF_HAND_1. 2 hits.
PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28621-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNKRLSRGKI SLLASVFVTT TFMGGVNVLA STAKTGIRDI TSQQVVKEMK
60 70 80 90 100
VGWNLGNTMD ATGGETNWGN PLTTHAMIDK VKAAGFNTLR LPITWDGHIG
110 120 130 140 150
AAPDYAIDAT WMNRVEEIAN YAFDNNMYVI INLHHEDGWL KPYYANEAEV
160 170 180 190 200
KAKITKVWTQ IANRFKDYGD YLIFETMNEP RPVGAADEWS GGSYENRDMV
210 220 230 240 250
NRYNLTAVNT IRATGGNNAL RHIMVPTLAA AALSTTMNDY IVPNNDSRVI
260 270 280 290 300
VSLHMYSPYF FSADLTSQWT TATWGSDADK AALSADFDAV YNKFVKNGRA
310 320 330 340 350
VVIGEMGTIN KNNLDSRVKH AEYYAKEATV RGITPIWWDN GYCVAGKEQT
360 370 380 390 400
FGIFNRKNLT WCCPEVMQAF IRGAGATQTQ TSYSLGDVNK DGKVNAIDYA
410 420 430 440
VLKSILLGTN TNVDLSVSDM NKDGKVNALD LAVLKKMLLS
Length:440
Mass (Da):48,637
Last modified:December 1, 1992 - v1
Checksum:i93D6CB9EA7E2AF21
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M75706 Genomic DNA. Translation: AAA23231.1.
CP002160 Genomic DNA. Translation: ADL50906.1.
PIRiA44815.
RefSeqiWP_010076241.1. NZ_BABR01000336.1.
YP_003842670.1. NC_014393.1.

Genome annotation databases

EnsemblBacteriaiADL50906; ADL50906; Clocel_1150.
GeneIDi9607996.
KEGGiccb:Clocel_1150.
PATRICi41738611. VBICloCel81632203721_3056.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M75706 Genomic DNA. Translation: AAA23231.1 .
CP002160 Genomic DNA. Translation: ADL50906.1 .
PIRi A44815.
RefSeqi WP_010076241.1. NZ_BABR01000336.1.
YP_003842670.1. NC_014393.1.

3D structure databases

ProteinModelPortali P28621.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P28621. 1 interaction.

Protein family/group databases

CAZyi GH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADL50906 ; ADL50906 ; Clocel_1150 .
GeneIDi 9607996.
KEGGi ccb:Clocel_1150.
PATRICi 41738611. VBICloCel81632203721_3056.

Phylogenomic databases

HOGENOMi HOG000027405.
KOi K01179.
OMAi RFKDYGD.

Enzyme and pathway databases

BioCyci CCEL573061:GIXD-1188-MONOMER.

Family and domain databases

Gene3Di 1.10.1330.10. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR016134. Cellulos_enz_dockerin_1.
IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
IPR018242. Dockerin_1.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF00150. Cellulase. 1 hit.
PF00404. Dockerin_1. 2 hits.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF63446. SSF63446. 1 hit.
PROSITEi PS00448. CLOS_CELLULOSOME_RPT. 2 hits.
PS00018. EF_HAND_1. 2 hits.
PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence and characteristics of endoglucanase gene engB from Clostridium cellulovorans."
    Foong F., Hamamoto T., Shoseyov O., Doi R.H.
    J. Gen. Microbiol. 137:1729-1736(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35296 / DSM 3052 / OCM 3 / 743B.

Entry informationi

Entry nameiGUNB_CLOC7
AccessioniPrimary (citable) accession number: P28621
Secondary accession number(s): D9SUK1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: October 1, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Up to 32 AA of the N-terminus and 52 AA of the C-terminus are not required for catalytic activity.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3