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Protein

Endoglucanase B

Gene

engB

Organism
Clostridium cellulovorans (strain ATCC 35296 / DSM 3052 / OCM 3 / 743B)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Has endoglucanase activity on carboxymethyl-cellulose (CMC), xylan and lichenan, but not Avicel.

Miscellaneous

Up to 32 AA of the N-terminus and 52 AA of the C-terminus are not required for catalytic activity.

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei179Proton donorBy similarity1
Active sitei305NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciCCEL573061:G1GMK-1162-MONOMER

Protein family/group databases

CAZyiGH5 Glycoside Hydrolase Family 5

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase B (EC:3.2.1.4)
Alternative name(s):
Cellulase B
Endo-1,4-beta-glucanase B
Endo-1,4-beta-xylanase (EC:3.2.1.8)
Gene namesi
Name:engB
Ordered Locus Names:Clocel_1150
OrganismiClostridium cellulovorans (strain ATCC 35296 / DSM 3052 / OCM 3 / 743B)
Taxonomic identifieri573061 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000002730 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 33Sequence analysisAdd BLAST33
ChainiPRO_000000784734 – 440Endoglucanase BAdd BLAST407

Proteomic databases

PRIDEiP28621

Interactioni

Protein-protein interaction databases

IntActiP28621, 1 interactor
STRINGi573061.Clocel_1150

Structurei

3D structure databases

ProteinModelPortaliP28621
SMRiP28621
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini381 – 440DockerinPROSITE-ProRule annotationAdd BLAST60

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108K2F Bacteria
COG2730 LUCA
HOGENOMiHOG000027405
KOiK01179
OMAiWDQYANQ
OrthoDBiPOG091H14IK

Family and domain databases

InterProiView protein in InterPro
IPR002105 Dockerin_1_rpt
IPR016134 Dockerin_dom
IPR036439 Dockerin_dom_sf
IPR001547 Glyco_hydro_5
IPR018087 Glyco_hydro_5_CS
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00150 Cellulase, 1 hit
PF00404 Dockerin_1, 2 hits
SUPFAMiSSF51445 SSF51445, 1 hit
SSF63446 SSF63446, 1 hit
PROSITEiView protein in PROSITE
PS00448 CLOS_CELLULOSOME_RPT, 2 hits
PS51766 DOCKERIN, 1 hit
PS00018 EF_HAND_1, 2 hits
PS00659 GLYCOSYL_HYDROL_F5, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28621-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKRLSRGKI SLLASVFVTT TFMGGVNVLA STAKTGIRDI TSQQVVKEMK
60 70 80 90 100
VGWNLGNTMD ATGGETNWGN PLTTHAMIDK VKAAGFNTLR LPITWDGHIG
110 120 130 140 150
AAPDYAIDAT WMNRVEEIAN YAFDNNMYVI INLHHEDGWL KPYYANEAEV
160 170 180 190 200
KAKITKVWTQ IANRFKDYGD YLIFETMNEP RPVGAADEWS GGSYENRDMV
210 220 230 240 250
NRYNLTAVNT IRATGGNNAL RHIMVPTLAA AALSTTMNDY IVPNNDSRVI
260 270 280 290 300
VSLHMYSPYF FSADLTSQWT TATWGSDADK AALSADFDAV YNKFVKNGRA
310 320 330 340 350
VVIGEMGTIN KNNLDSRVKH AEYYAKEATV RGITPIWWDN GYCVAGKEQT
360 370 380 390 400
FGIFNRKNLT WCCPEVMQAF IRGAGATQTQ TSYSLGDVNK DGKVNAIDYA
410 420 430 440
VLKSILLGTN TNVDLSVSDM NKDGKVNALD LAVLKKMLLS
Length:440
Mass (Da):48,637
Last modified:December 1, 1992 - v1
Checksum:i93D6CB9EA7E2AF21
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M75706 Genomic DNA Translation: AAA23231.1
CP002160 Genomic DNA Translation: ADL50906.1
PIRiA44815
RefSeqiWP_010076241.1, NC_014393.1

Genome annotation databases

EnsemblBacteriaiADL50906; ADL50906; Clocel_1150
KEGGiccb:Clocel_1150

Similar proteinsi

Entry informationi

Entry nameiGUNB_CLOC7
AccessioniPrimary (citable) accession number: P28621
Secondary accession number(s): D9SUK1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: May 23, 2018
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
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