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P28621

- GUNB_CLOC7

UniProt

P28621 - GUNB_CLOC7

Protein

Endoglucanase B

Gene

engB

Organism
Clostridium cellulovorans (strain ATCC 35296 / DSM 3052 / OCM 3 / 743B)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Dec 1992)
      Previous versions | rss
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    Functioni

    Has endoglucanase activity on carboxymethyl-cellulose (CMC), xylan and lichenan, but not Avicel.

    Catalytic activityi

    Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
    Endohydrolysis of 1,4-beta-D-xylosidic linkages in xylans.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei179 – 1791Proton donorBy similarity
    Active sitei305 – 3051NucleophileBy similarity

    GO - Molecular functioni

    1. cellulase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellulose catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

    Enzyme and pathway databases

    BioCyciCCEL573061:GIXD-1188-MONOMER.

    Protein family/group databases

    CAZyiGH5. Glycoside Hydrolase Family 5.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoglucanase B (EC:3.2.1.4)
    Alternative name(s):
    Cellulase B
    Endo-1,4-beta-glucanase B
    Gene namesi
    Name:engB
    Ordered Locus Names:Clocel_1150
    OrganismiClostridium cellulovorans (strain ATCC 35296 / DSM 3052 / OCM 3 / 743B)
    Taxonomic identifieri573061 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
    ProteomesiUP000002730: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3333Sequence AnalysisAdd
    BLAST
    Chaini34 – 440407Endoglucanase BPRO_0000007847Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    IntActiP28621. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP28621.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati387 – 410241Add
    BLAST
    Repeati419 – 440222Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni387 – 440542 X 24 AA approximate repeatsAdd
    BLAST

    Domaini

    A 24 residue domain is repeated twice in this enzyme as well as in other C.thermocellum cellulosome enzymes. This domain may function as the binding ligand for the SL component.

    Sequence similaritiesi

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    HOGENOMiHOG000027405.
    KOiK01179.
    OMAiRFKDYGD.

    Family and domain databases

    Gene3Di1.10.1330.10. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR016134. Cellulos_enz_dockerin_1.
    IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
    IPR018242. Dockerin_1.
    IPR001547. Glyco_hydro_5.
    IPR018087. Glyco_hydro_5_CS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF00150. Cellulase. 1 hit.
    PF00404. Dockerin_1. 2 hits.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF63446. SSF63446. 1 hit.
    PROSITEiPS00448. CLOS_CELLULOSOME_RPT. 2 hits.
    PS00018. EF_HAND_1. 2 hits.
    PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P28621-1 [UniParc]FASTAAdd to Basket

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    MNKRLSRGKI SLLASVFVTT TFMGGVNVLA STAKTGIRDI TSQQVVKEMK    50
    VGWNLGNTMD ATGGETNWGN PLTTHAMIDK VKAAGFNTLR LPITWDGHIG 100
    AAPDYAIDAT WMNRVEEIAN YAFDNNMYVI INLHHEDGWL KPYYANEAEV 150
    KAKITKVWTQ IANRFKDYGD YLIFETMNEP RPVGAADEWS GGSYENRDMV 200
    NRYNLTAVNT IRATGGNNAL RHIMVPTLAA AALSTTMNDY IVPNNDSRVI 250
    VSLHMYSPYF FSADLTSQWT TATWGSDADK AALSADFDAV YNKFVKNGRA 300
    VVIGEMGTIN KNNLDSRVKH AEYYAKEATV RGITPIWWDN GYCVAGKEQT 350
    FGIFNRKNLT WCCPEVMQAF IRGAGATQTQ TSYSLGDVNK DGKVNAIDYA 400
    VLKSILLGTN TNVDLSVSDM NKDGKVNALD LAVLKKMLLS 440
    Length:440
    Mass (Da):48,637
    Last modified:December 1, 1992 - v1
    Checksum:i93D6CB9EA7E2AF21
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M75706 Genomic DNA. Translation: AAA23231.1.
    CP002160 Genomic DNA. Translation: ADL50906.1.
    PIRiA44815.
    RefSeqiWP_010076241.1. NZ_BABR01000336.1.
    YP_003842670.1. NC_014393.1.

    Genome annotation databases

    EnsemblBacteriaiADL50906; ADL50906; Clocel_1150.
    GeneIDi9607996.
    KEGGiccb:Clocel_1150.
    PATRICi41738611. VBICloCel81632203721_3056.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M75706 Genomic DNA. Translation: AAA23231.1 .
    CP002160 Genomic DNA. Translation: ADL50906.1 .
    PIRi A44815.
    RefSeqi WP_010076241.1. NZ_BABR01000336.1.
    YP_003842670.1. NC_014393.1.

    3D structure databases

    ProteinModelPortali P28621.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P28621. 1 interaction.

    Protein family/group databases

    CAZyi GH5. Glycoside Hydrolase Family 5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADL50906 ; ADL50906 ; Clocel_1150 .
    GeneIDi 9607996.
    KEGGi ccb:Clocel_1150.
    PATRICi 41738611. VBICloCel81632203721_3056.

    Phylogenomic databases

    HOGENOMi HOG000027405.
    KOi K01179.
    OMAi RFKDYGD.

    Enzyme and pathway databases

    BioCyci CCEL573061:GIXD-1188-MONOMER.

    Family and domain databases

    Gene3Di 1.10.1330.10. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR016134. Cellulos_enz_dockerin_1.
    IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
    IPR018242. Dockerin_1.
    IPR001547. Glyco_hydro_5.
    IPR018087. Glyco_hydro_5_CS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF00150. Cellulase. 1 hit.
    PF00404. Dockerin_1. 2 hits.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF63446. SSF63446. 1 hit.
    PROSITEi PS00448. CLOS_CELLULOSOME_RPT. 2 hits.
    PS00018. EF_HAND_1. 2 hits.
    PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence and characteristics of endoglucanase gene engB from Clostridium cellulovorans."
      Foong F., Hamamoto T., Shoseyov O., Doi R.H.
      J. Gen. Microbiol. 137:1729-1736(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 35296 / DSM 3052 / OCM 3 / 743B.

    Entry informationi

    Entry nameiGUNB_CLOC7
    AccessioniPrimary (citable) accession number: P28621
    Secondary accession number(s): D9SUK1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Up to 32 AA of the N-terminus and 52 AA of the C-terminus are not required for catalytic activity.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3