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Protein

Endoglucanase B

Gene

engB

Organism
Clostridium cellulovorans (strain ATCC 35296 / DSM 3052 / OCM 3 / 743B)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Has endoglucanase activity on carboxymethyl-cellulose (CMC), xylan and lichenan, but not Avicel.

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Endohydrolysis of 1,4-beta-D-xylosidic linkages in xylans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei179 – 1791Proton donorBy similarity
Active sitei305 – 3051NucleophileBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciCCEL573061:GIXD-1188-MONOMER.

Protein family/group databases

CAZyiGH5. Glycoside Hydrolase Family 5.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase B (EC:3.2.1.4)
Alternative name(s):
Cellulase B
Endo-1,4-beta-glucanase B
Gene namesi
Name:engB
Ordered Locus Names:Clocel_1150
OrganismiClostridium cellulovorans (strain ATCC 35296 / DSM 3052 / OCM 3 / 743B)
Taxonomic identifieri573061 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000002730 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3333Sequence analysisAdd
BLAST
Chaini34 – 440407Endoglucanase BPRO_0000007847Add
BLAST

Interactioni

Protein-protein interaction databases

IntActiP28621. 1 interaction.
STRINGi573061.Clocel_1150.

Structurei

3D structure databases

ProteinModelPortaliP28621.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini381 – 44060DockerinPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 dockerin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108K2F. Bacteria.
COG2730. LUCA.
HOGENOMiHOG000027405.
KOiK01179.
OMAiDSINCIN.
OrthoDBiPOG091H14IK.

Family and domain databases

Gene3Di1.10.1330.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR002105. Dockerin_1_rpt.
IPR016134. Dockerin_dom.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00150. Cellulase. 1 hit.
PF00404. Dockerin_1. 2 hits.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF63446. SSF63446. 1 hit.
PROSITEiPS00448. CLOS_CELLULOSOME_RPT. 2 hits.
PS51766. DOCKERIN. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28621-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKRLSRGKI SLLASVFVTT TFMGGVNVLA STAKTGIRDI TSQQVVKEMK
60 70 80 90 100
VGWNLGNTMD ATGGETNWGN PLTTHAMIDK VKAAGFNTLR LPITWDGHIG
110 120 130 140 150
AAPDYAIDAT WMNRVEEIAN YAFDNNMYVI INLHHEDGWL KPYYANEAEV
160 170 180 190 200
KAKITKVWTQ IANRFKDYGD YLIFETMNEP RPVGAADEWS GGSYENRDMV
210 220 230 240 250
NRYNLTAVNT IRATGGNNAL RHIMVPTLAA AALSTTMNDY IVPNNDSRVI
260 270 280 290 300
VSLHMYSPYF FSADLTSQWT TATWGSDADK AALSADFDAV YNKFVKNGRA
310 320 330 340 350
VVIGEMGTIN KNNLDSRVKH AEYYAKEATV RGITPIWWDN GYCVAGKEQT
360 370 380 390 400
FGIFNRKNLT WCCPEVMQAF IRGAGATQTQ TSYSLGDVNK DGKVNAIDYA
410 420 430 440
VLKSILLGTN TNVDLSVSDM NKDGKVNALD LAVLKKMLLS
Length:440
Mass (Da):48,637
Last modified:December 1, 1992 - v1
Checksum:i93D6CB9EA7E2AF21
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M75706 Genomic DNA. Translation: AAA23231.1.
CP002160 Genomic DNA. Translation: ADL50906.1.
PIRiA44815.
RefSeqiWP_010076241.1. NC_014393.1.

Genome annotation databases

EnsemblBacteriaiADL50906; ADL50906; Clocel_1150.
KEGGiccb:Clocel_1150.
PATRICi41738611. VBICloCel81632203721_3056.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M75706 Genomic DNA. Translation: AAA23231.1.
CP002160 Genomic DNA. Translation: ADL50906.1.
PIRiA44815.
RefSeqiWP_010076241.1. NC_014393.1.

3D structure databases

ProteinModelPortaliP28621.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP28621. 1 interaction.
STRINGi573061.Clocel_1150.

Protein family/group databases

CAZyiGH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADL50906; ADL50906; Clocel_1150.
KEGGiccb:Clocel_1150.
PATRICi41738611. VBICloCel81632203721_3056.

Phylogenomic databases

eggNOGiENOG4108K2F. Bacteria.
COG2730. LUCA.
HOGENOMiHOG000027405.
KOiK01179.
OMAiDSINCIN.
OrthoDBiPOG091H14IK.

Enzyme and pathway databases

BioCyciCCEL573061:GIXD-1188-MONOMER.

Family and domain databases

Gene3Di1.10.1330.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR002105. Dockerin_1_rpt.
IPR016134. Dockerin_dom.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00150. Cellulase. 1 hit.
PF00404. Dockerin_1. 2 hits.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF63446. SSF63446. 1 hit.
PROSITEiPS00448. CLOS_CELLULOSOME_RPT. 2 hits.
PS51766. DOCKERIN. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGUNB_CLOC7
AccessioniPrimary (citable) accession number: P28621
Secondary accession number(s): D9SUK1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: September 7, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Up to 32 AA of the N-terminus and 52 AA of the C-terminus are not required for catalytic activity.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.