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Protein

Glutamine synthetase

Gene

glnA

Organism
Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) (Agmenellum quadruplicatum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in nitrogen metabolism via ammonium assimilation. Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia.By similarity

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.By similarity

Cofactori

Mg2+By similarityNote: Binds 2 Mg2+ ions per subunit.By similarity

Enzyme regulationi

The activity of this enzyme could be controlled by adenylation under conditions of abundant glutamine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi132Magnesium 1By similarity1
Metal bindingi134Magnesium 2By similarity1
Binding sitei210ATPBy similarity1
Metal bindingi215Magnesium 2By similarity1
Metal bindingi223Magnesium 2By similarity1
Binding sitei268L-glutamate; via carbonyl oxygenBy similarity1
Metal bindingi272Magnesium 1; via pros nitrogenBy similarity1
Binding sitei276ATPBy similarity1
Binding sitei324L-glutamateBy similarity1
Binding sitei330L-glutamateBy similarity1
Binding sitei342ATPBy similarity1
Binding sitei342L-glutamateBy similarity1
Binding sitei347ATPBy similarity1
Binding sitei356ATPBy similarity1
Metal bindingi361Magnesium 1By similarity1
Binding sitei363L-glutamateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi274 – 276ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetaseBy similarity (EC:6.3.1.2By similarity)
Short name:
GSBy similarity
Alternative name(s):
Glutamate--ammonia ligaseBy similarity
Glutamine synthetase I betaBy similarity
Short name:
GSI betaBy similarity
Gene namesi
Name:glnABy similarity
Ordered Locus Names:SYNPCC7002_A1630
OrganismiSynechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) (Agmenellum quadruplicatum)
Taxonomic identifieri32049 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesSynechococcaceaeSynechococcus
Proteomesi
  • UP000001688 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001532711 – 473Glutamine synthetaseAdd BLAST473

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei401O-AMP-tyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Oligomer of 12 subunits arranged in the form of two hexagons.By similarity

Protein-protein interaction databases

STRINGi32049.SYNPCC7002_A1630.

Structurei

3D structure databases

ProteinModelPortaliP28605.
SMRiP28605.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni267 – 268L-glutamate bindingBy similarity2

Sequence similaritiesi

Belongs to the glutamine synthetase family.By similarity

Phylogenomic databases

eggNOGiENOG4105C5F. Bacteria.
COG0174. LUCA.
HOGENOMiHOG000005157.
KOiK01915.
OMAiKVLNQVG.
OrthoDBiPOG091H02IE.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.

Sequencei

Sequence statusi: Complete.

P28605-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQTATDVLR LIQEENIQII DLKFVDLPGI WQHCSFYQDQ LDEASFVDGV
60 70 80 90 100
PFDGSSIRGW KAINESDMAM VPDPTTAWID PFCKEKTLSL ICSIKEPRTG
110 120 130 140 150
EWYSRDPRSI AQKAVDYLAA SGIGDTAYFG PEAEFFVFDD VRFDQTENKG
160 170 180 190 200
FYYVDSVEGR WNSGRKEPGG NLAHKPGYKQ GYFPVPPTDT LQDMRTEMLL
210 220 230 240 250
TMAKCGVPIE KHHHEVATGG QNELGFRFAT LLKAADYLMT YKYVIKNVAR
260 270 280 290 300
KYGRTVTFMP KPLFNDNGSG MHTHQSLWKE GQPLFWGDRY ANLSQLALHY
310 320 330 340 350
IGGILKHAPA LLAFSNPSTN SYKRLVPGFE APVNLAYSQG NRSASVRIPL
360 370 380 390 400
SGPNPKAKRL EFRCPDATAN PYLAFAAMLC AGIDGIKNAI DPGDPLDVDI
410 420 430 440 450
YDLTPEELSK IPSTPASLEA ALEALQQDHD FLTAGGVFTT DFIENWIEYK
460 470
LDAEVNPLRL RPHPYEFALY YDC
Length:473
Mass (Da):53,004
Last modified:June 10, 2008 - v2
Checksum:i62323C804178884B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti120A → P in CAA78366 (PubMed:7678591).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z13965 Genomic DNA. Translation: CAA78366.1.
CP000951 Genomic DNA. Translation: ACA99620.1.
RefSeqiWP_012307243.1. NC_010475.1.

Genome annotation databases

EnsemblBacteriaiACA99620; ACA99620; SYNPCC7002_A1630.
KEGGisyp:SYNPCC7002_A1630.
PATRICi23817880. VBISynSp37135_1883.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z13965 Genomic DNA. Translation: CAA78366.1.
CP000951 Genomic DNA. Translation: ACA99620.1.
RefSeqiWP_012307243.1. NC_010475.1.

3D structure databases

ProteinModelPortaliP28605.
SMRiP28605.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi32049.SYNPCC7002_A1630.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACA99620; ACA99620; SYNPCC7002_A1630.
KEGGisyp:SYNPCC7002_A1630.
PATRICi23817880. VBISynSp37135_1883.

Phylogenomic databases

eggNOGiENOG4105C5F. Bacteria.
COG0174. LUCA.
HOGENOMiHOG000005157.
KOiK01915.
OMAiKVLNQVG.
OrthoDBiPOG091H02IE.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLN1B_SYNP2
AccessioniPrimary (citable) accession number: P28605
Secondary accession number(s): B1XNU5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: June 10, 2008
Last modified: March 15, 2017
This is version 97 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.