P28602 (HEMH_BRAJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ferrochelatase
EC=4.99.1.1

Alternative name(s):
Heme synthase
Protoheme ferro-lyase

Gene names

Name:	hemH
Ordered Locus Names:	bll7752

Organism

Bradyrhizobium japonicum

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ferrous insertion into protoporphyrin IX. Essential for normal nodule development. HAMAP MF_00323
Catalytic activity	Protoheme + 2 H+ = protoporphyrin + Fe2+. HAMAP MF_00323
Pathway	Porphyrin metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1. HAMAP MF_00323
Subcellular location	Cytoplasm By similarity HAMAP MF_00323.
Sequence similarities	Belongs to the ferrochelatase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferrochelatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	Ferrochelatase HAMAP MF_00323		PRO_0000175119
Sites
	Metal binding	215	1	Iron By similarity
	Metal binding	296	1	Iron By similarity
Experimental info
	Sequence conflict	15	1	Q → R in AAA26217. Ref.1
	Sequence conflict	75	1	T → S in AAA26217. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P28602 [UniParc]. Last modified February 28, 2003. Version 3. Checksum: 67833BFE08E0784C	FASTA	345	38,418
	10 20 30 40 50 60 MSTAAPNETT QPTVQSGQKR VGVLLVNLGT PDTADAPGVR VYLKEFLSDA RVIEDQGLVW 70 80 90 100 110 120 KVVLNGIILR SRPRTKALDY QKIWNNEKNE SPLKTITRSQ SDKLAAALSD RDHVVVDWAM 130 140 150 160 170 180 RYGNPSIKSG IDALIAEGCD RILAVPLYPQ YSASTSATVC DEVFRVLARL RAQPTLRVTP 190 200 210 220 230 240 PYYEDEAYIE ALAVSIETHL ATLPFKPELI VASFHGMPKS YVDKGDPYQE HCIATTEALR 250 260 270 280 290 300 RRLGVDASKL LLTFQSRFGN DEWLQPYTDK TMERLAKEGV RRIAVVTPGF AADCLETLEE 310 320 330 340 IAQENAEIFK HNGGEQFSAI PCLNDSEPGM DVIRTLVLRE LQGWI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of a Bradyrhizobium japonicum ferrochelatase mutant and isolation of the hemH gene." Frustaci J.M., O'Brian M.R. J. Bacteriol. 174:4223-4229(1992) [PubMed: 1624416] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LO.
[2]	O'Brian M. Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M92427 Genomic DNA. Translation: AAA26217.2. BA000040 Genomic DNA. Translation: BAC53017.1.
PIR	A42883.
RefSeq	NP_774392.1. NC_004463.1.
3D structure databases
ProteinModelPortal	P28602.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1047995.
GenomeReviews	Gene locus bll7752 in contig BA000040_GR.
KEGG	bja:bll7752.
NMPDR	fig|224911.1.peg.7752.
PATRIC	21199492. VBIBraJap65052_7974.
Phylogenomic databases
HOGENOM	HBG697135.
OMA	TIEEIGM.
PhylomeDB	P28602.
ProtClustDB	PRK00035.
Enzyme and pathway databases
BioCyc	BJAP224911:BLL7752-MONOMER.
Family and domain databases
HAMAP	MF_00323. Ferrochelatase. [Tree]
InterPro	IPR001015. Ferrochelatase. IPR019772. Ferrochelatase_AS. [Graphical view]
KO	K01772.
PANTHER	PTHR11108. Ferrochelatase. 1 hit.
Pfam	PF00762. Ferrochelatase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00109. HemH. 1 hit.
PROSITE	PS00534. FERROCHELATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMH_BRAJA

Accession

Primary (citable) accession number: P28602

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	February 28, 2003
Last modified:	January 25, 2012
This is version 84 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program