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P28585 (BLC1_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-lactamase CTX-M-1

EC=3.5.2.6
Alternative name(s):
Beta-lactamase MEN-1
Cefotaximase 1
Gene names
Name:bla
Synonyms:men1
Encoded onPlasmid pMVP-3
OrganismEscherichia coli
Taxonomic identifier562 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length291 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Broad spectrum beta-lactamase which confers resistance to penicillins, as well as first, second and third-generation cephalosporins.

Catalytic activity

A beta-lactam + H2O = a substituted beta-amino acid.

Sequence similarities

Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
   Biological processAntibiotic resistance
   DomainSignal
   Molecular functionHydrolase
   Technical termDirect protein sequencing
Plasmid
Gene Ontology (GO)
   Biological_processbeta-lactam antibiotic catabolic process

Inferred from electronic annotation. Source: InterPro

response to antibiotic

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionbeta-lactamase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2828 Ref.2
Chain29 – 291263Beta-lactamase CTX-M-1
PRO_0000016989

Regions

Region237 – 2393Substrate binding By similarity

Sites

Active site731Acyl-ester intermediate By similarity

Sequences

Sequence LengthMass (Da)Tools
P28585 [UniParc].

Last modified December 15, 1998. Version 2.
Checksum: D82501CF8AE55FC5

FASTA29131,246
        10         20         30         40         50         60 
MVKKSLRQFT LMATATVTLL LGSVPLYAQT ADVQQKLAEL ERQSGGRLGV ALINTADNSQ 

        70         80         90        100        110        120 
ILYRADERFA MCSTSKVMAV AAVLKKSESE PNLLNQRVEI KKSDLVNYNP IAEKHVDGTM 

       130        140        150        160        170        180 
SLAELSAAAL QYSDNVAMNK LISHVGGPAS VTAFARQLGD ETFRLDRTEP TLNTAIPGDP 

       190        200        210        220        230        240 
RDTTSPRAMA QTLRNLTLGK ALGDSQRAQL VTWMKGNTTG AASIQAGLPA SWVVGDKTGS 

       250        260        270        280        290 
GDYGTTNDIA VIWPKDRAPL ILVTYFTQPQ PKAESRRDVL ASAAKIVTNG L 

« Hide

References

[1]"Sequences of beta-lactamase genes encoding CTX-M-1 (MEN-1) and CTX-M-2 and relationship of their amino acid sequences with those of other beta-lactamases."
Bauernfeind A., Stemplinger I., Jungwirth R., Casellas J.M.
Antimicrob. Agents Chemother. 40:509-513(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: GRI-1.
[2]"Close amino acid sequence relationship between the new plasmid-mediated extended-spectrum beta-lactamase MEN-1 and chromosomally encoded enzymes of Klebsiella oxytoca."
Barthelemy M., Peduzzi J., Bernard H., Tancrede C., Labia R.
Biochim. Biophys. Acta 1122:15-22(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 29-291.
Strain: MEN.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X92506 Genomic DNA. Translation: CAA63262.1.
PIRS23929.
RefSeqYP_006954479.1. NC_019098.1.
YP_008995260.1. NC_023277.1.
YP_008997418.1. NC_023289.1.

3D structure databases

ProteinModelPortalP28585.
SMRP28585. Positions 33-291.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBP28585.

Proteomic databases

PRIDEP28585.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID13909205.
18157735.
18157941.

Phylogenomic databases

ProtClustDBCLSK522521.

Family and domain databases

Gene3D3.40.710.10. 1 hit.
InterProIPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PfamPF00144. Beta-lactamase. 1 hit.
[Graphical view]
PRINTSPR00118. BLACTAMASEA.
SUPFAMSSF56601. SSF56601. 1 hit.
PROSITEPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBLC1_ECOLX
AccessionPrimary (citable) accession number: P28585
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 15, 1998
Last modified: March 19, 2014
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families