P28585 (BLC1_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-lactamase CTX-M-1
EC=3.5.2.6

Alternative name(s):
Beta-lactamase MEN-1
Cefotaximase 1

Gene names

Name:	bla
Synonyms:	men1

Encoded on

Plasmid pMVP-3

Organism

Escherichia coli

Taxonomic identifier

562 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Broad spectrum beta-lactamase which confers resistance to penicillins, as well as first, second and third-generation cephalosporins.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Molecular function	Hydrolase
Technical term	Direct protein sequencing Plasmid
Gene Ontology (GO)
Biological_process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Ref.2
	Chain	29 – 291	263	Beta-lactamase CTX-M-1		PRO_0000016989
Regions
	Region	237 – 239	3	Substrate binding By similarity
Sites
	Active site	73	1	Acyl-ester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P28585 [UniParc]. Last modified December 15, 1998. Version 2. Checksum: D82501CF8AE55FC5	FASTA	291	31,246
	10 20 30 40 50 60 MVKKSLRQFT LMATATVTLL LGSVPLYAQT ADVQQKLAEL ERQSGGRLGV ALINTADNSQ 70 80 90 100 110 120 ILYRADERFA MCSTSKVMAV AAVLKKSESE PNLLNQRVEI KKSDLVNYNP IAEKHVDGTM 130 140 150 160 170 180 SLAELSAAAL QYSDNVAMNK LISHVGGPAS VTAFARQLGD ETFRLDRTEP TLNTAIPGDP 190 200 210 220 230 240 RDTTSPRAMA QTLRNLTLGK ALGDSQRAQL VTWMKGNTTG AASIQAGLPA SWVVGDKTGS 250 260 270 280 290 GDYGTTNDIA VIWPKDRAPL ILVTYFTQPQ PKAESRRDVL ASAAKIVTNG L

References

[1]	"Sequences of beta-lactamase genes encoding CTX-M-1 (MEN-1) and CTX-M-2 and relationship of their amino acid sequences with those of other beta-lactamases." Bauernfeind A., Stemplinger I., Jungwirth R., Casellas J.M. Antimicrob. Agents Chemother. 40:509-513(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: GRI-1.
[2]	"Close amino acid sequence relationship between the new plasmid-mediated extended-spectrum beta-lactamase MEN-1 and chromosomally encoded enzymes of Klebsiella oxytoca." Barthelemy M., Peduzzi J., Bernard H., Tancrede C., Labia R. Biochim. Biophys. Acta 1122:15-22(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 29-291. Strain: MEN.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X92506 Genomic DNA. Translation: CAA63262.1.
PIR	S23929.
RefSeq	YP_006954479.1. NC_019098.1.
3D structure databases
ProteinModelPortal	P28585.
SMR	P28585. Positions 33-291.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	13909205.
Phylogenomic databases
ProtClustDB	CLSK522521.
Family and domain databases
Gene3D	3.40.710.10. 1 hit.
InterPro	IPR001466. Beta-lactam-related. IPR012338. Beta-lactam/transpept-like. IPR000871. Beta-lactam_class-A/D. IPR023650. Beta-lactam_class-A_AS. [Graphical view]
Pfam	PF00144. Beta-lactamase. 1 hit. [Graphical view]
PRINTS	PR00118. BLACTAMASEA.
SUPFAM	SSF56601. PBP_transp_fold. 1 hit.
PROSITE	PS00146. BETA_LACTAMASE_A. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P28585.

Entry information

Entry name

BLC1_ECOLX

Accession

Primary (citable) accession number: P28585

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 15, 1998
Last modified:	April 3, 2013
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program