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P28585

- BLC1_ECOLX

UniProt

P28585 - BLC1_ECOLX

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Protein

Beta-lactamase CTX-M-1

Gene

bla

Organism
Escherichia coli
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Broad spectrum beta-lactamase which confers resistance to penicillins, as well as first, second and third-generation cephalosporins.

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei73 – 731Acyl-ester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. beta-lactamase activity Source: UniProtKB-EC

GO - Biological processi

  1. beta-lactam antibiotic catabolic process Source: InterPro
  2. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase CTX-M-1 (EC:3.5.2.6)
Alternative name(s):
Beta-lactamase MEN-1
Cefotaximase 1
Gene namesi
Name:bla
Synonyms:men1
Encoded oniPlasmid pMVP-30 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 28281 PublicationAdd
BLAST
Chaini29 – 291263Beta-lactamase CTX-M-1PRO_0000016989Add
BLAST

Proteomic databases

PRIDEiP28585.

Interactioni

Structurei

3D structure databases

ProteinModelPortaliP28585.
SMRiP28585. Positions 33-291.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni237 – 2393Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the class-A beta-lactamase family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PfamiPF00144. Beta-lactamase. 1 hit.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28585-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVKKSLRQFT LMATATVTLL LGSVPLYAQT ADVQQKLAEL ERQSGGRLGV
60 70 80 90 100
ALINTADNSQ ILYRADERFA MCSTSKVMAV AAVLKKSESE PNLLNQRVEI
110 120 130 140 150
KKSDLVNYNP IAEKHVDGTM SLAELSAAAL QYSDNVAMNK LISHVGGPAS
160 170 180 190 200
VTAFARQLGD ETFRLDRTEP TLNTAIPGDP RDTTSPRAMA QTLRNLTLGK
210 220 230 240 250
ALGDSQRAQL VTWMKGNTTG AASIQAGLPA SWVVGDKTGS GDYGTTNDIA
260 270 280 290
VIWPKDRAPL ILVTYFTQPQ PKAESRRDVL ASAAKIVTNG L
Length:291
Mass (Da):31,246
Last modified:December 15, 1998 - v2
Checksum:iD82501CF8AE55FC5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X92506 Genomic DNA. Translation: CAA63262.1.
PIRiS23929.
RefSeqiWP_013188473.1. NC_023329.1.
YP_006954479.1. NC_019098.1.
YP_008995260.1. NC_023277.2.
YP_008997418.1. NC_023289.2.
YP_008998816.1. NC_023329.1.

Genome annotation databases

GeneIDi13909205.
18157735.
18157940.
18253700.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X92506 Genomic DNA. Translation: CAA63262.1 .
PIRi S23929.
RefSeqi WP_013188473.1. NC_023329.1.
YP_006954479.1. NC_019098.1.
YP_008995260.1. NC_023277.2.
YP_008997418.1. NC_023289.2.
YP_008998816.1. NC_023329.1.

3D structure databases

ProteinModelPortali P28585.
SMRi P28585. Positions 33-291.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi P28585.

Proteomic databases

PRIDEi P28585.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 13909205.
18157735.
18157940.
18253700.

Family and domain databases

Gene3Di 3.40.710.10. 1 hit.
InterProi IPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view ]
Pfami PF00144. Beta-lactamase. 1 hit.
[Graphical view ]
PRINTSi PR00118. BLACTAMASEA.
SUPFAMi SSF56601. SSF56601. 1 hit.
PROSITEi PS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequences of beta-lactamase genes encoding CTX-M-1 (MEN-1) and CTX-M-2 and relationship of their amino acid sequences with those of other beta-lactamases."
    Bauernfeind A., Stemplinger I., Jungwirth R., Casellas J.M.
    Antimicrob. Agents Chemother. 40:509-513(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: GRI-1.
  2. "Close amino acid sequence relationship between the new plasmid-mediated extended-spectrum beta-lactamase MEN-1 and chromosomally encoded enzymes of Klebsiella oxytoca."
    Barthelemy M., Peduzzi J., Bernard H., Tancrede C., Labia R.
    Biochim. Biophys. Acta 1122:15-22(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 29-291.
    Strain: MEN.

Entry informationi

Entry nameiBLC1_ECOLX
AccessioniPrimary (citable) accession number: P28585
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 15, 1998
Last modified: October 29, 2014
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing, Plasmid

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3