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P28585

- BLC1_ECOLX

UniProt

P28585 - BLC1_ECOLX

Protein

Beta-lactamase CTX-M-1

Gene

bla

Organism
Escherichia coli
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 2 (15 Dec 1998)
      Previous versions | rss
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    Functioni

    Broad spectrum beta-lactamase which confers resistance to penicillins, as well as first, second and third-generation cephalosporins.

    Catalytic activityi

    A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei73 – 731Acyl-ester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. beta-lactamase activity Source: UniProtKB-EC

    GO - Biological processi

    1. beta-lactam antibiotic catabolic process Source: InterPro
    2. response to antibiotic Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Antibiotic resistance

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-lactamase CTX-M-1 (EC:3.5.2.6)
    Alternative name(s):
    Beta-lactamase MEN-1
    Cefotaximase 1
    Gene namesi
    Name:bla
    Synonyms:men1
    Encoded oniPlasmid pMVP-30 Publication
    OrganismiEscherichia coli
    Taxonomic identifieri562 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 28281 PublicationAdd
    BLAST
    Chaini29 – 291263Beta-lactamase CTX-M-1PRO_0000016989Add
    BLAST

    Proteomic databases

    PRIDEiP28585.

    Interactioni

    Structurei

    3D structure databases

    ProteinModelPortaliP28585.
    SMRiP28585. Positions 33-291.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni237 – 2393Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the class-A beta-lactamase family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    InterProiIPR001466. Beta-lactam-related.
    IPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    IPR023650. Beta-lactam_class-A_AS.
    [Graphical view]
    PfamiPF00144. Beta-lactamase. 1 hit.
    [Graphical view]
    PRINTSiPR00118. BLACTAMASEA.
    SUPFAMiSSF56601. SSF56601. 1 hit.
    PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P28585-1 [UniParc]FASTAAdd to Basket

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    MVKKSLRQFT LMATATVTLL LGSVPLYAQT ADVQQKLAEL ERQSGGRLGV    50
    ALINTADNSQ ILYRADERFA MCSTSKVMAV AAVLKKSESE PNLLNQRVEI 100
    KKSDLVNYNP IAEKHVDGTM SLAELSAAAL QYSDNVAMNK LISHVGGPAS 150
    VTAFARQLGD ETFRLDRTEP TLNTAIPGDP RDTTSPRAMA QTLRNLTLGK 200
    ALGDSQRAQL VTWMKGNTTG AASIQAGLPA SWVVGDKTGS GDYGTTNDIA 250
    VIWPKDRAPL ILVTYFTQPQ PKAESRRDVL ASAAKIVTNG L 291
    Length:291
    Mass (Da):31,246
    Last modified:December 15, 1998 - v2
    Checksum:iD82501CF8AE55FC5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X92506 Genomic DNA. Translation: CAA63262.1.
    PIRiS23929.
    RefSeqiWP_013188473.1. NC_019098.1.
    YP_006954479.1. NC_019098.1.
    YP_008995260.1. NC_023277.2.
    YP_008997418.1. NC_023289.2.
    YP_008998816.1. NC_023329.1.

    Genome annotation databases

    GeneIDi13909205.
    18157735.
    18157940.
    18253700.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X92506 Genomic DNA. Translation: CAA63262.1 .
    PIRi S23929.
    RefSeqi WP_013188473.1. NC_019098.1.
    YP_006954479.1. NC_019098.1.
    YP_008995260.1. NC_023277.2.
    YP_008997418.1. NC_023289.2.
    YP_008998816.1. NC_023329.1.

    3D structure databases

    ProteinModelPortali P28585.
    SMRi P28585. Positions 33-291.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi P28585.

    Proteomic databases

    PRIDEi P28585.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 13909205.
    18157735.
    18157940.
    18253700.

    Family and domain databases

    Gene3Di 3.40.710.10. 1 hit.
    InterProi IPR001466. Beta-lactam-related.
    IPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    IPR023650. Beta-lactam_class-A_AS.
    [Graphical view ]
    Pfami PF00144. Beta-lactamase. 1 hit.
    [Graphical view ]
    PRINTSi PR00118. BLACTAMASEA.
    SUPFAMi SSF56601. SSF56601. 1 hit.
    PROSITEi PS00146. BETA_LACTAMASE_A. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequences of beta-lactamase genes encoding CTX-M-1 (MEN-1) and CTX-M-2 and relationship of their amino acid sequences with those of other beta-lactamases."
      Bauernfeind A., Stemplinger I., Jungwirth R., Casellas J.M.
      Antimicrob. Agents Chemother. 40:509-513(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: GRI-1.
    2. "Close amino acid sequence relationship between the new plasmid-mediated extended-spectrum beta-lactamase MEN-1 and chromosomally encoded enzymes of Klebsiella oxytoca."
      Barthelemy M., Peduzzi J., Bernard H., Tancrede C., Labia R.
      Biochim. Biophys. Acta 1122:15-22(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 29-291.
      Strain: MEN.

    Entry informationi

    Entry nameiBLC1_ECOLX
    AccessioniPrimary (citable) accession number: P28585
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: December 15, 1998
    Last modified: October 1, 2014
    This is version 79 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing, Plasmid

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3