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Protein

Calcium-dependent protein kinase SK5

Gene
N/A
Organism
Glycine max (Soybean) (Glycine hispida)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in signal transduction pathways that involve calcium as a second messenger.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by calcium. Autophosphorylation may play an important role in the regulation of the kinase activity (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei63 – 631ATPPROSITE-ProRule annotation
Active sitei158 – 1581Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi40 – 489ATPPROSITE-ProRule annotation
Calcium bindingi348 – 359121Add
BLAST
Calcium bindingi384 – 395122Add
BLAST
Calcium bindingi420 – 431123Add
BLAST
Calcium bindingi454 – 465124Add
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 2483.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-dependent protein kinase SK5 (EC:2.7.11.1)
Short name:
CDPK
OrganismiGlycine max (Soybean) (Glycine hispida)
Taxonomic identifieri3847 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeGlycineSoja
Proteomesi
  • UP000008827 Componenti: Chromosome 8

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 508508Calcium-dependent protein kinase SK5PRO_0000085833Add
BLAST

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP28583.

Expressioni

Tissue specificityi

Found throughout the plant.

Gene expression databases

GenevisibleiP28583. GM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi3847.GLYMA08G00840.1.

Structurei

Secondary structure

1
508
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi331 – 3333Combined sources
Turni335 – 3373Combined sources
Helixi340 – 3456Combined sources
Beta strandi348 – 3536Combined sources
Helixi357 – 3648Combined sources
Turni365 – 3684Combined sources
Helixi373 – 38210Combined sources
Beta strandi388 – 3914Combined sources
Helixi393 – 4008Combined sources
Beta strandi403 – 4064Combined sources
Helixi413 – 4186Combined sources
Turni419 – 4224Combined sources
Beta strandi424 – 4285Combined sources
Helixi429 – 4346Combined sources
Turni435 – 4406Combined sources
Helixi445 – 4539Combined sources
Beta strandi455 – 4628Combined sources
Helixi465 – 4684Combined sources
Beta strandi483 – 4853Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1S6INMR-A329-508[»]
1S6JNMR-A329-402[»]
DisProtiDP00561.
ProteinModelPortaliP28583.
SMRiP28583. Positions 329-508.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP28583.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini34 – 292259Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini335 – 37036EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini371 – 40636EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini407 – 44236EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini443 – 47634EF-hand 4PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni298 – 32831Autoinhibitory domainBy similarityAdd
BLAST

Domaini

There is 3 contiguous domains conserved in the CDPK subfamily: a kinase domain, an autoinhibitory (junction) domain and a calmodulin-like domain. The autoinhibitory domain (298-328) inactivates kinase activity under calcium-free conditions (By similarity).By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.PROSITE-ProRule annotation
Contains 4 EF-hand domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
InParanoidiP28583.
KOiK13412.
OMAiMANKPRT.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28583-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAKSSSSST TTNVVTLKAA WVLPQRTQNI REVYEVGRKL GQGQFGTTFE
60 70 80 90 100
CTRRASGGKF ACKSIPKRKL LCKEDYEDVW REIQIMHHLS EHANVVRIEG
110 120 130 140 150
TYEDSTAVHL VMELCEGGEL FDRIVQKGHY SERQAARLIK TIVEVVEACH
160 170 180 190 200
SLGVMHRDLK PENFLFDTID EDAKLKATDF GLSVFYKPGE SFCDVVGSPY
210 220 230 240 250
YVAPEVLRKL YGPESDVWSA GVILYILLSG VPPFWAESEP GIFRQILLGK
260 270 280 290 300
LDFHSEPWPS ISDSAKDLIR KMLDQNPKTR LTAHEVLRHP WIVDDNIAPD
310 320 330 340 350
KPLDSAVLSR LKQFSAMNKL KKMALRVIAE RLSEEEIGGL KELFKMIDTD
360 370 380 390 400
NSGTITFDEL KDGLKRVGSE LMESEIKDLM DAADIDKSGT IDYGEFIAAT
410 420 430 440 450
VHLNKLEREE NLVSAFSYFD KDGSGYITLD EIQQACKDFG LDDIHIDDMI
460 470 480 490 500
KEIDQDNDGQ IDYGEFAAMM RKGNGGIGRR TMRKTLNLRD ALGLVDNGSN

QVIEGYFK
Length:508
Mass (Da):57,169
Last modified:December 1, 1992 - v1
Checksum:iAFCEDC51224192E4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64987 mRNA. Translation: AAB00806.1.
PIRiA43713.
RefSeqiNP_001238517.2. NM_001251588.2.
UniGeneiGma.8437.

Genome annotation databases

EnsemblPlantsiGLYMA08G00840.1; GLYMA08G00840.1; GLYMA08G00840.
GeneIDi547825.
GrameneiGLYMA08G00840.1; GLYMA08G00840.1; GLYMA08G00840.
KEGGigmx:547825.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64987 mRNA. Translation: AAB00806.1.
PIRiA43713.
RefSeqiNP_001238517.2. NM_001251588.2.
UniGeneiGma.8437.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1S6INMR-A329-508[»]
1S6JNMR-A329-402[»]
DisProtiDP00561.
ProteinModelPortaliP28583.
SMRiP28583. Positions 329-508.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3847.GLYMA08G00840.1.

Proteomic databases

PRIDEiP28583.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiGLYMA08G00840.1; GLYMA08G00840.1; GLYMA08G00840.
GeneIDi547825.
GrameneiGLYMA08G00840.1; GLYMA08G00840.1; GLYMA08G00840.
KEGGigmx:547825.

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
InParanoidiP28583.
KOiK13412.
OMAiMANKPRT.

Enzyme and pathway databases

BRENDAi2.7.11.1. 2483.

Miscellaneous databases

EvolutionaryTraceiP28583.

Gene expression databases

GenevisibleiP28583. GM.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A calcium-dependent protein kinase with a regulatory domain similar to calmodulin."
    Harper J.F., Sussman M.R., Schaller G.E., Putnam-Evans C., Charbonneau H., Harmon A.C.
    Science 252:951-954(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Williams.
  2. "Unexpected structure of the Ca(2+)-regulatory region from soybean calcium-dependent protein kinase-alpha."
    Weljie A.M., Vogel H.J.
    J. Biol. Chem. 279:35494-35502(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 329-508 IN COMPLEX WITH CALCIUM ION.

Entry informationi

Entry nameiCDPK_SOYBN
AccessioniPrimary (citable) accession number: P28583
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: June 8, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.