Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Calcium-dependent protein kinase SK5

Gene
N/A
Organism
Glycine max (Soybean) (Glycine hispida)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in signal transduction pathways that involve calcium as a second messenger.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by calcium. Autophosphorylation may play an important role in the regulation of the kinase activity (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei63ATPPROSITE-ProRule annotation1
Active sitei158Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi40 – 48ATPPROSITE-ProRule annotation9
Calcium bindingi348 – 3591Add BLAST12
Calcium bindingi384 – 3952Add BLAST12
Calcium bindingi420 – 4313Add BLAST12
Calcium bindingi454 – 4654Add BLAST12

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 2483.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-dependent protein kinase SK5 (EC:2.7.11.1)
Short name:
CDPK
OrganismiGlycine max (Soybean) (Glycine hispida)
Taxonomic identifieri3847 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeGlycineSoja
Proteomesi
  • UP000008827 Componenti: Chromosome 8

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000858331 – 508Calcium-dependent protein kinase SK5Add BLAST508

Keywords - PTMi

Phosphoprotein

Expressioni

Tissue specificityi

Found throughout the plant.

Gene expression databases

GenevisibleiP28583. GM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi3847.GLYMA08G00840.1.

Structurei

Secondary structure

1508
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi331 – 333Combined sources3
Turni335 – 337Combined sources3
Helixi340 – 345Combined sources6
Beta strandi348 – 353Combined sources6
Helixi357 – 364Combined sources8
Turni365 – 368Combined sources4
Helixi373 – 382Combined sources10
Beta strandi388 – 391Combined sources4
Helixi393 – 400Combined sources8
Beta strandi403 – 406Combined sources4
Helixi413 – 418Combined sources6
Turni419 – 422Combined sources4
Beta strandi424 – 428Combined sources5
Helixi429 – 434Combined sources6
Turni435 – 440Combined sources6
Helixi445 – 453Combined sources9
Beta strandi455 – 462Combined sources8
Helixi465 – 468Combined sources4
Beta strandi483 – 485Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1S6INMR-A329-508[»]
1S6JNMR-A329-402[»]
DisProtiDP00561.
ProteinModelPortaliP28583.
SMRiP28583.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP28583.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini34 – 292Protein kinasePROSITE-ProRule annotationAdd BLAST259
Domaini335 – 370EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini371 – 406EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini407 – 442EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini443 – 476EF-hand 4PROSITE-ProRule annotationAdd BLAST34

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni298 – 328Autoinhibitory domainBy similarityAdd BLAST31

Domaini

There is 3 contiguous domains conserved in the CDPK subfamily: a kinase domain, an autoinhibitory (junction) domain and a calmodulin-like domain. The autoinhibitory domain (298-328) inactivates kinase activity under calcium-free conditions (By similarity).By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.PROSITE-ProRule annotation
Contains 4 EF-hand domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
InParanoidiP28583.
KOiK13412.
OMAiSQACHEF.
OrthoDBiEOG093609BM.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28583-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAKSSSSST TTNVVTLKAA WVLPQRTQNI REVYEVGRKL GQGQFGTTFE
60 70 80 90 100
CTRRASGGKF ACKSIPKRKL LCKEDYEDVW REIQIMHHLS EHANVVRIEG
110 120 130 140 150
TYEDSTAVHL VMELCEGGEL FDRIVQKGHY SERQAARLIK TIVEVVEACH
160 170 180 190 200
SLGVMHRDLK PENFLFDTID EDAKLKATDF GLSVFYKPGE SFCDVVGSPY
210 220 230 240 250
YVAPEVLRKL YGPESDVWSA GVILYILLSG VPPFWAESEP GIFRQILLGK
260 270 280 290 300
LDFHSEPWPS ISDSAKDLIR KMLDQNPKTR LTAHEVLRHP WIVDDNIAPD
310 320 330 340 350
KPLDSAVLSR LKQFSAMNKL KKMALRVIAE RLSEEEIGGL KELFKMIDTD
360 370 380 390 400
NSGTITFDEL KDGLKRVGSE LMESEIKDLM DAADIDKSGT IDYGEFIAAT
410 420 430 440 450
VHLNKLEREE NLVSAFSYFD KDGSGYITLD EIQQACKDFG LDDIHIDDMI
460 470 480 490 500
KEIDQDNDGQ IDYGEFAAMM RKGNGGIGRR TMRKTLNLRD ALGLVDNGSN

QVIEGYFK
Length:508
Mass (Da):57,169
Last modified:December 1, 1992 - v1
Checksum:iAFCEDC51224192E4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64987 mRNA. Translation: AAB00806.1.
PIRiA43713.
RefSeqiNP_001238517.2. NM_001251588.2.
UniGeneiGma.8437.

Genome annotation databases

EnsemblPlantsiGLYMA08G00840.1; GLYMA08G00840.1; GLYMA08G00840.
GeneIDi547825.
GrameneiGLYMA08G00840.1; GLYMA08G00840.1; GLYMA08G00840.
KEGGigmx:547825.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64987 mRNA. Translation: AAB00806.1.
PIRiA43713.
RefSeqiNP_001238517.2. NM_001251588.2.
UniGeneiGma.8437.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1S6INMR-A329-508[»]
1S6JNMR-A329-402[»]
DisProtiDP00561.
ProteinModelPortaliP28583.
SMRiP28583.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3847.GLYMA08G00840.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiGLYMA08G00840.1; GLYMA08G00840.1; GLYMA08G00840.
GeneIDi547825.
GrameneiGLYMA08G00840.1; GLYMA08G00840.1; GLYMA08G00840.
KEGGigmx:547825.

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
InParanoidiP28583.
KOiK13412.
OMAiSQACHEF.
OrthoDBiEOG093609BM.

Enzyme and pathway databases

BRENDAi2.7.11.1. 2483.

Miscellaneous databases

EvolutionaryTraceiP28583.

Gene expression databases

GenevisibleiP28583. GM.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCDPK_SOYBN
AccessioniPrimary (citable) accession number: P28583
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: November 30, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.