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Protein

Platelet-derived growth factor subunit A

Gene

Pdgfa

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Growth factor, Mitogen

Enzyme and pathway databases

ReactomeiR-RNO-114608. Platelet degranulation.
R-RNO-1257604. PIP3 activates AKT signaling.
R-RNO-186763. Downstream signal transduction.
R-RNO-186797. Signaling by PDGF.
R-RNO-3000171. Non-integrin membrane-ECM interactions.
R-RNO-5673001. RAF/MAP kinase cascade.
R-RNO-6811558. PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Platelet-derived growth factor subunit A
Short name:
PDGF subunit A
Alternative name(s):
PDGF-1
Platelet-derived growth factor A chain
Platelet-derived growth factor alpha polypeptide
Gene namesi
Name:Pdgfa
Synonyms:Rpa1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi3282. Pdgfa.

Subcellular locationi

  • Secreted

  • Note: Released by platelets upon wounding.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020By similarityAdd
BLAST
Propeptidei21 – 8565Removed in mature formPRO_0000023362Add
BLAST
Chaini86 – 204119Platelet-derived growth factor subunit APRO_0000023363Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi96 ↔ 140By similarity
Disulfide bondi123 – 123InterchainBy similarity
Disulfide bondi129 ↔ 177By similarity
Disulfide bondi132 – 132InterchainBy similarity
Disulfide bondi133 ↔ 179By similarity
Glycosylationi134 – 1341N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP28576.

Expressioni

Developmental stagei

In kidney epithelial tissues, the shorter form predominates in young (1 day old) rats while the longer form becomes more prevalant during aging.

Gene expression databases

BgeeiENSRNOG00000001312.
ExpressionAtlasiP28576. baseline and differential.
GenevisibleiP28576. RN.

Interactioni

Subunit structurei

Homodimer; antiparallel disulfide-linked dimer. Heterodimer with PDGFB; antiparallel disulfide-linked dimer. The PDGFA homodimer interacts with PDGFRA homodimers, and with heterodimers formed by PDGFRA and PDGFRB. The heterodimer composed of PDGFA and PDGFB interacts with PDGFRA homodimers, and with heterodimers formed by PDGFRA and PDGFRB. Interacts with CSPG4 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000001775.

Structurei

3D structure databases

ProteinModelPortaliP28576.
SMRiP28576. Positions 89-183.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni158 – 1625Receptor binding siteSequence analysis

Domaini

The long form contains a basic insert which acts as a cell retention signal.

Sequence similaritiesi

Belongs to the PDGF/VEGF growth factor family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IEXJ. Eukaryota.
ENOG4111GMH. LUCA.
GeneTreeiENSGT00510000046755.
HOVERGENiHBG053546.
InParanoidiP28576.
KOiK04359.
PhylomeDBiP28576.

Family and domain databases

Gene3Di2.10.90.10. 1 hit.
InterProiIPR029034. Cystine-knot_cytokine.
IPR023581. PD_growth_factor_CS.
IPR000072. PDGF/VEGF_dom.
IPR006782. PDGF_N.
[Graphical view]
PfamiPF00341. PDGF. 1 hit.
PF04692. PDGF_N. 1 hit.
[Graphical view]
SMARTiSM00141. PDGF. 1 hit.
[Graphical view]
SUPFAMiSSF57501. SSF57501. 1 hit.
PROSITEiPS00249. PDGF_1. 1 hit.
PS50278. PDGF_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P28576-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRTWACLLLL GCGYLAHALA EEAEIPRELI ERLARSQIHS IRDLQRLLEI
60 70 80 90 100
DSVGAEDALE TNLRAHGSHT VKHVPEKRPV PIRRKRSIEE AIPAVCKTRT
110 120 130 140 150
VIYEIPRSQV DPTSANFLIW PPCVEVKRCT GCCNTSSVKC QPSRVHHRSV
160 170 180 190 200
KVAKVEYVRK KPKLKEVQVR LEEHLECACA TSNLNPDHRE EETGRRRESG

KKRK
Length:204
Mass (Da):23,307
Last modified:February 1, 1994 - v2
Checksum:iFA413F74E86F742C
GO
Isoform Short (identifier: P28576-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     194-196: GRR → DVR
     197-204: Missing.

Show »
Length:196
Mass (Da):22,338
Checksum:i204910ED38EEA1F1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti85 – 11127KRSIE…RSQVD → REVLRKPFPQFARPGRSFTR YLGARWT in BAA00987 (PubMed:8447423).CuratedAdd
BLAST
Sequence conflicti119 – 1191I → T in AAA41932 (Ref. 3) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei194 – 1963GRR → DVR in isoform Short. 2 PublicationsVSP_004609
Alternative sequencei197 – 2048Missing in isoform Short. 2 PublicationsVSP_004610

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06894 mRNA. Translation: AAB59693.1.
Z14120 mRNA. Translation: CAA78490.1.
D10106 mRNA. Translation: BAA00987.1.
L06238 mRNA. Translation: AAA41932.1.
S57864 mRNA. Translation: AAB26134.2.
PIRiA48851.
S25096.
RefSeqiNP_036933.1. NM_012801.1.
XP_006248981.1. XM_006248919.2. [P28576-2]
UniGeneiRn.10999.

Genome annotation databases

EnsembliENSRNOT00000001775; ENSRNOP00000001775; ENSRNOG00000001312. [P28576-2]
ENSRNOT00000042117; ENSRNOP00000040116; ENSRNOG00000001312. [P28576-2]
GeneIDi25266.
KEGGirno:25266.
UCSCiRGD:3282. rat. [P28576-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06894 mRNA. Translation: AAB59693.1.
Z14120 mRNA. Translation: CAA78490.1.
D10106 mRNA. Translation: BAA00987.1.
L06238 mRNA. Translation: AAA41932.1.
S57864 mRNA. Translation: AAB26134.2.
PIRiA48851.
S25096.
RefSeqiNP_036933.1. NM_012801.1.
XP_006248981.1. XM_006248919.2. [P28576-2]
UniGeneiRn.10999.

3D structure databases

ProteinModelPortaliP28576.
SMRiP28576. Positions 89-183.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000001775.

Proteomic databases

PaxDbiP28576.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000001775; ENSRNOP00000001775; ENSRNOG00000001312. [P28576-2]
ENSRNOT00000042117; ENSRNOP00000040116; ENSRNOG00000001312. [P28576-2]
GeneIDi25266.
KEGGirno:25266.
UCSCiRGD:3282. rat. [P28576-1]

Organism-specific databases

CTDi5154.
RGDi3282. Pdgfa.

Phylogenomic databases

eggNOGiENOG410IEXJ. Eukaryota.
ENOG4111GMH. LUCA.
GeneTreeiENSGT00510000046755.
HOVERGENiHBG053546.
InParanoidiP28576.
KOiK04359.
PhylomeDBiP28576.

Enzyme and pathway databases

ReactomeiR-RNO-114608. Platelet degranulation.
R-RNO-1257604. PIP3 activates AKT signaling.
R-RNO-186763. Downstream signal transduction.
R-RNO-186797. Signaling by PDGF.
R-RNO-3000171. Non-integrin membrane-ECM interactions.
R-RNO-5673001. RAF/MAP kinase cascade.
R-RNO-6811558. PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling.

Miscellaneous databases

PROiP28576.

Gene expression databases

BgeeiENSRNOG00000001312.
ExpressionAtlasiP28576. baseline and differential.
GenevisibleiP28576. RN.

Family and domain databases

Gene3Di2.10.90.10. 1 hit.
InterProiIPR029034. Cystine-knot_cytokine.
IPR023581. PD_growth_factor_CS.
IPR000072. PDGF/VEGF_dom.
IPR006782. PDGF_N.
[Graphical view]
PfamiPF00341. PDGF. 1 hit.
PF04692. PDGF_N. 1 hit.
[Graphical view]
SMARTiSM00141. PDGF. 1 hit.
[Graphical view]
SUPFAMiSSF57501. SSF57501. 1 hit.
PROSITEiPS00249. PDGF_1. 1 hit.
PS50278. PDGF_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPDGFA_RAT
AccessioniPrimary (citable) accession number: P28576
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: February 1, 1994
Last modified: September 7, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.