Reviewed,
UniProtKB/Swiss-Prot P28572 (SC6A9_RAT)
Last modified
June 16, 2009.
Version 76.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
Customize display | text xml rdf/xml gff fasta |
Names and origin
| Protein names | Recommended name: Sodium- and chloride-dependent glycine transporter 1 Short name=GlyT1 Short name=GlyT-1 Alternative name(s): Solute carrier family 6 member 9 | ||
| Gene names |
| ||
| Organism | Rattus norvegicus (Rat) | ||
| Taxonomic identifier | 10116 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 633 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Terminates the action of glycine by its high affinity sodium-dependent reuptake into presynaptic terminals. May play a role in regulation of glycine levels in NMDA receptor-mediated neurotransmission. |
| Subcellular location | |
| Tissue specificity | GLYT-1A is found in the gray matter of CNS as well as in macrophages and mast cells in peripherical tissues. GLYT-1B is only found in the white matter of CNS. |
| Sequence similarities | Belongs to the sodium:neurotransmitter symporter (SNF) family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Amino-acid transport Neurotransmitter transport Symport Transport |
| Cellular component | Membrane |
| Coding sequence diversity | Alternative splicing |
| Domain | Transmembrane |
| PTM | Glycoprotein |
| Gene Ontology (GO) | |
| Biological process | amino acid transport Inferred from electronic annotation. Source: UniProtKB-KW neurotransmitter transportInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | integral to plasma membrane Inferred from electronic annotation. Source: InterPro |
| Molecular function | glycine transmembrane transporter activity Ref.2 Traceable author statement. Source: RGD neurotransmitter:sodium symporter activityInferred from electronic annotation. Source: InterPro protein bindingInferred from physical interaction. Source: IntAct sodium:amino acid symporter activityInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| Dlg4 | P31016 | 2 | EBI-848783,EBI-375655 | |
| Dlg4 | P31016 | 2 | EBI-848796,EBI-375655 | |
| Itga1 | P18614 | 1 | EBI-848783,EBI-848806 |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: P28572-1) Also known as: GlyT-1A; GlyT-2; This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: P28572-2) Also known as: GlyT-1B; GlyT-1; The sequence of this isoform differs from the canonical sequence as follows: 1-10: MVGKGAKGML → MAVAHGPVATSSPEQ |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 633 | 633 | Sodium- and chloride-dependent glycine transporter 1 | PRO_0000214782 | |||||
Regions | |||||||||
| Topological domain | 1 – 35 | 35 | Cytoplasmic Potential | ||||||
| Transmembrane | 36 – 56 | 21 | 1 Potential | ||||||
| Transmembrane | 64 – 83 | 20 | 2 Potential | ||||||
| Transmembrane | 107 – 127 | 21 | 3 Potential | ||||||
| Topological domain | 128 – 214 | 87 | Extracellular Potential | ||||||
| Transmembrane | 215 – 233 | 19 | 4 Potential | ||||||
| Transmembrane | 242 – 259 | 18 | 5 Potential | ||||||
| Transmembrane | 295 – 312 | 18 | 6 Potential | ||||||
| Transmembrane | 324 – 345 | 22 | 7 Potential | ||||||
| Transmembrane | 378 – 397 | 20 | 8 Potential | ||||||
| Transmembrane | 426 – 444 | 19 | 9 Potential | ||||||
| Transmembrane | 460 – 480 | 21 | 10 Potential | ||||||
| Transmembrane | 501 – 520 | 20 | 11 Potential | ||||||
| Transmembrane | 539 – 557 | 19 | 12 Potential | ||||||
| Topological domain | 558 – 633 | 76 | Cytoplasmic Potential | ||||||
Amino acid modifications | |||||||||
| Glycosylation | 164 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 167 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 177 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 183 | 1 | N-linked (GlcNAc...) Potential | ||||||
Natural variations | |||||||||
| Alternative sequence | 1 – 10 | 10 | MVGKGAKGML → MAVAHGPVATSSPEQ in isoform 2. | VSP_006273 | |||||
Sequences
| ||||||||||||||||||||||||
References
| « Hide 'large scale' references | |
| [1] | "Cloning, expression, and localization of a rat brain high-affinity glycine transporter." Guastella J., Brecha N., Weigmann C., Lester H.A., Davidson N. Proc. Natl. Acad. Sci. U.S.A. 89:7189-7193(1992) [PubMed: 1353889] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: Sprague-Dawley. Tissue: Brain. |
| [2] | "Cloning and expression of a glycine transporter reveal colocalization with NMDA receptors." Smith K.E., Borden L.A., Hartig P.R., Branchek T.A., Weinshank R.L. Neuron 8:927-935(1992) [PubMed: 1534013] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Brain. |
| [3] | "Two glycine transporter variants with distinct localization in the CNS and peripheral tissues are encoded by a common gene." Borowsky B., Mezey E., Hoffman B.J. Neuron 10:851-863(1993) [PubMed: 8494645] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Blood. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain. |
Cross-references
Sequence databases | |
|---|---|
| M95413 mRNA. Translation: AAA41256.1. M88595 mRNA. Translation: AAA41257.1. L13600 mRNA. Translation: AAA73557.1. BC128732 mRNA. Translation: AAI28733.1. | |
| IPI | IPI00201849. IPI00231844. |
| PIR | I58140. JH0673. |
| RefSeq | NP_446270.1. |
| UniGene | Rn.32110 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P28572. 3 interactions. |
Protein family/group databases | |
| TCDB | 2.A.22.2.2. neurotransmitter:sodium symporter (NSS) family. |
Genome annotation databases | |
| Ensembl | ENSRNOG00000019484. Rattus norvegicus. [Contig view] |
| GeneID | 116509. |
| KEGG | rno:116509. |
Organism-specific databases | |
| RGD | 621243. Slc6a9. |
Phylogenomic databases | |
| HOVERGEN | P28572. |
Gene expression databases | |
| ArrayExpress | P28572. |
| GermOnline | ENSRNOG00000019484. Rattus norvegicus. |
Family and domain databases | |
| InterPro | IPR000175. Na/ntran_symport. IPR003028. Na/ntran_symport_glycine_GLY1. [Graphical view] |
| PANTHER | PTHR11616. Na/ntran_symport. 1 hit. |
| Pfam | PF00209. SNF. 1 hit. [Graphical view] |
| PRINTS | PR01204. GLY1TRNSPORT. PR00176. NANEUSMPORT. |
| ProDom | PD000448. Na/ntran_symport. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| PROSITE | PS00610. NA_NEUROTRAN_SYMP_1. 1 hit. PS00754. NA_NEUROTRAN_SYMP_2. 1 hit. PS50267. NA_NEUROTRAN_SYMP_3. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 619129. |
Entry information
| Entry name | SC6A9_RAT | ||||||||
| Accession | Primary (citable) accession number: P28572 Secondary accession number(s): A1A5N0 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||

Clusters with


