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Reviewed, UniProtKB/Swiss-Prot P28572 (SC6A9_RAT)

Last modified June 16, 2009. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Sodium- and chloride-dependent glycine transporter 1
      Short name=GlyT1
      Short name=GlyT-1
Alternative name(s):
    Solute carrier family 6 member 9
Gene names
Name: Slc6a9
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length633 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Terminates the action of glycine by its high affinity sodium-dependent reuptake into presynaptic terminals. May play a role in regulation of glycine levels in NMDA receptor-mediated neurotransmission.

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

GLYT-1A is found in the gray matter of CNS as well as in macrophages and mast cells in peripherical tissues. GLYT-1B is only found in the white matter of CNS.

Sequence similarities

Belongs to the sodium:neurotransmitter symporter (SNF) family.

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

Dlg4P310162EBI-848783,EBI-375655
Dlg4P310162EBI-848796,EBI-375655
Itga1P186141EBI-848783,EBI-848806

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P28572-1)

Also known as: GlyT-1A; GlyT-2;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P28572-2)

Also known as: GlyT-1B; GlyT-1;

The sequence of this isoform differs from the canonical sequence as follows:
     1-10: MVGKGAKGML → MAVAHGPVATSSPEQ

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 633633Sodium- and chloride-dependent glycine transporter 1
PRO_0000214782

Regions

Topological domain1 – 3535Cytoplasmic Potential
Transmembrane36 – 56211 Potential
Transmembrane64 – 83202 Potential
Transmembrane107 – 127213 Potential
Topological domain128 – 21487Extracellular Potential
Transmembrane215 – 233194 Potential
Transmembrane242 – 259185 Potential
Transmembrane295 – 312186 Potential
Transmembrane324 – 345227 Potential
Transmembrane378 – 397208 Potential
Transmembrane426 – 444199 Potential
Transmembrane460 – 4802110 Potential
Transmembrane501 – 5202011 Potential
Transmembrane539 – 5571912 Potential
Topological domain558 – 63376Cytoplasmic Potential

Amino acid modifications

Glycosylation1641N-linked (GlcNAc...) Potential
Glycosylation1671N-linked (GlcNAc...) Potential
Glycosylation1771N-linked (GlcNAc...) Potential
Glycosylation1831N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1 – 1010MVGKGAKGML → MAVAHGPVATSSPEQ in isoform 2.
VSP_006273

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Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (GlyT-1A) (GlyT-2) [UniParc].

Last modified December 1, 1992. Version 1.
Checksum: 7D25B555B97E9E17

FASTA63370,571
        10         20         30         40         50         60 
MVGKGAKGML NGAVPSEATK KDQNLTRGNW GNQIEFVLTS VGYAVGLGNV WRFPYLCYRN 

        70         80         90        100        110        120 
GGGAFMFPYF IMLVFCGIPL FFMELSFGQF ASQGCLGVWR ISPMFKGVGY GMMVVSTYIG 

       130        140        150        160        170        180 
IYYNVVICIA FYYFFSSMTH VLPWAYCNNP WNTPDCAGVL DASNLTNGSR PTALSGNLSH 

       190        200        210        220        230        240 
LFNYTLQRTS PSEEYWRLYV LKLSDDIGDF GEVRLPLLGC LGVSWVVVFL CLIRGVKSSG 

       250        260        270        280        290        300 
KVVYFTATFP YVVLTILFVR GVTLEGAFTG IMYYLTPKWD KILEAKVWGD AASQIFYSLG 

       310        320        330        340        350        360 
CAWGGLITMA SYNKFHNNCY RDSVIISITN CATSVYAGFV IFSILGFMAN HLGVDVSRVA 

       370        380        390        400        410        420 
DHGPGLAFVA YPEALTLLPI SPLWSLLFFF MLILLGLGTQ FCLLETLVTA IVDEVGNEWI 

       430        440        450        460        470        480 
LQKKTYVTLG VAVAGFLLGI PLTSQAGIYW LLLMDNYAAS FSLVVISCIM CVSIMYIYGH 

       490        500        510        520        530        540 
RNYFQDIQMM LGFPPPLFFQ ICWRFVSPTI IFFILIFTVI QYRPITYNHY QYPGWAVAIG 

       550        560        570        580        590        600 
FLMALSSVIC IPLYALFQLC RTDGDTLLQR LKNATKPSRD WGPALLEHRT GRYAPTTTPS 

       610        620        630 
PEDGFEVQPL HPDKAQIPIV GSNGSSRLQD SRI

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Isoform 2 (GlyT-1B) (GlyT-1).

Checksum: AB43FCDE767F4AFA
Show »

FASTA63871,061

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References

« Hide 'large scale' references
[1]"Cloning, expression, and localization of a rat brain high-affinity glycine transporter."
Guastella J., Brecha N., Weigmann C., Lester H.A., Davidson N.
Proc. Natl. Acad. Sci. U.S.A. 89:7189-7193(1992) [PubMed: 1353889] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: Sprague-Dawley.
Tissue: Brain.
[2]"Cloning and expression of a glycine transporter reveal colocalization with NMDA receptors."
Smith K.E., Borden L.A., Hartig P.R., Branchek T.A., Weinshank R.L.
Neuron 8:927-935(1992) [PubMed: 1534013] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Tissue: Brain.
[3]"Two glycine transporter variants with distinct localization in the CNS and peripheral tissues are encoded by a common gene."
Borowsky B., Mezey E., Hoffman B.J.
Neuron 10:851-863(1993) [PubMed: 8494645] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Blood.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.

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Cross-references

Sequence databases

M95413 mRNA. Translation: AAA41256.1.
M88595 mRNA. Translation: AAA41257.1.
L13600 mRNA. Translation: AAA73557.1.
BC128732 mRNA. Translation: AAI28733.1.
IPIIPI00201849.
IPI00231844.
PIRI58140.
JH0673.
RefSeqNP_446270.1.
UniGeneRn.32110

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

IntActP28572. 3 interactions.

Protein family/group databases

TCDB2.A.22.2.2. neurotransmitter:sodium symporter (NSS) family.

Genome annotation databases

EnsemblENSRNOG00000019484. Rattus norvegicus. [Contig view]
GeneID116509.
KEGGrno:116509.

Organism-specific databases

RGD621243. Slc6a9.

Phylogenomic databases

HOVERGENP28572.

Gene expression databases

ArrayExpressP28572.
GermOnlineENSRNOG00000019484. Rattus norvegicus.

Family and domain databases

InterProIPR000175. Na/ntran_symport.
IPR003028. Na/ntran_symport_glycine_GLY1.
[Graphical view]
PANTHERPTHR11616. Na/ntran_symport. 1 hit.
PfamPF00209. SNF. 1 hit.
[Graphical view]
PRINTSPR01204. GLY1TRNSPORT.
PR00176. NANEUSMPORT.
ProDomPD000448. Na/ntran_symport. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio619129.

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Entry information

Entry nameSC6A9_RAT
AccessionPrimary (citable) accession number: P28572
Secondary accession number(s): A1A5N0
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: June 16, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents