Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P28572 (SC6A9_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sodium- and chloride-dependent glycine transporter 1

Short name=GlyT-1
Short name=GlyT1
Alternative name(s):
Solute carrier family 6 member 9
Gene names
Name:Slc6a9
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length638 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Terminates the action of glycine by its high affinity sodium-dependent reuptake into presynaptic terminals. May play a role in regulation of glycine levels in NMDA receptor-mediated neurotransmission. Ref.2

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Isoform 1 is only found in the white matter of the CNS. Isoform 2 is found in the gray matter of CNS as well as in macrophages and mast cells in peripherical tissues. Ref.1 Ref.2 Ref.3

Sequence similarities

Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A9 subfamily. [View classification]

Biophysicochemical properties

Kinetic parameters:

KM=123 µM for glycine Ref.2

Vmax=28 nmol/min/mg enzyme with glycine as substrate

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Dlg4P310164EBI-848796,EBI-375655

Alternative products

This entry describes 2 isoforms produced by alternative promoter usage. [Align] [Select]
Isoform 1 (identifier: P28572-2)

Also known as: GlyT-1B; GlyT-1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P28572-1)

Also known as: GlyT-1A; GlyT-2;

The sequence of this isoform differs from the canonical sequence as follows:
     1-15: MAVAHGPVATSSPEQ → MVGKGAKGML

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 638638Sodium- and chloride-dependent glycine transporter 1
PRO_0000214782

Regions

Topological domain1 – 4040Cytoplasmic Potential
Transmembrane41 – 6121Helical; Name=1; Potential
Transmembrane68 – 8821Helical; Name=2; Potential
Transmembrane120 – 14021Helical; Name=3; Potential
Topological domain141 – 21777Extracellular Potential
Transmembrane218 – 23821Helical; Name=4; Potential
Transmembrane247 – 26721Helical; Name=5; Potential
Transmembrane292 – 31221Helical; Name=6; Potential
Transmembrane339 – 35921Helical; Name=7; Potential
Transmembrane382 – 40221Helical; Name=8; Potential
Transmembrane438 – 45821Helical; Name=9; Potential
Transmembrane462 – 48221Helical; Name=10; Potential
Transmembrane502 – 52221Helical; Name=11; Potential
Transmembrane542 – 56221Helical; Name=12; Potential
Topological domain563 – 63876Cytoplasmic Potential

Amino acid modifications

Glycosylation1691N-linked (GlcNAc...) Potential
Glycosylation1721N-linked (GlcNAc...) Potential
Glycosylation1821N-linked (GlcNAc...) Potential
Glycosylation1881N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1 – 1515MAVAH…SSPEQ → MVGKGAKGML in isoform 2.
VSP_039239

Experimental info

Sequence conflict501V → W in AAC71066. Ref.5
Sequence conflict501V → W in AAC71067. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (GlyT-1B) (GlyT-1) [UniParc].

Last modified May 18, 2010. Version 2.
Checksum: AB43FCDE767F4AFA

FASTA63871,061
        10         20         30         40         50         60 
MAVAHGPVAT SSPEQNGAVP SEATKKDQNL TRGNWGNQIE FVLTSVGYAV GLGNVWRFPY 

        70         80         90        100        110        120 
LCYRNGGGAF MFPYFIMLVF CGIPLFFMEL SFGQFASQGC LGVWRISPMF KGVGYGMMVV 

       130        140        150        160        170        180 
STYIGIYYNV VICIAFYYFF SSMTHVLPWA YCNNPWNTPD CAGVLDASNL TNGSRPTALS 

       190        200        210        220        230        240 
GNLSHLFNYT LQRTSPSEEY WRLYVLKLSD DIGDFGEVRL PLLGCLGVSW VVVFLCLIRG 

       250        260        270        280        290        300 
VKSSGKVVYF TATFPYVVLT ILFVRGVTLE GAFTGIMYYL TPKWDKILEA KVWGDAASQI 

       310        320        330        340        350        360 
FYSLGCAWGG LITMASYNKF HNNCYRDSVI ISITNCATSV YAGFVIFSIL GFMANHLGVD 

       370        380        390        400        410        420 
VSRVADHGPG LAFVAYPEAL TLLPISPLWS LLFFFMLILL GLGTQFCLLE TLVTAIVDEV 

       430        440        450        460        470        480 
GNEWILQKKT YVTLGVAVAG FLLGIPLTSQ AGIYWLLLMD NYAASFSLVV ISCIMCVSIM 

       490        500        510        520        530        540 
YIYGHRNYFQ DIQMMLGFPP PLFFQICWRF VSPTIIFFIL IFTVIQYRPI TYNHYQYPGW 

       550        560        570        580        590        600 
AVAIGFLMAL SSVICIPLYA LFQLCRTDGD TLLQRLKNAT KPSRDWGPAL LEHRTGRYAP 

       610        620        630 
TTTPSPEDGF EVQPLHPDKA QIPIVGSNGS SRLQDSRI 

« Hide

Isoform 2 (GlyT-1A) (GlyT-2) [UniParc].

Checksum: 7D25B555B97E9E17
Show »

FASTA63370,571

References

« Hide 'large scale' references
[1]"Cloning, expression, and localization of a rat brain high-affinity glycine transporter."
Guastella J., Brecha N., Weigmann C., Lester H.A., Davidson N.
Proc. Natl. Acad. Sci. U.S.A. 89:7189-7193(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY.
Strain: Sprague-Dawley.
Tissue: Brain.
[2]"Cloning and expression of a glycine transporter reveal colocalization with NMDA receptors."
Smith K.E., Borden L.A., Hartig P.R., Branchek T.A., Weinshank R.L.
Neuron 8:927-935(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
Tissue: Brain.
[3]"Two glycine transporter variants with distinct localization in the CNS and peripheral tissues are encoded by a common gene."
Borowsky B., Mezey E., Hoffman B.J.
Neuron 10:851-863(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY.
Tissue: Blood.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Brain.
[5]"Analysis of a gene encoding two glycine transporter variants reveals alternative promoter usage and a novel gene structure."
Borowsky B., Hoffman B.J.
J. Biol. Chem. 273:29077-29085(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-67 (ISOFORMS 1 AND 2), ALTERNATIVE PROMOTER USAGE.
Strain: Sprague-Dawley.
Tissue: Brain.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M95413 mRNA. Translation: AAA41256.1.
M88595 mRNA. Translation: AAA41257.1.
L13600 mRNA. Translation: AAA73557.1.
BC128732 mRNA. Translation: AAI28733.1.
U28975 Genomic DNA. Translation: AAC71066.1.
U28975 Genomic DNA. Translation: AAC71067.1.
PIRI58140.
JH0673.
RefSeqNP_446270.1. NM_053818.2. [P28572-1]
XP_006238687.1. XM_006238625.1. [P28572-2]
UniGeneRn.32110.

3D structure databases

ProteinModelPortalP28572.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid250476. 1 interaction.
IntActP28572. 2 interactions.
STRING10116.ENSRNOP00000026348.

Chemistry

BindingDBP28572.
ChEMBLCHEMBL4556.

Protein family/group databases

TCDB2.A.22.2.2. the neurotransmitter:sodium symporter (nss) family.

Proteomic databases

PRIDEP28572.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000048770; ENSRNOP00000040252; ENSRNOG00000019484. [P28572-1]
GeneID116509.
KEGGrno:116509.

Organism-specific databases

CTD6536.
RGD621243. Slc6a9.

Phylogenomic databases

eggNOGCOG0733.
GeneTreeENSGT00740000115427.
HOGENOMHOG000116406.
HOVERGENHBG071421.
InParanoidQ63323.
KOK05038.
OMAFQLCRTD.
OrthoDBEOG793B71.
PhylomeDBP28572.
TreeFamTF343812.

Gene expression databases

GenevestigatorP28572.

Family and domain databases

InterProIPR000175. Na/ntran_symport.
IPR003028. Na/ntran_symport_glycine_GLY1.
[Graphical view]
PANTHERPTHR11616. PTHR11616. 1 hit.
PfamPF00209. SNF. 1 hit.
[Graphical view]
PRINTSPR01204. GLY1TRNSPORT.
PR00176. NANEUSMPORT.
PROSITEPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio619129.
PMAP-CutDBQ63322.
PROP28572.

Entry information

Entry nameSC6A9_RAT
AccessionPrimary (citable) accession number: P28572
Secondary accession number(s): A1A5N0, Q63322, Q63323
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: May 18, 2010
Last modified: June 11, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families