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P28572

- SC6A9_RAT

UniProt

P28572 - SC6A9_RAT

Protein

Sodium- and chloride-dependent glycine transporter 1

Gene

Slc6a9

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 2 (18 May 2010)
      Previous versions | rss
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    Functioni

    Terminates the action of glycine by its high affinity sodium-dependent reuptake into presynaptic terminals. May play a role in regulation of glycine levels in NMDA receptor-mediated neurotransmission.1 Publication

    Kineticsi

    1. KM=123 µM for glycine1 Publication

    Vmax=28 nmol/min/mg enzyme with glycine as substrate1 Publication

    GO - Molecular functioni

    1. glycine transmembrane transporter activity Source: RGD
    2. neurotransmitter:sodium symporter activity Source: InterPro
    3. protein binding Source: IntAct
    4. sodium:amino acid symporter activity Source: InterPro

    Keywords - Biological processi

    Amino-acid transport, Neurotransmitter transport, Symport, Transport

    Protein family/group databases

    TCDBi2.A.22.2.2. the neurotransmitter:sodium symporter (nss) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium- and chloride-dependent glycine transporter 1
    Short name:
    GlyT-1
    Short name:
    GlyT1
    Alternative name(s):
    Solute carrier family 6 member 9
    Gene namesi
    Name:Slc6a9
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 5

    Organism-specific databases

    RGDi621243. Slc6a9.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of plasma membrane Source: InterPro

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 638638Sodium- and chloride-dependent glycine transporter 1PRO_0000214782Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi169 – 1691N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi172 – 1721N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi182 – 1821N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi188 – 1881N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiP28572.

    Miscellaneous databases

    PMAP-CutDBQ63322.

    Expressioni

    Tissue specificityi

    Isoform 1 is only found in the white matter of the CNS. Isoform 2 is found in the gray matter of CNS as well as in macrophages and mast cells in peripherical tissues.3 Publications

    Gene expression databases

    GenevestigatoriP28572.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Dlg4P310164EBI-848796,EBI-375655

    Protein-protein interaction databases

    BioGridi250476. 1 interaction.
    IntActiP28572. 2 interactions.
    STRINGi10116.ENSRNOP00000026348.

    Structurei

    3D structure databases

    ProteinModelPortaliP28572.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 4040CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini141 – 21777ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini563 – 63876CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei41 – 6121Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei68 – 8821Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei120 – 14021Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei218 – 23821Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei247 – 26721Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei292 – 31221Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei339 – 35921Helical; Name=7Sequence AnalysisAdd
    BLAST
    Transmembranei382 – 40221Helical; Name=8Sequence AnalysisAdd
    BLAST
    Transmembranei438 – 45821Helical; Name=9Sequence AnalysisAdd
    BLAST
    Transmembranei462 – 48221Helical; Name=10Sequence AnalysisAdd
    BLAST
    Transmembranei502 – 52221Helical; Name=11Sequence AnalysisAdd
    BLAST
    Transmembranei542 – 56221Helical; Name=12Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0733.
    GeneTreeiENSGT00740000115427.
    HOGENOMiHOG000116406.
    HOVERGENiHBG071421.
    InParanoidiQ63323.
    KOiK05038.
    OMAiFQLCRTD.
    OrthoDBiEOG793B71.
    PhylomeDBiP28572.
    TreeFamiTF343812.

    Family and domain databases

    InterProiIPR000175. Na/ntran_symport.
    IPR003028. Na/ntran_symport_glycine_GLY1.
    [Graphical view]
    PANTHERiPTHR11616. PTHR11616. 1 hit.
    PfamiPF00209. SNF. 1 hit.
    [Graphical view]
    PRINTSiPR01204. GLY1TRNSPORT.
    PR00176. NANEUSMPORT.
    PROSITEiPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
    PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
    PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative promoter usage. Align

    Isoform 1 (identifier: P28572-2) [UniParc]FASTAAdd to Basket

    Also known as: GlyT-1B, GlyT-1

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAVAHGPVAT SSPEQNGAVP SEATKKDQNL TRGNWGNQIE FVLTSVGYAV    50
    GLGNVWRFPY LCYRNGGGAF MFPYFIMLVF CGIPLFFMEL SFGQFASQGC 100
    LGVWRISPMF KGVGYGMMVV STYIGIYYNV VICIAFYYFF SSMTHVLPWA 150
    YCNNPWNTPD CAGVLDASNL TNGSRPTALS GNLSHLFNYT LQRTSPSEEY 200
    WRLYVLKLSD DIGDFGEVRL PLLGCLGVSW VVVFLCLIRG VKSSGKVVYF 250
    TATFPYVVLT ILFVRGVTLE GAFTGIMYYL TPKWDKILEA KVWGDAASQI 300
    FYSLGCAWGG LITMASYNKF HNNCYRDSVI ISITNCATSV YAGFVIFSIL 350
    GFMANHLGVD VSRVADHGPG LAFVAYPEAL TLLPISPLWS LLFFFMLILL 400
    GLGTQFCLLE TLVTAIVDEV GNEWILQKKT YVTLGVAVAG FLLGIPLTSQ 450
    AGIYWLLLMD NYAASFSLVV ISCIMCVSIM YIYGHRNYFQ DIQMMLGFPP 500
    PLFFQICWRF VSPTIIFFIL IFTVIQYRPI TYNHYQYPGW AVAIGFLMAL 550
    SSVICIPLYA LFQLCRTDGD TLLQRLKNAT KPSRDWGPAL LEHRTGRYAP 600
    TTTPSPEDGF EVQPLHPDKA QIPIVGSNGS SRLQDSRI 638
    Length:638
    Mass (Da):71,061
    Last modified:May 18, 2010 - v2
    Checksum:iAB43FCDE767F4AFA
    GO
    Isoform 2 (identifier: P28572-1) [UniParc]FASTAAdd to Basket

    Also known as: GlyT-1A, GlyT-2

    The sequence of this isoform differs from the canonical sequence as follows:
         1-15: MAVAHGPVATSSPEQ → MVGKGAKGML

    Show »
    Length:633
    Mass (Da):70,571
    Checksum:i7D25B555B97E9E17
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti50 – 501V → W in AAC71066. (PubMed:9786914)Curated
    Sequence conflicti50 – 501V → W in AAC71067. (PubMed:9786914)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 1515MAVAH…SSPEQ → MVGKGAKGML in isoform 2. 3 PublicationsVSP_039239Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M95413 mRNA. Translation: AAA41256.1.
    M88595 mRNA. Translation: AAA41257.1.
    L13600 mRNA. Translation: AAA73557.1.
    BC128732 mRNA. Translation: AAI28733.1.
    U28975 Genomic DNA. Translation: AAC71066.1.
    U28975 Genomic DNA. Translation: AAC71067.1.
    PIRiI58140.
    JH0673.
    RefSeqiNP_446270.1. NM_053818.2. [P28572-1]
    XP_006238687.1. XM_006238625.1. [P28572-2]
    UniGeneiRn.32110.

    Genome annotation databases

    EnsembliENSRNOT00000048770; ENSRNOP00000040252; ENSRNOG00000019484. [P28572-1]
    GeneIDi116509.
    KEGGirno:116509.

    Keywords - Coding sequence diversityi

    Alternative promoter usage

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M95413 mRNA. Translation: AAA41256.1 .
    M88595 mRNA. Translation: AAA41257.1 .
    L13600 mRNA. Translation: AAA73557.1 .
    BC128732 mRNA. Translation: AAI28733.1 .
    U28975 Genomic DNA. Translation: AAC71066.1 .
    U28975 Genomic DNA. Translation: AAC71067.1 .
    PIRi I58140.
    JH0673.
    RefSeqi NP_446270.1. NM_053818.2. [P28572-1 ]
    XP_006238687.1. XM_006238625.1. [P28572-2 ]
    UniGenei Rn.32110.

    3D structure databases

    ProteinModelPortali P28572.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 250476. 1 interaction.
    IntActi P28572. 2 interactions.
    STRINGi 10116.ENSRNOP00000026348.

    Chemistry

    BindingDBi P28572.
    ChEMBLi CHEMBL4556.

    Protein family/group databases

    TCDBi 2.A.22.2.2. the neurotransmitter:sodium symporter (nss) family.

    Proteomic databases

    PRIDEi P28572.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000048770 ; ENSRNOP00000040252 ; ENSRNOG00000019484 . [P28572-1 ]
    GeneIDi 116509.
    KEGGi rno:116509.

    Organism-specific databases

    CTDi 6536.
    RGDi 621243. Slc6a9.

    Phylogenomic databases

    eggNOGi COG0733.
    GeneTreei ENSGT00740000115427.
    HOGENOMi HOG000116406.
    HOVERGENi HBG071421.
    InParanoidi Q63323.
    KOi K05038.
    OMAi FQLCRTD.
    OrthoDBi EOG793B71.
    PhylomeDBi P28572.
    TreeFami TF343812.

    Miscellaneous databases

    NextBioi 619129.
    PMAP-CutDB Q63322.
    PROi P28572.

    Gene expression databases

    Genevestigatori P28572.

    Family and domain databases

    InterProi IPR000175. Na/ntran_symport.
    IPR003028. Na/ntran_symport_glycine_GLY1.
    [Graphical view ]
    PANTHERi PTHR11616. PTHR11616. 1 hit.
    Pfami PF00209. SNF. 1 hit.
    [Graphical view ]
    PRINTSi PR01204. GLY1TRNSPORT.
    PR00176. NANEUSMPORT.
    PROSITEi PS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
    PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
    PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, expression, and localization of a rat brain high-affinity glycine transporter."
      Guastella J., Brecha N., Weigmann C., Lester H.A., Davidson N.
      Proc. Natl. Acad. Sci. U.S.A. 89:7189-7193(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY.
      Strain: Sprague-Dawley.
      Tissue: Brain.
    2. "Cloning and expression of a glycine transporter reveal colocalization with NMDA receptors."
      Smith K.E., Borden L.A., Hartig P.R., Branchek T.A., Weinshank R.L.
      Neuron 8:927-935(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
      Tissue: Brain.
    3. "Two glycine transporter variants with distinct localization in the CNS and peripheral tissues are encoded by a common gene."
      Borowsky B., Mezey E., Hoffman B.J.
      Neuron 10:851-863(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY.
      Tissue: Blood.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Brain.
    5. "Analysis of a gene encoding two glycine transporter variants reveals alternative promoter usage and a novel gene structure."
      Borowsky B., Hoffman B.J.
      J. Biol. Chem. 273:29077-29085(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-67 (ISOFORMS 1 AND 2), ALTERNATIVE PROMOTER USAGE.
      Strain: Sprague-Dawley.
      Tissue: Brain.

    Entry informationi

    Entry nameiSC6A9_RAT
    AccessioniPrimary (citable) accession number: P28572
    Secondary accession number(s): A1A5N0, Q63322, Q63323
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: May 18, 2010
    Last modified: October 1, 2014
    This is version 118 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3