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Protein

Sodium- and chloride-dependent glycine transporter 1

Gene

Slc6a9

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Terminates the action of glycine by its high affinity sodium-dependent reuptake into presynaptic terminals. May play a role in regulation of glycine levels in NMDA receptor-mediated neurotransmission.

GO - Molecular functioni

GO - Biological processi

  • glycine import Source: GO_Central
  • glycine secretion, neurotransmission Source: MGI
  • glycine transport Source: MGI
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Neurotransmitter transport, Symport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-442660. Na+/Cl- dependent neurotransmitter transporters.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium- and chloride-dependent glycine transporter 1
Short name:
GlyT-1
Short name:
GlyT1
Alternative name(s):
Solute carrier family 6 member 9
Gene namesi
Name:Slc6a9
Synonyms:Glyt1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:95760. Slc6a9.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 94CytoplasmicSequence analysisAdd BLAST94
Transmembranei95 – 115Helical; Name=1Sequence analysisAdd BLAST21
Transmembranei122 – 142Helical; Name=2Sequence analysisAdd BLAST21
Transmembranei174 – 194Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini195 – 271ExtracellularSequence analysisAdd BLAST77
Transmembranei272 – 292Helical; Name=4Sequence analysisAdd BLAST21
Transmembranei301 – 321Helical; Name=5Sequence analysisAdd BLAST21
Transmembranei346 – 366Helical; Name=6Sequence analysisAdd BLAST21
Transmembranei393 – 413Helical; Name=7Sequence analysisAdd BLAST21
Transmembranei436 – 456Helical; Name=8Sequence analysisAdd BLAST21
Transmembranei492 – 512Helical; Name=9Sequence analysisAdd BLAST21
Transmembranei516 – 536Helical; Name=10Sequence analysisAdd BLAST21
Transmembranei556 – 576Helical; Name=11Sequence analysisAdd BLAST21
Transmembranei596 – 616Helical; Name=12Sequence analysisAdd BLAST21
Topological domaini617 – 692CytoplasmicSequence analysisAdd BLAST76

GO - Cellular componenti

  • integral component of membrane Source: MGI
  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: MGI
  • presynapse Source: GOC
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1075303.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002147811 – 692Sodium- and chloride-dependent glycine transporter 1Add BLAST692

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei657PhosphothreonineCombined sources1
Modified residuei659PhosphoserineCombined sources1
Modified residuei684PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP28571.
PRIDEiP28571.

PTM databases

iPTMnetiP28571.
PhosphoSitePlusiP28571.

Expressioni

Tissue specificityi

At E11, expressed in the ventral part of the ventricular zone. At E15, also expressed in adjacent mantle tissue and the meninges. Strongly expressed in E12 and E15 liver.2 Publications

Developmental stagei

Expression is present at low levels as early as E9 and E10, but strongly increases at E13 and remains at high levels up to E15. Also expressed in adult.1 Publication

Gene expression databases

BgeeiENSMUSG00000028542.
ExpressionAtlasiP28571. baseline and differential.
GenevisibleiP28571. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030269.

Chemistry databases

BindingDBiP28571.

Structurei

3D structure databases

ProteinModelPortaliP28571.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3659. Eukaryota.
COG0733. LUCA.
GeneTreeiENSGT00760000118857.
HOGENOMiHOG000116406.
HOVERGENiHBG071421.
InParanoidiP28571.
KOiK05038.
PhylomeDBiP28571.
TreeFamiTF343812.

Family and domain databases

InterProiIPR000175. Na/ntran_symport.
IPR003028. Na/ntran_symport_glycine_GLY1.
[Graphical view]
PANTHERiPTHR11616. PTHR11616. 2 hits.
PfamiPF00209. SNF. 1 hit.
[Graphical view]
PRINTSiPR01204. GLY1TRNSPORT.
PR00176. NANEUSMPORT.
PROSITEiPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative promoter usage and alternative splicing. AlignAdd to basket

Isoform GlyT-1C (identifier: P28571-3) [UniParc]FASTAAdd to basket
Also known as: GLYT1c

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIGGDTRAAS AHPGMASAQG PVATPSPEQP FPGTTSVSLA RPVLRVWHGA
60 70 80 90 100
HSSGLLPNLI AQHSPAMAQN GAVPSEATKK DQNLTRGNWG NQIEFVLTSV
110 120 130 140 150
GYAVGLGNVW RFPYLCYRNG GGAFMFPYFI MLIFCGIPLF FMELSFGQFA
160 170 180 190 200
SQGCLGVWRI SPMFKGVGYG MMVVSTYIGI YYNVVICIAF YYFFSSMTHV
210 220 230 240 250
LPWAYCNNPW NTPDCAGVLD ASNLTNGSRP AALSGNLSHL FNYTLQRTSP
260 270 280 290 300
SEEYWRLYVL KLSDDIGNFG EVRLPLLGCL GVSWVVVFLC LIRGVKSSGK
310 320 330 340 350
VVYFTATFPY VVLTILFVRG VTLEGAFTGI MYYLTPQWDK ILEAKVWGDA
360 370 380 390 400
ASQIFYSLGC AWGGLITMAS YNKFHNNCYR DSVIISITNC ATSVYAGFVI
410 420 430 440 450
FSILGFMANH LGVDVSRVAD HGPGLAFVAY PEALTLLPIS PLWSLLFFFM
460 470 480 490 500
LILLGLGTQF CLLETLVTAI VDEVGNEWIL QKKTYVTLGV AVAGFLLGIP
510 520 530 540 550
LTSQAGIYWL LLMDNYAASF SLVVISCIMC VSIMYIYGHR NYFQDIQMML
560 570 580 590 600
GFPPPLFFQI CWRFVSPAII FFILIFTVIQ YRPITYNHYQ YPGWAVAIGF
610 620 630 640 650
LMALSSVICI PLYALFQLCR TDGDTLLQRL KNATKPSRDW GPALLEHRTG
660 670 680 690
RYAPTTTPSP EDGFEVQPLH PDKAQIPIVG SNGSSRFQDS RI
Note: Produced by alternative promoter usage.
Length:692
Mass (Da):76,544
Last modified:August 10, 2010 - v3
Checksum:iC4A78A6562DAB01E
GO
Isoform GlyT-1A (identifier: P28571-1) [UniParc]FASTAAdd to basket
Also known as: GLYT1a

The sequence of this isoform differs from the canonical sequence as follows:
     1-69: MIGGDTRAAS...IAQHSPAMAQ → MVGKGAKGML

Note: Produced by alternative promoter usage.
Show »
Length:633
Mass (Da):70,558
Checksum:i51A0BD971F1BB794
GO
Isoform GlyT-1B (identifier: P28571-2) [UniParc]FASTAAdd to basket
Also known as: GLYT1b

The sequence of this isoform differs from the canonical sequence as follows:
     29-69: Missing.

Note: Produced by alternative splicing of isoform GlyT-1C.
Show »
Length:651
Mass (Da):72,231
Checksum:i47C32BDB76F52405
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti341I → Y in CAA47440 (PubMed:1618338).Curated1
Sequence conflicti341I → Y in X82570 (PubMed:7891186).Curated1
Sequence conflicti393 – 394SV → RL in CAA47440 (PubMed:1618338).Curated2
Sequence conflicti597A → R in CAA47440 (PubMed:1618338).Curated1
Sequence conflicti597A → R in X82571 (PubMed:7891186).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0062721 – 69MIGGD…PAMAQ → MVGKGAKGML in isoform GlyT-1A. 3 PublicationsAdd BLAST69
Alternative sequenceiVSP_03924129 – 69Missing in isoform GlyT-1B. 1 PublicationAdd BLAST41

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67056 mRNA. Translation: CAA47440.1.
X82567 Genomic DNA. No translation available.
X82568 Genomic DNA. No translation available.
X82569 Genomic DNA. No translation available.
X82570 Genomic DNA. No translation available.
X82571 Genomic DNA. No translation available.
X82572 Genomic DNA. No translation available.
AL627128 Genomic DNA. Translation: CAM14713.1.
BC021828 mRNA. Translation: AAH21828.1.
CCDSiCCDS18538.1. [P28571-1]
PIRiE46027.
S23151.
RefSeqiNP_032161.2. NM_008135.4. [P28571-1]
UniGeneiMm.244549.

Genome annotation databases

EnsembliENSMUST00000030269; ENSMUSP00000030269; ENSMUSG00000028542. [P28571-1]
ENSMUST00000063857; ENSMUSP00000066102; ENSMUSG00000028542. [P28571-1]
GeneIDi14664.
KEGGimmu:14664.
UCSCiuc008uiy.1. mouse. [P28571-1]

Keywords - Coding sequence diversityi

Alternative promoter usage, Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67056 mRNA. Translation: CAA47440.1.
X82567 Genomic DNA. No translation available.
X82568 Genomic DNA. No translation available.
X82569 Genomic DNA. No translation available.
X82570 Genomic DNA. No translation available.
X82571 Genomic DNA. No translation available.
X82572 Genomic DNA. No translation available.
AL627128 Genomic DNA. Translation: CAM14713.1.
BC021828 mRNA. Translation: AAH21828.1.
CCDSiCCDS18538.1. [P28571-1]
PIRiE46027.
S23151.
RefSeqiNP_032161.2. NM_008135.4. [P28571-1]
UniGeneiMm.244549.

3D structure databases

ProteinModelPortaliP28571.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030269.

Chemistry databases

BindingDBiP28571.
ChEMBLiCHEMBL1075303.

PTM databases

iPTMnetiP28571.
PhosphoSitePlusiP28571.

Proteomic databases

PaxDbiP28571.
PRIDEiP28571.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030269; ENSMUSP00000030269; ENSMUSG00000028542. [P28571-1]
ENSMUST00000063857; ENSMUSP00000066102; ENSMUSG00000028542. [P28571-1]
GeneIDi14664.
KEGGimmu:14664.
UCSCiuc008uiy.1. mouse. [P28571-1]

Organism-specific databases

CTDi6536.
MGIiMGI:95760. Slc6a9.

Phylogenomic databases

eggNOGiKOG3659. Eukaryota.
COG0733. LUCA.
GeneTreeiENSGT00760000118857.
HOGENOMiHOG000116406.
HOVERGENiHBG071421.
InParanoidiP28571.
KOiK05038.
PhylomeDBiP28571.
TreeFamiTF343812.

Enzyme and pathway databases

ReactomeiR-MMU-442660. Na+/Cl- dependent neurotransmitter transporters.

Miscellaneous databases

PROiP28571.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028542.
ExpressionAtlasiP28571. baseline and differential.
GenevisibleiP28571. MM.

Family and domain databases

InterProiIPR000175. Na/ntran_symport.
IPR003028. Na/ntran_symport_glycine_GLY1.
[Graphical view]
PANTHERiPTHR11616. PTHR11616. 2 hits.
PfamiPF00209. SNF. 1 hit.
[Graphical view]
PRINTSiPR01204. GLY1TRNSPORT.
PR00176. NANEUSMPORT.
PROSITEiPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSC6A9_MOUSE
AccessioniPrimary (citable) accession number: P28571
Secondary accession number(s): B1ASI9, Q8VC47
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: August 10, 2010
Last modified: November 2, 2016
This is version 132 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.