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Protein

Sodium- and chloride-dependent glycine transporter 1

Gene

Slc6a9

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Terminates the action of glycine by its high affinity sodium-dependent reuptake into presynaptic terminals. May play a role in regulation of glycine levels in NMDA receptor-mediated neurotransmission.

GO - Molecular functioni

GO - Biological processi

  • glycine transport Source: MGI
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Neurotransmitter transport, Symport, Transport

Enzyme and pathway databases

ReactomeiREACT_307956. Na+/Cl- dependent neurotransmitter transporters.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium- and chloride-dependent glycine transporter 1
Short name:
GlyT-1
Short name:
GlyT1
Alternative name(s):
Solute carrier family 6 member 9
Gene namesi
Name:Slc6a9
Synonyms:Glyt1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:95760. Slc6a9.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 9494CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei95 – 11521Helical; Name=1Sequence AnalysisAdd
BLAST
Transmembranei122 – 14221Helical; Name=2Sequence AnalysisAdd
BLAST
Transmembranei174 – 19421Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini195 – 27177ExtracellularSequence AnalysisAdd
BLAST
Transmembranei272 – 29221Helical; Name=4Sequence AnalysisAdd
BLAST
Transmembranei301 – 32121Helical; Name=5Sequence AnalysisAdd
BLAST
Transmembranei346 – 36621Helical; Name=6Sequence AnalysisAdd
BLAST
Transmembranei393 – 41321Helical; Name=7Sequence AnalysisAdd
BLAST
Transmembranei436 – 45621Helical; Name=8Sequence AnalysisAdd
BLAST
Transmembranei492 – 51221Helical; Name=9Sequence AnalysisAdd
BLAST
Transmembranei516 – 53621Helical; Name=10Sequence AnalysisAdd
BLAST
Transmembranei556 – 57621Helical; Name=11Sequence AnalysisAdd
BLAST
Transmembranei596 – 61621Helical; Name=12Sequence AnalysisAdd
BLAST
Topological domaini617 – 69276CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: MGI
  • integral component of plasma membrane Source: InterPro
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 692692Sodium- and chloride-dependent glycine transporter 1PRO_0000214781Add
BLAST

Proteomic databases

MaxQBiP28571.
PaxDbiP28571.
PRIDEiP28571.

PTM databases

PhosphoSiteiP28571.

Expressioni

Tissue specificityi

At E11, expressed in the ventral part of the ventricular zone. At E15, also expressed in adjacent mantle tissue and the meninges. Strongly expressed in E12 and E15 liver.2 Publications

Developmental stagei

Expression is present at low levels as early as E9 and E10, but strongly increases at E13 and remains at high levels up to E15. Also expressed in adult.1 Publication

Gene expression databases

BgeeiP28571.
ExpressionAtlasiP28571. baseline and differential.
GenevisibleiP28571. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030269.

Structurei

3D structure databases

ProteinModelPortaliP28571.
SMRiP28571. Positions 86-639.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0733.
GeneTreeiENSGT00760000118857.
HOGENOMiHOG000116406.
HOVERGENiHBG071421.
InParanoidiP28571.
KOiK05038.
OrthoDBiEOG793B71.
PhylomeDBiP28571.
TreeFamiTF343812.

Family and domain databases

InterProiIPR000175. Na/ntran_symport.
IPR003028. Na/ntran_symport_glycine_GLY1.
[Graphical view]
PANTHERiPTHR11616. PTHR11616. 1 hit.
PfamiPF00209. SNF. 1 hit.
[Graphical view]
PRINTSiPR01204. GLY1TRNSPORT.
PR00176. NANEUSMPORT.
PROSITEiPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative promoter usage and alternative splicing. AlignAdd to basket

Isoform GlyT-1C (identifier: P28571-3) [UniParc]FASTAAdd to basket

Also known as: GLYT1c

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIGGDTRAAS AHPGMASAQG PVATPSPEQP FPGTTSVSLA RPVLRVWHGA
60 70 80 90 100
HSSGLLPNLI AQHSPAMAQN GAVPSEATKK DQNLTRGNWG NQIEFVLTSV
110 120 130 140 150
GYAVGLGNVW RFPYLCYRNG GGAFMFPYFI MLIFCGIPLF FMELSFGQFA
160 170 180 190 200
SQGCLGVWRI SPMFKGVGYG MMVVSTYIGI YYNVVICIAF YYFFSSMTHV
210 220 230 240 250
LPWAYCNNPW NTPDCAGVLD ASNLTNGSRP AALSGNLSHL FNYTLQRTSP
260 270 280 290 300
SEEYWRLYVL KLSDDIGNFG EVRLPLLGCL GVSWVVVFLC LIRGVKSSGK
310 320 330 340 350
VVYFTATFPY VVLTILFVRG VTLEGAFTGI MYYLTPQWDK ILEAKVWGDA
360 370 380 390 400
ASQIFYSLGC AWGGLITMAS YNKFHNNCYR DSVIISITNC ATSVYAGFVI
410 420 430 440 450
FSILGFMANH LGVDVSRVAD HGPGLAFVAY PEALTLLPIS PLWSLLFFFM
460 470 480 490 500
LILLGLGTQF CLLETLVTAI VDEVGNEWIL QKKTYVTLGV AVAGFLLGIP
510 520 530 540 550
LTSQAGIYWL LLMDNYAASF SLVVISCIMC VSIMYIYGHR NYFQDIQMML
560 570 580 590 600
GFPPPLFFQI CWRFVSPAII FFILIFTVIQ YRPITYNHYQ YPGWAVAIGF
610 620 630 640 650
LMALSSVICI PLYALFQLCR TDGDTLLQRL KNATKPSRDW GPALLEHRTG
660 670 680 690
RYAPTTTPSP EDGFEVQPLH PDKAQIPIVG SNGSSRFQDS RI
Note: Produced by alternative promoter usage.
Length:692
Mass (Da):76,544
Last modified:August 10, 2010 - v3
Checksum:iC4A78A6562DAB01E
GO
Isoform GlyT-1A (identifier: P28571-1) [UniParc]FASTAAdd to basket

Also known as: GLYT1a

The sequence of this isoform differs from the canonical sequence as follows:
     1-69: MIGGDTRAAS...IAQHSPAMAQ → MVGKGAKGML

Note: Produced by alternative promoter usage.
Show »
Length:633
Mass (Da):70,558
Checksum:i51A0BD971F1BB794
GO
Isoform GlyT-1B (identifier: P28571-2) [UniParc]FASTAAdd to basket

Also known as: GLYT1b

The sequence of this isoform differs from the canonical sequence as follows:
     29-69: Missing.

Note: Produced by alternative splicing of isoform GlyT-1C.
Show »
Length:651
Mass (Da):72,231
Checksum:i47C32BDB76F52405
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti341 – 3411I → Y in CAA47440 (PubMed:1618338).Curated
Sequence conflicti341 – 3411I → Y in X82570 (PubMed:7891186).Curated
Sequence conflicti393 – 3942SV → RL in CAA47440 (PubMed:1618338).Curated
Sequence conflicti597 – 5971A → R in CAA47440 (PubMed:1618338).Curated
Sequence conflicti597 – 5971A → R in X82571 (PubMed:7891186).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 6969MIGGD…PAMAQ → MVGKGAKGML in isoform GlyT-1A. 3 PublicationsVSP_006272Add
BLAST
Alternative sequencei29 – 6941Missing in isoform GlyT-1B. 1 PublicationVSP_039241Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67056 mRNA. Translation: CAA47440.1.
X82567 Genomic DNA. No translation available.
X82568 Genomic DNA. No translation available.
X82569 Genomic DNA. No translation available.
X82570 Genomic DNA. No translation available.
X82571 Genomic DNA. No translation available.
X82572 Genomic DNA. No translation available.
AL627128 Genomic DNA. Translation: CAM14713.1.
BC021828 mRNA. Translation: AAH21828.1.
CCDSiCCDS18538.1. [P28571-1]
PIRiE46027.
S23151.
RefSeqiNP_032161.2. NM_008135.4. [P28571-1]
UniGeneiMm.244549.

Genome annotation databases

EnsembliENSMUST00000030269; ENSMUSP00000030269; ENSMUSG00000028542. [P28571-1]
ENSMUST00000063857; ENSMUSP00000066102; ENSMUSG00000028542. [P28571-1]
GeneIDi14664.
KEGGimmu:14664.
UCSCiuc008uiy.1. mouse. [P28571-1]

Keywords - Coding sequence diversityi

Alternative promoter usage, Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67056 mRNA. Translation: CAA47440.1.
X82567 Genomic DNA. No translation available.
X82568 Genomic DNA. No translation available.
X82569 Genomic DNA. No translation available.
X82570 Genomic DNA. No translation available.
X82571 Genomic DNA. No translation available.
X82572 Genomic DNA. No translation available.
AL627128 Genomic DNA. Translation: CAM14713.1.
BC021828 mRNA. Translation: AAH21828.1.
CCDSiCCDS18538.1. [P28571-1]
PIRiE46027.
S23151.
RefSeqiNP_032161.2. NM_008135.4. [P28571-1]
UniGeneiMm.244549.

3D structure databases

ProteinModelPortaliP28571.
SMRiP28571. Positions 86-639.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030269.

Chemistry

BindingDBiP28571.
ChEMBLiCHEMBL1075303.

PTM databases

PhosphoSiteiP28571.

Proteomic databases

MaxQBiP28571.
PaxDbiP28571.
PRIDEiP28571.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030269; ENSMUSP00000030269; ENSMUSG00000028542. [P28571-1]
ENSMUST00000063857; ENSMUSP00000066102; ENSMUSG00000028542. [P28571-1]
GeneIDi14664.
KEGGimmu:14664.
UCSCiuc008uiy.1. mouse. [P28571-1]

Organism-specific databases

CTDi6536.
MGIiMGI:95760. Slc6a9.

Phylogenomic databases

eggNOGiCOG0733.
GeneTreeiENSGT00760000118857.
HOGENOMiHOG000116406.
HOVERGENiHBG071421.
InParanoidiP28571.
KOiK05038.
OrthoDBiEOG793B71.
PhylomeDBiP28571.
TreeFamiTF343812.

Enzyme and pathway databases

ReactomeiREACT_307956. Na+/Cl- dependent neurotransmitter transporters.

Miscellaneous databases

NextBioi286542.
PROiP28571.
SOURCEiSearch...

Gene expression databases

BgeeiP28571.
ExpressionAtlasiP28571. baseline and differential.
GenevisibleiP28571. MM.

Family and domain databases

InterProiIPR000175. Na/ntran_symport.
IPR003028. Na/ntran_symport_glycine_GLY1.
[Graphical view]
PANTHERiPTHR11616. PTHR11616. 1 hit.
PfamiPF00209. SNF. 1 hit.
[Graphical view]
PRINTSiPR01204. GLY1TRNSPORT.
PR00176. NANEUSMPORT.
PROSITEiPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression of a glycine transporter from mouse brain."
    Liu Q.-R., Nelson H., Mandiyan S., Lopez-Corcuera B., Nelson N.
    FEBS Lett. 305:110-114(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM GLYT-1A).
    Tissue: Brain.
  2. "Gene structure and glial expression of the glycine transporter GlyT1 in embryonic and adult rodents."
    Adams R.H., Sato K., Shimada S., Tohyama M., Puschel A.W., Betz H.
    J. Neurosci. 15:2524-2532(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS GLYT-1A; GLYT-1B AND GLYT-1C), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, ALTERNATIVE PROMOTER USAGE, ALTERNATIVE SPLICING.
    Strain: NMRI.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM GLYT-1A).
    Strain: FVB/N.
    Tissue: Liver.
  5. "Cloning and expression of a spinal cord- and brain-specific glycine transporter with novel structural features."
    Liu Q.-R., Lopez-Corcuera B., Mandiyan S., Nelson H., Nelson N.
    J. Biol. Chem. 268:22802-22808(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-117 (ISOFORMS GLYT-1A AND GLYT-1B), TISSUE SPECIFICITY.
    Tissue: Brain.

Entry informationi

Entry nameiSC6A9_MOUSE
AccessioniPrimary (citable) accession number: P28571
Secondary accession number(s): B1ASI9, Q8VC47
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: August 10, 2010
Last modified: June 24, 2015
This is version 121 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.