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Protein

Sodium- and chloride-dependent glycine transporter 1

Gene

Slc6a9

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Terminates the action of glycine by its high affinity sodium-dependent reuptake into presynaptic terminals. May play a role in regulation of glycine levels in NMDA receptor-mediated neurotransmission.

GO - Molecular functioni

GO - Biological processi

  • glycine secretion, neurotransmission Source: MGI
  • glycine transport Source: MGI

Keywordsi

Biological processAmino-acid transport, Neurotransmitter transport, Symport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-442660 Na+/Cl- dependent neurotransmitter transporters

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium- and chloride-dependent glycine transporter 1
Short name:
GlyT-1
Short name:
GlyT1
Alternative name(s):
Solute carrier family 6 member 9
Gene namesi
Name:Slc6a9
Synonyms:Glyt1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:95760 Slc6a9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 94CytoplasmicSequence analysisAdd BLAST94
Transmembranei95 – 115Helical; Name=1Sequence analysisAdd BLAST21
Transmembranei122 – 142Helical; Name=2Sequence analysisAdd BLAST21
Transmembranei174 – 194Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini195 – 271ExtracellularSequence analysisAdd BLAST77
Transmembranei272 – 292Helical; Name=4Sequence analysisAdd BLAST21
Transmembranei301 – 321Helical; Name=5Sequence analysisAdd BLAST21
Transmembranei346 – 366Helical; Name=6Sequence analysisAdd BLAST21
Transmembranei393 – 413Helical; Name=7Sequence analysisAdd BLAST21
Transmembranei436 – 456Helical; Name=8Sequence analysisAdd BLAST21
Transmembranei492 – 512Helical; Name=9Sequence analysisAdd BLAST21
Transmembranei516 – 536Helical; Name=10Sequence analysisAdd BLAST21
Transmembranei556 – 576Helical; Name=11Sequence analysisAdd BLAST21
Transmembranei596 – 616Helical; Name=12Sequence analysisAdd BLAST21
Topological domaini617 – 692CytoplasmicSequence analysisAdd BLAST76

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1075303

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002147811 – 692Sodium- and chloride-dependent glycine transporter 1Add BLAST692

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei657PhosphothreonineCombined sources1
Modified residuei659PhosphoserineCombined sources1
Modified residuei684PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP28571
PRIDEiP28571

PTM databases

iPTMnetiP28571
PhosphoSitePlusiP28571

Expressioni

Tissue specificityi

Expressed in the brain (at protein level) (PubMed:27773429). At E11, expressed in the ventral part of the ventricular zone. At E15, also expressed in adjacent mantle tissue and the meninges. Strongly expressed in E12 and E15 liver.3 Publications

Developmental stagei

Expression is present at low levels as early as E9 and E10, but strongly increases at E13 and remains at high levels up to E15. Also expressed in adult.1 Publication

Gene expression databases

BgeeiENSMUSG00000028542
ExpressionAtlasiP28571 baseline and differential
GenevisibleiP28571 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030269

Chemistry databases

BindingDBiP28571

Structurei

3D structure databases

ProteinModelPortaliP28571
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3659 Eukaryota
COG0733 LUCA
GeneTreeiENSGT00760000118857
HOGENOMiHOG000116406
HOVERGENiHBG071421
InParanoidiP28571
KOiK05038
PhylomeDBiP28571
TreeFamiTF343812

Family and domain databases

InterProiView protein in InterPro
IPR000175 Na/ntran_symport
IPR003028 Na/ntran_symport_glycine_GLY1
IPR037272 SNS_sf
PANTHERiPTHR11616 PTHR11616, 1 hit
PfamiView protein in Pfam
PF00209 SNF, 1 hit
PRINTSiPR01204 GLY1TRNSPORT
PR00176 NANEUSMPORT
SUPFAMiSSF161070 SSF161070, 2 hits
PROSITEiView protein in PROSITE
PS00610 NA_NEUROTRAN_SYMP_1, 1 hit
PS00754 NA_NEUROTRAN_SYMP_2, 1 hit
PS50267 NA_NEUROTRAN_SYMP_3, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative promoter usage and alternative splicing. AlignAdd to basket

Isoform GlyT-1C (identifier: P28571-3) [UniParc]FASTAAdd to basket
Also known as: GLYT1c

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIGGDTRAAS AHPGMASAQG PVATPSPEQP FPGTTSVSLA RPVLRVWHGA
60 70 80 90 100
HSSGLLPNLI AQHSPAMAQN GAVPSEATKK DQNLTRGNWG NQIEFVLTSV
110 120 130 140 150
GYAVGLGNVW RFPYLCYRNG GGAFMFPYFI MLIFCGIPLF FMELSFGQFA
160 170 180 190 200
SQGCLGVWRI SPMFKGVGYG MMVVSTYIGI YYNVVICIAF YYFFSSMTHV
210 220 230 240 250
LPWAYCNNPW NTPDCAGVLD ASNLTNGSRP AALSGNLSHL FNYTLQRTSP
260 270 280 290 300
SEEYWRLYVL KLSDDIGNFG EVRLPLLGCL GVSWVVVFLC LIRGVKSSGK
310 320 330 340 350
VVYFTATFPY VVLTILFVRG VTLEGAFTGI MYYLTPQWDK ILEAKVWGDA
360 370 380 390 400
ASQIFYSLGC AWGGLITMAS YNKFHNNCYR DSVIISITNC ATSVYAGFVI
410 420 430 440 450
FSILGFMANH LGVDVSRVAD HGPGLAFVAY PEALTLLPIS PLWSLLFFFM
460 470 480 490 500
LILLGLGTQF CLLETLVTAI VDEVGNEWIL QKKTYVTLGV AVAGFLLGIP
510 520 530 540 550
LTSQAGIYWL LLMDNYAASF SLVVISCIMC VSIMYIYGHR NYFQDIQMML
560 570 580 590 600
GFPPPLFFQI CWRFVSPAII FFILIFTVIQ YRPITYNHYQ YPGWAVAIGF
610 620 630 640 650
LMALSSVICI PLYALFQLCR TDGDTLLQRL KNATKPSRDW GPALLEHRTG
660 670 680 690
RYAPTTTPSP EDGFEVQPLH PDKAQIPIVG SNGSSRFQDS RI
Note: Produced by alternative promoter usage.
Length:692
Mass (Da):76,544
Last modified:August 10, 2010 - v3
Checksum:iC4A78A6562DAB01E
GO
Isoform GlyT-1A (identifier: P28571-1) [UniParc]FASTAAdd to basket
Also known as: GLYT1a

The sequence of this isoform differs from the canonical sequence as follows:
     1-69: MIGGDTRAAS...IAQHSPAMAQ → MVGKGAKGML

Note: Produced by alternative promoter usage.
Show »
Length:633
Mass (Da):70,558
Checksum:i51A0BD971F1BB794
GO
Isoform GlyT-1B (identifier: P28571-2) [UniParc]FASTAAdd to basket
Also known as: GLYT1b

The sequence of this isoform differs from the canonical sequence as follows:
     29-69: Missing.

Note: Produced by alternative splicing of isoform GlyT-1C.
Show »
Length:651
Mass (Da):72,231
Checksum:i47C32BDB76F52405
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti341I → Y in CAA47440 (PubMed:1618338).Curated1
Sequence conflicti341I → Y in X82570 (PubMed:7891186).Curated1
Sequence conflicti393 – 394SV → RL in CAA47440 (PubMed:1618338).Curated2
Sequence conflicti597A → R in CAA47440 (PubMed:1618338).Curated1
Sequence conflicti597A → R in X82571 (PubMed:7891186).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0062721 – 69MIGGD…PAMAQ → MVGKGAKGML in isoform GlyT-1A. 3 PublicationsAdd BLAST69
Alternative sequenceiVSP_03924129 – 69Missing in isoform GlyT-1B. 1 PublicationAdd BLAST41

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67056 mRNA Translation: CAA47440.1
X82567 Genomic DNA No translation available.
X82568 Genomic DNA No translation available.
X82569 Genomic DNA No translation available.
X82570 Genomic DNA No translation available.
X82571 Genomic DNA No translation available.
X82572 Genomic DNA No translation available.
AL627128 Genomic DNA Translation: CAM14713.1
BC021828 mRNA Translation: AAH21828.1
CCDSiCCDS18538.1 [P28571-1]
PIRiE46027
S23151
RefSeqiNP_032161.2, NM_008135.4 [P28571-1]
UniGeneiMm.244549
Mm.440118

Genome annotation databases

EnsembliENSMUST00000030269; ENSMUSP00000030269; ENSMUSG00000028542 [P28571-1]
ENSMUST00000063857; ENSMUSP00000066102; ENSMUSG00000028542 [P28571-1]
GeneIDi14664
KEGGimmu:14664
UCSCiuc008uiy.1 mouse [P28571-1]

Keywords - Coding sequence diversityi

Alternative promoter usage, Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSC6A9_MOUSE
AccessioniPrimary (citable) accession number: P28571
Secondary accession number(s): B1ASI9, Q8VC47
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: August 10, 2010
Last modified: April 25, 2018
This is version 138 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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