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P28571 (SC6A9_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sodium- and chloride-dependent glycine transporter 1

Short name=GlyT-1
Short name=GlyT1
Alternative name(s):
Solute carrier family 6 member 9
Gene names
Name:Slc6a9
Synonyms:Glyt1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length692 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Terminates the action of glycine by its high affinity sodium-dependent reuptake into presynaptic terminals. May play a role in regulation of glycine levels in NMDA receptor-mediated neurotransmission.

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

At E11, expressed in the ventral part of the ventricular zone. At E15, also expressed in adjacent mantle tissue and the meninges. Strongly expressed in E12 and E15 liver. Ref.2 Ref.5

Developmental stage

Expression is present at low levels as early as E9 and E10, but strongly increases at E13 and remains at high levels up to E15. Also expressed in adult. Ref.2

Sequence similarities

Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A9 subfamily. [View classification]

Alternative products

This entry describes 3 isoforms produced by alternative promoter usage and alternative splicing. [Align] [Select]
Isoform GlyT-1C (identifier: P28571-3)

Also known as: GLYT1c;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Produced by alternative promoter usage.
Isoform GlyT-1A (identifier: P28571-1)

Also known as: GLYT1a;

The sequence of this isoform differs from the canonical sequence as follows:
     1-69: MIGGDTRAAS...IAQHSPAMAQ → MVGKGAKGML
Note: Produced by alternative promoter usage.
Isoform GlyT-1B (identifier: P28571-2)

Also known as: GLYT1b;

The sequence of this isoform differs from the canonical sequence as follows:
     29-69: Missing.
Note: Produced by alternative splicing of isoform GlyT-1C.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 692692Sodium- and chloride-dependent glycine transporter 1
PRO_0000214781

Regions

Topological domain1 – 9494Cytoplasmic Potential
Transmembrane95 – 11521Helical; Name=1; Potential
Transmembrane122 – 14221Helical; Name=2; Potential
Transmembrane174 – 19421Helical; Name=3; Potential
Topological domain195 – 27177Extracellular Potential
Transmembrane272 – 29221Helical; Name=4; Potential
Transmembrane301 – 32121Helical; Name=5; Potential
Transmembrane346 – 36621Helical; Name=6; Potential
Transmembrane393 – 41321Helical; Name=7; Potential
Transmembrane436 – 45621Helical; Name=8; Potential
Transmembrane492 – 51221Helical; Name=9; Potential
Transmembrane516 – 53621Helical; Name=10; Potential
Transmembrane556 – 57621Helical; Name=11; Potential
Transmembrane596 – 61621Helical; Name=12; Potential
Topological domain617 – 69276Cytoplasmic Potential

Natural variations

Alternative sequence1 – 6969MIGGD…PAMAQ → MVGKGAKGML in isoform GlyT-1A.
VSP_006272
Alternative sequence29 – 6941Missing in isoform GlyT-1B.
VSP_039241

Experimental info

Sequence conflict3411I → Y in CAA47440. Ref.1
Sequence conflict3411I → Y in X82570. Ref.2
Sequence conflict393 – 3942SV → RL in CAA47440. Ref.1
Sequence conflict5971A → R in CAA47440. Ref.1
Sequence conflict5971A → R in X82571. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform GlyT-1C (GLYT1c) [UniParc].

Last modified August 10, 2010. Version 3.
Checksum: C4A78A6562DAB01E

FASTA69276,544
        10         20         30         40         50         60 
MIGGDTRAAS AHPGMASAQG PVATPSPEQP FPGTTSVSLA RPVLRVWHGA HSSGLLPNLI 

        70         80         90        100        110        120 
AQHSPAMAQN GAVPSEATKK DQNLTRGNWG NQIEFVLTSV GYAVGLGNVW RFPYLCYRNG 

       130        140        150        160        170        180 
GGAFMFPYFI MLIFCGIPLF FMELSFGQFA SQGCLGVWRI SPMFKGVGYG MMVVSTYIGI 

       190        200        210        220        230        240 
YYNVVICIAF YYFFSSMTHV LPWAYCNNPW NTPDCAGVLD ASNLTNGSRP AALSGNLSHL 

       250        260        270        280        290        300 
FNYTLQRTSP SEEYWRLYVL KLSDDIGNFG EVRLPLLGCL GVSWVVVFLC LIRGVKSSGK 

       310        320        330        340        350        360 
VVYFTATFPY VVLTILFVRG VTLEGAFTGI MYYLTPQWDK ILEAKVWGDA ASQIFYSLGC 

       370        380        390        400        410        420 
AWGGLITMAS YNKFHNNCYR DSVIISITNC ATSVYAGFVI FSILGFMANH LGVDVSRVAD 

       430        440        450        460        470        480 
HGPGLAFVAY PEALTLLPIS PLWSLLFFFM LILLGLGTQF CLLETLVTAI VDEVGNEWIL 

       490        500        510        520        530        540 
QKKTYVTLGV AVAGFLLGIP LTSQAGIYWL LLMDNYAASF SLVVISCIMC VSIMYIYGHR 

       550        560        570        580        590        600 
NYFQDIQMML GFPPPLFFQI CWRFVSPAII FFILIFTVIQ YRPITYNHYQ YPGWAVAIGF 

       610        620        630        640        650        660 
LMALSSVICI PLYALFQLCR TDGDTLLQRL KNATKPSRDW GPALLEHRTG RYAPTTTPSP 

       670        680        690 
EDGFEVQPLH PDKAQIPIVG SNGSSRFQDS RI 

« Hide

Isoform GlyT-1A (GLYT1a) [UniParc].

Checksum: 51A0BD971F1BB794
Show »

FASTA63370,558
Isoform GlyT-1B (GLYT1b) [UniParc].

Checksum: 47C32BDB76F52405
Show »

FASTA65172,231

References

« Hide 'large scale' references
[1]"Cloning and expression of a glycine transporter from mouse brain."
Liu Q.-R., Nelson H., Mandiyan S., Lopez-Corcuera B., Nelson N.
FEBS Lett. 305:110-114(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM GLYT-1A).
Tissue: Brain.
[2]"Gene structure and glial expression of the glycine transporter GlyT1 in embryonic and adult rodents."
Adams R.H., Sato K., Shimada S., Tohyama M., Puschel A.W., Betz H.
J. Neurosci. 15:2524-2532(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS GLYT-1A; GLYT-1B AND GLYT-1C), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, ALTERNATIVE PROMOTER USAGE, ALTERNATIVE SPLICING.
Strain: NMRI.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM GLYT-1A).
Strain: FVB/N.
Tissue: Liver.
[5]"Cloning and expression of a spinal cord- and brain-specific glycine transporter with novel structural features."
Liu Q.-R., Lopez-Corcuera B., Mandiyan S., Nelson H., Nelson N.
J. Biol. Chem. 268:22802-22808(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-117 (ISOFORMS GLYT-1A AND GLYT-1B), TISSUE SPECIFICITY.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X67056 mRNA. Translation: CAA47440.1.
X82567 Genomic DNA. No translation available.
X82568 Genomic DNA. No translation available.
X82569 Genomic DNA. No translation available.
X82570 Genomic DNA. No translation available.
X82571 Genomic DNA. No translation available.
X82572 Genomic DNA. No translation available.
AL627128 Genomic DNA. Translation: CAM14713.1.
BC021828 mRNA. Translation: AAH21828.1.
CCDSCCDS18538.1. [P28571-1]
PIRE46027.
S23151.
RefSeqNP_032161.2. NM_008135.4. [P28571-1]
UniGeneMm.244549.

3D structure databases

ProteinModelPortalP28571.
SMRP28571. Positions 86-639.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000099746.

Chemistry

ChEMBLCHEMBL1075303.

PTM databases

PhosphoSiteP28571.

Proteomic databases

PaxDbP28571.
PRIDEP28571.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000030269; ENSMUSP00000030269; ENSMUSG00000028542. [P28571-1]
ENSMUST00000063857; ENSMUSP00000066102; ENSMUSG00000028542. [P28571-1]
GeneID14664.
KEGGmmu:14664.
UCSCuc008uiy.1. mouse. [P28571-1]

Organism-specific databases

CTD6536.
MGIMGI:95760. Slc6a9.

Phylogenomic databases

eggNOGCOG0733.
GeneTreeENSGT00740000115427.
HOGENOMHOG000116406.
HOVERGENHBG071421.
KOK05038.
OrthoDBEOG793B71.
PhylomeDBP28571.
TreeFamTF343812.

Gene expression databases

ArrayExpressP28571.
BgeeP28571.
GenevestigatorP28571.

Family and domain databases

InterProIPR000175. Na/ntran_symport.
IPR003028. Na/ntran_symport_glycine_GLY1.
[Graphical view]
PANTHERPTHR11616. PTHR11616. 1 hit.
PfamPF00209. SNF. 1 hit.
[Graphical view]
PRINTSPR01204. GLY1TRNSPORT.
PR00176. NANEUSMPORT.
PROSITEPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio286542.
PROP28571.
SOURCESearch...

Entry information

Entry nameSC6A9_MOUSE
AccessionPrimary (citable) accession number: P28571
Secondary accession number(s): B1ASI9, Q8VC47
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: August 10, 2010
Last modified: July 9, 2014
This is version 115 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot