Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P28571

- SC6A9_MOUSE

UniProt

P28571 - SC6A9_MOUSE

Protein

Sodium- and chloride-dependent glycine transporter 1

Gene

Slc6a9

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 3 (10 Aug 2010)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Terminates the action of glycine by its high affinity sodium-dependent reuptake into presynaptic terminals. May play a role in regulation of glycine levels in NMDA receptor-mediated neurotransmission.

    GO - Molecular functioni

    1. glycine transmembrane transporter activity Source: MGI
    2. neurotransmitter:sodium symporter activity Source: InterPro
    3. sodium:amino acid symporter activity Source: InterPro

    GO - Biological processi

    1. glycine transport Source: MGI

    Keywords - Biological processi

    Amino-acid transport, Neurotransmitter transport, Symport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium- and chloride-dependent glycine transporter 1
    Short name:
    GlyT-1
    Short name:
    GlyT1
    Alternative name(s):
    Solute carrier family 6 member 9
    Gene namesi
    Name:Slc6a9
    Synonyms:Glyt1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:95760. Slc6a9.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: MGI
    2. integral component of plasma membrane Source: InterPro
    3. plasma membrane Source: MGI

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 692692Sodium- and chloride-dependent glycine transporter 1PRO_0000214781Add
    BLAST

    Proteomic databases

    PaxDbiP28571.
    PRIDEiP28571.

    PTM databases

    PhosphoSiteiP28571.

    Expressioni

    Tissue specificityi

    At E11, expressed in the ventral part of the ventricular zone. At E15, also expressed in adjacent mantle tissue and the meninges. Strongly expressed in E12 and E15 liver.2 Publications

    Developmental stagei

    Expression is present at low levels as early as E9 and E10, but strongly increases at E13 and remains at high levels up to E15. Also expressed in adult.1 Publication

    Gene expression databases

    ArrayExpressiP28571.
    BgeeiP28571.
    GenevestigatoriP28571.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000099746.

    Structurei

    3D structure databases

    ProteinModelPortaliP28571.
    SMRiP28571. Positions 86-639.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 9494CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini195 – 27177ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini617 – 69276CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei95 – 11521Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei122 – 14221Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei174 – 19421Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei272 – 29221Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei301 – 32121Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei346 – 36621Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei393 – 41321Helical; Name=7Sequence AnalysisAdd
    BLAST
    Transmembranei436 – 45621Helical; Name=8Sequence AnalysisAdd
    BLAST
    Transmembranei492 – 51221Helical; Name=9Sequence AnalysisAdd
    BLAST
    Transmembranei516 – 53621Helical; Name=10Sequence AnalysisAdd
    BLAST
    Transmembranei556 – 57621Helical; Name=11Sequence AnalysisAdd
    BLAST
    Transmembranei596 – 61621Helical; Name=12Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0733.
    GeneTreeiENSGT00740000115427.
    HOGENOMiHOG000116406.
    HOVERGENiHBG071421.
    KOiK05038.
    OrthoDBiEOG793B71.
    PhylomeDBiP28571.
    TreeFamiTF343812.

    Family and domain databases

    InterProiIPR000175. Na/ntran_symport.
    IPR003028. Na/ntran_symport_glycine_GLY1.
    [Graphical view]
    PANTHERiPTHR11616. PTHR11616. 1 hit.
    PfamiPF00209. SNF. 1 hit.
    [Graphical view]
    PRINTSiPR01204. GLY1TRNSPORT.
    PR00176. NANEUSMPORT.
    PROSITEiPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
    PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
    PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative promoter usage and alternative splicing. Align

    Isoform GlyT-1C (identifier: P28571-3) [UniParc]FASTAAdd to Basket

    Also known as: GLYT1c

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MIGGDTRAAS AHPGMASAQG PVATPSPEQP FPGTTSVSLA RPVLRVWHGA    50
    HSSGLLPNLI AQHSPAMAQN GAVPSEATKK DQNLTRGNWG NQIEFVLTSV 100
    GYAVGLGNVW RFPYLCYRNG GGAFMFPYFI MLIFCGIPLF FMELSFGQFA 150
    SQGCLGVWRI SPMFKGVGYG MMVVSTYIGI YYNVVICIAF YYFFSSMTHV 200
    LPWAYCNNPW NTPDCAGVLD ASNLTNGSRP AALSGNLSHL FNYTLQRTSP 250
    SEEYWRLYVL KLSDDIGNFG EVRLPLLGCL GVSWVVVFLC LIRGVKSSGK 300
    VVYFTATFPY VVLTILFVRG VTLEGAFTGI MYYLTPQWDK ILEAKVWGDA 350
    ASQIFYSLGC AWGGLITMAS YNKFHNNCYR DSVIISITNC ATSVYAGFVI 400
    FSILGFMANH LGVDVSRVAD HGPGLAFVAY PEALTLLPIS PLWSLLFFFM 450
    LILLGLGTQF CLLETLVTAI VDEVGNEWIL QKKTYVTLGV AVAGFLLGIP 500
    LTSQAGIYWL LLMDNYAASF SLVVISCIMC VSIMYIYGHR NYFQDIQMML 550
    GFPPPLFFQI CWRFVSPAII FFILIFTVIQ YRPITYNHYQ YPGWAVAIGF 600
    LMALSSVICI PLYALFQLCR TDGDTLLQRL KNATKPSRDW GPALLEHRTG 650
    RYAPTTTPSP EDGFEVQPLH PDKAQIPIVG SNGSSRFQDS RI 692

    Note: Produced by alternative promoter usage.

    Length:692
    Mass (Da):76,544
    Last modified:August 10, 2010 - v3
    Checksum:iC4A78A6562DAB01E
    GO
    Isoform GlyT-1A (identifier: P28571-1) [UniParc]FASTAAdd to Basket

    Also known as: GLYT1a

    The sequence of this isoform differs from the canonical sequence as follows:
         1-69: MIGGDTRAAS...IAQHSPAMAQ → MVGKGAKGML

    Note: Produced by alternative promoter usage.

    Show »
    Length:633
    Mass (Da):70,558
    Checksum:i51A0BD971F1BB794
    GO
    Isoform GlyT-1B (identifier: P28571-2) [UniParc]FASTAAdd to Basket

    Also known as: GLYT1b

    The sequence of this isoform differs from the canonical sequence as follows:
         29-69: Missing.

    Note: Produced by alternative splicing of isoform GlyT-1C.

    Show »
    Length:651
    Mass (Da):72,231
    Checksum:i47C32BDB76F52405
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti341 – 3411I → Y in CAA47440. (PubMed:1618338)Curated
    Sequence conflicti341 – 3411I → Y in X82570. (PubMed:7891186)Curated
    Sequence conflicti393 – 3942SV → RL in CAA47440. (PubMed:1618338)Curated
    Sequence conflicti597 – 5971A → R in CAA47440. (PubMed:1618338)Curated
    Sequence conflicti597 – 5971A → R in X82571. (PubMed:7891186)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 6969MIGGD…PAMAQ → MVGKGAKGML in isoform GlyT-1A. 3 PublicationsVSP_006272Add
    BLAST
    Alternative sequencei29 – 6941Missing in isoform GlyT-1B. 1 PublicationVSP_039241Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X67056 mRNA. Translation: CAA47440.1.
    X82567 Genomic DNA. No translation available.
    X82568 Genomic DNA. No translation available.
    X82569 Genomic DNA. No translation available.
    X82570 Genomic DNA. No translation available.
    X82571 Genomic DNA. No translation available.
    X82572 Genomic DNA. No translation available.
    AL627128 Genomic DNA. Translation: CAM14713.1.
    BC021828 mRNA. Translation: AAH21828.1.
    CCDSiCCDS18538.1. [P28571-1]
    PIRiE46027.
    S23151.
    RefSeqiNP_032161.2. NM_008135.4. [P28571-1]
    UniGeneiMm.244549.

    Genome annotation databases

    EnsembliENSMUST00000030269; ENSMUSP00000030269; ENSMUSG00000028542. [P28571-1]
    ENSMUST00000063857; ENSMUSP00000066102; ENSMUSG00000028542. [P28571-1]
    GeneIDi14664.
    KEGGimmu:14664.
    UCSCiuc008uiy.1. mouse. [P28571-1]

    Keywords - Coding sequence diversityi

    Alternative promoter usage, Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X67056 mRNA. Translation: CAA47440.1 .
    X82567 Genomic DNA. No translation available.
    X82568 Genomic DNA. No translation available.
    X82569 Genomic DNA. No translation available.
    X82570 Genomic DNA. No translation available.
    X82571 Genomic DNA. No translation available.
    X82572 Genomic DNA. No translation available.
    AL627128 Genomic DNA. Translation: CAM14713.1 .
    BC021828 mRNA. Translation: AAH21828.1 .
    CCDSi CCDS18538.1. [P28571-1 ]
    PIRi E46027.
    S23151.
    RefSeqi NP_032161.2. NM_008135.4. [P28571-1 ]
    UniGenei Mm.244549.

    3D structure databases

    ProteinModelPortali P28571.
    SMRi P28571. Positions 86-639.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000099746.

    Chemistry

    ChEMBLi CHEMBL1075303.

    PTM databases

    PhosphoSitei P28571.

    Proteomic databases

    PaxDbi P28571.
    PRIDEi P28571.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000030269 ; ENSMUSP00000030269 ; ENSMUSG00000028542 . [P28571-1 ]
    ENSMUST00000063857 ; ENSMUSP00000066102 ; ENSMUSG00000028542 . [P28571-1 ]
    GeneIDi 14664.
    KEGGi mmu:14664.
    UCSCi uc008uiy.1. mouse. [P28571-1 ]

    Organism-specific databases

    CTDi 6536.
    MGIi MGI:95760. Slc6a9.

    Phylogenomic databases

    eggNOGi COG0733.
    GeneTreei ENSGT00740000115427.
    HOGENOMi HOG000116406.
    HOVERGENi HBG071421.
    KOi K05038.
    OrthoDBi EOG793B71.
    PhylomeDBi P28571.
    TreeFami TF343812.

    Miscellaneous databases

    NextBioi 286542.
    PROi P28571.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P28571.
    Bgeei P28571.
    Genevestigatori P28571.

    Family and domain databases

    InterProi IPR000175. Na/ntran_symport.
    IPR003028. Na/ntran_symport_glycine_GLY1.
    [Graphical view ]
    PANTHERi PTHR11616. PTHR11616. 1 hit.
    Pfami PF00209. SNF. 1 hit.
    [Graphical view ]
    PRINTSi PR01204. GLY1TRNSPORT.
    PR00176. NANEUSMPORT.
    PROSITEi PS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
    PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
    PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of a glycine transporter from mouse brain."
      Liu Q.-R., Nelson H., Mandiyan S., Lopez-Corcuera B., Nelson N.
      FEBS Lett. 305:110-114(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM GLYT-1A).
      Tissue: Brain.
    2. "Gene structure and glial expression of the glycine transporter GlyT1 in embryonic and adult rodents."
      Adams R.H., Sato K., Shimada S., Tohyama M., Puschel A.W., Betz H.
      J. Neurosci. 15:2524-2532(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS GLYT-1A; GLYT-1B AND GLYT-1C), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, ALTERNATIVE PROMOTER USAGE, ALTERNATIVE SPLICING.
      Strain: NMRI.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM GLYT-1A).
      Strain: FVB/N.
      Tissue: Liver.
    5. "Cloning and expression of a spinal cord- and brain-specific glycine transporter with novel structural features."
      Liu Q.-R., Lopez-Corcuera B., Mandiyan S., Nelson H., Nelson N.
      J. Biol. Chem. 268:22802-22808(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-117 (ISOFORMS GLYT-1A AND GLYT-1B), TISSUE SPECIFICITY.
      Tissue: Brain.

    Entry informationi

    Entry nameiSC6A9_MOUSE
    AccessioniPrimary (citable) accession number: P28571
    Secondary accession number(s): B1ASI9, Q8VC47
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: August 10, 2010
    Last modified: October 1, 2014
    This is version 116 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3