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Protein

Sodium- and chloride-dependent creatine transporter 1

Gene

Slc6a8

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Required for the uptake of creatine. Plays an important role in supplying creatine to the brain via the blood-brain barrier (By similarity).By similarity

GO - Molecular functioni

  • choline transmembrane transporter activity Source: RGD
  • creatine:sodium symporter activity Source: RGD
  • creatine transmembrane transporter activity Source: RGD
  • neurotransmitter:sodium symporter activity Source: InterPro

GO - Biological processi

  • creatine transport Source: RGD
  • embryonic brain development Source: RGD
Complete GO annotation...

Keywords - Biological processi

Ion transport, Sodium transport, Symport, Transport

Keywords - Ligandi

Sodium

Enzyme and pathway databases

ReactomeiR-RNO-71288. Creatine metabolism.

Protein family/group databases

TCDBi2.A.22.3.5. the neurotransmitter:sodium symporter (nss) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium- and chloride-dependent creatine transporter 1
Short name:
CHOT1
Short name:
CT1
Short name:
Creatine transporter 1
Alternative name(s):
Solute carrier family 6 member 8
Gene namesi
Name:Slc6a8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome X

Organism-specific databases

RGDi619711. Slc6a8.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6060CytoplasmicSequence analysisAdd
BLAST
Transmembranei61 – 8121HelicalSequence analysisAdd
BLAST
Topological domaini82 – 876ExtracellularSequence analysis
Transmembranei88 – 10821HelicalSequence analysisAdd
BLAST
Topological domaini109 – 13830CytoplasmicSequence analysisAdd
BLAST
Transmembranei139 – 15921HelicalSequence analysisAdd
BLAST
Topological domaini160 – 23071ExtracellularSequence analysisAdd
BLAST
Transmembranei231 – 25121HelicalSequence analysisAdd
BLAST
Topological domaini252 – 26918CytoplasmicSequence analysisAdd
BLAST
Transmembranei270 – 29021HelicalSequence analysisAdd
BLAST
Topological domaini291 – 30414ExtracellularSequence analysisAdd
BLAST
Transmembranei305 – 32521HelicalSequence analysisAdd
BLAST
Topological domaini326 – 34116CytoplasmicSequence analysisAdd
BLAST
Transmembranei342 – 36221HelicalSequence analysisAdd
BLAST
Topological domaini363 – 39432ExtracellularSequence analysisAdd
BLAST
Transmembranei395 – 41521HelicalSequence analysisAdd
BLAST
Topological domaini416 – 44429CytoplasmicSequence analysisAdd
BLAST
Transmembranei445 – 46521HelicalSequence analysisAdd
BLAST
Topological domaini466 – 47914ExtracellularSequence analysisAdd
BLAST
Transmembranei480 – 50021HelicalSequence analysisAdd
BLAST
Topological domaini501 – 52020CytoplasmicSequence analysisAdd
BLAST
Transmembranei521 – 54121HelicalSequence analysisAdd
BLAST
Topological domaini542 – 56019ExtracellularSequence analysisAdd
BLAST
Transmembranei561 – 58121HelicalSequence analysisAdd
BLAST
Topological domaini582 – 63554CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL3814.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 635635Sodium- and chloride-dependent creatine transporter 1PRO_0000214777Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi192 – 1921N-linked (GlcNAc...)Sequence analysis
Glycosylationi197 – 1971N-linked (GlcNAc...)Sequence analysis
Glycosylationi548 – 5481N-linked (GlcNAc...)Sequence analysis
Modified residuei617 – 6171PhosphothreonineBy similarity
Modified residuei620 – 6201PhosphothreonineBy similarity
Modified residuei623 – 6231PhosphoserineBy similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP28570.

PTM databases

iPTMnetiP28570.

Expressioni

Tissue specificityi

Expressed in brain, cerebellum, spinal cord, to a lesser extent, heart, but only very low expression in lung, kidney, and muscle.

Gene expression databases

ExpressionAtlasiP28570. differential.
GenevisibleiP28570. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025702.

Chemistry

BindingDBiP28570.

Structurei

3D structure databases

ProteinModelPortaliP28570.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3659. Eukaryota.
COG0733. LUCA.
GeneTreeiENSGT00760000118857.
HOGENOMiHOG000116406.
HOVERGENiHBG071421.
InParanoidiP28570.
KOiK05039.
OMAiVPPRETW.
OrthoDBiEOG793B71.
PhylomeDBiP28570.

Family and domain databases

InterProiIPR000175. Na/ntran_symport.
IPR002984. Na/ntran_symport_creatine.
[Graphical view]
PANTHERiPTHR11616. PTHR11616. 1 hit.
PfamiPF00209. SNF. 1 hit.
[Graphical view]
PRINTSiPR01199. CRTTRANSPORT.
PR00176. NANEUSMPORT.
PROSITEiPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28570-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKKSAENGI YSVSGDEKKG PLIVSGPDGA PSKGDGPAGL GAPSSRLAVP
60 70 80 90 100
PRETWTRQMD FIMSCVGFAV GLGNVWRFPY LCYKNGGGVF LIPYVLIALV
110 120 130 140 150
GGIPIFFLEI SLGQFMKAGS INVWNICPLF KGLGYASMVI VFYCNTYYIM
160 170 180 190 200
VLAWGFYYLV KSFTTTLPWA TCGHTWNTPD CVEIFRHEDC ANASLANLTC
210 220 230 240 250
DQLADRRSPV IEFWENKVLR LSTGLEVPGA LNWEVTLCLL ACWVLVYFCV
260 270 280 290 300
WKGVKSTGKI VYFTATFPYV VLVVLLVRGV LLPGALDGII YYLKPDWSKL
310 320 330 340 350
GSPQVWIDAG TQIFFSYAIG LGALTALGSY NRFNNNCYKD AIILALINSG
360 370 380 390 400
TSFFAGFVVF SILGFMATEQ GVHISKVAES GPGLAFIAYP RAVTLMPVAP
410 420 430 440 450
LWAALFFFML LLLGLDSQFV GVEGFITGLL DLLPASYYFR FQREISVALC
460 470 480 490 500
CALCFVIDLS MVTDGGMYVF QLFDYYSASG TTLLWQAFWE CVVVAWVYGA
510 520 530 540 550
DRFMDDIACM IGYRPCPWMK WCWSFFTPLV CMGIFIFNVV YYKPLVYNNT
560 570 580 590 600
YVYPWWGEAM GWAFALSSML CVPLHLLGCL LRAKGTMAER WQHLTQPIWG
610 620 630
LHHLEYRAQD ADVRGLTTLT PVSESSKVVV VESVM
Length:635
Mass (Da):70,632
Last modified:December 1, 1992 - v1
Checksum:i5D6A944035103787
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66494 mRNA. Translation: CAA47119.1.
PIRiS23431.
RefSeqiNP_059044.1. NM_017348.2.
UniGeneiRn.10336.

Genome annotation databases

EnsembliENSRNOT00000092123; ENSRNOP00000074536; ENSRNOG00000057620.
GeneIDi50690.
KEGGirno:50690.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66494 mRNA. Translation: CAA47119.1.
PIRiS23431.
RefSeqiNP_059044.1. NM_017348.2.
UniGeneiRn.10336.

3D structure databases

ProteinModelPortaliP28570.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025702.

Chemistry

BindingDBiP28570.
ChEMBLiCHEMBL3814.

Protein family/group databases

TCDBi2.A.22.3.5. the neurotransmitter:sodium symporter (nss) family.

PTM databases

iPTMnetiP28570.

Proteomic databases

PaxDbiP28570.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000092123; ENSRNOP00000074536; ENSRNOG00000057620.
GeneIDi50690.
KEGGirno:50690.

Organism-specific databases

CTDi6535.
RGDi619711. Slc6a8.

Phylogenomic databases

eggNOGiKOG3659. Eukaryota.
COG0733. LUCA.
GeneTreeiENSGT00760000118857.
HOGENOMiHOG000116406.
HOVERGENiHBG071421.
InParanoidiP28570.
KOiK05039.
OMAiVPPRETW.
OrthoDBiEOG793B71.
PhylomeDBiP28570.

Enzyme and pathway databases

ReactomeiR-RNO-71288. Creatine metabolism.

Miscellaneous databases

PROiP28570.

Gene expression databases

ExpressionAtlasiP28570. differential.
GenevisibleiP28570. RN.

Family and domain databases

InterProiIPR000175. Na/ntran_symport.
IPR002984. Na/ntran_symport_creatine.
[Graphical view]
PANTHERiPTHR11616. PTHR11616. 1 hit.
PfamiPF00209. SNF. 1 hit.
[Graphical view]
PRINTSiPR01199. CRTTRANSPORT.
PR00176. NANEUSMPORT.
PROSITEiPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Primary structure and functional expression of a choline transporter expressed in the rat nervous system."
    Mayser W., Schloss P., Betz H.
    FEBS Lett. 305:31-36(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Brain.

Entry informationi

Entry nameiSC6A8_RAT
AccessioniPrimary (citable) accession number: P28570
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: July 6, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be a choline transporter.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.