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P28566

- 5HT1E_HUMAN

UniProt

P28566 - 5HT1E_HUMAN

Protein

5-hydroxytryptamine receptor 1E

Gene

HTR1E

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 139 (01 Oct 2014)
      Sequence version 1 (01 Dec 1992)
      Previous versions | rss
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    Functioni

    G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various alkaloids and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity.5 Publications

    GO - Molecular functioni

    1. G-protein coupled receptor activity Source: ProtInc
    2. serotonin binding Source: MGI
    3. serotonin receptor activity Source: UniProtKB

    GO - Biological processi

    1. adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway Source: UniProtKB
    2. G-protein coupled receptor signaling pathway Source: ProtInc
    3. G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger Source: ProtInc
    4. negative regulation of cAMP metabolic process Source: InterPro
    5. serotonin receptor signaling pathway Source: GOC
    6. synaptic transmission Source: ProtInc

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Enzyme and pathway databases

    ReactomeiREACT_17064. Serotonin receptors.
    REACT_19231. G alpha (i) signalling events.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    5-hydroxytryptamine receptor 1E
    Short name:
    5-HT-1E
    Short name:
    5-HT1E
    Alternative name(s):
    S31
    Serotonin receptor 1E
    Gene namesi
    Name:HTR1E
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 6

    Organism-specific databases

    HGNCiHGNC:5291. HTR1E.

    Subcellular locationi

    Cell membrane 5 Publications; Multi-pass membrane protein 5 Publications

    GO - Cellular componenti

    1. integral component of plasma membrane Source: UniProtKB
    2. plasma membrane Source: Reactome

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA29552.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 3653655-hydroxytryptamine receptor 1EPRO_0000068933Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi2 – 21N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi5 – 51N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi95 ↔ 173PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiP28566.
    PRIDEiP28566.

    Expressioni

    Tissue specificityi

    Detected in brain.1 Publication

    Gene expression databases

    BgeeiP28566.
    CleanExiHS_HTR1E.
    GenevestigatoriP28566.

    Organism-specific databases

    HPAiHPA004931.

    Interactioni

    Protein-protein interaction databases

    BioGridi109586. 2 interactions.
    STRINGi9606.ENSP00000307766.

    Structurei

    3D structure databases

    ProteinModelPortaliP28566.
    SMRiP28566. Positions 22-360.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2222ExtracellularBy similarityAdd
    BLAST
    Topological domaini48 – 5912CytoplasmicBy similarityAdd
    BLAST
    Topological domaini83 – 9614ExtracellularBy similarityAdd
    BLAST
    Topological domaini119 – 13820CytoplasmicBy similarityAdd
    BLAST
    Topological domaini160 – 17920ExtracellularBy similarityAdd
    BLAST
    Topological domaini203 – 29189CytoplasmicBy similarityAdd
    BLAST
    Topological domaini315 – 32410ExtracellularBy similarity
    Topological domaini348 – 36518CytoplasmicBy similarityAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei23 – 4725Helical; Name=1By similarityAdd
    BLAST
    Transmembranei60 – 8223Helical; Name=2By similarityAdd
    BLAST
    Transmembranei97 – 11822Helical; Name=3By similarityAdd
    BLAST
    Transmembranei139 – 15921Helical; Name=4By similarityAdd
    BLAST
    Transmembranei180 – 20223Helical; Name=5By similarityAdd
    BLAST
    Transmembranei292 – 31423Helical; Name=6By similarityAdd
    BLAST
    Transmembranei325 – 34723Helical; Name=7By similarityAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni98 – 10710Agonist bindingBy similarity
    Regioni304 – 3085Agonist bindingBy similarity

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi119 – 1213DRY motif; important for ligand-induced conformation changesBy similarity
    Motifi340 – 3445NPxxY motif; important for ligand-induced conformation changes and signalingBy similarity

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG249628.
    HOGENOMiHOG000239242.
    HOVERGENiHBG106962.
    InParanoidiP28566.
    KOiK04153.
    OMAiPLSITYI.
    OrthoDBiEOG7NCV3Q.
    PhylomeDBiP28566.
    TreeFamiTF316350.

    Family and domain databases

    Gene3Di1.20.1070.10. 2 hits.
    InterProiIPR027425. 5HT1E_rcpt.
    IPR002231. 5HT_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    [Graphical view]
    PANTHERiPTHR24247:SF33. PTHR24247:SF33. 1 hit.
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR01101. 5HTRECEPTOR.
    PR00237. GPCRRHODOPSN.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P28566-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNITNCTTEA SMAIRPKTIT EKMLICMTLV VITTLTTLLN LAVIMAIGTT    50
    KKLHQPANYL ICSLAVTDLL VAVLVMPLSI IYIVMDRWKL GYFLCEVWLS 100
    VDMTCCTCSI LHLCVIALDR YWAITNAIEY ARKRTAKRAA LMILTVWTIS 150
    IFISMPPLFW RSHRRLSPPP SQCTIQHDHV IYTIYSTLGA FYIPLTLILI 200
    LYYRIYHAAK SLYQKRGSSR HLSNRSTDSQ NSFASCKLTQ TFCVSDFSTS 250
    DPTTEFEKFH ASIRIPPFDN DLDHPGERQQ ISSTRERKAA RILGLILGAF 300
    ILSWLPFFIK ELIVGLSIYT VSSEVADFLT WLGYVNSLIN PLLYTSFNED 350
    FKLAFKKLIR CREHT 365
    Length:365
    Mass (Da):41,682
    Last modified:December 1, 1992 - v1
    Checksum:i4C31DD783A3F7483
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti208 – 2081A → T.
    Corresponds to variant rs3828741 [ dbSNP | Ensembl ].
    VAR_022061
    Natural varianti262 – 2621S → F.1 Publication
    Corresponds to variant rs6303 [ dbSNP | Ensembl ].
    VAR_014165

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M91467 mRNA. Translation: AAA58353.1.
    Z11166 Genomic DNA. Translation: CAA77558.1.
    M92826 Genomic DNA. Translation: AAA58355.1.
    AF498980 mRNA. Translation: AAM21127.1.
    AL157777 Genomic DNA. Translation: CAC10582.1.
    CH471051 Genomic DNA. Translation: EAW48616.1.
    CH471051 Genomic DNA. Translation: EAW48617.1.
    BC069751 mRNA. Translation: AAH69751.1.
    AB041373 Genomic DNA. Translation: BAA94458.1.
    CCDSiCCDS5006.1.
    PIRiS20579. A45260.
    RefSeqiNP_000856.1. NM_000865.2.
    UniGeneiHs.1611.

    Genome annotation databases

    EnsembliENST00000305344; ENSP00000307766; ENSG00000168830.
    GeneIDi3354.
    KEGGihsa:3354.
    UCSCiuc003pli.3. human.

    Polymorphism databases

    DMDMi112822.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M91467 mRNA. Translation: AAA58353.1 .
    Z11166 Genomic DNA. Translation: CAA77558.1 .
    M92826 Genomic DNA. Translation: AAA58355.1 .
    AF498980 mRNA. Translation: AAM21127.1 .
    AL157777 Genomic DNA. Translation: CAC10582.1 .
    CH471051 Genomic DNA. Translation: EAW48616.1 .
    CH471051 Genomic DNA. Translation: EAW48617.1 .
    BC069751 mRNA. Translation: AAH69751.1 .
    AB041373 Genomic DNA. Translation: BAA94458.1 .
    CCDSi CCDS5006.1.
    PIRi S20579. A45260.
    RefSeqi NP_000856.1. NM_000865.2.
    UniGenei Hs.1611.

    3D structure databases

    ProteinModelPortali P28566.
    SMRi P28566. Positions 22-360.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 109586. 2 interactions.
    STRINGi 9606.ENSP00000307766.

    Chemistry

    BindingDBi P28566.
    ChEMBLi CHEMBL2096904.
    DrugBanki DB00216. Eletriptan.
    GuidetoPHARMACOLOGYi 4.

    Protein family/group databases

    GPCRDBi Search...

    Polymorphism databases

    DMDMi 112822.

    Proteomic databases

    PaxDbi P28566.
    PRIDEi P28566.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000305344 ; ENSP00000307766 ; ENSG00000168830 .
    GeneIDi 3354.
    KEGGi hsa:3354.
    UCSCi uc003pli.3. human.

    Organism-specific databases

    CTDi 3354.
    GeneCardsi GC06P087647.
    HGNCi HGNC:5291. HTR1E.
    HPAi HPA004931.
    MIMi 182132. gene.
    neXtProti NX_P28566.
    PharmGKBi PA29552.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG249628.
    HOGENOMi HOG000239242.
    HOVERGENi HBG106962.
    InParanoidi P28566.
    KOi K04153.
    OMAi PLSITYI.
    OrthoDBi EOG7NCV3Q.
    PhylomeDBi P28566.
    TreeFami TF316350.

    Enzyme and pathway databases

    Reactomei REACT_17064. Serotonin receptors.
    REACT_19231. G alpha (i) signalling events.

    Miscellaneous databases

    GeneWikii 5-HT1E_receptor.
    GenomeRNAii 3354.
    NextBioi 13262.
    PROi P28566.
    SOURCEi Search...

    Gene expression databases

    Bgeei P28566.
    CleanExi HS_HTR1E.
    Genevestigatori P28566.

    Family and domain databases

    Gene3Di 1.20.1070.10. 2 hits.
    InterProi IPR027425. 5HT1E_rcpt.
    IPR002231. 5HT_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    [Graphical view ]
    PANTHERi PTHR24247:SF33. PTHR24247:SF33. 1 hit.
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR01101. 5HTRECEPTOR.
    PR00237. GPCRRHODOPSN.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of a serotonin receptor from human brain (5HT1E): a fifth 5HT1-like subtype."
      McAllister G., Charlesworth A., Snodin C., Beer M.S., Noble A.J., Middlemiss D.N., Iversen L.L., Whiting P.
      Proc. Natl. Acad. Sci. U.S.A. 89:5517-5521(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
      Tissue: Brain.
    2. "Molecular cloning of a human gene (S31) encoding a novel serotonin receptor mediating inhibition of adenylyl cyclase."
      Levy F.O., Gudermann T., Birnbaumer M., Kaumann A.J., Birnbaumer L.
      FEBS Lett. 296:201-206(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
    3. "Human gene S31 encodes the pharmacologically defined serotonin 5-hydroxytryptamine1E receptor."
      Zgombick J.M., Schechter L.E., Macchi M., Hartig P.R., Branchek T.A., Weinshank R.L.
      Mol. Pharmacol. 42:180-185(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
    4. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
      Puhl H.L. III, Ikeda S.R., Aronstam R.S.
      Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    5. "The DNA sequence and analysis of human chromosome 6."
      Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
      , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
      Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    8. "Human-specific amino acid changes found in 103 protein-coding genes."
      Kitano T., Liu Y.-H., Ueda S., Saitou N.
      Mol. Biol. Evol. 21:936-944(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-363.
    9. "Molecular cloning and pharmacological characterization of the guinea pig 5-HT1E receptor."
      Bai F., Yin T., Johnstone E.M., Su C., Varga G., Little S.P., Nelson D.L.
      Eur. J. Pharmacol. 484:127-139(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    10. Cited for: REVIEW.
    11. "Toward selective drug development for the human 5-hydroxytryptamine 1E receptor: a comparison of 5-hydroxytryptamine 1E and 1F receptor structure-affinity relationships."
      Klein M.T., Dukat M., Glennon R.A., Teitler M.
      J. Pharmacol. Exp. Ther. 337:860-867(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    12. "Serotonin receptors - from molecular biology to clinical applications."
      Pytliak M., Vargova V., Mechirova V., Felsoci M.
      Physiol. Res. 60:15-25(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.
    13. Cited for: VARIANT PHE-262.

    Entry informationi

    Entry namei5HT1E_HUMAN
    AccessioniPrimary (citable) accession number: P28566
    Secondary accession number(s): E1P503, Q9P1Y1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 139 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. Human chromosome 6
      Human chromosome 6: entries, gene names and cross-references to MIM
    3. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    4. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    5. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3