Reviewed, UniProtKB/Swiss-Prot P28566 (5HT1E_HUMAN)

Names and origin

Protein names

Recommended name:
    5-hydroxytryptamine receptor 1E
      Short name=5-HT-1E
      Short name=5-HT1E
Alternative name(s):
    Serotonin receptor 1E
    S31

Gene names

Name:

HTR1E

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	G-protein signaling, coupled to cyclic nucleotide second messenger Ref.2 Traceable author statement. Source: ProtInc synaptic transmission Ref.1 Traceable author statement. Source: ProtInc
Cellular component	integral to plasma membrane Ref.2 Traceable author statement. Source: ProtInc
Molecular function	protein binding Inferred from physical interaction. Source: IntAct serotonin binding Ref.1 Inferred from direct assay. Source: MGI serotonin receptor activity Ref.1 Inferred from direct assay. Source: MGI
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PSMC3	P17980	1	EBI-1043151,EBI-359720
PSMC4	P43686	1	EBI-1043151,EBI-743997

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 365	365	5-hydroxytryptamine receptor 1E		PRO_0000068933
Regions
	Topological domain	1 – 22	22	Extracellular By similarity
	Transmembrane	23 – 47	25	1 By similarity
	Topological domain	48 – 59	12	Cytoplasmic By similarity
	Transmembrane	60 – 81	22	2 By similarity
	Topological domain	82 – 96	15	Extracellular By similarity
	Transmembrane	97 – 118	22	3 By similarity
	Topological domain	119 – 138	20	Cytoplasmic By similarity
	Transmembrane	139 – 159	21	4 By similarity
	Topological domain	160 – 179	20	Extracellular By similarity
	Transmembrane	180 – 201	22	5 By similarity
	Topological domain	202 – 291	90	Cytoplasmic By similarity
	Transmembrane	292 – 314	23	6 By similarity
	Topological domain	315 – 324	10	Extracellular By similarity
	Transmembrane	325 – 347	23	7 By similarity
	Topological domain	348 – 365	18	Cytoplasmic By similarity
Amino acid modifications
	Glycosylation	2	1	N-linked (GlcNAc...) Potential
	Glycosylation	5	1	N-linked (GlcNAc...) Potential
	Disulfide bond	95 ↔ 173		By similarity
Natural variations
	Natural variant	208	1	A → T: dbSNP rs3828741.		VAR_022061
	Natural variant	262	1	S → F: dbSNP rs6303. Ref.8		VAR_014165

Sequences

	Sequence		Length	Mass (Da)
	P28566-1 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 4C31DD783A3F7483	FASTA	365	41,682
	10 20 30 40 50 60 MNITNCTTEA SMAIRPKTIT EKMLICMTLV VITTLTTLLN LAVIMAIGTT KKLHQPANYL 70 80 90 100 110 120 ICSLAVTDLL VAVLVMPLSI IYIVMDRWKL GYFLCEVWLS VDMTCCTCSI LHLCVIALDR 130 140 150 160 170 180 YWAITNAIEY ARKRTAKRAA LMILTVWTIS IFISMPPLFW RSHRRLSPPP SQCTIQHDHV 190 200 210 220 230 240 IYTIYSTLGA FYIPLTLILI LYYRIYHAAK SLYQKRGSSR HLSNRSTDSQ NSFASCKLTQ 250 260 270 280 290 300 TFCVSDFSTS DPTTEFEKFH ASIRIPPFDN DLDHPGERQQ ISSTRERKAA RILGLILGAF 310 320 330 340 350 360 ILSWLPFFIK ELIVGLSIYT VSSEVADFLT WLGYVNSLIN PLLYTSFNED FKLAFKKLIR CREHT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of a serotonin receptor from human brain (5HT1E): a fifth 5HT1-like subtype." McAllister G., Charlesworth A., Snodin C., Beer M.S., Noble A.J., Middlemiss D.N., Iversen L.L., Whiting P. Proc. Natl. Acad. Sci. U.S.A. 89:5517-5521(1992) [PubMed: 1608964] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Molecular cloning of a human gene (S31) encoding a novel serotonin receptor mediating inhibition of adenylyl cyclase." Levy F.O., Gudermann T., Birnbaumer M., Kaumann A.J., Birnbaumer L. FEBS Lett. 296:201-206(1992) [PubMed: 1733778] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Human gene S31 encodes the pharmacologically defined serotonin 5-hydroxytryptamine1E receptor." Zgombick J.M., Schechter L.E., Macchi M., Hartig P.R., Branchek T.A., Weinshank R.L. Mol. Pharmacol. 42:180-185(1992) [PubMed: 1513320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed: 14574404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]	"Human-specific amino acid changes found in 103 protein-coding genes." Kitano T., Liu Y.-H., Ueda S., Saitou N. Mol. Biol. Evol. 21:936-944(2004) [PubMed: 15014171] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-363.
[8]	"Characterization of single-nucleotide polymorphisms in coding regions of human genes." Cargill M., Altshuler D., Ireland J., Sklar P., Ardlie K., Patil N., Shaw N., Lane C.R., Lim E.P., Kalyanaraman N., Nemesh J., Ziaugra L., Friedland L., Rolfe A., Warrington J., Lipshutz R., Daley G.Q., Lander E.S. Nat. Genet. 22:231-238(1999) [PubMed: 10391209] [Abstract] Cited for: VARIANT PHE-262.
[9]	Erratum Cargill M., Altshuler D., Ireland J., Sklar P., Ardlie K., Patil N., Shaw N., Lane C.R., Lim E.P., Kalyanaraman N., Nemesh J., Ziaugra L., Friedland L., Rolfe A., Warrington J., Lipshutz R., Daley G.Q., Lander E.S. Nat. Genet. 23:373-373(1999)
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M91467 mRNA. Translation: AAA58353.1. Z11166 Genomic DNA. Translation: CAA77558.1. M92826 Genomic DNA. Translation: AAA58355.1. AF498980 mRNA. Translation: AAM21127.1. AL157777 Genomic DNA. Translation: CAC10582.1. BC069751 mRNA. Translation: AAH69751.1. AB041373 Genomic DNA. Translation: BAA94458.1.
IPI	IPI00003932.
PIR	A45260. S20579.
RefSeq	NP_000856.1.
UniGene	Hs.1611
3D structure databases
HSSP	HSSP built from PDB template 1HLL based on UniProtKB P08913.
ModBase	Search...
Protein-protein interaction databases
IntAct	P28566. 2 interactions.
Protein family/group databases
GPCRDB	Search...
Proteomic databases
PRIDE	P28566.
Genome annotation databases
Ensembl	ENSG00000168830. Homo sapiens. [Contig view]
GeneID	3354.
KEGG	hsa:3354.
UCSC	uc003plg.1. human.
Organism-specific databases
GeneCards	GC06P087704.
H-InvDB	HIX0032800.
HGNC	HGNC:5291. HTR1E.
HPA	HPA004931.
MIM	182132. gene.
PharmGKB	PA29552.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	P28566.
HOVERGEN	P28566.
OMA	P28566. FNEDFKL.
Enzyme and pathway databases
Reactome	REACT_14797. Signaling by GPCR.
Gene expression databases
ArrayExpress	P28566.
Bgee	P28566.
CleanEx	HS_HTR1E.
GermOnline	ENSG00000168830. Homo sapiens.
Family and domain databases
InterPro	IPR000450. 5HT1F_rcpt. IPR002231. 5HT_rcpt. IPR000276. 7TM_GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. [Graphical view]
PANTHER	PTHR19266:SF65. 5HT1F_rcpt. 1 hit.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR01101. 5HTRECEPTOR. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
DrugBank	DB00216. Eletriptan.
NextBio	13262.
SOURCE	Search...

Entry information

Entry name

5HT1E_HUMAN

Accession

Primary (citable) accession number: P28566
Secondary accession number(s): Q9P1Y1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	July 7, 2009
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)