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Protein

5-hydroxytryptamine receptor 1D

Gene

Htr1d

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various alkaloids and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity. Regulates the release of 5-hydroxytryptamine in the brain, and thereby affects neural activity. May also play a role in regulating the release of other neurotransmitters. May play a role in vasoconstriction.2 Publications

GO - Molecular functioni

  1. serotonin binding Source: Ensembl
  2. serotonin receptor activity Source: UniProtKB

GO - Biological processi

  1. adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway Source: UniProtKB
  2. intestine smooth muscle contraction Source: Ensembl
  3. regulation of behavior Source: InterPro
  4. regulation of locomotion Source: InterPro
  5. response to toxic substance Source: Ensembl
  6. serotonin receptor signaling pathway Source: GOC
  7. synaptic transmission Source: InterPro
  8. vasoconstriction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_339234. G alpha (i) signalling events.
REACT_343696. Serotonin receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
5-hydroxytryptamine receptor 1D
Short name:
5-HT-1D
Short name:
5-HT1D
Alternative name(s):
Serotonin receptor 1D
Gene namesi
Name:Htr1d
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi2847. Htr1d.

Subcellular locationi

  1. Cell membrane 2 Publications; Multi-pass membrane protein 2 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3535ExtracellularBy similarityAdd
BLAST
Transmembranei36 – 6126Helical; Name=1By similarityAdd
BLAST
Topological domaini62 – 7211CytoplasmicBy similarityAdd
BLAST
Transmembranei73 – 9523Helical; Name=2By similarityAdd
BLAST
Topological domaini96 – 10914ExtracellularBy similarityAdd
BLAST
Transmembranei110 – 13122Helical; Name=3By similarityAdd
BLAST
Topological domaini132 – 15120CytoplasmicBy similarityAdd
BLAST
Transmembranei152 – 17322Helical; Name=4By similarityAdd
BLAST
Topological domaini174 – 19118ExtracellularBy similarityAdd
BLAST
Transmembranei192 – 21423Helical; Name=5By similarityAdd
BLAST
Topological domaini215 – 29985CytoplasmicBy similarityAdd
BLAST
Transmembranei300 – 32324Helical; Name=6By similarityAdd
BLAST
Topological domaini324 – 3329ExtracellularBy similarity
Transmembranei333 – 35725Helical; Name=7By similarityAdd
BLAST
Topological domaini358 – 37417CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  1. integral component of plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3743745-hydroxytryptamine receptor 1DPRO_0000068931Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi5 – 51N-linked (GlcNAc...)Sequence Analysis
Glycosylationi17 – 171N-linked (GlcNAc...)Sequence Analysis
Glycosylationi21 – 211N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi108 ↔ 185PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP28565.
PRIDEiP28565.

PTM databases

PhosphoSiteiP28565.

Expressioni

Tissue specificityi

Detected in dorsal raphe.1 Publication

Gene expression databases

GenevestigatoriP28565.

Interactioni

Subunit structurei

Homodimer. Heterodimer with HTR1B (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000016047.

Structurei

3D structure databases

ProteinModelPortaliP28565.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni111 – 12010Agonist bindingBy similarity
Regioni311 – 3155Agonist bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi132 – 1343DRY motif; important for ligand-induced conformation changesBy similarity
Motifi349 – 3535NPxxY motif; important for ligand-induced conformation changes and signalingBy similarity

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG249628.
GeneTreeiENSGT00760000118795.
HOGENOMiHOG000239242.
HOVERGENiHBG106962.
InParanoidiP28565.
KOiK04153.
OMAiCRDSCWI.
OrthoDBiEOG7NCV3Q.
PhylomeDBiP28565.
TreeFamiTF316350.

Family and domain databases

InterProiIPR000505. 5HT1D_rcpt.
IPR002231. 5HT_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24247:SF17. PTHR24247:SF17. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00514. 5HT1DRECEPTR.
PR01101. 5HTRECEPTOR.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28565-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLPNQSLEG LPQEASNRSL NATGAWDPEV LQALRISLVV VLSIITLATV
60 70 80 90 100
LSNAFVLTTI LLTKKLHTPA NYLIGSLATT DLLVSILVMP ISIAYTTTRT
110 120 130 140 150
WNFGQILCDI WVSSDITCCT ASILHLCVIA LDRYWAITDA LEYSKRRTAG
160 170 180 190 200
HAAAMIAAVW AISICISIPP LFWRQATAHE EMSDCLVNTS QISYTIYSTC
210 220 230 240 250
GAFYIPSILL IILYGRIYVA ARSRILNPPS LYGKRFTTAQ LITGSAGSSL
260 270 280 290 300
CSLNPSLHES HTHTVGSPLF FNQVKIKLAD SILERKRISA ARERKATKTL
310 320 330 340 350
GIILGAFIIC WLPFFVVSLV LPICRDSCWI HPALFDFFTW LGYLNSLINP
360 370
VIYTVFNEDF RQAFQRVVHF RKAS
Length:374
Mass (Da):41,540
Last modified:December 1, 1992 - v1
Checksum:i345CBD528317DA21
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M89953 Genomic DNA. Translation: AAA40614.1.
PIRiI77467.
RefSeqiNP_036984.1. NM_012852.1.
XP_008762430.1. XM_008764208.1.
UniGeneiRn.34834.

Genome annotation databases

EnsembliENSRNOT00000016046; ENSRNOP00000016047; ENSRNOG00000012038.
GeneIDi25323.
KEGGirno:25323.
UCSCiRGD:2847. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M89953 Genomic DNA. Translation: AAA40614.1.
PIRiI77467.
RefSeqiNP_036984.1. NM_012852.1.
XP_008762430.1. XM_008764208.1.
UniGeneiRn.34834.

3D structure databases

ProteinModelPortaliP28565.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000016047.

Chemistry

BindingDBiP28565.
ChEMBLiCHEMBL3301381.
GuidetoPHARMACOLOGYi3.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiP28565.

Proteomic databases

PaxDbiP28565.
PRIDEiP28565.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000016046; ENSRNOP00000016047; ENSRNOG00000012038.
GeneIDi25323.
KEGGirno:25323.
UCSCiRGD:2847. rat.

Organism-specific databases

CTDi3352.
RGDi2847. Htr1d.

Phylogenomic databases

eggNOGiNOG249628.
GeneTreeiENSGT00760000118795.
HOGENOMiHOG000239242.
HOVERGENiHBG106962.
InParanoidiP28565.
KOiK04153.
OMAiCRDSCWI.
OrthoDBiEOG7NCV3Q.
PhylomeDBiP28565.
TreeFamiTF316350.

Enzyme and pathway databases

ReactomeiREACT_339234. G alpha (i) signalling events.
REACT_343696. Serotonin receptors.

Miscellaneous databases

NextBioi606173.
PROiP28565.

Gene expression databases

GenevestigatoriP28565.

Family and domain databases

InterProiIPR000505. 5HT1D_rcpt.
IPR002231. 5HT_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24247:SF17. PTHR24247:SF17. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00514. 5HT1DRECEPTR.
PR01101. 5HTRECEPTOR.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Distinct 5-HT1B and 5-HT1D serotonin receptors in rat: structural and pharmacological comparison of the two cloned receptors."
    Hamblin M.W., McGuffin R.W., Metcalf M.A., Dorsa D.M., Merchant K.M.
    Mol. Cell. Neurosci. 3:578-587(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  2. "Sequence and functional analysis of cloned guinea pig and rat serotonin 5-HT1D receptors: common pharmacological features within the 5-HT1D receptor subfamily."
    Wurch T., Palmier C., Colpaert F.C., Pauwels P.J.
    J. Neurochem. 68:410-418(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.

Entry informationi

Entry namei5HT1D_RAT
AccessioniPrimary (citable) accession number: P28565
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: April 1, 2015
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.