Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P28564

- 5HT1B_RAT

UniProt

P28564 - 5HT1B_RAT

Protein

5-hydroxytryptamine receptor 1B

Gene

Htr1b

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 1 (01 Dec 1992)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various alkaloids and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity. Arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Regulates the release of 5-hydroxytryptamine, dopamine and acetylcholine in the brain, and thereby affects neural activity, nociceptive processing, pain perception, mood and behavior. Besides, plays a role in vasoconstriction of cerebral arteries.2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei351 – 3511Important for species-specific agonist sensitivityBy similarity

    GO - Molecular functioni

    1. drug binding Source: RGD
    2. serotonin binding Source: RGD
    3. serotonin receptor activity Source: RGD

    GO - Biological processi

    1. adenylate cyclase-inhibiting serotonin receptor signaling pathway Source: UniProtKB
    2. bone remodeling Source: Ensembl
    3. cellular response to alkaloid Source: UniProtKB
    4. cellular response to drug Source: UniProtKB
    5. cellular response to temperature stimulus Source: Ensembl
    6. drinking behavior Source: RGD
    7. feeding behavior Source: RGD
    8. G-protein coupled receptor internalization Source: Ensembl
    9. negative regulation of cAMP biosynthetic process Source: Ensembl
    10. negative regulation of serotonin secretion Source: UniProtKB
    11. negative regulation of synaptic transmission, GABAergic Source: RGD
    12. negative regulation of synaptic transmission, glutamatergic Source: RGD
    13. protein kinase C-activating G-protein coupled receptor signaling pathway Source: Ensembl
    14. regulation of behavior Source: InterPro
    15. regulation of dopamine secretion Source: RGD
    16. response to cocaine Source: RGD
    17. response to ethanol Source: RGD
    18. response to mineralocorticoid Source: RGD
    19. synaptic transmission Source: RGD
    20. vasoconstriction Source: InterPro

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Keywords - Biological processi

    Behavior

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    5-hydroxytryptamine receptor 1B
    Short name:
    5-HT-1B
    Short name:
    5-HT1B
    Alternative name(s):
    Serotonin receptor 1B
    Gene namesi
    Name:Htr1b
    Synonyms:5ht1b
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 8

    Organism-specific databases

    RGDi2846. Htr1b.

    Subcellular locationi

    Cell membrane 2 Publications; Multi-pass membrane protein 2 Publications

    GO - Cellular componenti

    1. cytoplasm Source: Ensembl
    2. integral component of plasma membrane Source: UniProtKB
    3. plasma membrane Source: RGD

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 3863865-hydroxytryptamine receptor 1BPRO_0000068921Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi24 – 241N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi28 – 281N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi118 ↔ 195PROSITE-ProRule annotation
    Lipidationi384 – 3841S-palmitoyl cysteineSequence Analysis

    Post-translational modificationi

    Phosphorylated.By similarity
    Palmitoylated.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

    Proteomic databases

    PRIDEiP28564.

    PTM databases

    PhosphoSiteiP28564.

    Expressioni

    Gene expression databases

    GenevestigatoriP28564.

    Interactioni

    Subunit structurei

    Homodimer. Heterodimer with HTR1D By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000017411.

    Structurei

    3D structure databases

    ProteinModelPortaliP28564.
    SMRiP28564. Positions 130-161.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 4545ExtracellularBy similarityAdd
    BLAST
    Topological domaini72 – 809CytoplasmicBy similarity
    Topological domaini107 – 11913ExtracellularBy similarityAdd
    BLAST
    Topological domaini142 – 16120CytoplasmicBy similarityAdd
    BLAST
    Topological domaini184 – 20118ExtracellularBy similarityAdd
    BLAST
    Topological domaini225 – 31187CytoplasmicBy similarityAdd
    BLAST
    Topological domaini333 – 34513ExtracellularBy similarityAdd
    BLAST
    Topological domaini368 – 38619CytoplasmicBy similarityAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei46 – 7126Helical; Name=1By similarityAdd
    BLAST
    Transmembranei81 – 10626Helical; Name=2By similarityAdd
    BLAST
    Transmembranei120 – 14122Helical; Name=3By similarityAdd
    BLAST
    Transmembranei162 – 18322Helical; Name=4By similarityAdd
    BLAST
    Transmembranei202 – 22423Helical; Name=5By similarityAdd
    BLAST
    Transmembranei312 – 33221Helical; Name=6By similarityAdd
    BLAST
    Transmembranei346 – 36722Helical; Name=7By similarityAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni121 – 13010Agonist bindingBy similarity
    Regioni323 – 3275Agonist bindingBy similarity

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi142 – 1443DRY motif; important for ligand-induced conformation changes and signalingBy similarity
    Motifi361 – 3655NPxxY motif; important for ligand-induced conformation changes and signalingBy similarity

    Domaini

    Ligands are bound in a hydrophobic pocket formed by the transmembrane helices.By similarity

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG249628.
    GeneTreeiENSGT00750000117301.
    HOGENOMiHOG000239242.
    HOVERGENiHBG106962.
    InParanoidiP28564.
    KOiK04153.
    OMAiIALPWKV.
    OrthoDBiEOG7NCV3Q.
    PhylomeDBiP28564.
    TreeFamiTF316350.

    Family and domain databases

    Gene3Di1.20.1070.10. 2 hits.
    InterProiIPR002147. 5HT1B_rcpt.
    IPR002231. 5HT_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    [Graphical view]
    PANTHERiPTHR24247:SF16. PTHR24247:SF16. 1 hit.
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR00513. 5HT1BRECEPTR.
    PR01101. 5HTRECEPTOR.
    PR00237. GPCRRHODOPSN.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P28564-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEEQGIQCAP PPPATSQTGV PLANLSHNCS ADDYIYQDSI ALPWKVLLVA    50
    LLALITLATT LSNAFVIATV YRTRKLHTPA NYLIASLAVT DLLVSILVMP 100
    ISTMYTVTGR WTLGQVVCDF WLSSDITCCT ASIMHLCVIA LDRYWAITDA 150
    VDYSAKRTPK RAAIMIVLVW VFSISISLPP FFWRQAKAEE EVLDCFVNTD 200
    HVLYTVYSTV GAFYLPTLLL IALYGRIYVE ARSRILKQTP NKTGKRLTRA 250
    QLITDSPGST SSVTSINSRV PEVPSESGSP VYVNQVKVRV SDALLEKKKL 300
    MAARERKATK TLGIILGAFI VCWLPFFIIS LVMPICKDAC WFHMAIFDFF 350
    NWLGYLNSLI NPIIYTMSNE DFKQAFHKLI RFKCTG 386
    Length:386
    Mass (Da):43,163
    Last modified:December 1, 1992 - v1
    Checksum:i4F4E9AC1C9AED214
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M89954 Genomic DNA. Translation: AAA40613.1.
    X62944 mRNA. Translation: CAA44716.1.
    PIRiS18637.
    RefSeqiNP_071561.1. NM_022225.1.
    UniGeneiRn.138109.

    Genome annotation databases

    EnsembliENSRNOT00000017411; ENSRNOP00000017411; ENSRNOG00000013042.
    GeneIDi25075.
    KEGGirno:25075.
    UCSCiRGD:2846. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M89954 Genomic DNA. Translation: AAA40613.1 .
    X62944 mRNA. Translation: CAA44716.1 .
    PIRi S18637.
    RefSeqi NP_071561.1. NM_022225.1.
    UniGenei Rn.138109.

    3D structure databases

    ProteinModelPortali P28564.
    SMRi P28564. Positions 130-161.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000017411.

    Chemistry

    BindingDBi P28564.
    ChEMBLi CHEMBL2095159.
    GuidetoPHARMACOLOGYi 2.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei P28564.

    Proteomic databases

    PRIDEi P28564.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000017411 ; ENSRNOP00000017411 ; ENSRNOG00000013042 .
    GeneIDi 25075.
    KEGGi rno:25075.
    UCSCi RGD:2846. rat.

    Organism-specific databases

    CTDi 3351.
    RGDi 2846. Htr1b.

    Phylogenomic databases

    eggNOGi NOG249628.
    GeneTreei ENSGT00750000117301.
    HOGENOMi HOG000239242.
    HOVERGENi HBG106962.
    InParanoidi P28564.
    KOi K04153.
    OMAi IALPWKV.
    OrthoDBi EOG7NCV3Q.
    PhylomeDBi P28564.
    TreeFami TF316350.

    Miscellaneous databases

    NextBioi 605318.
    PROi P28564.

    Gene expression databases

    Genevestigatori P28564.

    Family and domain databases

    Gene3Di 1.20.1070.10. 2 hits.
    InterProi IPR002147. 5HT1B_rcpt.
    IPR002231. 5HT_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    [Graphical view ]
    PANTHERi PTHR24247:SF16. PTHR24247:SF16. 1 hit.
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00513. 5HT1BRECEPTR.
    PR01101. 5HTRECEPTOR.
    PR00237. GPCRRHODOPSN.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Distinct 5-HT1B and 5-HT1D serotonin receptors in rat: structural and pharmacological comparison of the two cloned receptors."
      Hamblin M.W., McGuffin R.W., Metcalf M.A., Dorsa D.M., Merchant K.M.
      Mol. Cell. Neurosci. 3:578-587(1992)
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
    2. "Molecular cloning and characterization of a rat brain cDNA encoding a 5-hydroxytryptamine1B receptor."
      Voigt M.M., Laurie D.J., Seeburg P.H., Bach A.
      EMBO J. 10:4017-4023(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
      Tissue: Brain.

    Entry informationi

    Entry namei5HT1B_RAT
    AccessioniPrimary (citable) accession number: P28564
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    A residue in the 7th transmembrane region ('Thr-355' in human, Asn-351 in mouse and rat) is important for species-specific sensitivity to various agonists.By similarity

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3