P28564 (5HT1B_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-hydroxytryptamine receptor 1B
Short name=5-HT-1B
Short name=5-HT1B

Alternative name(s):
Serotonin receptor 1B

Gene names

Name:	Htr1b
Synonyms:	5ht1b

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	386 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Post-translational modification	Phosphorylated By similarity. Palmitoylated By similarity.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G-protein coupled receptor internalization Inferred from electronic annotation. Source: Compara bone remodeling Inferred from electronic annotation. Source: Compara cellular response to temperature stimulus Inferred from electronic annotation. Source: Compara drinking behavior Inferred from mutant phenotype PubMed 16839853. Source: RGD negative regulation of cAMP biosynthetic process Inferred from electronic annotation. Source: Compara negative regulation of synaptic transmission, GABAergic Inferred from mutant phenotype PubMed 17229091. Source: RGD negative regulation of synaptic transmission, glutamatergic Inferred from mutant phenotype PubMed 17392733. Source: RGD protein kinase C-activating G-protein coupled receptor signaling pathway Inferred from electronic annotation. Source: Compara regulation of dopamine secretion Inferred from mutant phenotype PubMed 16885935. Source: RGD response to cocaine Inferred from direct assay PubMed 17509084. Source: RGD response to drug Inferred from electronic annotation. Source: Compara response to ethanol Inferred from mutant phenotype PubMed 16212943. Source: RGD response to mineralocorticoid stimulus Inferred from expression pattern PubMed 11882579. Source: RGD synaptic transmission Inferred from direct assay PubMed 15328035. Source: RGD
Cellular_component	cytoplasm Inferred from electronic annotation. Source: Compara integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay PubMed 15328035. Source: RGD
Molecular_function	drug binding Inferred from direct assay PubMed 16546225. Source: RGD serotonin binding Inferred from direct assay PubMed 2947981. Source: RGD serotonin receptor activity Inferred from direct assay PubMed 15328035. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 386	386	5-hydroxytryptamine receptor 1B		PRO_0000068921
Regions
	Topological domain	1 – 47	47	Extracellular By similarity
	Transmembrane	48 – 71	24	Helical; Name=1; By similarity
	Topological domain	72 – 82	11	Cytoplasmic By similarity
	Transmembrane	83 – 109	27	Helical; Name=2; By similarity
	Topological domain	110 – 119	10	Extracellular By similarity
	Transmembrane	120 – 141	22	Helical; Name=3; By similarity
	Topological domain	142 – 163	22	Cytoplasmic By similarity
	Transmembrane	164 – 183	20	Helical; Name=4; By similarity
	Topological domain	184 – 204	21	Extracellular By similarity
	Transmembrane	205 – 224	20	Helical; Name=5; By similarity
	Topological domain	225 – 310	86	Cytoplasmic By similarity
	Transmembrane	311 – 332	22	Helical; Name=6; By similarity
	Topological domain	333 – 346	14	Extracellular By similarity
	Transmembrane	347 – 369	23	Helical; Name=7; By similarity
	Topological domain	370 – 386	17	Cytoplasmic By similarity
Amino acid modifications
	Lipidation	384	1	S-palmitoyl cysteine Potential
	Glycosylation	24	1	N-linked (GlcNAc...) Potential
	Glycosylation	28	1	N-linked (GlcNAc...) Potential
	Disulfide bond	118 ↔ 195		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P28564 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 4F4E9AC1C9AED214	FASTA	386	43,163
	10 20 30 40 50 60 MEEQGIQCAP PPPATSQTGV PLANLSHNCS ADDYIYQDSI ALPWKVLLVA LLALITLATT 70 80 90 100 110 120 LSNAFVIATV YRTRKLHTPA NYLIASLAVT DLLVSILVMP ISTMYTVTGR WTLGQVVCDF 130 140 150 160 170 180 WLSSDITCCT ASIMHLCVIA LDRYWAITDA VDYSAKRTPK RAAIMIVLVW VFSISISLPP 190 200 210 220 230 240 FFWRQAKAEE EVLDCFVNTD HVLYTVYSTV GAFYLPTLLL IALYGRIYVE ARSRILKQTP 250 260 270 280 290 300 NKTGKRLTRA QLITDSPGST SSVTSINSRV PEVPSESGSP VYVNQVKVRV SDALLEKKKL 310 320 330 340 350 360 MAARERKATK TLGIILGAFI VCWLPFFIIS LVMPICKDAC WFHMAIFDFF NWLGYLNSLI 370 380 NPIIYTMSNE DFKQAFHKLI RFKCTG

References

[1]	"Distinct 5-HT1B and 5-HT1D serotonin receptors in rat: structural and pharmacological comparison of the two cloned receptors." Hamblin M.W., McGuffin R.W., Metcalf M.A., Dorsa D.M., Merchant K.M. Mol. Cell. Neurosci. 3:578-587(1992) Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Molecular cloning and characterization of a rat brain cDNA encoding a 5-hydroxytryptamine1B receptor." Voigt M.M., Laurie D.J., Seeburg P.H., Bach A. EMBO J. 10:4017-4023(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M89954 Genomic DNA. Translation: AAA40613.1. X62944 mRNA. Translation: CAA44716.1.
IPI	IPI00201813.
PIR	S18637.
RefSeq	NP_071561.1. NM_022225.1.
UniGene	Rn.138109.
3D structure databases
ProteinModelPortal	P28564.
SMR	P28564. Positions 130-161.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000017411.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P28564.
Proteomic databases
PRIDE	P28564.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000017411; ENSRNOP00000017411; ENSRNOG00000013042.
GeneID	25075.
KEGG	rno:25075.
UCSC	RGD:2846. rat.
Organism-specific databases
CTD	3351.
RGD	2846. Htr1b.
Phylogenomic databases
eggNOG	NOG249628.
GeneTree	ENSGT00650000092921.
HOGENOM	HOG000239242.
HOVERGEN	HBG106962.
InParanoid	P28564.
KO	K04153.
OMA	IALPWKV.
OrthoDB	EOG4R7VB6.
Gene expression databases
Genevestigator	P28564.
GermOnline	ENSRNOG00000013042. Rattus norvegicus.
Family and domain databases
InterPro	IPR002147. 5HT1B_rcpt. IPR002231. 5HT_rcpt. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00513. 5HT1BRECEPTR. PR01101. 5HTRECEPTOR. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P28564.
ChEMBL	CHEMBL3459.
NextBio	605318.

Entry information

Entry name

5HT1B_RAT

Accession

Primary (citable) accession number: P28564

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	April 3, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program