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Protein

5-hydroxytryptamine receptor 1B

Gene

Htr1b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various alkaloids and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity. Arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Regulates the release of 5-hydroxytryptamine, dopamine and acetylcholine in the brain, and thereby affects neural activity, nociceptive processing, pain perception, mood and behavior. Besides, plays a role in vasoconstriction of cerebral arteries.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei351 – 3511Important for species-specific agonist sensitivityBy similarity

GO - Molecular functioni

  1. drug binding Source: RGD
  2. serotonin binding Source: RGD
  3. serotonin receptor activity Source: RGD

GO - Biological processi

  1. adenylate cyclase-inhibiting serotonin receptor signaling pathway Source: UniProtKB
  2. bone remodeling Source: Ensembl
  3. cellular response to alkaloid Source: UniProtKB
  4. cellular response to drug Source: UniProtKB
  5. cellular response to temperature stimulus Source: Ensembl
  6. drinking behavior Source: RGD
  7. feeding behavior Source: RGD
  8. G-protein coupled receptor internalization Source: Ensembl
  9. negative regulation of cAMP biosynthetic process Source: Ensembl
  10. negative regulation of serotonin secretion Source: UniProtKB
  11. negative regulation of synaptic transmission, GABAergic Source: RGD
  12. negative regulation of synaptic transmission, glutamatergic Source: RGD
  13. protein kinase C-activating G-protein coupled receptor signaling pathway Source: Ensembl
  14. regulation of behavior Source: InterPro
  15. regulation of dopamine secretion Source: RGD
  16. response to cocaine Source: RGD
  17. response to ethanol Source: RGD
  18. response to mineralocorticoid Source: RGD
  19. synaptic transmission Source: RGD
  20. vasoconstriction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Behavior

Enzyme and pathway databases

ReactomeiREACT_339234. G alpha (i) signalling events.
REACT_343696. Serotonin receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
5-hydroxytryptamine receptor 1B
Short name:
5-HT-1B
Short name:
5-HT1B
Alternative name(s):
Serotonin receptor 1B
Gene namesi
Name:Htr1b
Synonyms:5ht1b
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi2846. Htr1b.

Subcellular locationi

  1. Cell membrane 2 Publications; Multi-pass membrane protein 2 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4545ExtracellularBy similarityAdd
BLAST
Transmembranei46 – 7126Helical; Name=1By similarityAdd
BLAST
Topological domaini72 – 809CytoplasmicBy similarity
Transmembranei81 – 10626Helical; Name=2By similarityAdd
BLAST
Topological domaini107 – 11913ExtracellularBy similarityAdd
BLAST
Transmembranei120 – 14122Helical; Name=3By similarityAdd
BLAST
Topological domaini142 – 16120CytoplasmicBy similarityAdd
BLAST
Transmembranei162 – 18322Helical; Name=4By similarityAdd
BLAST
Topological domaini184 – 20118ExtracellularBy similarityAdd
BLAST
Transmembranei202 – 22423Helical; Name=5By similarityAdd
BLAST
Topological domaini225 – 31187CytoplasmicBy similarityAdd
BLAST
Transmembranei312 – 33221Helical; Name=6By similarityAdd
BLAST
Topological domaini333 – 34513ExtracellularBy similarityAdd
BLAST
Transmembranei346 – 36722Helical; Name=7By similarityAdd
BLAST
Topological domaini368 – 38619CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  1. cytoplasm Source: Ensembl
  2. integral component of plasma membrane Source: UniProtKB
  3. plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3863865-hydroxytryptamine receptor 1BPRO_0000068921Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi24 – 241N-linked (GlcNAc...)Sequence Analysis
Glycosylationi28 – 281N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi118 ↔ 195PROSITE-ProRule annotation
Lipidationi384 – 3841S-palmitoyl cysteineSequence Analysis

Post-translational modificationi

Phosphorylated.By similarity
Palmitoylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PRIDEiP28564.

PTM databases

PhosphoSiteiP28564.

Expressioni

Gene expression databases

GenevestigatoriP28564.

Interactioni

Subunit structurei

Homodimer. Heterodimer with HTR1D (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017411.

Structurei

3D structure databases

ProteinModelPortaliP28564.
SMRiP28564. Positions 130-161.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni121 – 13010Agonist bindingBy similarity
Regioni323 – 3275Agonist bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi142 – 1443DRY motif; important for ligand-induced conformation changes and signalingBy similarity
Motifi361 – 3655NPxxY motif; important for ligand-induced conformation changes and signalingBy similarity

Domaini

Ligands are bound in a hydrophobic pocket formed by the transmembrane helices.By similarity

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG249628.
GeneTreeiENSGT00760000118795.
HOGENOMiHOG000239242.
HOVERGENiHBG106962.
InParanoidiP28564.
KOiK04153.
OMAiIALPWKV.
OrthoDBiEOG7NCV3Q.
PhylomeDBiP28564.
TreeFamiTF316350.

Family and domain databases

InterProiIPR002147. 5HT1B_rcpt.
IPR002231. 5HT_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24247:SF16. PTHR24247:SF16. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00513. 5HT1BRECEPTR.
PR01101. 5HTRECEPTOR.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28564-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEQGIQCAP PPPATSQTGV PLANLSHNCS ADDYIYQDSI ALPWKVLLVA
60 70 80 90 100
LLALITLATT LSNAFVIATV YRTRKLHTPA NYLIASLAVT DLLVSILVMP
110 120 130 140 150
ISTMYTVTGR WTLGQVVCDF WLSSDITCCT ASIMHLCVIA LDRYWAITDA
160 170 180 190 200
VDYSAKRTPK RAAIMIVLVW VFSISISLPP FFWRQAKAEE EVLDCFVNTD
210 220 230 240 250
HVLYTVYSTV GAFYLPTLLL IALYGRIYVE ARSRILKQTP NKTGKRLTRA
260 270 280 290 300
QLITDSPGST SSVTSINSRV PEVPSESGSP VYVNQVKVRV SDALLEKKKL
310 320 330 340 350
MAARERKATK TLGIILGAFI VCWLPFFIIS LVMPICKDAC WFHMAIFDFF
360 370 380
NWLGYLNSLI NPIIYTMSNE DFKQAFHKLI RFKCTG
Length:386
Mass (Da):43,163
Last modified:December 1, 1992 - v1
Checksum:i4F4E9AC1C9AED214
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M89954 Genomic DNA. Translation: AAA40613.1.
X62944 mRNA. Translation: CAA44716.1.
PIRiS18637.
RefSeqiNP_071561.1. NM_022225.1.
UniGeneiRn.138109.

Genome annotation databases

EnsembliENSRNOT00000017411; ENSRNOP00000017411; ENSRNOG00000013042.
GeneIDi25075.
KEGGirno:25075.
UCSCiRGD:2846. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M89954 Genomic DNA. Translation: AAA40613.1.
X62944 mRNA. Translation: CAA44716.1.
PIRiS18637.
RefSeqiNP_071561.1. NM_022225.1.
UniGeneiRn.138109.

3D structure databases

ProteinModelPortaliP28564.
SMRiP28564. Positions 130-161.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017411.

Chemistry

BindingDBiP28564.
ChEMBLiCHEMBL3301381.
GuidetoPHARMACOLOGYi2.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiP28564.

Proteomic databases

PRIDEiP28564.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000017411; ENSRNOP00000017411; ENSRNOG00000013042.
GeneIDi25075.
KEGGirno:25075.
UCSCiRGD:2846. rat.

Organism-specific databases

CTDi3351.
RGDi2846. Htr1b.

Phylogenomic databases

eggNOGiNOG249628.
GeneTreeiENSGT00760000118795.
HOGENOMiHOG000239242.
HOVERGENiHBG106962.
InParanoidiP28564.
KOiK04153.
OMAiIALPWKV.
OrthoDBiEOG7NCV3Q.
PhylomeDBiP28564.
TreeFamiTF316350.

Enzyme and pathway databases

ReactomeiREACT_339234. G alpha (i) signalling events.
REACT_343696. Serotonin receptors.

Miscellaneous databases

NextBioi605318.
PROiP28564.

Gene expression databases

GenevestigatoriP28564.

Family and domain databases

InterProiIPR002147. 5HT1B_rcpt.
IPR002231. 5HT_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24247:SF16. PTHR24247:SF16. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00513. 5HT1BRECEPTR.
PR01101. 5HTRECEPTOR.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Distinct 5-HT1B and 5-HT1D serotonin receptors in rat: structural and pharmacological comparison of the two cloned receptors."
    Hamblin M.W., McGuffin R.W., Metcalf M.A., Dorsa D.M., Merchant K.M.
    Mol. Cell. Neurosci. 3:578-587(1992)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
  2. "Molecular cloning and characterization of a rat brain cDNA encoding a 5-hydroxytryptamine1B receptor."
    Voigt M.M., Laurie D.J., Seeburg P.H., Bach A.
    EMBO J. 10:4017-4023(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
    Tissue: Brain.

Entry informationi

Entry namei5HT1B_RAT
AccessioniPrimary (citable) accession number: P28564
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: April 1, 2015
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

A residue in the 7th transmembrane region ('Thr-355' in human, Asn-351 in mouse and rat) is important for species-specific sensitivity to various agonists.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.