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P28564

- 5HT1B_RAT

UniProt

P28564 - 5HT1B_RAT

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Protein
5-hydroxytryptamine receptor 1B
Gene
Htr1b, 5ht1b
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various alkaloids and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity. Arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Regulates the release of 5-hydroxytryptamine, dopamine and acetylcholine in the brain, and thereby affects neural activity, nociceptive processing, pain perception, mood and behavior. Besides, plays a role in vasoconstriction of cerebral arteries.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei351 – 3511Important for species-specific agonist sensitivity By similarity

GO - Molecular functioni

  1. drug binding Source: RGD
  2. serotonin binding Source: RGD
  3. serotonin receptor activity Source: RGD

GO - Biological processi

  1. G-protein coupled receptor internalization Source: Ensembl
  2. adenylate cyclase-inhibiting serotonin receptor signaling pathway Source: UniProtKB
  3. bone remodeling Source: Ensembl
  4. cellular response to alkaloid Source: UniProtKB
  5. cellular response to drug Source: UniProtKB
  6. cellular response to temperature stimulus Source: Ensembl
  7. drinking behavior Source: RGD
  8. feeding behavior Source: RGD
  9. negative regulation of cAMP biosynthetic process Source: Ensembl
  10. negative regulation of serotonin secretion Source: UniProtKB
  11. negative regulation of synaptic transmission, GABAergic Source: RGD
  12. negative regulation of synaptic transmission, glutamatergic Source: RGD
  13. protein kinase C-activating G-protein coupled receptor signaling pathway Source: Ensembl
  14. regulation of behavior Source: InterPro
  15. regulation of dopamine secretion Source: RGD
  16. response to cocaine Source: RGD
  17. response to ethanol Source: RGD
  18. response to mineralocorticoid Source: RGD
  19. synaptic transmission Source: RGD
  20. vasoconstriction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Behavior

Names & Taxonomyi

Protein namesi
Recommended name:
5-hydroxytryptamine receptor 1B
Short name:
5-HT-1B
Short name:
5-HT1B
Alternative name(s):
Serotonin receptor 1B
Gene namesi
Name:Htr1b
Synonyms:5ht1b
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 8

Organism-specific databases

RGDi2846. Htr1b.

Subcellular locationi

Cell membrane; Multi-pass membrane protein 2 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4545Extracellular By similarity
Add
BLAST
Transmembranei46 – 7126Helical; Name=1; By similarity
Add
BLAST
Topological domaini72 – 809Cytoplasmic By similarity
Transmembranei81 – 10626Helical; Name=2; By similarity
Add
BLAST
Topological domaini107 – 11913Extracellular By similarity
Add
BLAST
Transmembranei120 – 14122Helical; Name=3; By similarity
Add
BLAST
Topological domaini142 – 16120Cytoplasmic By similarity
Add
BLAST
Transmembranei162 – 18322Helical; Name=4; By similarity
Add
BLAST
Topological domaini184 – 20118Extracellular By similarity
Add
BLAST
Transmembranei202 – 22423Helical; Name=5; By similarity
Add
BLAST
Topological domaini225 – 31187Cytoplasmic By similarity
Add
BLAST
Transmembranei312 – 33221Helical; Name=6; By similarity
Add
BLAST
Topological domaini333 – 34513Extracellular By similarity
Add
BLAST
Transmembranei346 – 36722Helical; Name=7; By similarity
Add
BLAST
Topological domaini368 – 38619Cytoplasmic By similarity
Add
BLAST

GO - Cellular componenti

  1. cytoplasm Source: Ensembl
  2. integral component of plasma membrane Source: UniProtKB
  3. plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3863865-hydroxytryptamine receptor 1B
PRO_0000068921Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi24 – 241N-linked (GlcNAc...) Reviewed prediction
Glycosylationi28 – 281N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi118 ↔ 195 By similarity
Lipidationi384 – 3841S-palmitoyl cysteine Reviewed prediction

Post-translational modificationi

Phosphorylated By similarity.
Palmitoylated By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PRIDEiP28564.

PTM databases

PhosphoSiteiP28564.

Expressioni

Gene expression databases

GenevestigatoriP28564.

Interactioni

Subunit structurei

Homodimer. Heterodimer with HTR1D By similarity.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017411.

Structurei

3D structure databases

ProteinModelPortaliP28564.
SMRiP28564. Positions 130-161.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni121 – 13010Agonist binding By similarity
Regioni323 – 3275Agonist binding By similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi142 – 1443DRY motif; important for ligand-induced conformation changes and signaling By similarity
Motifi361 – 3655NPxxY motif; important for ligand-induced conformation changes and signaling By similarity

Domaini

Ligands are bound in a hydrophobic pocket formed by the transmembrane helices By similarity.

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG249628.
GeneTreeiENSGT00750000117301.
HOGENOMiHOG000239242.
HOVERGENiHBG106962.
InParanoidiP28564.
KOiK04153.
OMAiIALPWKV.
OrthoDBiEOG7NCV3Q.
PhylomeDBiP28564.
TreeFamiTF316350.

Family and domain databases

Gene3Di1.20.1070.10. 2 hits.
InterProiIPR002147. 5HT1B_rcpt.
IPR002231. 5HT_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24247:SF16. PTHR24247:SF16. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00513. 5HT1BRECEPTR.
PR01101. 5HTRECEPTOR.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28564-1 [UniParc]FASTAAdd to Basket

« Hide

MEEQGIQCAP PPPATSQTGV PLANLSHNCS ADDYIYQDSI ALPWKVLLVA    50
LLALITLATT LSNAFVIATV YRTRKLHTPA NYLIASLAVT DLLVSILVMP 100
ISTMYTVTGR WTLGQVVCDF WLSSDITCCT ASIMHLCVIA LDRYWAITDA 150
VDYSAKRTPK RAAIMIVLVW VFSISISLPP FFWRQAKAEE EVLDCFVNTD 200
HVLYTVYSTV GAFYLPTLLL IALYGRIYVE ARSRILKQTP NKTGKRLTRA 250
QLITDSPGST SSVTSINSRV PEVPSESGSP VYVNQVKVRV SDALLEKKKL 300
MAARERKATK TLGIILGAFI VCWLPFFIIS LVMPICKDAC WFHMAIFDFF 350
NWLGYLNSLI NPIIYTMSNE DFKQAFHKLI RFKCTG 386
Length:386
Mass (Da):43,163
Last modified:December 1, 1992 - v1
Checksum:i4F4E9AC1C9AED214
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M89954 Genomic DNA. Translation: AAA40613.1.
X62944 mRNA. Translation: CAA44716.1.
PIRiS18637.
RefSeqiNP_071561.1. NM_022225.1.
UniGeneiRn.138109.

Genome annotation databases

EnsembliENSRNOT00000017411; ENSRNOP00000017411; ENSRNOG00000013042.
GeneIDi25075.
KEGGirno:25075.
UCSCiRGD:2846. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M89954 Genomic DNA. Translation: AAA40613.1 .
X62944 mRNA. Translation: CAA44716.1 .
PIRi S18637.
RefSeqi NP_071561.1. NM_022225.1.
UniGenei Rn.138109.

3D structure databases

ProteinModelPortali P28564.
SMRi P28564. Positions 130-161.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000017411.

Chemistry

BindingDBi P28564.
ChEMBLi CHEMBL2095159.
GuidetoPHARMACOLOGYi 2.

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei P28564.

Proteomic databases

PRIDEi P28564.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000017411 ; ENSRNOP00000017411 ; ENSRNOG00000013042 .
GeneIDi 25075.
KEGGi rno:25075.
UCSCi RGD:2846. rat.

Organism-specific databases

CTDi 3351.
RGDi 2846. Htr1b.

Phylogenomic databases

eggNOGi NOG249628.
GeneTreei ENSGT00750000117301.
HOGENOMi HOG000239242.
HOVERGENi HBG106962.
InParanoidi P28564.
KOi K04153.
OMAi IALPWKV.
OrthoDBi EOG7NCV3Q.
PhylomeDBi P28564.
TreeFami TF316350.

Miscellaneous databases

NextBioi 605318.
PROi P28564.

Gene expression databases

Genevestigatori P28564.

Family and domain databases

Gene3Di 1.20.1070.10. 2 hits.
InterProi IPR002147. 5HT1B_rcpt.
IPR002231. 5HT_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view ]
PANTHERi PTHR24247:SF16. PTHR24247:SF16. 1 hit.
Pfami PF00001. 7tm_1. 1 hit.
[Graphical view ]
PRINTSi PR00513. 5HT1BRECEPTR.
PR01101. 5HTRECEPTOR.
PR00237. GPCRRHODOPSN.
PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Distinct 5-HT1B and 5-HT1D serotonin receptors in rat: structural and pharmacological comparison of the two cloned receptors."
    Hamblin M.W., McGuffin R.W., Metcalf M.A., Dorsa D.M., Merchant K.M.
    Mol. Cell. Neurosci. 3:578-587(1992)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
  2. "Molecular cloning and characterization of a rat brain cDNA encoding a 5-hydroxytryptamine1B receptor."
    Voigt M.M., Laurie D.J., Seeburg P.H., Bach A.
    EMBO J. 10:4017-4023(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
    Tissue: Brain.

Entry informationi

Entry namei5HT1B_RAT
AccessioniPrimary (citable) accession number: P28564
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: April 16, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

A residue in the 7th transmembrane region ('Thr-355' in human, Asn-351 in mouse and rat) is important for species-specific sensitivity to various agonists By similarity.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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