Reviewed, UniProtKB/Swiss-Prot P28563 (DUS1_MOUSE)

Names and origin

Protein names

Recommended name:
    Dual specificity protein phosphatase 1
    EC=3.1.3.48
    EC=3.1.3.16
Alternative name(s):
    MAP kinase phosphatase 1
      Short name=MKP-1
    Protein-tyrosine phosphatase 3CH134
    Protein-tyrosine phosphatase ERP

Gene names

Name:	Dusp1
Synonyms:	3ch134, Mkp1, Ptpn10, Ptpn16

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Dual specificity phosphatase that dephosphorylates MAP kinase ERK2 on both 'Thr-183' and 'Tyr-185'.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. A phosphoprotein + H2O = a protein + phosphate.
Induction	By growth factors.
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily. Contains 1 rhodanese domain. Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
Biological process	Cell cycle
Molecular function	Hydrolase Protein phosphatase
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid dephosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	MAP kinase tyrosine/serine/threonine phosphatase activity Inferred from electronic annotation. Source: InterPro protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 367	367	Dual specificity protein phosphatase 1		PRO_0000094791
Regions
	Domain	20 – 137	118	Rhodanese
	Domain	175 – 367	193	Tyrosine-protein phosphatase
Sites
	Active site	258	1	Phosphocysteine intermediate
Experimental info
	Mutagenesis	258	1	C → S: Loss of activity.

Sequences

	Sequence		Length	Mass (Da)
	P28563-1 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 50B5F90FEBBD19AB	FASTA	367	39,370
	10 20 30 40 50 60 MVMEVGILDA GGLRALLREG AAQCLLLDCR SFFAFNAGHI AGSVNVRFST IVRRRAKGAM 70 80 90 100 110 120 GLEHIVPNAE LRGRLLAGAY HAVVLLDERS ASLDGAKRDG TLALAAGALC REARSTQVFF 130 140 150 160 170 180 LQGGYEAFSA SCPELCSKQS TPTGLSLPLS TSVPDSAESG CSSCSTPLYD QGGPVEILSF 190 200 210 220 230 240 LYLGSAYHAS RKDMLDALGI TALINVSANC PNHFEGHYQY KSIPVEDNHK ADISSWFNEA 250 260 270 280 290 300 IDFIDSIKDA GGRVFVHCQA GISRSATICL AYLMRTNRVK LDEAFEFVKQ RRSIISPNFS 310 320 330 340 350 360 FMGQLLQFES QVLAPHCSAE AGSPAMAVLD RGTSTTTVFN FPVSIPVHPT NSALNYLKSP ITTSPSC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA sequence of a growth factor-inducible immediate early gene and characterization of its encoded protein." Charles C.H., Abler A.S., Lau L.F. Oncogene 7:187-190(1992) [PubMed: 1741163] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c.
[2]	"Structure, mapping, and expression of erp, a growth factor-inducible gene encoding a nontransmembrane protein tyrosine phosphatase, and effect of ERP on cell growth." Noguchi T., Metz R., Chen L., Mattei M.-G., Carrasco D., Bravo R. Mol. Cell. Biol. 13:5195-5205(1993) [PubMed: 8355678] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Tissue: Liver.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Czech II. Tissue: Mammary gland.
[4]	"MKP-1 (3CH134), an immediate early gene product, is a dual specificity phosphatase that dephosphorylates MAP kinase in vivo." Sun H., Charles C.H., Lau L.F., Tonks N.K. Cell 75:487-493(1993) [PubMed: 8221888] [Abstract] Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X61940 mRNA. Translation: CAA43944.1. S64851 Genomic DNA. Translation: AAB27882.1. BC006967 mRNA. Translation: AAH06967.1.
IPI	IPI00122070.
PIR	S24411. A54681.
RefSeq	NP_038670.1.
UniGene	Mm.239041
3D structure databases
HSSP	HSSP built from PDB template 1M3G based on UniProtKB Q05923.
SMR	P28563. Positions 171-313.
ModBase	Search...
PTM databases
PhosphoSite	P28563.
Genome annotation databases
Ensembl	ENSMUSG00000024190. Mus musculus. [Contig view]
GeneID	19252.
KEGG	mmu:19252.
Organism-specific databases
MGI	MGI:105120. Dusp1.
Phylogenomic databases
HOGENOM	P28563.
HOVERGEN	P28563.
OMA	P28563. MEVGILD.
Enzyme and pathway databases
BRENDA	3.1.3.16. 244. 3.1.3.48. 244.
Gene expression databases
ArrayExpress	P28563.
Bgee	P28563.
CleanEx	MM_DUSP1.
GermOnline	ENSMUSG00000024190. Mus musculus.
Family and domain databases
InterPro	IPR014393. Dual_MAP_Kinase_Phosphatase. IPR008343. MAPK_phosph. IPR001763. Rhodanese-like. IPR000387. Tyr_Pase. IPR016130. Tyr_Pase_AS. IPR000340. Tyr_Pase_dual_specific. [Graphical view]
Gene3D	G3DSA:3.40.250.10. Rhodanese-like. 1 hit.
Pfam	PF00782. DSPc. 1 hit. PF00581. Rhodanese. 1 hit. [Graphical view]
PIRSF	PIRSF000939. MAPK_Ptase. 1 hit.
PRINTS	PR01764. MAPKPHPHTASE.
SMART	SM00195. DSPc. 1 hit. SM00450. RHOD. 1 hit. [Graphical view]
PROSITE	PS50206. RHODANESE_3. 1 hit. PS00383. TYR_PHOSPHATASE_1. 1 hit. PS50056. TYR_PHOSPHATASE_2. 1 hit. PS50054. TYR_PHOSPHATASE_DUAL. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	296092.
SOURCE	Search...

Entry information

Entry name

DUS1_MOUSE

Accession

Primary (citable) accession number: P28563

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	June 16, 2009
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)