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P28562

- DUS1_HUMAN

UniProt

P28562 - DUS1_HUMAN

Protein

Dual specificity protein phosphatase 1

Gene

DUSP1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 145 (01 Oct 2014)
      Sequence version 3 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Dual specificity phosphatase that dephosphorylates MAP kinase MAPK1/ERK2 on both 'Thr-183' and 'Tyr-185', regulating its activity during the meiotic cell cycle.By similarity

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation
    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei258 – 2581Phosphocysteine intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. MAP kinase tyrosine/serine/threonine phosphatase activity Source: UniProtKB
    2. non-membrane spanning protein tyrosine phosphatase activity Source: ProtInc
    3. protein binding Source: IntAct
    4. protein tyrosine/serine/threonine phosphatase activity Source: RefGenome
    5. protein tyrosine/threonine phosphatase activity Source: Ensembl

    GO - Biological processi

    1. cellular response to hormone stimulus Source: Ensembl
    2. endoderm formation Source: RefGenome
    3. inactivation of MAPK activity Source: UniProtKB
    4. intracellular signal transduction Source: Ensembl
    5. mitotic cell cycle arrest Source: UniProtKB
    6. negative regulation of apoptotic process Source: Ensembl
    7. negative regulation of MAPK cascade Source: UniProtKB
    8. negative regulation of MAP kinase activity Source: UniProtKB
    9. negative regulation of meiotic cell cycle Source: UniProtKB
    10. peptidyl-threonine dephosphorylation Source: UniProtKB
    11. peptidyl-tyrosine dephosphorylation Source: UniProtKB
    12. positive regulation of apoptotic process Source: Ensembl
    13. protein dephosphorylation Source: RefGenome
    14. regulation of apoptotic process Source: RefGenome
    15. regulation of mitotic cell cycle spindle assembly checkpoint Source: UniProtKB
    16. response to calcium ion Source: Ensembl
    17. response to cAMP Source: Ensembl
    18. response to estradiol Source: Ensembl
    19. response to glucocorticoid Source: Ensembl
    20. response to hydrogen peroxide Source: Ensembl
    21. response to light stimulus Source: Ensembl
    22. response to oxidative stress Source: ProtInc
    23. response to retinoic acid Source: Ensembl
    24. response to testosterone Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Biological processi

    Cell cycle, Stress response

    Enzyme and pathway databases

    SABIO-RKP28562.
    SignaLinkiP28562.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dual specificity protein phosphatase 1 (EC:3.1.3.16, EC:3.1.3.48)
    Alternative name(s):
    Dual specificity protein phosphatase hVH1
    Mitogen-activated protein kinase phosphatase 1
    Short name:
    MAP kinase phosphatase 1
    Short name:
    MKP-1
    Protein-tyrosine phosphatase CL100
    Gene namesi
    Name:DUSP1
    Synonyms:CL100, MKP1, PTPN10, VH1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 5

    Organism-specific databases

    HGNCiHGNC:3064. DUSP1.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA27519.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 367367Dual specificity protein phosphatase 1PRO_0000094790Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei359 – 3591Phosphoserine; by MAPK1 and MAPK3By similarity
    Modified residuei364 – 3641Phosphoserine; by MAPK1 and MAPK3By similarity

    Post-translational modificationi

    Phosphorylation at Ser-359 and Ser-364 by MAPK1/ERK2 and MAPK3/ERK1 reduces its rate of degradation.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP28562.
    PaxDbiP28562.
    PRIDEiP28562.

    PTM databases

    PhosphoSiteiP28562.

    Expressioni

    Tissue specificityi

    Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney.1 Publication

    Inductioni

    By oxidative stress and heat shock.

    Gene expression databases

    ArrayExpressiP28562.
    BgeeiP28562.
    CleanExiHS_DUSP1.
    GenevestigatoriP28562.

    Organism-specific databases

    HPAiCAB018554.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MAPK1P284826EBI-975493,EBI-959949
    MAPK14Q165394EBI-975493,EBI-73946
    MAPK3P273615EBI-975493,EBI-73995
    SKP2Q133093EBI-975493,EBI-456291
    UBBP0CG472EBI-975493,EBI-413034

    Protein-protein interaction databases

    BioGridi108176. 16 interactions.
    IntActiP28562. 15 interactions.
    MINTiMINT-8217028.
    STRINGi9606.ENSP00000239223.

    Structurei

    3D structure databases

    ProteinModelPortaliP28562.
    SMRiP28562. Positions 13-136, 172-314.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini20 – 137118RhodanesePROSITE-ProRule annotationAdd
    BLAST
    Domaini175 – 367193Tyrosine-protein phosphataseAdd
    BLAST

    Sequence similaritiesi

    Contains 1 rhodanese domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG2453.
    HOGENOMiHOG000294080.
    HOVERGENiHBG007347.
    InParanoidiP28562.
    KOiK04459.
    OMAiSFNSSHI.
    OrthoDBiEOG75MVWD.
    PhylomeDBiP28562.
    TreeFamiTF105122.

    Family and domain databases

    Gene3Di3.40.250.10. 1 hit.
    3.90.190.10. 1 hit.
    InterProiIPR020417. Atypical_DUSP.
    IPR000340. Dual-sp_phosphatase_cat-dom.
    IPR020422. Dual-sp_phosphatase_subgr_cat.
    IPR024950. DUSP.
    IPR008343. MKP.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR001763. Rhodanese-like_dom.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    [Graphical view]
    PANTHERiPTHR10159. PTHR10159. 1 hit.
    PfamiPF00782. DSPc. 1 hit.
    PF00581. Rhodanese. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000939. MAPK_Ptase. 1 hit.
    PRINTSiPR01908. ADSPHPHTASE.
    PR01764. MAPKPHPHTASE.
    SMARTiSM00195. DSPc. 1 hit.
    SM00450. RHOD. 1 hit.
    [Graphical view]
    SUPFAMiSSF52799. SSF52799. 1 hit.
    SSF52821. SSF52821. 1 hit.
    PROSITEiPS50206. RHODANESE_3. 1 hit.
    PS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P28562-1 [UniParc]FASTAAdd to Basket

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    MVMEVGTLDA GGLRALLGER AAQCLLLDCR SFFAFNAGHI AGSVNVRFST    50
    IVRRRAKGAM GLEHIVPNAE LRGRLLAGAY HAVVLLDERS AALDGAKRDG 100
    TLALAAGALC REARAAQVFF LKGGYEAFSA SCPELCSKQS TPMGLSLPLS 150
    TSVPDSAESG CSSCSTPLYD QGGPVEILPF LYLGSAYHAS RKDMLDALGI 200
    TALINVSANC PNHFEGHYQY KSIPVEDNHK ADISSWFNEA IDFIDSIKNA 250
    GGRVFVHCQA GISRSATICL AYLMRTNRVK LDEAFEFVKQ RRSIISPNFS 300
    FMGQLLQFES QVLAPHCSAE AGSPAMAVLD RGTSTTTVFN FPVSIPVHST 350
    NSALSYLQSP ITTSPSC 367
    Length:367
    Mass (Da):39,298
    Last modified:February 1, 1996 - v3
    Checksum:i11BD1D39A9FCD51F
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti56 – 561A → T.1 Publication
    Corresponds to variant rs34013988 [ dbSNP | Ensembl ].
    VAR_025201
    Natural varianti187 – 1871Y → H.1 Publication
    Corresponds to variant rs34471628 [ dbSNP | Ensembl ].
    VAR_025202

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X68277 mRNA. Translation: CAA48338.1.
    DQ301957 Genomic DNA. Translation: ABB96250.1.
    CH471062 Genomic DNA. Translation: EAW61425.1.
    CH471062 Genomic DNA. Translation: EAW61426.1.
    BC022463 mRNA. Translation: AAH22463.1.
    CCDSiCCDS4380.1.
    PIRiS29090.
    RefSeqiNP_004408.1. NM_004417.3.
    UniGeneiHs.171695.

    Genome annotation databases

    EnsembliENST00000239223; ENSP00000239223; ENSG00000120129.
    GeneIDi1843.
    KEGGihsa:1843.
    UCSCiuc003mbv.2. human.

    Polymorphism databases

    DMDMi1346900.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Web resourcesi

    NIEHS-SNPs
    Atlas of Genetics and Cytogenetics in Oncology and Haematology

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X68277 mRNA. Translation: CAA48338.1 .
    DQ301957 Genomic DNA. Translation: ABB96250.1 .
    CH471062 Genomic DNA. Translation: EAW61425.1 .
    CH471062 Genomic DNA. Translation: EAW61426.1 .
    BC022463 mRNA. Translation: AAH22463.1 .
    CCDSi CCDS4380.1.
    PIRi S29090.
    RefSeqi NP_004408.1. NM_004417.3.
    UniGenei Hs.171695.

    3D structure databases

    ProteinModelPortali P28562.
    SMRi P28562. Positions 13-136, 172-314.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 108176. 16 interactions.
    IntActi P28562. 15 interactions.
    MINTi MINT-8217028.
    STRINGi 9606.ENSP00000239223.

    Chemistry

    BindingDBi P28562.
    ChEMBLi CHEMBL6026.

    PTM databases

    PhosphoSitei P28562.

    Polymorphism databases

    DMDMi 1346900.

    Proteomic databases

    MaxQBi P28562.
    PaxDbi P28562.
    PRIDEi P28562.

    Protocols and materials databases

    DNASUi 1843.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000239223 ; ENSP00000239223 ; ENSG00000120129 .
    GeneIDi 1843.
    KEGGi hsa:1843.
    UCSCi uc003mbv.2. human.

    Organism-specific databases

    CTDi 1843.
    GeneCardsi GC05M172195.
    HGNCi HGNC:3064. DUSP1.
    HPAi CAB018554.
    MIMi 600714. gene.
    neXtProti NX_P28562.
    PharmGKBi PA27519.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG2453.
    HOGENOMi HOG000294080.
    HOVERGENi HBG007347.
    InParanoidi P28562.
    KOi K04459.
    OMAi SFNSSHI.
    OrthoDBi EOG75MVWD.
    PhylomeDBi P28562.
    TreeFami TF105122.

    Enzyme and pathway databases

    SABIO-RK P28562.
    SignaLinki P28562.

    Miscellaneous databases

    ChiTaRSi DUSP1. human.
    GeneWikii DUSP1.
    GenomeRNAii 1843.
    NextBioi 7547.
    PROi P28562.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P28562.
    Bgeei P28562.
    CleanExi HS_DUSP1.
    Genevestigatori P28562.

    Family and domain databases

    Gene3Di 3.40.250.10. 1 hit.
    3.90.190.10. 1 hit.
    InterProi IPR020417. Atypical_DUSP.
    IPR000340. Dual-sp_phosphatase_cat-dom.
    IPR020422. Dual-sp_phosphatase_subgr_cat.
    IPR024950. DUSP.
    IPR008343. MKP.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR001763. Rhodanese-like_dom.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    [Graphical view ]
    PANTHERi PTHR10159. PTHR10159. 1 hit.
    Pfami PF00782. DSPc. 1 hit.
    PF00581. Rhodanese. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000939. MAPK_Ptase. 1 hit.
    PRINTSi PR01908. ADSPHPHTASE.
    PR01764. MAPKPHPHTASE.
    SMARTi SM00195. DSPc. 1 hit.
    SM00450. RHOD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52799. SSF52799. 1 hit.
    SSF52821. SSF52821. 1 hit.
    PROSITEi PS50206. RHODANESE_3. 1 hit.
    PS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Oxidative stress and heat shock induce a human gene encoding a protein-tyrosine phosphatase."
      Keyes S.M., Emslie E.A.
      Nature 359:644-647(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Foreskin.
    2. "Isolation and characterization of a human dual specificity protein-tyrosine phosphatase gene."
      Kwak S.P., Hakes D.J., Martell K.J., Dixon J.E.
      J. Biol. Chem. 269:3596-3604(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    3. NIEHS SNPs program
      Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS THR-56 AND HIS-187.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiDUS1_HUMAN
    AccessioniPrimary (citable) accession number: P28562
    Secondary accession number(s): D3DQL9, Q2V508
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 145 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 5
      Human chromosome 5: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3