Reviewed, UniProtKB/Swiss-Prot P28562 (DUS1_HUMAN)

Names and origin

Protein names

Recommended name:
    Dual specificity protein phosphatase 1
    EC=3.1.3.48
    EC=3.1.3.16
Alternative name(s):
    MAP kinase phosphatase 1
      Short name=MKP-1
    Protein-tyrosine phosphatase CL100
    Dual specificity protein phosphatase hVH1

Gene names

Name:	DUSP1
Synonyms:	CL100, MKP1, PTPN10, VH1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Dual specificity phosphatase that dephosphorylates MAP kinase ERK2 on both 'Thr-183' and 'Tyr-185'.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. A phosphoprotein + H2O = a protein + phosphate.
Tissue specificity	Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney. Ref.2
Induction	By oxidative stress and heat shock.
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily. Contains 1 rhodanese domain. Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
Biological process	Cell cycle Stress response
Coding sequence diversity	Polymorphism
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid dephosphorylation Inferred from electronic annotation. Source: InterPro response to oxidative stress Ref.1 Traceable author statement. Source: ProtInc
Molecular function	MAP kinase tyrosine/serine/threonine phosphatase activity Inferred from electronic annotation. Source: InterPro non-membrane spanning protein tyrosine phosphatase activity Ref.1 Traceable author statement. Source: ProtInc protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MAPK1	P28482	2	EBI-975493,EBI-959949
RPS27A	P62988	1	EBI-975493,EBI-413034
SKP2	Q13309	1	EBI-975493,EBI-456291

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 367	367	Dual specificity protein phosphatase 1		PRO_0000094790
Regions
	Domain	20 – 137	118	Rhodanese
	Domain	175 – 367	193	Tyrosine-protein phosphatase
Sites
	Active site	258	1	Phosphocysteine intermediate By similarity
Natural variations
	Natural variant	56	1	A → T Ref.3		VAR_025201
	Natural variant	187	1	Y → H: dbSNP rs34471628. Ref.3		VAR_025202

Sequences

	Sequence		Length	Mass (Da)
	P28562-1 [UniParc]. Last modified February 1, 1996. Version 3. Checksum: 11BD1D39A9FCD51F	FASTA	367	39,298
	10 20 30 40 50 60 MVMEVGTLDA GGLRALLGER AAQCLLLDCR SFFAFNAGHI AGSVNVRFST IVRRRAKGAM 70 80 90 100 110 120 GLEHIVPNAE LRGRLLAGAY HAVVLLDERS AALDGAKRDG TLALAAGALC REARAAQVFF 130 140 150 160 170 180 LKGGYEAFSA SCPELCSKQS TPMGLSLPLS TSVPDSAESG CSSCSTPLYD QGGPVEILPF 190 200 210 220 230 240 LYLGSAYHAS RKDMLDALGI TALINVSANC PNHFEGHYQY KSIPVEDNHK ADISSWFNEA 250 260 270 280 290 300 IDFIDSIKNA GGRVFVHCQA GISRSATICL AYLMRTNRVK LDEAFEFVKQ RRSIISPNFS 310 320 330 340 350 360 FMGQLLQFES QVLAPHCSAE AGSPAMAVLD RGTSTTTVFN FPVSIPVHST NSALSYLQSP ITTSPSC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Oxidative stress and heat shock induce a human gene encoding a protein-tyrosine phosphatase." Keyes S.M., Emslie E.A. Nature 359:644-647(1992) [PubMed: 1406996] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Foreskin.
[2]	"Isolation and characterization of a human dual specificity protein-tyrosine phosphatase gene." Kwak S.P., Hakes D.J., Martell K.J., Dixon J.E. J. Biol. Chem. 269:3596-3604(1994) [PubMed: 8106404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[3]	NIEHS SNPs program Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS THR-56 AND HIS-187.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
+	Additional computationally mapped references.

Web resources

NIEHS-SNPs

Cross-references

Sequence databases
EMBLGenBankDDBJ	X68277 mRNA. Translation: CAA48338.1. DQ301957 Genomic DNA. Translation: ABB96250.1. BC022463 mRNA. Translation: AAH22463.1.
IPI	IPI00003928.
PIR	S29090.
RefSeq	NP_004408.1.
UniGene	Hs.171695 Hs.710943
3D structure databases
HSSP	HSSP built from PDB template 1M3G based on UniProtKB Q05923.
SMR	P28562. Positions 171-313.
ModBase	Search...
Protein-protein interaction databases
IntAct	P28562. 7 interactions.
PTM databases
PhosphoSite	P28562.
Proteomic databases
PRIDE	P28562.
Genome annotation databases
Ensembl	ENSG00000120129. Homo sapiens. [Contig view]
GeneID	1843.
KEGG	hsa:1843.
UCSC	uc003mbv.1. human.
Organism-specific databases
GeneCards	GC05M172127.
H-InvDB	HIX0005420.
HGNC	HGNC:3064. DUSP1.
HPA	CAB018554.
MIM	600714. gene.
PharmGKB	PA27519.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	P28562.
HOVERGEN	P28562.
OMA	P28562. MEVGILD.
Enzyme and pathway databases
BRENDA	3.1.3.16. 247. 3.1.3.48. 247.
Pathway_Interaction_DB	fcer1pathway. Fc-epsilon receptor I signaling in mast cells. p38alphabetapathway. Regulation of p38-alpha and p38-beta.
Gene expression databases
ArrayExpress	P28562.
Bgee	P28562.
CleanEx	HS_DUSP1.
GermOnline	ENSG00000120129. Homo sapiens.
Family and domain databases
InterPro	IPR014393. Dual_MAP_Kinase_Phosphatase. IPR008343. MAPK_phosph. IPR001763. Rhodanese-like. IPR000387. Tyr_Pase. IPR016130. Tyr_Pase_AS. IPR000340. Tyr_Pase_dual_specific. [Graphical view]
Gene3D	G3DSA:3.40.250.10. Rhodanese-like. 1 hit.
Pfam	PF00782. DSPc. 1 hit. PF00581. Rhodanese. 1 hit. [Graphical view]
PIRSF	PIRSF000939. MAPK_Ptase. 1 hit.
PRINTS	PR01764. MAPKPHPHTASE.
SMART	SM00195. DSPc. 1 hit. SM00450. RHOD. 1 hit. [Graphical view]
PROSITE	PS50206. RHODANESE_3. 1 hit. PS00383. TYR_PHOSPHATASE_1. 1 hit. PS50056. TYR_PHOSPHATASE_2. 1 hit. PS50054. TYR_PHOSPHATASE_DUAL. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	7547.
SOURCE	Search...

Entry information

Entry name

DUS1_HUMAN

Accession

Primary (citable) accession number: P28562
Secondary accession number(s): Q2V508

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	February 1, 1996
Last modified:	July 7, 2009
This is version 93 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)