P28562 (DUS1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dual specificity protein phosphatase 1
EC=3.1.3.16
EC=3.1.3.48

Alternative name(s):
Dual specificity protein phosphatase hVH1
Mitogen-activated protein kinase phosphatase 1
Short name=MAP kinase phosphatase 1
Short name=MKP-1
Protein-tyrosine phosphatase CL100

Gene names

Name:	DUSP1
Synonyms:	CL100, MKP1, PTPN10, VH1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Dual specificity phosphatase that dephosphorylates MAP kinase MAPK1/ERK2 on both 'Thr-183' and 'Tyr-185', regulating its activity during the meiotic cell cycle By similarity.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. A phosphoprotein + H2O = a protein + phosphate.
Subcellular location	Nucleus By similarity.
Tissue specificity	Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney. Ref.2
Induction	By oxidative stress and heat shock.
Post-translational modification	Phosphorylation at Ser-359 and Ser-364 by MAPK1/ERK2 and MAPK3/ERK1 reduces its rate of degradation By similarity.
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily. Contains 1 rhodanese domain. Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
Biological process	Cell cycle Stress response
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Molecular function	Hydrolase Protein phosphatase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cellular response to hormone stimulus Inferred from electronic annotation. Source: Compara endoderm formation Inferred from Biological aspect of Ancestor. Source: RefGenome intracellular signal transduction Inferred from electronic annotation. Source: Compara positive regulation of apoptotic process Inferred from electronic annotation. Source: Compara regulation of apoptotic process Inferred from Biological aspect of Ancestor. Source: RefGenome response to cAMP Inferred from electronic annotation. Source: Compara response to calcium ion Inferred from electronic annotation. Source: Compara response to estradiol stimulus Inferred from electronic annotation. Source: Compara response to glucocorticoid stimulus Inferred from electronic annotation. Source: Compara response to hydrogen peroxide Inferred from electronic annotation. Source: Compara response to light stimulus Inferred from electronic annotation. Source: Compara response to oxidative stress Traceable author statement Ref.1. Source: ProtInc response to retinoic acid Inferred from electronic annotation. Source: Compara response to testosterone stimulus Inferred from electronic annotation. Source: Compara
Cellular_component	nucleoplasm Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	MAP kinase tyrosine/serine/threonine phosphatase activity Inferred from Biological aspect of Ancestor. Source: RefGenome non-membrane spanning protein tyrosine phosphatase activity Traceable author statement Ref.1. Source: ProtInc protein tyrosine/threonine phosphatase activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MAPK1	P28482	3	EBI-975493,EBI-959949
SKP2	Q13309	3	EBI-975493,EBI-456291
UBB	P0CG47	2	EBI-975493,EBI-413034

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 367	367	Dual specificity protein phosphatase 1		PRO_0000094790
Regions
	Domain	20 – 137	118	Rhodanese
	Domain	175 – 367	193	Tyrosine-protein phosphatase
Sites
	Active site	258	1	Phosphocysteine intermediate By similarity
Amino acid modifications
	Modified residue	359	1	Phosphoserine; by MAPK1 and MAPK3 By similarity
	Modified residue	364	1	Phosphoserine; by MAPK1 and MAPK3 By similarity
Natural variations
	Natural variant	56	1	A → T. Ref.3 Corresponds to variant rs34013988 [ dbSNP | Ensembl ].		VAR_025201
	Natural variant	187	1	Y → H. Ref.3 Corresponds to variant rs34471628 [ dbSNP | Ensembl ].		VAR_025202

Sequences

	Sequence		Length	Mass (Da)
	P28562 [UniParc]. Last modified February 1, 1996. Version 3. Checksum: 11BD1D39A9FCD51F	FASTA	367	39,298
	10 20 30 40 50 60 MVMEVGTLDA GGLRALLGER AAQCLLLDCR SFFAFNAGHI AGSVNVRFST IVRRRAKGAM 70 80 90 100 110 120 GLEHIVPNAE LRGRLLAGAY HAVVLLDERS AALDGAKRDG TLALAAGALC REARAAQVFF 130 140 150 160 170 180 LKGGYEAFSA SCPELCSKQS TPMGLSLPLS TSVPDSAESG CSSCSTPLYD QGGPVEILPF 190 200 210 220 230 240 LYLGSAYHAS RKDMLDALGI TALINVSANC PNHFEGHYQY KSIPVEDNHK ADISSWFNEA 250 260 270 280 290 300 IDFIDSIKNA GGRVFVHCQA GISRSATICL AYLMRTNRVK LDEAFEFVKQ RRSIISPNFS 310 320 330 340 350 360 FMGQLLQFES QVLAPHCSAE AGSPAMAVLD RGTSTTTVFN FPVSIPVHST NSALSYLQSP ITTSPSC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Oxidative stress and heat shock induce a human gene encoding a protein-tyrosine phosphatase." Keyes S.M., Emslie E.A. Nature 359:644-647(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Foreskin.
[2]	"Isolation and characterization of a human dual specificity protein-tyrosine phosphatase gene." Kwak S.P., Hakes D.J., Martell K.J., Dixon J.E. J. Biol. Chem. 269:3596-3604(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[3]	NIEHS SNPs program Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS THR-56 AND HIS-187.
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
+	Additional computationally mapped references.

Web resources

NIEHS-SNPs

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases
EMBL GenBank DDBJ	X68277 mRNA. Translation: CAA48338.1. DQ301957 Genomic DNA. Translation: ABB96250.1. CH471062 Genomic DNA. Translation: EAW61425.1. CH471062 Genomic DNA. Translation: EAW61426.1. BC022463 mRNA. Translation: AAH22463.1.
IPI	IPI00003928.
PIR	S29090.
RefSeq	NP_004408.1. NM_004417.3.
UniGene	Hs.171695.
3D structure databases
ProteinModelPortal	P28562.
ModBase	Search...
Protein-protein interaction databases
IntAct	P28562. 10 interactions.
MINT	MINT-8217028.
STRING	9606.ENSP00000239223.
PTM databases
PhosphoSite	P28562.
Polymorphism databases
DMDM	1346900.
Proteomic databases
PaxDb	P28562.
PRIDE	P28562.
Protocols and materials databases
DNASU	1843.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000239223; ENSP00000239223; ENSG00000120129.
GeneID	1843.
KEGG	hsa:1843.
UCSC	uc003mbv.2. human.
Organism-specific databases
CTD	1843.
GeneCards	GC05M172195.
HGNC	HGNC:3064. DUSP1.
HPA	CAB018554.
MIM	600714. gene.
neXtProt	NX_P28562.
PharmGKB	PA27519.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG2453.
HOGENOM	HOG000294080.
HOVERGEN	HBG007347.
InParanoid	P28562.
KO	K04459.
OMA	VLDCRSF.
OrthoDB	EOG4T1HMT.
PhylomeDB	P28562.
Enzyme and pathway databases
Pathway_Interaction_DB	fcer1pathway. Fc-epsilon receptor I signaling in mast cells. p38alphabetapathway. Regulation of p38-alpha and p38-beta.
SignaLink	P28562.
Gene expression databases
ArrayExpress	P28562.
Bgee	P28562.
CleanEx	HS_DUSP1.
Genevestigator	P28562.
GermOnline	ENSG00000120129. Homo sapiens.
Family and domain databases
Gene3D	3.40.250.10. 1 hit.
InterPro	IPR020417. Atypical_DUSP. IPR000340. Dual-sp_phosphatase_cat-dom. IPR020422. Dual-sp_phosphatase_subgr_cat. IPR024950. DUSP. IPR008343. MKP. IPR001763. Rhodanese-like_dom. IPR000387. Tyr/Dual-sp_Pase. IPR016130. Tyr_Pase_AS. [Graphical view]
PANTHER	PTHR10159. PTHR10159. 1 hit.
Pfam	PF00782. DSPc. 1 hit. PF00581. Rhodanese. 1 hit. [Graphical view]
PIRSF	PIRSF000939. MAPK_Ptase. 1 hit.
PRINTS	PR01908. ADSPHPHTASE. PR01764. MAPKPHPHTASE.
SMART	SM00195. DSPc. 1 hit. SM00450. RHOD. 1 hit. [Graphical view]
SUPFAM	SSF52821. Rhodanese-like. 1 hit.
PROSITE	PS50206. RHODANESE_3. 1 hit. PS00383. TYR_PHOSPHATASE_1. 1 hit. PS50056. TYR_PHOSPHATASE_2. 1 hit. PS50054. TYR_PHOSPHATASE_DUAL. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P28562.
ChEMBL	CHEMBL6026.
ChiTaRS	DUSP1. human.
GenomeRNAi	1843.
NextBio	7547.
SOURCE	Search...

Entry information

Entry name

DUS1_HUMAN

Accession

Primary (citable) accession number: P28562
Secondary accession number(s): D3DQL9, Q2V508

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	February 1, 1996
Last modified:	May 29, 2013
This is version 132 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.