Reviewed, UniProtKB/Swiss-Prot P28548 (CSK2B_CAEEL)

Names and origin

Protein names

Recommended name:
    Casein kinase II subunit beta
      Short name=CK II beta

Gene names

Name:	kin-10
Synonyms:	kin-5
ORF Names:	T01G9.6

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	234 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Participates in Wnt signaling. Plays a complex role in regulating the basal catalytic activity of the alpha subunit By similarity. Modulates two aspects of male mating behavior; response to hermaphrodite contact and vulval location, acting in the same pathway as lov-1 and pkd-2.
Subunit structure	Tetramer of two alpha and two beta subunits. Interacts (via C-terminus) with lov-1 (via PLAT domain). Ref.3
Subcellular location	Cell projection › axon. Cell projection › cilium. Cell projection › dendrite. Perikaryon. Note: Enriched in cilia in male sensory neurons. Ref.3
Tissue specificity	Expressed in a subset of the adult male sensory neurons: CEM head neurons, ray RnB neurons, and hook HOB tail neurons. Ref.3
Developmental stage	Elevated levels are observed during embryogenesis, liver regeneration, and adipocyte differentiation.
Post-translational modification	Phosphorylated by alpha subunit By similarity.
Sequence similarities	Belongs to the casein kinase 2 subunit beta family.

Ontologies

Keywords
Biological process	Behavior Wnt signaling pathway
Cellular component	Cell projection Cilium
Coding sequence diversity	Alternative splicing
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW body morphogenesis Inferred from mutant phenotype. Source: WormBase embryonic development ending in birth or egg hatching Ref.3 Inferred from mutant phenotype. Source: WormBase growth Inferred from mutant phenotype. Source: WormBase hermaphrodite genitalia development Ref.3 Inferred from mutant phenotype. Source: WormBase locomotion Inferred from mutant phenotype. Source: WormBase morphogenesis of an epithelium Inferred from mutant phenotype. Source: WormBase nematode larval development Inferred from mutant phenotype. Source: WormBase positive regulation of growth rate Ref.3 Inferred from mutant phenotype. Source: WormBase response to hermaphrodite contact Ref.3 Inferred from mutant phenotype. Source: UniProtKB vulval location Ref.3 Inferred from mutant phenotype. Source: UniProtKB
Cellular component	axon Ref.3 Inferred from direct assay. Source: UniProtKB cilium Ref.3 Inferred from direct assay. Source: UniProtKB dendrite Ref.3 Inferred from direct assay. Source: UniProtKB perikaryon Inferred from electronic annotation. Source: UniProtKB-SubCell protein kinase CK2 complex Inferred from electronic annotation. Source: InterPro
Molecular function	protein binding Ref.3 Inferred from physical interaction. Source: UniProtKB protein kinase regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
atf-2	Q21361	1	EBI-317777,EBI-317743
eya-1	O17670	1	EBI-317777,EBI-311862
gei-16	Q22534	1	EBI-317777,EBI-327642
gei-4	Q9TZ38	1	EBI-317777,EBI-329192

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform a (identifier: P28548-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform b (identifier: P28548-2)

The sequence of this isoform differs from the canonical sequence as follows:
     58-58: P → PE

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 234	234	Casein kinase II subunit beta		PRO_0000068244
Regions
	Compositional bias	55 – 63	9	Asp/Glu-rich (acidic)
Amino acid modifications
	Modified residue	2	1	Phosphoserine; by autocatalysis Probable
Natural variations
	Alternative sequence	58	1	P → PE in isoform b.		VSP_001093
Experimental info
	Sequence conflict	141	1	D → M in AAA27983. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform a [UniParc]. Last modified July 22, 2008. Version 2. Checksum: 634E76B78761AD74	FASTA	234	26,436
	10 20 30 40 50 60 MSSSEEVSWI TWFCGLRGNE FFCEVDEEYI QDRFNLTGLN EQVPKYRQAL DMILDLEPDD 70 80 90 100 110 120 IEDNATNTDL VEQAAEMLYG LIHARYILTN RGISQMVEKW RDHDFGVCPR VYCENQPMLP 130 140 150 160 170 180 IGLSDVPGEA MVKLYCPRCN DVFVPRSSRH QHTDGSYFGT GFPHMLFFVH PDLRPRRPVT 190 200 210 220 230 QFVPKLYGFK IHPVAYGGQE GNSGGNTANN VAAAQNNTTP AGQQSGGQFN NYGL
	Isoform b. Checksum: 72B05EC0E954B05A	FASTA	235	26,565
	10 20 30 40 50 60 MSSSEEVSWI TWFCGLRGNE FFCEVDEEYI QDRFNLTGLN EQVPKYRQAL DMILDLEPED 70 80 90 100 110 120 DIEDNATNTD LVEQAAEMLY GLIHARYILT NRGISQMVEK WRDHDFGVCP RVYCENQPML 130 140 150 160 170 180 PIGLSDVPGE AMVKLYCPRC NDVFVPRSSR HQHTDGSYFG TGFPHMLFFV HPDLRPRRPV 190 200 210 220 230 TQFVPKLYGF KIHPVAYGGQ EGNSGGNTAN NVAAAQNNTT PAGQQSGGQF NNYGL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Casein kinase II from Caenorhabditis elegans. Cloning, characterization, and developmental regulation of the gene encoding the beta subunit." Hu E., Rubin C.S. J. Biol. Chem. 266:19796-19802(1991) [PubMed: 1918084] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM A).
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
[3]	"Casein kinase II and calcineurin modulate TRPP function and ciliary localization." Hu J., Bae Y.-K., Knobel K.M., Barr M.M. Mol. Biol. Cell 17:2200-2211(2006) [PubMed: 16481400] [Abstract] Cited for: FUNCTION, INTERACTION WITH LOV-1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M73827 Genomic DNA. Translation: AAA27983.1. Z75713 Genomic DNA. Translation: CAB00056.1. Z75713 Genomic DNA. Translation: CAB00053.1.
PIR	B87852. T24317.
RefSeq	NP_492255.1.
UniGene	Cel.17807
3D structure databases
HSSP	HSSP built from PDB template 1QF8 based on UniProtKB P13862.
SMR	P28548. Positions 2-196.
ModBase	Search...
Protein-protein interaction databases
IntAct	P28548. 7 interactions.
Proteomic databases
PRIDE	P28548.
Genome annotation databases
Ensembl	T01G9.6. Caenorhabditis elegans. [Contig view]
GeneID	172610.
Organism-specific databases
WormBase	WBGene00002196. kin-10.
WormPep	T01G9.6a. CE18168. [WorfDB] T01G9.6b. CE06343. [WorfDB]
Phylogenomic databases
OMA	P28548. FKIHPLA.
Gene expression databases
ArrayExpress	P28548.
Family and domain databases
InterPro	IPR016149. Casein_kin_II_reg-sub_a-hlx. IPR016150. Casein_kin_II_reg-sub_b-sht. IPR000704. Casein_kinase_II_reg-sub. [Graphical view]
Gene3D	G3DSA:1.10.1820.10. Casein_kin_II_reg-sub_a-hlx. 1 hit. G3DSA:2.20.25.20. Casein_kin_II_reg-sub_b-sht. 1 hit.
PANTHER	PTHR11740. CAS_kinase_II. 1 hit.
Pfam	PF01214. CK_II_beta. 1 hit. [Graphical view]
PRINTS	PR00472. CASNKINASEII.
ProDom	PD003829. CAS_kinase_II. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS01101. CK2_BETA. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	876245.

Entry information

Entry name

CSK2B_CAEEL

Accession

Primary (citable) accession number: P28548
Secondary accession number(s): O62352, Q22077

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	July 22, 2008
Last modified:	June 16, 2009
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project