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Protein

DNA repair protein RAD14

Gene

RAD14

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in nucleotide excision repair. Binds specifically to damaged DNA. Required for the incision step.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri191 – 21626Add
BLAST

GO - Molecular functioni

  • damaged DNA binding Source: SGD
  • zinc ion binding Source: SGD

GO - Biological processi

  • nucleotide-excision repair, DNA damage recognition Source: SGD
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32888-MONOMER.
ReactomeiR-SCE-5696395. Formation of Incision Complex in GG-NER.
R-SCE-5696400. Dual Incision in GG-NER.
R-SCE-6781823. Formation of TC-NER Pre-Incision Complex.
R-SCE-6782135. Dual incision in TC-NER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein RAD14
Gene namesi
Name:RAD14
Ordered Locus Names:YMR201C
ORF Names:YM8325.02C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR201C.
SGDiS000004814. RAD14.

Subcellular locationi

GO - Cellular componenti

  • nucleotide-excision repair factor 1 complex Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi207 – 2071V → M in RAD14-2; loss of recognition of cyclobutane pyrimidine dimers.
Mutagenesisi216 – 2161C → Y in RAD14-2; loss of recognition of cyclobutane pyrimidine dimers.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 371371DNA repair protein RAD14PRO_0000208654Add
BLAST

Proteomic databases

MaxQBiP28519.
PeptideAtlasiP28519.

PTM databases

iPTMnetiP28519.

Interactioni

Subunit structurei

Component of the nucleotide excision repair factor 1 (NEF1) complex consisting of RAD1, RAD10 and RAD14.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
RAD1P067773EBI-14641,EBI-14752

Protein-protein interaction databases

BioGridi35379. 143 interactions.
DIPiDIP-1896N.
IntActiP28519. 3 interactions.
MINTiMINT-406437.

Structurei

Secondary structure

1
371
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni192 – 1943Combined sources
Helixi202 – 2065Combined sources
Helixi214 – 2196Combined sources
Helixi221 – 2244Combined sources
Beta strandi226 – 2283Combined sources
Helixi229 – 2368Combined sources
Helixi240 – 2434Combined sources
Turni246 – 2483Combined sources
Beta strandi252 – 2543Combined sources
Beta strandi265 – 2695Combined sources
Helixi270 – 28112Combined sources
Helixi283 – 30018Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5A39X-ray2.80A/B188-302[»]
5A3DX-ray1.80A/B188-302[»]
ProteinModelPortaliP28519.
SMRiP28519. Positions 188-302.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the XPA family.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri191 – 21626Add
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00390000002721.
HOGENOMiHOG000161510.
InParanoidiP28519.
KOiK10847.
OMAiCHINIEM.
OrthoDBiEOG7H79F8.

Family and domain databases

Gene3Di3.90.530.10. 1 hit.
InterProiIPR009061. DNA-bd_dom_put.
IPR000465. XPA.
IPR022656. XPA_C.
IPR022658. XPA_CS.
IPR022652. Znf_XPA_CS.
[Graphical view]
PANTHERiPTHR10142:SF0. PTHR10142:SF0. 2 hits.
PfamiPF05181. XPA_C. 1 hit.
[Graphical view]
SUPFAMiSSF46955. SSF46955. 1 hit.
TIGRFAMsiTIGR00598. rad14. 1 hit.
PROSITEiPS00752. XPA_1. 1 hit.
PS00753. XPA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28519-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPEQKAKLE ANRKLAIERL RKRGILSSDQ LNRIESRNEP LKTRPLAVTS
60 70 80 90 100
GSNRDDNAAA AVHVPNHNGQ PSALANTNTN TTSLYGSGVV DGSKRDASVL
110 120 130 140 150
DKRPTDRIRP SIRKQDYIEY DFATMQNLNG GYINPKDKLP NSDFTDDQEF
160 170 180 190 200
ESEFGSKKQK TLQDWKKEQL ERKMLYENAP PPEHISKAPK CIECHINIEM
210 220 230 240 250
DPVLHDVFKL QVCKQCSKEH PEKYALLTKT ECKEDYFLTD PELNDEDLFH
260 270 280 290 300
RLEKPNPHSG TFARMQLFVR CEVEAFAFKK WGGEEGLDEE WQRREEGKAH
310 320 330 340 350
RREKKYEKKI KEMRLKTRAQ EYTNRLREKK HGKAHIHHFS DPVDGGIDED
360 370
GYQIQRRRCT DCGLETEEID I
Length:371
Mass (Da):43,038
Last modified:February 1, 1996 - v2
Checksum:iA90FA9CAA6F3EA52
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64064 Genomic DNA. Translation: CAA45420.1.
Z48755 Genomic DNA. Translation: CAA88642.1.
BK006946 Genomic DNA. Translation: DAA10100.1.
PIRiS59442.
RefSeqiNP_013928.1. NM_001182708.1.

Genome annotation databases

EnsemblFungiiYMR201C; YMR201C; YMR201C.
GeneIDi855241.
KEGGisce:YMR201C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64064 Genomic DNA. Translation: CAA45420.1.
Z48755 Genomic DNA. Translation: CAA88642.1.
BK006946 Genomic DNA. Translation: DAA10100.1.
PIRiS59442.
RefSeqiNP_013928.1. NM_001182708.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5A39X-ray2.80A/B188-302[»]
5A3DX-ray1.80A/B188-302[»]
ProteinModelPortaliP28519.
SMRiP28519. Positions 188-302.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35379. 143 interactions.
DIPiDIP-1896N.
IntActiP28519. 3 interactions.
MINTiMINT-406437.

PTM databases

iPTMnetiP28519.

Proteomic databases

MaxQBiP28519.
PeptideAtlasiP28519.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR201C; YMR201C; YMR201C.
GeneIDi855241.
KEGGisce:YMR201C.

Organism-specific databases

EuPathDBiFungiDB:YMR201C.
SGDiS000004814. RAD14.

Phylogenomic databases

GeneTreeiENSGT00390000002721.
HOGENOMiHOG000161510.
InParanoidiP28519.
KOiK10847.
OMAiCHINIEM.
OrthoDBiEOG7H79F8.

Enzyme and pathway databases

BioCyciYEAST:G3O-32888-MONOMER.
ReactomeiR-SCE-5696395. Formation of Incision Complex in GG-NER.
R-SCE-5696400. Dual Incision in GG-NER.
R-SCE-6781823. Formation of TC-NER Pre-Incision Complex.
R-SCE-6782135. Dual incision in TC-NER.

Miscellaneous databases

PROiP28519.

Family and domain databases

Gene3Di3.90.530.10. 1 hit.
InterProiIPR009061. DNA-bd_dom_put.
IPR000465. XPA.
IPR022656. XPA_C.
IPR022658. XPA_CS.
IPR022652. Znf_XPA_CS.
[Graphical view]
PANTHERiPTHR10142:SF0. PTHR10142:SF0. 2 hits.
PfamiPF05181. XPA_C. 1 hit.
[Graphical view]
SUPFAMiSSF46955. SSF46955. 1 hit.
TIGRFAMsiTIGR00598. rad14. 1 hit.
PROSITEiPS00752. XPA_1. 1 hit.
PS00753. XPA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the rad14-2 mutant of Saccharomyces cerevisiae: implications for the recognition of UV photoproducts by the Rad14 protein."
    Jones G.W., Reed S.H., Waters R.
    Yeast 13:31-36(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTANT RAD14-2.
  2. "Yeast RAD14 and human Xeroderma pigmentosum group A DNA-repair genes encode homologous proteins."
    Bankmann M., Prakash L., Prakash S.
    Nature 355:555-558(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 125-371.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Yeast DNA-repair gene RAD14 encodes a zinc metalloprotein with affinity for ultraviolet-damaged DNA."
    Guzder S.N., Sung P., Prakash L., Prakash S.
    Proc. Natl. Acad. Sci. U.S.A. 90:5433-5437(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. "Nucleotide excision repair in yeast is mediated by sequential assembly of repair factors and not by a pre-assembled repairosome."
    Guzder S.N., Sung P., Prakash L., Prakash S.
    J. Biol. Chem. 271:8903-8910(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE NEF1 COMPLEX.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRAD14_YEAST
AccessioniPrimary (citable) accession number: P28519
Secondary accession number(s): D6W026
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: February 1, 1996
Last modified: June 8, 2016
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1030 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.