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Protein

DNA repair protein RAD14

Gene

RAD14

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in nucleotide excision repair. Binds specifically to damaged DNA. Required for the incision step.

Miscellaneous

Present with 1030 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri191 – 216Add BLAST26

GO - Molecular functioni

  • damaged DNA binding Source: SGD
  • zinc ion binding Source: SGD

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processDNA damage, DNA repair
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32888-MONOMER.
ReactomeiR-SCE-5696395. Formation of Incision Complex in GG-NER.
R-SCE-5696400. Dual Incision in GG-NER.
R-SCE-6781823. Formation of TC-NER Pre-Incision Complex.
R-SCE-6782135. Dual incision in TC-NER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein RAD14
Gene namesi
Name:RAD14
Ordered Locus Names:YMR201C
ORF Names:YM8325.02C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR201C.
SGDiS000004814. RAD14.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi207V → M in RAD14-2; loss of recognition of cyclobutane pyrimidine dimers. 1
Mutagenesisi216C → Y in RAD14-2; loss of recognition of cyclobutane pyrimidine dimers. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002086541 – 371DNA repair protein RAD14Add BLAST371

Proteomic databases

MaxQBiP28519.
PRIDEiP28519.

PTM databases

iPTMnetiP28519.

Interactioni

Subunit structurei

Component of the nucleotide excision repair factor 1 (NEF1) complex consisting of RAD1, RAD10 and RAD14.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
RAD1P067774EBI-14641,EBI-14752

Protein-protein interaction databases

BioGridi35379. 370 interactors.
DIPiDIP-1896N.
ELMiP28519.
IntActiP28519. 10 interactors.
MINTiMINT-406437.
STRINGi4932.YMR201C.

Structurei

Secondary structure

1371
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni192 – 194Combined sources3
Helixi202 – 206Combined sources5
Helixi214 – 219Combined sources6
Helixi221 – 224Combined sources4
Beta strandi226 – 228Combined sources3
Helixi229 – 236Combined sources8
Helixi240 – 243Combined sources4
Turni246 – 248Combined sources3
Beta strandi252 – 254Combined sources3
Beta strandi265 – 269Combined sources5
Helixi270 – 281Combined sources12
Helixi283 – 300Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5A39X-ray2.80A/B188-302[»]
5A3DX-ray1.80A/B188-302[»]
5G32X-ray2.20A/B188-306[»]
5G33X-ray2.40A/B188-306[»]
5G34X-ray1.90A/B188-306[»]
5G35X-ray2.00A/B188-306[»]
5LCLX-ray2.20A188-306[»]
B1-371[»]
5LCMX-ray1.90A/B188-306[»]
ProteinModelPortaliP28519.
SMRiP28519.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the XPA family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri191 – 216Add BLAST26

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00390000002721.
HOGENOMiHOG000161510.
InParanoidiP28519.
KOiK10847.
OMAiCHINIEM.
OrthoDBiEOG092C44LK.

Family and domain databases

Gene3Di3.90.530.10. 1 hit.
InterProiView protein in InterPro
IPR009061. DNA-bd_dom_put_sf.
IPR000465. XPA.
IPR022656. XPA_C.
IPR037129. XPA_C_sf.
IPR022658. XPA_CS.
IPR022652. Znf_XPA_CS.
PANTHERiPTHR10142. PTHR10142. 1 hit.
PfamiView protein in Pfam
PF05181. XPA_C. 1 hit.
SUPFAMiSSF46955. SSF46955. 1 hit.
TIGRFAMsiTIGR00598. rad14. 1 hit.
PROSITEiView protein in PROSITE
PS00752. XPA_1. 1 hit.
PS00753. XPA_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P28519-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPEQKAKLE ANRKLAIERL RKRGILSSDQ LNRIESRNEP LKTRPLAVTS
60 70 80 90 100
GSNRDDNAAA AVHVPNHNGQ PSALANTNTN TTSLYGSGVV DGSKRDASVL
110 120 130 140 150
DKRPTDRIRP SIRKQDYIEY DFATMQNLNG GYINPKDKLP NSDFTDDQEF
160 170 180 190 200
ESEFGSKKQK TLQDWKKEQL ERKMLYENAP PPEHISKAPK CIECHINIEM
210 220 230 240 250
DPVLHDVFKL QVCKQCSKEH PEKYALLTKT ECKEDYFLTD PELNDEDLFH
260 270 280 290 300
RLEKPNPHSG TFARMQLFVR CEVEAFAFKK WGGEEGLDEE WQRREEGKAH
310 320 330 340 350
RREKKYEKKI KEMRLKTRAQ EYTNRLREKK HGKAHIHHFS DPVDGGIDED
360 370
GYQIQRRRCT DCGLETEEID I
Length:371
Mass (Da):43,038
Last modified:February 1, 1996 - v2
Checksum:iA90FA9CAA6F3EA52
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64064 Genomic DNA. Translation: CAA45420.1.
Z48755 Genomic DNA. Translation: CAA88642.1.
BK006946 Genomic DNA. Translation: DAA10100.1.
PIRiS59442.
RefSeqiNP_013928.1. NM_001182708.1.

Genome annotation databases

EnsemblFungiiYMR201C; YMR201C; YMR201C.
GeneIDi855241.
KEGGisce:YMR201C.

Similar proteinsi

Entry informationi

Entry nameiRAD14_YEAST
AccessioniPrimary (citable) accession number: P28519
Secondary accession number(s): D6W026
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: February 1, 1996
Last modified: November 22, 2017
This is version 148 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names