P28519 (RAD14_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA repair protein RAD14

Gene names

Name:	RAD14
Ordered Locus Names:	YMR201C
ORF Names:	YM8325.02C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	371 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in nucleotide excision repair. Binds specifically to damaged DNA. Required for the incision step.
Subunit structure	Component of the nucleotide excision repair factor 1 (NEF1) complex consisting of RAD1, RAD10 and RAD14.
Subcellular location	Nucleus.
Miscellaneous	Present with 1030 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Belongs to the XPA family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nucleotide-excision repair, DNA damage recognition Inferred from direct assay Ref.5. Source: SGD
Cellular component	nucleotide-excision repair factor 1 complex Traceable author statement. Source: SGD
Molecular function	damaged DNA binding Inferred from direct assay Ref.5. Source: SGD nucleotide binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct zinc ion binding Inferred from direct assay Ref.5. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RAD1	P06777	4	EBI-14641,EBI-14752

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 371	371	DNA repair protein RAD14		PRO_0000208654
Regions
	Zinc finger	191 – 216	26
Experimental info
	Mutagenesis	207	1	V → M in RAD14-2; loss of recognition of cyclobutane pyrimidine dimers.
	Mutagenesis	216	1	C → Y in RAD14-2; loss of recognition of cyclobutane pyrimidine dimers.

Sequences

	Sequence		Length	Mass (Da)
	P28519 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: A90FA9CAA6F3EA52	FASTA	371	43,038
	10 20 30 40 50 60 MTPEQKAKLE ANRKLAIERL RKRGILSSDQ LNRIESRNEP LKTRPLAVTS GSNRDDNAAA 70 80 90 100 110 120 AVHVPNHNGQ PSALANTNTN TTSLYGSGVV DGSKRDASVL DKRPTDRIRP SIRKQDYIEY 130 140 150 160 170 180 DFATMQNLNG GYINPKDKLP NSDFTDDQEF ESEFGSKKQK TLQDWKKEQL ERKMLYENAP 190 200 210 220 230 240 PPEHISKAPK CIECHINIEM DPVLHDVFKL QVCKQCSKEH PEKYALLTKT ECKEDYFLTD 250 260 270 280 290 300 PELNDEDLFH RLEKPNPHSG TFARMQLFVR CEVEAFAFKK WGGEEGLDEE WQRREEGKAH 310 320 330 340 350 360 RREKKYEKKI KEMRLKTRAQ EYTNRLREKK HGKAHIHHFS DPVDGGIDED GYQIQRRRCT 370 DCGLETEEID I

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the rad14-2 mutant of Saccharomyces cerevisiae: implications for the recognition of UV photoproducts by the Rad14 protein." Jones G.W., Reed S.H., Waters R. Yeast 13:31-36(1997) [PubMed: 9046084] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTANT RAD14-2.
[2]	"Yeast RAD14 and human Xeroderma pigmentosum group A DNA-repair genes encode homologous proteins." Bankmann M., Prakash L., Prakash S. Nature 355:555-558(1992) [PubMed: 1741034] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 125-371.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Yeast DNA-repair gene RAD14 encodes a zinc metalloprotein with affinity for ultraviolet-damaged DNA." Guzder S.N., Sung P., Prakash L., Prakash S. Proc. Natl. Acad. Sci. U.S.A. 90:5433-5437(1993) [PubMed: 8516285] [Abstract] Cited for: CHARACTERIZATION.
[6]	"Nucleotide excision repair in yeast is mediated by sequential assembly of repair factors and not by a pre-assembled repairosome." Guzder S.N., Sung P., Prakash L., Prakash S. J. Biol. Chem. 271:8903-8910(1996) [PubMed: 8621533] [Abstract] Cited for: IDENTIFICATION IN THE NEF1 COMPLEX.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X64064 Genomic DNA. Translation: CAA45420.1. Z48755 Genomic DNA. Translation: CAA88642.1. BK006946 Genomic DNA. Translation: DAA10100.1.
PIR	S59442.
RefSeq	NP_013928.1. NM_001182708.1.
3D structure databases
ProteinModelPortal	P28519.
SMR	P28519. Positions 188-297.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1896N.
IntAct	P28519. 7 interactions.
MINT	MINT-406437.
STRING	P28519.
Proteomic databases
PeptideAtlas	P28519.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR201C; YMR201C; YMR201C.
GeneID	855241.
KEGG	sce:YMR201C.
NMPDR	fig|4932.3.peg.4981.
Organism-specific databases
CYGD	YMR201c.
SGD	S000004814. RAD14.
Phylogenomic databases
eggNOG	fuNOG05450.
GeneTree	EFGT00050000006725.
HOGENOM	HBG738603.
OMA	ECKEDYF.
OrthoDB	EOG44N22D.
Gene expression databases
ArrayExpress	P28519.
Genevestigator	P28519.
GermOnline	YMR201C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR009061. DNA-bd_dom_put. IPR000465. XPA. IPR022656. XPA_C. IPR022658. XPA_CS. IPR022652. Znf_XPA_CS. [Graphical view]
KO	K10847.
PANTHER	PTHR10142. XPA. 1 hit.
Pfam	PF05181. XPA_C. 1 hit. [Graphical view]
SUPFAM	SSF46955. Putativ_DNA_bind. 1 hit.
TIGRFAMs	TIGR00598. Rad14. 1 hit.
PROSITE	PS00752. XPA_1. 1 hit. PS00753. XPA_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	978795.

Entry information

Entry name

RAD14_YEAST

Accession

Primary (citable) accession number: P28519
Secondary accession number(s): D6W026

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	February 1, 1996
Last modified:	December 14, 2011
This is version 99 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program