P28512 (HIRP6_HIRMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Hirudin-P6
Organism	Hirudinaria manillensis (Buffalo leech) (Mexican medical leech)
Taxonomic identifier	6419 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Annelida › Clitellata › Hirudinida › Hirudinea › Arhynchobdellida › Hirudiniformes › Hirudinidae › Hirudinaria

Protein attributes

Sequence length	63 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hirudin is a potent thrombin-specific protease inhibitor. It forms a stable non-covalent complex with alpha-thrombin, thereby abolishing its ability to cleave fibrinogen.
Subcellular location	Secreted.
Post-translational modification	O-linked glycan consists of Fuc-Gal-GalNAc trisaccharide.
Sequence similarities	Belongs to the protease inhibitor I14 (hirudin) family. [View classification]

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Protease inhibitor Serine protease inhibitor
PTM	Disulfide bond Glycoprotein Sulfation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 63	63	Hirudin-P6		PRO_0000195652
Regions
	Region	1 – 3	3	Interaction with thrombin active site By similarity
	Region	53 – 63	11	Interaction with fibrinogen-binding exosite of thrombin By similarity
Amino acid modifications
	Modified residue	61	1	Sulfotyrosine Ref.1
	Glycosylation	43	1	O-linked (GalNAc...)		CAR_000143
	Disulfide bond	6 ↔ 14		By similarity
	Disulfide bond	16 ↔ 28		By similarity
	Disulfide bond	22 ↔ 37		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P28512 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 149A7369CC75A192	FASTA	63	6,977
	10 20 30 40 50 60 MRYTACTESG QNQCICEGND VCGQGRNCQF DSSGKKCVEG EGTRKPQNEG QHDFDPIPEE YLS

References

[1]	"Primary structure and function of novel O-glycosylated hirudins from the leech Hirudinaria manillensis." Steiner V., Knecht R., Boernsen O., Gassmann E., Stone S.R., Raschdorf F., Schlaeppi J.-M., Maschler R. Biochemistry 31:2294-2298(1992) [PubMed: 1540584] [Abstract] Cited for: PROTEIN SEQUENCE, STRUCTURE OF CARBOHYDRATE, SULFATION AT TYR-61, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
PIR	A42207.
3D structure databases
ProteinModelPortal	P28512.
SMR	P28512. Positions 3-62.
ModBase	Search...
PTM databases
GlycoSuiteDB	P28512.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000429. Prot_inh_hirudin. IPR011061. Prot_inh_I14/15_hirudin/antisn. [Graphical view]
Pfam	PF00713. Hirudin. 1 hit. [Graphical view]
PIRSF	PIRSF001640. Hirudin. 1 hit.
PRINTS	PR00777. HIRUDIN.
ProDom	PD004216. Prot_inh_hirudin. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF57262. Antihaemostatic. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HIRP6_HIRMA

Accession

Primary (citable) accession number: P28512

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	December 14, 2011
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)