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Protein

Hirudin-3B'

Gene
N/A
Organism
Hirudo medicinalis (Medicinal leech)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Hirudin is a potent thrombin-specific protease inhibitor. It forms a stable non-covalent complex with alpha-thrombin, thereby abolishing its ability to cleave fibrinogen.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI14.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Hirudin-3B'
Alternative name(s):
Hirudin IIIB'
OrganismiHirudo medicinalis (Medicinal leech)
Taxonomic identifieri6421 [NCBI]
Taxonomic lineageiEukaryotaMetazoaLophotrochozoaAnnelidaClitellataHirudinidaHirudineaArhynchobdellidaHirudiniformesHirudinidaeHirudo

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Protein family/group databases

Allergomei9843. Hir me Hirudin.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001956511 – 65Hirudin-3B'Add BLAST65

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi6 ↔ 14By similarity
Disulfide bondi16 ↔ 28By similarity
Disulfide bondi22 ↔ 39By similarity
Glycosylationi45O-linked (GalNAc...)By similarity1
Modified residuei63SulfotyrosineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Sulfation

Structurei

Secondary structure

165
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi61 – 63Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1D6WX-ray2.00I55-64[»]
1O5GX-ray1.75I55-65[»]
1Z71X-ray1.80B55-65[»]
1ZGIX-ray2.20B55-64[»]
1ZGVX-ray2.20B55-64[»]
2BDYX-ray1.61B55-64[»]
3C1KX-ray1.84B55-65[»]
5AFYX-ray1.12I54-65[»]
5AFZX-ray1.53I54-65[»]
5AHGX-ray1.24I54-65[»]
ProteinModelPortaliP28511.
SMRiP28511.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP28511.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 3Interaction with thrombin active siteBy similarity3
Regioni55 – 65Interaction with fibrinogen-binding exosite of thrombinBy similarityAdd BLAST11

Sequence similaritiesi

Family and domain databases

Gene3Di2.70.10.10. 1 hit.
InterProiIPR024793. Hirudin.
IPR011061. Hirudin/antistatin.
IPR000429. Prot_inh_hirudin.
[Graphical view]
PfamiPF00713. Hirudin. 1 hit.
[Graphical view]
PIRSFiPIRSF001640. Hirudin. 1 hit.
PRINTSiPR00777. HIRUDIN.
ProDomiPD004216. Prot_inh_hirudin. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF57262. SSF57262. 1 hit.

Sequencei

Sequence statusi: Complete.

P28511-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
VVYTDCTESG QNLCLCQGSN VCGQGNKCIL GSNGEKNQCV TGEGTPKPQS
60
HNDGDFEEIP EEYLQ
Length:65
Mass (Da):6,968
Last modified:December 1, 1992 - v1
Checksum:i85FD9E11AE3DE9FF
GO

Sequence databases

PIRiS78521.

Cross-referencesi

Sequence databases

PIRiS78521.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1D6WX-ray2.00I55-64[»]
1O5GX-ray1.75I55-65[»]
1Z71X-ray1.80B55-65[»]
1ZGIX-ray2.20B55-64[»]
1ZGVX-ray2.20B55-64[»]
2BDYX-ray1.61B55-64[»]
3C1KX-ray1.84B55-65[»]
5AFYX-ray1.12I54-65[»]
5AFZX-ray1.53I54-65[»]
5AHGX-ray1.24I54-65[»]
ProteinModelPortaliP28511.
SMRiP28511.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei9843. Hir me Hirudin.
MEROPSiI14.001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP28511.

Family and domain databases

Gene3Di2.70.10.10. 1 hit.
InterProiIPR024793. Hirudin.
IPR011061. Hirudin/antistatin.
IPR000429. Prot_inh_hirudin.
[Graphical view]
PfamiPF00713. Hirudin. 1 hit.
[Graphical view]
PIRSFiPIRSF001640. Hirudin. 1 hit.
PRINTSiPR00777. HIRUDIN.
ProDomiPD004216. Prot_inh_hirudin. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF57262. SSF57262. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHIR3C_HIRME
AccessioniPrimary (citable) accession number: P28511
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: November 2, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.