P28507 (HIR3A_HIRME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Hirudin-3A Alternative name(s): Hirudin IIIA
Organism	Hirudo medicinalis (Medicinal leech)
Taxonomic identifier	6421 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Lophotrochozoa › Annelida › Clitellata › Hirudinida › Hirudinea › Arhynchobdellida › Hirudiniformes › Hirudinidae › Hirudo

Protein attributes

Sequence length	65 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hirudin is a potent thrombin-specific protease inhibitor. It forms a stable non-covalent complex with alpha-thrombin, thereby abolishing its ability to cleave fibrinogen.
Subcellular location	Secreted.
Sequence similarities	Belongs to the protease inhibitor I14 (hirudin) family. [View classification]

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Protease inhibitor Serine protease inhibitor
PTM	Disulfide bond Glycoprotein Sulfation
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	serine-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 65

65

Hirudin-3A

PRO_0000195647

Regions

Region

1 – 3

3

Interaction with thrombin active site By similarity

Region

55 – 65

11

Interaction with fibrinogen-binding exosite of thrombin By similarity

Amino acid modifications

Modified residue

63

1

Sulfotyrosine By similarity

Glycosylation

45

1

O-linked (GalNAc...) By similarity

Disulfide bond

6 ↔ 14

By similarity

Disulfide bond

16 ↔ 28

By similarity

Disulfide bond

22 ↔ 39

By similarity

Secondary structure

1

.

.

.

65

Helix Strand Turn

Details...

Helix

61 – 63

3

Sequences

	Sequence		Length	Mass (Da)
	P28507 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 05F6A0B26A3DE9FC	FASTA	65	7,028
	10 20 30 40 50 60 VVYTDCTESG QNLCLCEDSN VCGEGNKCIL GSNGEKNQCV TGEGTPKPQS HNDGDFEEIP EEYLQ

References

[1]	"Primary structures of new 'iso-hirudins'." Scharf M., Engels J., Tripier D. FEBS Lett. 255:105-110(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE.
+	Additional computationally mapped references.

Cross-references

Sequence databases

PIR

S05675.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BMM	X-ray	2.60	I	54-65	[»]
1BMN	X-ray	2.80	I	54-65	[»]
1T4U	X-ray	2.00	I	55-65	[»]
1T4V	X-ray	2.00	I	55-65	[»]
1TOM	X-ray	1.80	I	55-64	[»]
2JH0	X-ray	1.70	H	55-64	[»]
2JH5	X-ray	2.50	H	55-64	[»]
2JH6	X-ray	2.21	H	55-64	[»]
3C27	X-ray	2.18	H	55-65	[»]

ProteinModelPortal

P28507.

SMR

P28507. Positions 1-65.

ModBase

Search...

Protein family/group databases

Allergome

9843. Hir me Hirudin.

MEROPS

I14.001.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

2.70.10.10. 1 hit.

InterPro

IPR024793. Hirudin.
IPR000429. Prot_inh_hirudin.
IPR011061. Prot_inh_I14/15_hirudin/antisn.
[Graphical view]

Pfam

PF00713. Hirudin. 1 hit.
[Graphical view]

PIRSF

PIRSF001640. Hirudin. 1 hit.

PRINTS

PR00777. HIRUDIN.

ProDom

PD004216. Prot_inh_hirudin. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SUPFAM

SSF57262. Antihaemostatic. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P28507.

Entry information

Entry name

HIR3A_HIRME

Accession

Primary (citable) accession number: P28507

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	April 3, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)