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Protein

Hirudin-2

Gene
N/A
Organism
Hirudo medicinalis (Medicinal leech)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Hirudin is a potent thrombin-specific protease inhibitor. It forms a stable non-covalent complex with alpha-thrombin, thereby abolishing its ability to cleave fibrinogen.

GO - Molecular functioni

  1. serine-type endopeptidase inhibitor activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI14.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Hirudin-2
Alternative name(s):
Hirudin II
OrganismiHirudo medicinalis (Medicinal leech)
Taxonomic identifieri6421 [NCBI]
Taxonomic lineageiEukaryotaMetazoaLophotrochozoaAnnelidaClitellataHirudinidaHirudineaArhynchobdellidaHirudiniformesHirudinidaeHirudo

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Protein family/group databases

Allergomei9843. Hir me Hirudin.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6565Hirudin-2PRO_0000195644Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi6 ↔ 14By similarity
Disulfide bondi16 ↔ 28By similarity
Disulfide bondi22 ↔ 39By similarity
Glycosylationi45 – 451O-linked (GalNAc...)By similarity
Modified residuei63 – 631SulfotyrosineBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Sulfation

Structurei

Secondary structure

1
65
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi61 – 633Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1A3BX-ray1.80I49-64[»]
1A3EX-ray1.85I49-64[»]
1ABIX-ray2.30I54-64[»]
1C1UX-ray1.75I55-65[»]
1C1VX-ray1.98I55-65[»]
1C1WX-ray1.90I55-65[»]
1C4UX-ray2.10355-63[»]
1C5LX-ray1.47I55-64[»]
1C5NX-ray1.50I55-64[»]
1C5OX-ray1.90I55-65[»]
1D9IX-ray2.30I55-64[»]
1FPCX-ray2.30I54-64[»]
1GHVX-ray1.85I55-65[»]
1GHWX-ray1.75I55-65[»]
1GHXX-ray1.65I55-65[»]
1GHYX-ray1.85I55-65[»]
1GJ4X-ray1.81I55-65[»]
1GJ5X-ray1.73I55-65[»]
1IHSX-ray2.00I49-65[»]
1NM6X-ray1.80B55-64[»]
1NT1X-ray2.00H55-64[»]
1NY2X-ray2.30355-64[»]
1O2GX-ray1.58I55-65[»]
1QBVX-ray1.80E55-65[»]
1SB1X-ray1.90I55-63[»]
1SL3X-ray1.81B55-64[»]
1TA2X-ray2.30B55-64[»]
1TA6X-ray1.90B55-64[»]
1TMTX-ray2.20J53-65[»]
1TWXX-ray2.40C55-64[»]
1VR1X-ray1.90I55-64[»]
1XM1X-ray2.30H55-64[»]
1YPEX-ray1.81I55-64[»]
1YPGX-ray1.80I55-64[»]
1YPJX-ray1.78I55-64[»]
1YPKX-ray1.78I55-64[»]
1YPLX-ray1.85I55-64[»]
1YPMX-ray1.85I55-64[»]
1ZRBX-ray1.90B55-65[»]
2GDEX-ray2.00D55-64[»]
2HGTX-ray2.20J54-64[»]
2PKSX-ray2.50D55-64[»]
3VXEX-ray1.25J54-64[»]
3VXFOther1.60J54-64[»]
ProteinModelPortaliP28504.
SMRiP28504. Positions 1-65.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP28504.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 33Interaction with thrombin active siteBy similarity
Regioni55 – 6511Interaction with fibrinogen-binding exosite of thrombinBy similarityAdd
BLAST

Sequence similaritiesi

Family and domain databases

Gene3Di2.70.10.10. 1 hit.
InterProiIPR024793. Hirudin.
IPR011061. Hirudin/antistatin.
IPR000429. Prot_inh_hirudin.
[Graphical view]
PfamiPF00713. Hirudin. 1 hit.
[Graphical view]
PIRSFiPIRSF001640. Hirudin. 1 hit.
PRINTSiPR00777. HIRUDIN.
ProDomiPD004216. Prot_inh_hirudin. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF57262. SSF57262. 1 hit.

Sequencei

Sequence statusi: Complete.

P28504-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
ITYTDCTESG QDLCLCEGSN VCGKGNKCIL GSNGEENQCV TGEGTPKPQS
60
HNDGDFEEIP EEYLQ
Length:65
Mass (Da):6,987
Last modified:December 1, 1992 - v1
Checksum:i757EEAE55EAEB27E
GO

Sequence databases

PIRiS05678.

Cross-referencesi

Sequence databases

PIRiS05678.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1A3BX-ray1.80I49-64[»]
1A3EX-ray1.85I49-64[»]
1ABIX-ray2.30I54-64[»]
1C1UX-ray1.75I55-65[»]
1C1VX-ray1.98I55-65[»]
1C1WX-ray1.90I55-65[»]
1C4UX-ray2.10355-63[»]
1C5LX-ray1.47I55-64[»]
1C5NX-ray1.50I55-64[»]
1C5OX-ray1.90I55-65[»]
1D9IX-ray2.30I55-64[»]
1FPCX-ray2.30I54-64[»]
1GHVX-ray1.85I55-65[»]
1GHWX-ray1.75I55-65[»]
1GHXX-ray1.65I55-65[»]
1GHYX-ray1.85I55-65[»]
1GJ4X-ray1.81I55-65[»]
1GJ5X-ray1.73I55-65[»]
1IHSX-ray2.00I49-65[»]
1NM6X-ray1.80B55-64[»]
1NT1X-ray2.00H55-64[»]
1NY2X-ray2.30355-64[»]
1O2GX-ray1.58I55-65[»]
1QBVX-ray1.80E55-65[»]
1SB1X-ray1.90I55-63[»]
1SL3X-ray1.81B55-64[»]
1TA2X-ray2.30B55-64[»]
1TA6X-ray1.90B55-64[»]
1TMTX-ray2.20J53-65[»]
1TWXX-ray2.40C55-64[»]
1VR1X-ray1.90I55-64[»]
1XM1X-ray2.30H55-64[»]
1YPEX-ray1.81I55-64[»]
1YPGX-ray1.80I55-64[»]
1YPJX-ray1.78I55-64[»]
1YPKX-ray1.78I55-64[»]
1YPLX-ray1.85I55-64[»]
1YPMX-ray1.85I55-64[»]
1ZRBX-ray1.90B55-65[»]
2GDEX-ray2.00D55-64[»]
2HGTX-ray2.20J54-64[»]
2PKSX-ray2.50D55-64[»]
3VXEX-ray1.25J54-64[»]
3VXFOther1.60J54-64[»]
ProteinModelPortaliP28504.
SMRiP28504. Positions 1-65.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei9843. Hir me Hirudin.
MEROPSiI14.001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP28504.

Family and domain databases

Gene3Di2.70.10.10. 1 hit.
InterProiIPR024793. Hirudin.
IPR011061. Hirudin/antistatin.
IPR000429. Prot_inh_hirudin.
[Graphical view]
PfamiPF00713. Hirudin. 1 hit.
[Graphical view]
PIRSFiPIRSF001640. Hirudin. 1 hit.
PRINTSiPR00777. HIRUDIN.
ProDomiPD004216. Prot_inh_hirudin. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF57262. SSF57262. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Primary structures of new 'iso-hirudins'."
    Scharf M., Engels J., Tripier D.
    FEBS Lett. 255:105-110(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.

Entry informationi

Entry nameiHIR2_HIRME
AccessioniPrimary (citable) accession number: P28504
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: January 7, 2015
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.