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P28504 (HIR2_HIRME) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Hirudin-2
Alternative name(s):
Hirudin II
OrganismHirudo medicinalis (Medicinal leech)
Taxonomic identifier6421 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Lophotrochozoa › Annelida › Clitellata › Hirudinida › Hirudinea › Arhynchobdellida › Hirudiniformes › Hirudinidae › Hirudo

Protein attributes

Sequence length65 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hirudin is a potent thrombin-specific protease inhibitor. It forms a stable non-covalent complex with alpha-thrombin, thereby abolishing its ability to cleave fibrinogen.

Subcellular location

Secreted.

Sequence similarities

Belongs to the protease inhibitor I14 (hirudin) family. [View classification]

Ontologies

Keywords
   Cellular componentSecreted
   Molecular functionProtease inhibitor
Serine protease inhibitor
   PTMDisulfide bond
Glycoprotein
Sulfation
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionserine-type endopeptidase inhibitor activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 6565Hirudin-2
PRO_0000195644

Regions

Region1 – 33Interaction with thrombin active site By similarity
Region55 – 6511Interaction with fibrinogen-binding exosite of thrombin By similarity

Amino acid modifications

Modified residue631Sulfotyrosine By similarity
Glycosylation451O-linked (GalNAc...) By similarity
Disulfide bond6 ↔ 14 By similarity
Disulfide bond16 ↔ 28 By similarity
Disulfide bond22 ↔ 39 By similarity

Secondary structure

1 ... 65
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P28504 [UniParc].

Last modified December 1, 1992. Version 1.
Checksum: 757EEAE55EAEB27E

FASTA656,987
        10         20         30         40         50         60 
ITYTDCTESG QDLCLCEGSN VCGKGNKCIL GSNGEENQCV TGEGTPKPQS HNDGDFEEIP 


EEYLQ

« Hide

References

[1]"Primary structures of new 'iso-hirudins'."
Scharf M., Engels J., Tripier D.
FEBS Lett. 255:105-110(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE.
+Additional computationally mapped references.

Cross-references

Sequence databases

PIRS05678.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1A3BX-ray1.80I49-64[»]
1A3EX-ray1.85I49-64[»]
1ABIX-ray2.30I54-64[»]
1C1UX-ray1.75I55-65[»]
1C1VX-ray1.98I55-65[»]
1C1WX-ray1.90I55-65[»]
1C4UX-ray2.10355-63[»]
1C5LX-ray1.47I55-64[»]
1C5NX-ray1.50I55-64[»]
1C5OX-ray1.90I55-65[»]
1D9IX-ray2.30I55-64[»]
1GHVX-ray1.85I55-65[»]
1GHWX-ray1.75I55-65[»]
1GHXX-ray1.65I55-65[»]
1GHYX-ray1.85I55-65[»]
1GJ4X-ray1.81I55-65[»]
1GJ5X-ray1.73I55-65[»]
1IHSX-ray2.00I49-65[»]
1NM6X-ray1.80B55-64[»]
1NT1X-ray2.00H55-64[»]
1NY2X-ray2.30355-64[»]
1O2GX-ray1.58I55-65[»]
1QBVX-ray1.80E55-65[»]
1SB1X-ray1.90I55-63[»]
1SL3X-ray1.81B55-64[»]
1TA2X-ray2.30B55-64[»]
1TA6X-ray1.90B55-64[»]
1TMTX-ray2.20J53-65[»]
1TWXX-ray2.40C55-64[»]
1VR1X-ray1.90I55-64[»]
1XM1X-ray2.30H55-64[»]
1YPEX-ray1.81I55-64[»]
1YPGX-ray1.80I55-64[»]
1YPJX-ray1.78I55-64[»]
1YPKX-ray1.78I55-64[»]
1YPLX-ray1.85I55-64[»]
1YPMX-ray1.85I55-64[»]
1ZRBX-ray1.90B55-65[»]
2GDEX-ray2.00D55-64[»]
2HGTX-ray2.20J54-64[»]
ProteinModelPortalP28504.
SMRP28504. Positions 1-65.
ModBaseSearch...

Protein family/group databases

Allergome9843. Hir me Hirudin.
MEROPSI14.001.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.70.10.10. 1 hit.
InterProIPR024793. Hirudin.
IPR000429. Prot_inh_hirudin.
IPR011061. Prot_inh_I14/15_hirudin/antisn.
[Graphical view]
PfamPF00713. Hirudin. 1 hit.
[Graphical view]
PIRSFPIRSF001640. Hirudin. 1 hit.
PRINTSPR00777. HIRUDIN.
ProDomPD004216. Prot_inh_hirudin. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF57262. Antihaemostatic. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP28504.

Entry information

Entry nameHIR2_HIRME
AccessionPrimary (citable) accession number: P28504
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: April 3, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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