P28496 (LAC1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sphingosine N-acyltransferase LAC1
EC=2.3.1.24

Gene names

Name:	LAC1
Synonyms:	DGT1
Ordered Locus Names:	YKL008C
ORF Names:	YKL156

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	418 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the ceramide synthase complex required for C26-CoA-dependent ceramide synthesis. Redundant with LAG1. Facilitates ER-to-Golgi transport of GPI-anchored proteins. Ref.5 Ref.6 Ref.7 Ref.12
Catalytic activity	Acyl-CoA + sphingosine = CoA + N-acylsphingosine.
Subunit structure	Composed of the ceramide synthase complex composed of at least LAC1, LAG1 and LIP1.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.5 Ref.9.
Induction	Expression is controlled by PDR1 and PDR3 which bind its propmoter PDRE element, and by ROX1 and CBF1. Ref.11
Miscellaneous	Present with 2840 molecules/cell in log phase SD medium. Ref.10
Sequence similarities	Belongs to the sphingosine N-acyltransferase family. Contains 1 TLC (TRAM/LAG1/CLN8) domain.

Ontologies

Keywords
Biological process	Lipid metabolism Lipid synthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ceramide biosynthetic process Inferred from direct assay Ref.12. Source: SGD
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	protein binding Inferred from physical interaction. Source: IntAct sphingosine N-acyltransferase activity Inferred from direct assay Ref.12. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
LAG1	P38703	6	EBI-26585,EBI-10035
LIP1	Q03579	6	EBI-26585,EBI-27640

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 418	418	Sphingosine N-acyltransferase LAC1		PRO_0000185535
Regions
	Topological domain	1 – 81	81	Cytoplasmic Potential
	Transmembrane	82 – 102	21	Helical; Potential
	Topological domain	103 – 134	32	Lumenal Potential
	Transmembrane	135 – 155	21	Helical; Potential
	Topological domain	156 – 172	17	Cytoplasmic Potential
	Transmembrane	173 – 193	21	Helical; Potential
	Topological domain	194 – 221	28	Lumenal Potential
	Transmembrane	222 – 242	21	Helical; Potential
	Topological domain	243 – 259	17	Cytoplasmic Potential
	Transmembrane	260 – 280	21	Helical; Potential
	Topological domain	281 – 296	16	Lumenal Potential
	Transmembrane	297 – 317	21	Helical; Potential
	Topological domain	318 – 355	38	Cytoplasmic Potential
	Transmembrane	356 – 376	21	Helical; Potential
	Topological domain	377 – 418	42	Lumenal Potential
	Domain	168 – 384	217	TLC
Amino acid modifications
	Glycosylation	103	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P28496 [UniParc]. Last modified June 1, 1994. Version 2. Checksum: 7691BA623AC0460A	FASTA	418	48,992
	10 20 30 40 50 60 MSTIKPSPSN NNLKVRSRPR RKSSIGKIDL GDTVPSLGTM FETKESKTAA KRRMQRLSEA 70 80 90 100 110 120 TKNDSDLVKK IWFSFREISY RHAWIAPLMI LIAVYSAYFT SGNTTKTNVL HRFVAVSYQI 130 140 150 160 170 180 GDTNAYGKGI NDLCFVFYYM IFFTFLREFL MDVVIRPFAI RLHVTSKHRI KRIMEQMYAI 190 200 210 220 230 240 FYTGVSGPFG IYCMYHSDLW FFNTKAMYRT YPDFTNPFLF KVFYLGQAAF WAQQACILVL 250 260 270 280 290 300 QLEKPRKDHN ELTFHHIVTL LLIWSSYVFH FTKMGLPIYI TMDVSDFLLS FSKTLNYLDS 310 320 330 340 350 360 GLAFFSFAIF VVAWIYLRHY INLKILWSVL TQFRTEGNYV LNFATQQYKC WISLPIVFVL 370 380 390 400 410 IGALQLVNLY WLFLIFRVLY RILWRGILKD DRSDSESDEE SDESSTTPTD STPTKKDI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of a 7.8 kb segment on the left arm of yeast chromosome XI reveals four open reading frames, including the CAP1 gene, an intron-containing gene and a gene encoding a homolog to the mammalian UOG-1 gene." Boyer J., Pascolo S., Richard G.-F., Dujon B. Yeast 9:279-287(1993) [PubMed: 8488728] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Effects of null mutations and overexpression of capping protein on morphogenesis, actin distribution and polarized secretion in yeast." Amatruda J.F., Gattermeir D.J., Karpova T.S., Cooper J.A. J. Cell Biol. 119:1151-1162(1992) [PubMed: 1447293] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-149.
[5]	"Two endoplasmic reticulum (ER) membrane proteins that facilitate ER-to-Golgi transport of glycosylphosphatidylinositol-anchored proteins." Barz W.P., Walter P. Mol. Biol. Cell 10:1043-1059(1999) [PubMed: 10198056] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]	"C26-CoA-dependent ceramide synthesis of Saccharomyces cerevisiae is operated by Lag1p and Lac1p." Guillas I., Kirchman P.A., Chuard R., Pfefferli M., Jiang J.C., Jazwinski S.M., Conzelmann A. EMBO J. 20:2655-2665(2001) [PubMed: 11387200] [Abstract] Cited for: FUNCTION.
[7]	"Lag1p and Lac1p are essential for the acyl-CoA-dependent ceramide synthase reaction in Saccharomyces cerevisae." Schorling S., Vallee B., Barz W.P., Riezman H., Oesterhelt D. Mol. Biol. Cell 12:3417-3427(2001) [PubMed: 11694577] [Abstract] Cited for: FUNCTION.
[8]	"TRAM, LAG1 and CLN8: members of a novel family of lipid-sensing domains?" Winter E., Ponting C.P. Trends Biochem. Sci. 27:381-383(2002) [PubMed: 12151215] [Abstract] Cited for: DOMAIN, TOPOLOGY.
[9]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[10]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]	"Differential regulation of ceramide synthase components LAC1 and LAG1 in Saccharomyces cerevisiae." Kolaczkowski M., Kolaczkowska A., Gaigg B., Schneiter R., Moye-Rowley W.S. Eukaryot. Cell 3:880-892(2004) [PubMed: 15302821] [Abstract] Cited for: INDUCTION.
[12]	"Lip1p: a novel subunit of acyl-CoA ceramide synthase." Vallee B., Riezman H. EMBO J. 24:730-741(2005) [PubMed: 15692566] [Abstract] Cited for: FUNCTION, MASS SPECTROMETRY, INTERACTION WITH LAG1 AND LIP1.
[13]	"Transmembrane topology of ceramide synthase in yeast." Kageyama-Yahara N., Riezman H. Biochem. J. 398:585-593(2006) [PubMed: 16756512] [Abstract] Cited for: TOPOLOGY.
[14]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X61398 Genomic DNA. Translation: CAA43670.1. S59773 Genomic DNA. Translation: AAC60549.1. Z28008 Genomic DNA. Translation: CAA81843.1. BK006944 Genomic DNA. Translation: DAA09148.1.
PIR	S30134.
RefSeq	NP_012917.1. NM_001179574.1.
3D structure databases
ProteinModelPortal	P28496.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4348N.
IntAct	P28496. 63 interactions.
MINT	MINT-395856.
STRING	P28496.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKL008C; YKL008C; YKL008C.
GeneID	853861.
KEGG	sce:YKL008C.
NMPDR	fig|4932.3.peg.3903.
Organism-specific databases
CYGD	YKL008c.
SGD	S000001491. LAC1.
Phylogenomic databases
eggNOG	fuNOG05004.
GeneTree	EFGT00050000005520.
HOGENOM	HBG738741.
OMA	MEQAYTA.
OrthoDB	EOG447K3B.
Gene expression databases
ArrayExpress	P28496.
Genevestigator	P28496.
GermOnline	YKL008C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR016439. Longevity_assurance_LAG1_LAC1. IPR006634. TLC-dom. IPR013599. TRAM1. [Graphical view]
KO	K04709.
Pfam	PF08390. TRAM1. 1 hit. PF03798. TRAM_LAG1_CLN8. 1 hit. [Graphical view]
PIRSF	PIRSF005225. LAG1_LAC1. 1 hit.
SMART	SM00724. TLC. 1 hit. [Graphical view]
PROSITE	PS50922. TLC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	975112.

Entry information

Entry name

LAC1_YEAST

Accession

Primary (citable) accession number: P28496
Secondary accession number(s): D6VXS8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	June 1, 1994
Last modified:	January 25, 2012
This is version 101 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program