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Protein

Sphingosine N-acyltransferase LAC1

Gene

LAC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ceramide synthase complex required for C26-CoA-dependent ceramide synthesis. Redundant with LAG1. Facilitates ER-to-Golgi transport of GPI-anchored proteins.4 Publications

Catalytic activityi

Acyl-CoA + sphingosine = CoA + N-acylsphingosine.

GO - Molecular functioni

  • sphingosine N-acyltransferase activity Source: SGD

GO - Biological processi

  • ceramide biosynthetic process Source: SGD
  • replicative cell aging Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER3O-373.
YEAST:MONOMER3O-373.

Chemistry

SwissLipidsiSLP:000000277.

Names & Taxonomyi

Protein namesi
Recommended name:
Sphingosine N-acyltransferase LAC1 (EC:2.3.1.24)
Gene namesi
Name:LAC1
Synonyms:DGT1
Ordered Locus Names:YKL008C
ORF Names:YKL156
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL008C.
SGDiS000001491. LAC1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 8180CytoplasmicSequence analysisAdd
BLAST
Transmembranei82 – 10221HelicalSequence analysisAdd
BLAST
Topological domaini103 – 13432LumenalSequence analysisAdd
BLAST
Transmembranei135 – 15521HelicalSequence analysisAdd
BLAST
Topological domaini156 – 17217CytoplasmicSequence analysisAdd
BLAST
Transmembranei173 – 19321HelicalSequence analysisAdd
BLAST
Topological domaini194 – 22128LumenalSequence analysisAdd
BLAST
Transmembranei222 – 24221HelicalSequence analysisAdd
BLAST
Topological domaini243 – 25917CytoplasmicSequence analysisAdd
BLAST
Transmembranei260 – 28021HelicalSequence analysisAdd
BLAST
Topological domaini281 – 29616LumenalSequence analysisAdd
BLAST
Transmembranei297 – 31721HelicalSequence analysisAdd
BLAST
Topological domaini318 – 35538CytoplasmicSequence analysisAdd
BLAST
Transmembranei356 – 37621HelicalSequence analysisAdd
BLAST
Topological domaini377 – 41842LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

  • acyl-CoA ceramide synthase complex Source: SGD
  • endoplasmic reticulum Source: SGD
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 418417Sphingosine N-acyltransferase LAC1PRO_0000185535Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineCombined sources
Modified residuei23 – 231PhosphoserineBy similarity
Modified residuei24 – 241PhosphoserineBy similarity
Glycosylationi103 – 1031N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP28496.

PTM databases

iPTMnetiP28496.

Expressioni

Inductioni

Expression is controlled by PDR1 and PDR3 which bind its propmoter PDRE element, and by ROX1 and CBF1.1 Publication

Interactioni

Subunit structurei

Composed of the ceramide synthase complex composed of at least LAC1, LAG1 and LIP1.

Binary interactionsi

WithEntry#Exp.IntActNotes
LAG1P387037EBI-26585,EBI-10035
LIP1Q035796EBI-26585,EBI-27640

Protein-protein interaction databases

BioGridi34124. 67 interactions.
DIPiDIP-4348N.
IntActiP28496. 58 interactions.
MINTiMINT-395856.

Structurei

3D structure databases

ProteinModelPortaliP28496.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini168 – 384217TLCPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 TLC (TRAM/LAG1/CLN8) domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00730000113269.
HOGENOMiHOG000216359.
InParanoidiP28496.
KOiK04709.
OMAiGANYMEL.
OrthoDBiEOG092C45Y7.

Family and domain databases

InterProiIPR016439. Lag1/Lac1.
IPR006634. TLC-dom.
IPR013599. TRAM1.
[Graphical view]
PANTHERiPTHR12560. PTHR12560. 1 hit.
PfamiPF08390. TRAM1. 1 hit.
PF03798. TRAM_LAG1_CLN8. 1 hit.
[Graphical view]
PIRSFiPIRSF005225. LAG1_LAC1. 1 hit.
SMARTiSM00724. TLC. 1 hit.
[Graphical view]
PROSITEiPS50922. TLC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28496-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTIKPSPSN NNLKVRSRPR RKSSIGKIDL GDTVPSLGTM FETKESKTAA
60 70 80 90 100
KRRMQRLSEA TKNDSDLVKK IWFSFREISY RHAWIAPLMI LIAVYSAYFT
110 120 130 140 150
SGNTTKTNVL HRFVAVSYQI GDTNAYGKGI NDLCFVFYYM IFFTFLREFL
160 170 180 190 200
MDVVIRPFAI RLHVTSKHRI KRIMEQMYAI FYTGVSGPFG IYCMYHSDLW
210 220 230 240 250
FFNTKAMYRT YPDFTNPFLF KVFYLGQAAF WAQQACILVL QLEKPRKDHN
260 270 280 290 300
ELTFHHIVTL LLIWSSYVFH FTKMGLPIYI TMDVSDFLLS FSKTLNYLDS
310 320 330 340 350
GLAFFSFAIF VVAWIYLRHY INLKILWSVL TQFRTEGNYV LNFATQQYKC
360 370 380 390 400
WISLPIVFVL IGALQLVNLY WLFLIFRVLY RILWRGILKD DRSDSESDEE
410
SDESSTTPTD STPTKKDI
Length:418
Mass (Da):48,992
Last modified:June 1, 1994 - v2
Checksum:i7691BA623AC0460A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61398 Genomic DNA. Translation: CAA43670.1.
S59773 Genomic DNA. Translation: AAC60549.1.
Z28008 Genomic DNA. Translation: CAA81843.1.
BK006944 Genomic DNA. Translation: DAA09148.1.
PIRiS30134.
RefSeqiNP_012917.3. NM_001179574.3.

Genome annotation databases

EnsemblFungiiYKL008C; YKL008C; YKL008C.
GeneIDi853861.
KEGGisce:YKL008C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61398 Genomic DNA. Translation: CAA43670.1.
S59773 Genomic DNA. Translation: AAC60549.1.
Z28008 Genomic DNA. Translation: CAA81843.1.
BK006944 Genomic DNA. Translation: DAA09148.1.
PIRiS30134.
RefSeqiNP_012917.3. NM_001179574.3.

3D structure databases

ProteinModelPortaliP28496.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34124. 67 interactions.
DIPiDIP-4348N.
IntActiP28496. 58 interactions.
MINTiMINT-395856.

Chemistry

SwissLipidsiSLP:000000277.

PTM databases

iPTMnetiP28496.

Proteomic databases

MaxQBiP28496.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL008C; YKL008C; YKL008C.
GeneIDi853861.
KEGGisce:YKL008C.

Organism-specific databases

EuPathDBiFungiDB:YKL008C.
SGDiS000001491. LAC1.

Phylogenomic databases

GeneTreeiENSGT00730000113269.
HOGENOMiHOG000216359.
InParanoidiP28496.
KOiK04709.
OMAiGANYMEL.
OrthoDBiEOG092C45Y7.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER3O-373.
YEAST:MONOMER3O-373.

Miscellaneous databases

PROiP28496.

Family and domain databases

InterProiIPR016439. Lag1/Lac1.
IPR006634. TLC-dom.
IPR013599. TRAM1.
[Graphical view]
PANTHERiPTHR12560. PTHR12560. 1 hit.
PfamiPF08390. TRAM1. 1 hit.
PF03798. TRAM_LAG1_CLN8. 1 hit.
[Graphical view]
PIRSFiPIRSF005225. LAG1_LAC1. 1 hit.
SMARTiSM00724. TLC. 1 hit.
[Graphical view]
PROSITEiPS50922. TLC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLAC1_YEAST
AccessioniPrimary (citable) accession number: P28496
Secondary accession number(s): D6VXS8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: June 1, 1994
Last modified: September 7, 2016
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2840 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.