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Protein

Sphingosine N-acyltransferase LAC1

Gene

LAC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the ceramide synthase complex required for C26-CoA-dependent ceramide synthesis. Redundant with LAG1. Facilitates ER-to-Golgi transport of GPI-anchored proteins.4 Publications

Miscellaneous

Present with 2840 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Acyl-CoA + sphingosine = CoA + N-acylsphingosine.

GO - Molecular functioni

  • sphingosine N-acyltransferase activity Source: SGD

GO - Biological processi

  • ceramide biosynthetic process Source: SGD
  • replicative cell aging Source: SGD

Keywordsi

Molecular functionTransferase
Biological processLipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER3O-373
YEAST:MONOMER3O-373

Chemistry databases

SwissLipidsiSLP:000000277

Names & Taxonomyi

Protein namesi
Recommended name:
Sphingosine N-acyltransferase LAC1 (EC:2.3.1.24)
Gene namesi
Name:LAC1
Synonyms:DGT1
Ordered Locus Names:YKL008C
ORF Names:YKL156
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL008C
SGDiS000001491 LAC1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 81CytoplasmicSequence analysisAdd BLAST80
Transmembranei82 – 102HelicalSequence analysisAdd BLAST21
Topological domaini103 – 134LumenalSequence analysisAdd BLAST32
Transmembranei135 – 155HelicalSequence analysisAdd BLAST21
Topological domaini156 – 172CytoplasmicSequence analysisAdd BLAST17
Transmembranei173 – 193HelicalSequence analysisAdd BLAST21
Topological domaini194 – 221LumenalSequence analysisAdd BLAST28
Transmembranei222 – 242HelicalSequence analysisAdd BLAST21
Topological domaini243 – 259CytoplasmicSequence analysisAdd BLAST17
Transmembranei260 – 280HelicalSequence analysisAdd BLAST21
Topological domaini281 – 296LumenalSequence analysisAdd BLAST16
Transmembranei297 – 317HelicalSequence analysisAdd BLAST21
Topological domaini318 – 355CytoplasmicSequence analysisAdd BLAST38
Transmembranei356 – 376HelicalSequence analysisAdd BLAST21
Topological domaini377 – 418LumenalSequence analysisAdd BLAST42

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001855352 – 418Sphingosine N-acyltransferase LAC1Add BLAST417

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei23PhosphoserineBy similarity1
Modified residuei24PhosphoserineBy similarity1
Glycosylationi103N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP28496
PaxDbiP28496
PRIDEiP28496

PTM databases

iPTMnetiP28496

Expressioni

Inductioni

Expression is controlled by PDR1 and PDR3 which bind its propmoter PDRE element, and by ROX1 and CBF1.1 Publication

Interactioni

Subunit structurei

Composed of the ceramide synthase complex composed of at least LAC1, LAG1 and LIP1.

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi34124, 89 interactors
DIPiDIP-4348N
IntActiP28496, 58 interactors
MINTiP28496
STRINGi4932.YKL008C

Structurei

3D structure databases

ProteinModelPortaliP28496
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini168 – 384TLCPROSITE-ProRule annotationAdd BLAST217

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00730000113269
HOGENOMiHOG000216359
InParanoidiP28496
KOiK04709
OMAiDFAAIPF
OrthoDBiEOG092C45Y7

Family and domain databases

InterProiView protein in InterPro
IPR016439 Lag1/Lac1-like
IPR006634 TLC-dom
IPR013599 TRAM1
PANTHERiPTHR12560 PTHR12560, 1 hit
PfamiView protein in Pfam
PF08390 TRAM1, 1 hit
PF03798 TRAM_LAG1_CLN8, 1 hit
PIRSFiPIRSF005225 LAG1_LAC1, 1 hit
SMARTiView protein in SMART
SM00724 TLC, 1 hit
PROSITEiView protein in PROSITE
PS50922 TLC, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28496-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTIKPSPSN NNLKVRSRPR RKSSIGKIDL GDTVPSLGTM FETKESKTAA
60 70 80 90 100
KRRMQRLSEA TKNDSDLVKK IWFSFREISY RHAWIAPLMI LIAVYSAYFT
110 120 130 140 150
SGNTTKTNVL HRFVAVSYQI GDTNAYGKGI NDLCFVFYYM IFFTFLREFL
160 170 180 190 200
MDVVIRPFAI RLHVTSKHRI KRIMEQMYAI FYTGVSGPFG IYCMYHSDLW
210 220 230 240 250
FFNTKAMYRT YPDFTNPFLF KVFYLGQAAF WAQQACILVL QLEKPRKDHN
260 270 280 290 300
ELTFHHIVTL LLIWSSYVFH FTKMGLPIYI TMDVSDFLLS FSKTLNYLDS
310 320 330 340 350
GLAFFSFAIF VVAWIYLRHY INLKILWSVL TQFRTEGNYV LNFATQQYKC
360 370 380 390 400
WISLPIVFVL IGALQLVNLY WLFLIFRVLY RILWRGILKD DRSDSESDEE
410
SDESSTTPTD STPTKKDI
Length:418
Mass (Da):48,992
Last modified:June 1, 1994 - v2
Checksum:i7691BA623AC0460A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61398 Genomic DNA Translation: CAA43670.1
S59773 Genomic DNA Translation: AAC60549.1
Z28008 Genomic DNA Translation: CAA81843.1
BK006944 Genomic DNA Translation: DAA09148.1
PIRiS30134
RefSeqiNP_012917.3, NM_001179574.3

Genome annotation databases

EnsemblFungiiYKL008C; YKL008C; YKL008C
GeneIDi853861
KEGGisce:YKL008C

Similar proteinsi

Entry informationi

Entry nameiLAC1_YEAST
AccessioniPrimary (citable) accession number: P28496
Secondary accession number(s): D6VXS8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: June 1, 1994
Last modified: May 23, 2018
This is version 157 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

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