F-actin-capping protein subunit alpha - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P28495 (CAPZA_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 116. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
F-actin-capping protein subunit alpha

Gene names

Name:	CAP1
Ordered Locus Names:	YKL007W
ORF Names:	YKL155

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	268 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	F-actin-capping proteins bind in a Ca²⁺-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.
Subunit structure	Heterodimer of an alpha and a beta subunit.
Miscellaneous	Present with 4380 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the F-actin-capping protein alpha subunit family.

Ontologies

Keywords
Ligand	Actin-binding
Molecular function	Actin capping
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	actin cytoskeleton organization Inferred from electronic annotation. Source: InterPro barbed-end actin filament capping Inferred from direct assay PubMed 14769858. Source: SGD
Cellular_component	F-actin capping protein complex Inferred from physical interaction PubMed 14769858. Source: SGD actin cortical patch Inferred from direct assay PubMed 14769858. Source: SGD actin filament Inferred from physical interaction PubMed 14769858. Source: SGD cellular bud neck Inferred from direct assay PubMed 22842922. Source: SGD cellular bud tip Inferred from direct assay PubMed 22842922. Source: SGD mating projection tip Inferred from direct assay PubMed 19053807. Source: SGD plasma membrane Inferred from direct assay PubMed 22842922. Source: SGD
Molecular_function	actin filament binding Inferred from genetic interaction PubMed 14769858. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CAP2	P13517	5	EBI-4003,EBI-4013

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 268

268

F-actin-capping protein subunit alpha

PRO_0000208646

Amino acid modifications

Modified residue

Phosphoserine Ref.7

Sequences

Sequence

Length

Mass (Da)

Tools

P28495 [UniParc].

Last modified December 1, 1992. Version 1.
Checksum: 6FBD3130B55B0C5C
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FASTA

268

30,699

        10         20         30         40         50         60 
MSSSKFEEVI NKIINDSPPG ELREVYDDLI KITSENSKNT ILDAIENYNV QNCIPIEVNG 

        70         80         90        100        110        120 
NSVIISKYNK EGAKFFDPVN SVIFSVNHLE RKGLDIEPYE FTHAKIEKGQ LKELHDKLHE 

       130        140        150        160        170        180 
YLLQSFPGDV SFAVYPVPEE ISKISIIIVS TKYNPNNFWN GHWRSSYIYD LETRELSGQI 

       190        200        210        220        230        240 
STQVHYYEDG NVSFQSGKDI NQSNVDDVVC TIRDIETNFE NDLDLSFFDL NEKQFKALRR 

       250        260 
RLPVTRSKIN WGSAIGSYRL GKNAAEGK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Effects of null mutations and overexpression of capping protein on morphogenesis, actin distribution and polarized secretion in yeast." Amatruda J.F., Gattermeir D.J., Karpova T.S., Cooper J.A. J. Cell Biol. 119:1151-1162(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]	"Sequence of a 7.8 kb segment on the left arm of yeast chromosome XI reveals four open reading frames, including the CAP1 gene, an intron-containing gene and a gene encoding a homolog to the mammalian UOG-1 gene." Boyer J., Pascolo S., Richard G.-F., Dujon B. Yeast 9:279-287(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. Mewes H.-W. Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S59773 Genomic DNA. Translation: AAC60553.1. X61398 Genomic DNA. Translation: CAA43669.1. Z28007 Genomic DNA. Translation: CAA81841.1. AY558093 Genomic DNA. Translation: AAS56419.1. BK006944 Genomic DNA. Translation: DAA09149.1.
PIR	S30135.
RefSeq	NP_012918.1. NM_001179573.1.
3D structure databases
ProteinModelPortal	P28495.
SMR	P28495. Positions 12-262.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2696N.
IntAct	P28495. 13 interactions.
MINT	MINT-500609.
STRING	4932.YKL007W.
Proteomic databases
PaxDb	P28495.
PeptideAtlas	P28495.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKL007W; YKL007W; YKL007W.
GeneID	853862.
KEGG	sce:YKL007W.
Organism-specific databases
CYGD	YKL007w.
SGD	S000001490. CAP1.
Phylogenomic databases
eggNOG	NOG261759.
GeneTree	ENSGT00390000006476.
HOGENOM	HOG000036539.
KO	K10364.
OMA	TRHNELE.
OrthoDB	EOG45XC5F.
Gene expression databases
Genevestigator	P28495.
GermOnline	YKL007W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR017865. F-actin_cap_asu_CS. IPR002189. WASH_F-actin_cap_alpha. [Graphical view]
PANTHER	PTHR10653. PTHR10653. 1 hit.
Pfam	PF01267. F-actin_cap_A. 1 hit. [Graphical view]
PRINTS	PR00191. FACTINCAPA.
PROSITE	PS00748. F_ACTIN_CAPPING_A_1. 1 hit. PS00749. F_ACTIN_CAPPING_A_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	975115.

Entry information

Entry name

CAPZA_YEAST

Accession

Primary (citable) accession number: P28495
Secondary accession number(s): D6VXS9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	May 1, 2013
This is version 116 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents