SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P28494

- MA2A1_RAT

UniProt

P28494 - MA2A1_RAT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Alpha-mannosidase 2
Gene
Man2a1, Mana2
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway.

Catalytic activityi

Hydrolysis of the terminal (1->3)- and (1->6)-linked alpha-D-mannose residues in the mannosyl-oligosaccharide Man5(GlcNAc)3.

Cofactori

Binds 1 zinc ion per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi141 – 1411Zinc By similarity
Metal bindingi143 – 1431Zinc By similarity
Active sitei255 – 2551Nucleophile By similarity
Metal bindingi255 – 2551Zinc By similarity

GO - Molecular functioni

  1. mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase activity Source: UniProtKB-EC
  2. zinc ion binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGH38. Glycoside Hydrolase Family 38.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-mannosidase 2 (EC:3.2.1.114)
Alternative name(s):
Golgi alpha-mannosidase II
Short name:
AMan II
Short name:
Man II
Mannosidase alpha class 2A member 1
Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase
Gene namesi
Name:Man2a1
Synonyms:Mana2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi3038. Man2a1.

Subcellular locationi

GO - Cellular componenti

  1. Golgi membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – 489›489Alpha-mannosidase 2
PRO_0000206904Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi45 – 451N-linked (GlcNAc...) Reviewed prediction
Modified residuei47 – 471Phosphoserine By similarity
Modified residuei49 – 491Phosphoserine By similarity
Glycosylationi60 – 601N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

Glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP28494.
PRIDEiP28494.

PTM databases

PhosphoSiteiP28494.

Expressioni

Tissue specificityi

All tissues, mostly in adrenal and thymus.

Gene expression databases

GenevestigatoriP28494.

Interactioni

Subunit structurei

Homodimer; disulfide-linked.2 Publications

Protein-protein interaction databases

IntActiP28494. 1 interaction.
MINTiMINT-4574652.
STRINGi10116.ENSRNOP00000020767.

Structurei

3D structure databases

ProteinModelPortaliP28494.
SMRiP28494. Positions 82-488.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0383.
HOGENOMiHOG000293253.
HOVERGENiHBG052390.
InParanoidiP28494.

Family and domain databases

Gene3Di1.20.1270.50. 1 hit.
3.20.110.10. 1 hit.
InterProiIPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR027291. Glyco_hydro_38/57_N.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view]
PfamiPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
[Graphical view]
SUPFAMiSSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.

Sequencei

Sequence statusi: Fragment.

P28494-1 [UniParc]FASTAAdd to Basket

« Hide

GFSPHIIRVE RKGQLSILQE KIDHLERLLA ENNEIISNIR DSVINLSESV    50
EDGPRGPAGN ASQGSAHLHS AQLALQADPK DCLFASQSGN QHRDVQMLDV 100
YDLIPFDNPD GGVWKQGFDI KYEADEWDRE PLQVFVVPHS HNDPGWLKTF 150
NDYFRDKTQY IFNNMVLKLK EDSSRKFIWS EISYLAKWWD IIDNPKKEAV 200
KSLLQNGQLE IVTGGWVMAD EATTHYFALI DQLIEGHQWL EKNLGVKPRS 250
GWAIDPFGHS PTMTYLLKRA GFSHMLIQRV HYSVKKHFSL QKTLEFFWRQ 300
NWDLGSTTDI LCHMMPFYSY DIPHTCGPDP KICCQFDFKR LPGGRYGCPW 350
GVPPEAISPG NVQSRAQMLL DQYRKKSKLF RTKVLLAPLG DDFRFSEYTE 400
WDLQYRNYEQ LFSYMNSQPH LKVKIQFGTL SDYFDALEKS VAAEKKGGQS 450
VFPALSGDFF TYADRDDHYW SGYFTSRPFY KRMDRIMEF 489
Length:489
Mass (Da):56,736
Last modified:December 1, 1992 - v1
Checksum:i8193E9CDAD3AFAF5
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti124 – 1241A → G AA sequence 1 Publication
Sequence conflicti127 – 1271W → I AA sequence 1 Publication
Sequence conflicti211 – 2111I → G AA sequence 1 Publication
Sequence conflicti214 – 2141G → K AA sequence 1 Publication
Sequence conflicti460 – 4601F → I AA sequence 1 Publication

Non-terminal residue

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M24353 mRNA. Translation: AAA66457.1. Different termination.
PIRiA33901.
UniGeneiRn.163804.

Genome annotation databases

UCSCiRGD:3038. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M24353 mRNA. Translation: AAA66457.1 . Different termination.
PIRi A33901.
UniGenei Rn.163804.

3D structure databases

ProteinModelPortali P28494.
SMRi P28494. Positions 82-488.
ModBasei Search...

Protein-protein interaction databases

IntActi P28494. 1 interaction.
MINTi MINT-4574652.
STRINGi 10116.ENSRNOP00000020767.

Chemistry

ChEMBLi CHEMBL2257.

Protein family/group databases

CAZyi GH38. Glycoside Hydrolase Family 38.

PTM databases

PhosphoSitei P28494.

Proteomic databases

PaxDbi P28494.
PRIDEi P28494.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:3038. rat.

Organism-specific databases

RGDi 3038. Man2a1.

Phylogenomic databases

eggNOGi COG0383.
HOGENOMi HOG000293253.
HOVERGENi HBG052390.
InParanoidi P28494.

Enzyme and pathway databases

UniPathwayi UPA00378 .

Miscellaneous databases

PROi P28494.

Gene expression databases

Genevestigatori P28494.

Family and domain databases

Gene3Di 1.20.1270.50. 1 hit.
3.20.110.10. 1 hit.
InterProi IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR027291. Glyco_hydro_38/57_N.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view ]
Pfami PF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
[Graphical view ]
SUPFAMi SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Isolation of a rat liver Golgi mannosidase II clone by mixed oligonucleotide-primed amplification of cDNA."
    Moremen K.W.
    Proc. Natl. Acad. Sci. U.S.A. 86:5276-5280(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Liver.
  2. "Mannosidase II and the 135-kDa Golgi-specific antigen recognized monoclonal antibody 53FC3 are the same dimeric protein."
    Baron M.D., Garoff H.
    J. Biol. Chem. 265:19928-19931(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 123-138 AND 210-223, SUBUNIT.
  3. "Novel purification of the catalytic domain of Golgi alpha-mannosidase II. Characterization and comparison with the intact enzyme."
    Moremen K.W., Touster O., Robbins P.W.
    J. Biol. Chem. 266:16876-16885(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 74-103 AND 445-463, GLYCOSYLATION, SUBUNIT.
    Tissue: Liver.

Entry informationi

Entry nameiMA2A1_RAT
AccessioniPrimary (citable) accession number: P28494
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: June 11, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi