Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P28494

- MA2A1_RAT

UniProt

P28494 - MA2A1_RAT

Protein

Alpha-mannosidase 2

Gene

Man2a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 1 (01 Dec 1992)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway.

    Catalytic activityi

    Hydrolysis of the terminal (1->3)- and (1->6)-linked alpha-D-mannose residues in the mannosyl-oligosaccharide Man5(GlcNAc)3.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi141 – 1411ZincBy similarity
    Metal bindingi143 – 1431ZincBy similarity
    Active sitei255 – 2551NucleophileBy similarity
    Metal bindingi255 – 2551ZincBy similarity

    GO - Molecular functioni

    1. mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase activity Source: UniProtKB-EC
    2. zinc ion binding Source: InterPro

    GO - Biological processi

    1. protein glycosylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGH38. Glycoside Hydrolase Family 38.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-mannosidase 2 (EC:3.2.1.114)
    Alternative name(s):
    Golgi alpha-mannosidase II
    Short name:
    AMan II
    Short name:
    Man II
    Mannosidase alpha class 2A member 1
    Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase
    Gene namesi
    Name:Man2a1
    Synonyms:Mana2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi3038. Man2a1.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini‹1 – 489›489Alpha-mannosidase 2PRO_0000206904Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi45 – 451N-linked (GlcNAc...)Sequence Analysis
    Modified residuei47 – 471PhosphoserineBy similarity
    Modified residuei49 – 491PhosphoserineBy similarity
    Glycosylationi60 – 601N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Glycosylated.1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiP28494.
    PRIDEiP28494.

    PTM databases

    PhosphoSiteiP28494.

    Expressioni

    Tissue specificityi

    All tissues, mostly in adrenal and thymus.

    Gene expression databases

    GenevestigatoriP28494.

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked.2 Publications

    Protein-protein interaction databases

    IntActiP28494. 1 interaction.
    MINTiMINT-4574652.
    STRINGi10116.ENSRNOP00000020767.

    Structurei

    3D structure databases

    ProteinModelPortaliP28494.
    SMRiP28494. Positions 82-488.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 38 family.Curated

    Phylogenomic databases

    eggNOGiCOG0383.
    HOGENOMiHOG000293253.
    HOVERGENiHBG052390.
    InParanoidiP28494.

    Family and domain databases

    Gene3Di1.20.1270.50. 1 hit.
    3.20.110.10. 1 hit.
    InterProiIPR011330. Glyco_hydro/deAcase_b/a-brl.
    IPR027291. Glyco_hydro_38/57_N.
    IPR015341. Glyco_hydro_38_cen.
    IPR000602. Glyco_hydro_38_N.
    IPR028995. Glyco_hydro_57/38_cen.
    [Graphical view]
    PfamiPF09261. Alpha-mann_mid. 1 hit.
    PF01074. Glyco_hydro_38. 1 hit.
    [Graphical view]
    SUPFAMiSSF88688. SSF88688. 1 hit.
    SSF88713. SSF88713. 1 hit.

    Sequencei

    Sequence statusi: Fragment.

    P28494-1 [UniParc]FASTAAdd to Basket

    « Hide

    GFSPHIIRVE RKGQLSILQE KIDHLERLLA ENNEIISNIR DSVINLSESV    50
    EDGPRGPAGN ASQGSAHLHS AQLALQADPK DCLFASQSGN QHRDVQMLDV 100
    YDLIPFDNPD GGVWKQGFDI KYEADEWDRE PLQVFVVPHS HNDPGWLKTF 150
    NDYFRDKTQY IFNNMVLKLK EDSSRKFIWS EISYLAKWWD IIDNPKKEAV 200
    KSLLQNGQLE IVTGGWVMAD EATTHYFALI DQLIEGHQWL EKNLGVKPRS 250
    GWAIDPFGHS PTMTYLLKRA GFSHMLIQRV HYSVKKHFSL QKTLEFFWRQ 300
    NWDLGSTTDI LCHMMPFYSY DIPHTCGPDP KICCQFDFKR LPGGRYGCPW 350
    GVPPEAISPG NVQSRAQMLL DQYRKKSKLF RTKVLLAPLG DDFRFSEYTE 400
    WDLQYRNYEQ LFSYMNSQPH LKVKIQFGTL SDYFDALEKS VAAEKKGGQS 450
    VFPALSGDFF TYADRDDHYW SGYFTSRPFY KRMDRIMEF 489
    Length:489
    Mass (Da):56,736
    Last modified:December 1, 1992 - v1
    Checksum:i8193E9CDAD3AFAF5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11
    Sequence conflicti124 – 1241A → G AA sequence (PubMed:2246269)Curated
    Sequence conflicti127 – 1271W → I AA sequence (PubMed:2246269)Curated
    Sequence conflicti211 – 2111I → G AA sequence (PubMed:2246269)Curated
    Sequence conflicti214 – 2141G → K AA sequence (PubMed:2246269)Curated
    Sequence conflicti460 – 4601F → I AA sequence (PubMed:1885615)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M24353 mRNA. Translation: AAA66457.1. Different termination.
    PIRiA33901.
    UniGeneiRn.163804.

    Genome annotation databases

    UCSCiRGD:3038. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M24353 mRNA. Translation: AAA66457.1 . Different termination.
    PIRi A33901.
    UniGenei Rn.163804.

    3D structure databases

    ProteinModelPortali P28494.
    SMRi P28494. Positions 82-488.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P28494. 1 interaction.
    MINTi MINT-4574652.
    STRINGi 10116.ENSRNOP00000020767.

    Chemistry

    ChEMBLi CHEMBL2257.

    Protein family/group databases

    CAZyi GH38. Glycoside Hydrolase Family 38.

    PTM databases

    PhosphoSitei P28494.

    Proteomic databases

    PaxDbi P28494.
    PRIDEi P28494.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi RGD:3038. rat.

    Organism-specific databases

    RGDi 3038. Man2a1.

    Phylogenomic databases

    eggNOGi COG0383.
    HOGENOMi HOG000293253.
    HOVERGENi HBG052390.
    InParanoidi P28494.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .

    Miscellaneous databases

    PROi P28494.

    Gene expression databases

    Genevestigatori P28494.

    Family and domain databases

    Gene3Di 1.20.1270.50. 1 hit.
    3.20.110.10. 1 hit.
    InterProi IPR011330. Glyco_hydro/deAcase_b/a-brl.
    IPR027291. Glyco_hydro_38/57_N.
    IPR015341. Glyco_hydro_38_cen.
    IPR000602. Glyco_hydro_38_N.
    IPR028995. Glyco_hydro_57/38_cen.
    [Graphical view ]
    Pfami PF09261. Alpha-mann_mid. 1 hit.
    PF01074. Glyco_hydro_38. 1 hit.
    [Graphical view ]
    SUPFAMi SSF88688. SSF88688. 1 hit.
    SSF88713. SSF88713. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Isolation of a rat liver Golgi mannosidase II clone by mixed oligonucleotide-primed amplification of cDNA."
      Moremen K.W.
      Proc. Natl. Acad. Sci. U.S.A. 86:5276-5280(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Liver.
    2. "Mannosidase II and the 135-kDa Golgi-specific antigen recognized monoclonal antibody 53FC3 are the same dimeric protein."
      Baron M.D., Garoff H.
      J. Biol. Chem. 265:19928-19931(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 123-138 AND 210-223, SUBUNIT.
    3. "Novel purification of the catalytic domain of Golgi alpha-mannosidase II. Characterization and comparison with the intact enzyme."
      Moremen K.W., Touster O., Robbins P.W.
      J. Biol. Chem. 266:16876-16885(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 74-103 AND 445-463, GLYCOSYLATION, SUBUNIT.
      Tissue: Liver.

    Entry informationi

    Entry nameiMA2A1_RAT
    AccessioniPrimary (citable) accession number: P28494
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 122 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3