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Protein

Glutaminase liver isoform, mitochondrial

Gene

Gls2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production (By similarity).By similarity

Catalytic activityi

L-glutamine + H2O = L-glutamate + NH3.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei219 – 2191SubstrateBy similarity
Binding sitei268 – 2681SubstrateBy similarity
Binding sitei314 – 3141SubstrateBy similarity
Binding sitei321 – 3211SubstrateBy similarity
Binding sitei347 – 3471SubstrateBy similarity
Binding sitei399 – 3991SubstrateBy similarity
Binding sitei417 – 4171Substrate; via amide nitrogenBy similarity

GO - Molecular functioni

  1. glutaminase activity Source: UniProtKB-EC

GO - Biological processi

  1. glutamine metabolic process Source: InterPro
  2. reactive oxygen species metabolic process Source: Ensembl
  3. regulation of apoptotic process Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13074.
ReactomeiREACT_214662. Amino acid synthesis and interconversion (transamination).
REACT_227883. Glutamate Neurotransmitter Release Cycle.
SABIO-RKP28492.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutaminase liver isoform, mitochondrial (EC:3.5.1.2)
Short name:
GLS
Alternative name(s):
L-glutaminase
L-glutamine amidohydrolase
Gene namesi
Name:Gls2
Synonyms:Ga
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 7

Organism-specific databases

RGDi620359. Gls2.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial matrix Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 1414MitochondrionSequence AnalysisAdd
BLAST
Chaini15 – 602588Glutaminase liver isoform, mitochondrialPRO_0000011627Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei253 – 2531N6-succinyllysineBy similarity
Modified residuei279 – 2791N6-acetyllysineBy similarity
Modified residuei284 – 2841N6-acetyllysineBy similarity
Modified residuei329 – 3291N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP28492.
PRIDEiP28492.

Expressioni

Tissue specificityi

Liver specific.

Gene expression databases

GenevestigatoriP28492.

Interactioni

Subunit structurei

Interacts with the PDZ domain of the syntrophin SNTA1. Interacts with the PDZ domain of TAX1BP3 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP28492.
SMRiP28492. Positions 155-464.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati518 – 55134ANK 1Add
BLAST
Repeati552 – 58534ANK 2Add
BLAST

Sequence similaritiesi

Belongs to the glutaminase family.Curated
Contains 2 ANK repeats.Curated

Keywords - Domaini

ANK repeat, Repeat, Transit peptide

Phylogenomic databases

eggNOGiCOG2066.
GeneTreeiENSGT00390000010463.
HOGENOMiHOG000216891.
HOVERGENiHBG005856.
InParanoidiP28492.
KOiK01425.
OMAiCVKPLTY.
OrthoDBiEOG7S4X5F.
PhylomeDBiP28492.
TreeFamiTF313359.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
3.40.710.10. 1 hit.
HAMAPiMF_00313. Glutaminase.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR012338. Beta-lactam/transpept-like.
IPR015868. Glutaminase.
[Graphical view]
PANTHERiPTHR12544. PTHR12544. 1 hit.
PfamiPF12796. Ank_2. 1 hit.
PF04960. Glutaminase. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF56601. SSF56601. 1 hit.
TIGRFAMsiTIGR03814. Gln_ase. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P28492-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRSMRALQNA LSRAGSHGQR GGWGHPSRGP LLGGGVRYYF GEAAAQGRGT
60 70 80 90 100
PHSHQPQHSD HDASNSGMLP RLGDLLFYTI AEGQERIPIH KFTTALKATG
110 120 130 140 150
LQTSDPRLQD CMSKMQRMVQ ESSSGGLLDR ELFQKCVSSN IVLLTQAFRK
160 170 180 190 200
KFVIPDFEEF TGHVDRIFED AKELTGGKVA AYIPHLAKSN PDLWGVSLCT
210 220 230 240 250
VDGQRHSVGH TKIPFCLQSC VKPLTYAISV STLGTDYVHK FVGKEPSGLR
260 270 280 290 300
YNKLSLNEEG IPHNPMVNAG AIVVSSLIKM DCNKAEKFDF VLQYLNKMAG
310 320 330 340 350
NEFMGFSNAT FQSEKETGDR NYAIGYYLKE KKCFPKGVDM MAALDLYFQL
360 370 380 390 400
CSVEVTCESG SVMAATLANG GICPITGESV LSAEAVRNTL SLMHSCGMYD
410 420 430 440 450
FSGQFAFHVG LPAKSAVSGA ILLVVPNVMG MMCLSPPLDK LGNSHRGISF
460 470 480 490 500
CQKLVSLFNF HNYDNLRHCA RKLDPRREGG EVRNKTVVNL LFAAYSGDVS
510 520 530 540 550
ALRRFALSAV DMEQKDYDSR TALHVAAAEG HIDVVKFLIE ACKVNPFVKD
560 570 580 590 600
RWGNIPLDDA VQFNHLEVVK LLQDYHDSYM LSETQAEVAA ETLSKENLES

MV
Length:602
Mass (Da):66,248
Last modified:August 30, 2005 - v3
Checksum:iF4AE3BD30626A00F
GO
Isoform 2 (identifier: P28492-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-67: Missing.

Show »
Length:535
Mass (Da):59,204
Checksum:iE608D53D78B3F7F1
GO

Sequence cautioni

The sequence AAH89776.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti76 – 761L → V in AAC37708. (PubMed:9164856)Curated
Sequence conflicti336 – 3372KG → NP(PubMed:9164856)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 6767Missing in isoform 2. 1 PublicationVSP_015534Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05499 mRNA. Translation: AAC37707.1.
L76175 Genomic DNA. Translation: AAC37708.1.
BC089776 mRNA. Translation: AAH89776.1. Different initiation.
BC104712 mRNA. Translation: AAI04713.1.
PIRiA35444.
RefSeqiNP_001257715.1. NM_001270786.1. [P28492-1]
NP_001257716.1. NM_001270787.1.
NP_620259.2. NM_138904.2. [P28492-2]
XP_006240792.1. XM_006240730.2. [P28492-2]
UniGeneiRn.10202.

Genome annotation databases

EnsembliENSRNOT00000018737; ENSRNOP00000018737; ENSRNOG00000031612. [P28492-1]
GeneIDi192268.
KEGGirno:192268.
UCSCiRGD:620359. rat. [P28492-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05499 mRNA. Translation: AAC37707.1.
L76175 Genomic DNA. Translation: AAC37708.1.
BC089776 mRNA. Translation: AAH89776.1. Different initiation.
BC104712 mRNA. Translation: AAI04713.1.
PIRiA35444.
RefSeqiNP_001257715.1. NM_001270786.1. [P28492-1]
NP_001257716.1. NM_001270787.1.
NP_620259.2. NM_138904.2. [P28492-2]
XP_006240792.1. XM_006240730.2. [P28492-2]
UniGeneiRn.10202.

3D structure databases

ProteinModelPortaliP28492.
SMRiP28492. Positions 155-464.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PaxDbiP28492.
PRIDEiP28492.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000018737; ENSRNOP00000018737; ENSRNOG00000031612. [P28492-1]
GeneIDi192268.
KEGGirno:192268.
UCSCiRGD:620359. rat. [P28492-1]

Organism-specific databases

CTDi27165.
RGDi620359. Gls2.

Phylogenomic databases

eggNOGiCOG2066.
GeneTreeiENSGT00390000010463.
HOGENOMiHOG000216891.
HOVERGENiHBG005856.
InParanoidiP28492.
KOiK01425.
OMAiCVKPLTY.
OrthoDBiEOG7S4X5F.
PhylomeDBiP28492.
TreeFamiTF313359.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13074.
ReactomeiREACT_214662. Amino acid synthesis and interconversion (transamination).
REACT_227883. Glutamate Neurotransmitter Release Cycle.
SABIO-RKP28492.

Miscellaneous databases

NextBioi622960.
PROiP28492.

Gene expression databases

GenevestigatoriP28492.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
3.40.710.10. 1 hit.
HAMAPiMF_00313. Glutaminase.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR012338. Beta-lactam/transpept-like.
IPR015868. Glutaminase.
[Graphical view]
PANTHERiPTHR12544. PTHR12544. 1 hit.
PfamiPF12796. Ank_2. 1 hit.
PF04960. Glutaminase. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF56601. SSF56601. 1 hit.
TIGRFAMsiTIGR03814. Gln_ase. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Rat hepatic glutaminase: identification of the full coding sequence and characterization of a functional promoter."
    Chung-Bok M.I., Vincent N., Jhala U., Watford M.
    Biochem. J. 324:193-200(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 68-94.
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Liver.
  3. "Molecular cloning of a cDNA for rat hepatic glutaminase. Sequence similarity to kidney-type glutaminase."
    Smith E.M., Watford M.
    J. Biol. Chem. 265:10631-10636(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [MRNA] OF 147-602.
    Tissue: Liver.

Entry informationi

Entry nameiGLSL_RAT
AccessioniPrimary (citable) accession number: P28492
Secondary accession number(s): Q3MHS6, Q5FVU0, Q64606
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: August 30, 2005
Last modified: January 7, 2015
This is version 122 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.