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P28492

- GLSL_RAT

UniProt

P28492 - GLSL_RAT

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Protein

Glutaminase liver isoform, mitochondrial

Gene
Gls2, Ga
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production By similarity.UniRule annotation

Catalytic activityi

L-glutamine + H2O = L-glutamate + NH3.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei219 – 2191Substrate By similarity
Binding sitei268 – 2681Substrate By similarity
Binding sitei314 – 3141Substrate By similarity
Binding sitei321 – 3211Substrate By similarity
Binding sitei347 – 3471Substrate By similarity
Binding sitei399 – 3991Substrate By similarity
Binding sitei417 – 4171Substrate; via amide nitrogen By similarity

GO - Molecular functioni

  1. glutaminase activity Source: UniProtKB-EC

GO - Biological processi

  1. glutamine metabolic process Source: InterPro
  2. reactive oxygen species metabolic process Source: Ensembl
  3. regulation of apoptotic process Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13074.
ReactomeiREACT_214662. Amino acid synthesis and interconversion (transamination).
REACT_227883. Glutamate Neurotransmitter Release Cycle.
SABIO-RKP28492.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutaminase liver isoform, mitochondrial (EC:3.5.1.2)
Short name:
GLS
Alternative name(s):
L-glutaminase
L-glutamine amidohydrolase
Gene namesi
Name:Gls2
Synonyms:Ga
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 7

Organism-specific databases

RGDi620359. Gls2.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial matrix Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 1414Mitochondrion Reviewed predictionAdd
BLAST
Chaini15 – 602588Glutaminase liver isoform, mitochondrialUniRule annotationPRO_0000011627Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei253 – 2531N6-succinyllysine By similarity
Modified residuei279 – 2791N6-acetyllysine By similarity
Modified residuei284 – 2841N6-acetyllysine By similarity
Modified residuei329 – 3291N6-acetyllysine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP28492.
PRIDEiP28492.

Expressioni

Tissue specificityi

Liver specific.

Gene expression databases

GenevestigatoriP28492.

Interactioni

Subunit structurei

Interacts with the PDZ domain of the syntrophin SNTA1. Interacts with the PDZ domain of TAX1BP3 By similarity.

Structurei

3D structure databases

ProteinModelPortaliP28492.
SMRiP28492. Positions 155-464.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati518 – 55134ANK 1UniRule annotationAdd
BLAST
Repeati552 – 58534ANK 2UniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the glutaminase family.
Contains 2 ANK repeats.

Keywords - Domaini

ANK repeat, Repeat, Transit peptide

Phylogenomic databases

eggNOGiCOG2066.
GeneTreeiENSGT00390000010463.
HOGENOMiHOG000216891.
HOVERGENiHBG005856.
InParanoidiQ3MHS6.
KOiK01425.
OMAiCVKPLTY.
OrthoDBiEOG7S4X5F.
PhylomeDBiP28492.
TreeFamiTF313359.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
3.40.710.10. 1 hit.
HAMAPiMF_00313. Glutaminase.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR012338. Beta-lactam/transpept-like.
IPR015868. Glutaminase.
[Graphical view]
PANTHERiPTHR12544. PTHR12544. 1 hit.
PfamiPF12796. Ank_2. 1 hit.
PF04960. Glutaminase. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF56601. SSF56601. 1 hit.
TIGRFAMsiTIGR03814. Gln_ase. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P28492-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MRSMRALQNA LSRAGSHGQR GGWGHPSRGP LLGGGVRYYF GEAAAQGRGT    50
PHSHQPQHSD HDASNSGMLP RLGDLLFYTI AEGQERIPIH KFTTALKATG 100
LQTSDPRLQD CMSKMQRMVQ ESSSGGLLDR ELFQKCVSSN IVLLTQAFRK 150
KFVIPDFEEF TGHVDRIFED AKELTGGKVA AYIPHLAKSN PDLWGVSLCT 200
VDGQRHSVGH TKIPFCLQSC VKPLTYAISV STLGTDYVHK FVGKEPSGLR 250
YNKLSLNEEG IPHNPMVNAG AIVVSSLIKM DCNKAEKFDF VLQYLNKMAG 300
NEFMGFSNAT FQSEKETGDR NYAIGYYLKE KKCFPKGVDM MAALDLYFQL 350
CSVEVTCESG SVMAATLANG GICPITGESV LSAEAVRNTL SLMHSCGMYD 400
FSGQFAFHVG LPAKSAVSGA ILLVVPNVMG MMCLSPPLDK LGNSHRGISF 450
CQKLVSLFNF HNYDNLRHCA RKLDPRREGG EVRNKTVVNL LFAAYSGDVS 500
ALRRFALSAV DMEQKDYDSR TALHVAAAEG HIDVVKFLIE ACKVNPFVKD 550
RWGNIPLDDA VQFNHLEVVK LLQDYHDSYM LSETQAEVAA ETLSKENLES 600
MV 602
Length:602
Mass (Da):66,248
Last modified:August 30, 2005 - v3
Checksum:iF4AE3BD30626A00F
GO
Isoform 2 (identifier: P28492-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-67: Missing.

Show »
Length:535
Mass (Da):59,204
Checksum:iE608D53D78B3F7F1
GO

Sequence cautioni

The sequence AAH89776.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 6767Missing in isoform 2. VSP_015534Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti76 – 761L → V in AAC37708. 1 Publication
Sequence conflicti336 – 3372KG → NP1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J05499 mRNA. Translation: AAC37707.1.
L76175 Genomic DNA. Translation: AAC37708.1.
BC089776 mRNA. Translation: AAH89776.1. Different initiation.
BC104712 mRNA. Translation: AAI04713.1.
PIRiA35444.
RefSeqiNP_001257715.1. NM_001270786.1. [P28492-1]
NP_001257716.1. NM_001270787.1.
NP_620259.2. NM_138904.2. [P28492-2]
XP_006240792.1. XM_006240730.1. [P28492-2]
UniGeneiRn.10202.

Genome annotation databases

EnsembliENSRNOT00000018737; ENSRNOP00000018737; ENSRNOG00000031612. [P28492-1]
GeneIDi192268.
KEGGirno:192268.
UCSCiRGD:620359. rat. [P28492-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J05499 mRNA. Translation: AAC37707.1 .
L76175 Genomic DNA. Translation: AAC37708.1 .
BC089776 mRNA. Translation: AAH89776.1 . Different initiation.
BC104712 mRNA. Translation: AAI04713.1 .
PIRi A35444.
RefSeqi NP_001257715.1. NM_001270786.1. [P28492-1 ]
NP_001257716.1. NM_001270787.1.
NP_620259.2. NM_138904.2. [P28492-2 ]
XP_006240792.1. XM_006240730.1. [P28492-2 ]
UniGenei Rn.10202.

3D structure databases

ProteinModelPortali P28492.
SMRi P28492. Positions 155-464.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi P28492.
PRIDEi P28492.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000018737 ; ENSRNOP00000018737 ; ENSRNOG00000031612 . [P28492-1 ]
GeneIDi 192268.
KEGGi rno:192268.
UCSCi RGD:620359. rat. [P28492-1 ]

Organism-specific databases

CTDi 27165.
RGDi 620359. Gls2.

Phylogenomic databases

eggNOGi COG2066.
GeneTreei ENSGT00390000010463.
HOGENOMi HOG000216891.
HOVERGENi HBG005856.
InParanoidi Q3MHS6.
KOi K01425.
OMAi CVKPLTY.
OrthoDBi EOG7S4X5F.
PhylomeDBi P28492.
TreeFami TF313359.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-13074.
Reactomei REACT_214662. Amino acid synthesis and interconversion (transamination).
REACT_227883. Glutamate Neurotransmitter Release Cycle.
SABIO-RK P28492.

Miscellaneous databases

NextBioi 622960.
PROi P28492.

Gene expression databases

Genevestigatori P28492.

Family and domain databases

Gene3Di 1.25.40.20. 1 hit.
3.40.710.10. 1 hit.
HAMAPi MF_00313. Glutaminase.
InterProi IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR012338. Beta-lactam/transpept-like.
IPR015868. Glutaminase.
[Graphical view ]
PANTHERi PTHR12544. PTHR12544. 1 hit.
Pfami PF12796. Ank_2. 1 hit.
PF04960. Glutaminase. 1 hit.
[Graphical view ]
SMARTi SM00248. ANK. 2 hits.
[Graphical view ]
SUPFAMi SSF48403. SSF48403. 1 hit.
SSF56601. SSF56601. 1 hit.
TIGRFAMsi TIGR03814. Gln_ase. 1 hit.
PROSITEi PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Rat hepatic glutaminase: identification of the full coding sequence and characterization of a functional promoter."
    Chung-Bok M.I., Vincent N., Jhala U., Watford M.
    Biochem. J. 324:193-200(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 68-94.
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Liver.
  3. "Molecular cloning of a cDNA for rat hepatic glutaminase. Sequence similarity to kidney-type glutaminase."
    Smith E.M., Watford M.
    J. Biol. Chem. 265:10631-10636(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [MRNA] OF 147-602.
    Tissue: Liver.

Entry informationi

Entry nameiGLSL_RAT
AccessioniPrimary (citable) accession number: P28492
Secondary accession number(s): Q3MHS6, Q5FVU0, Q64606
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: August 30, 2005
Last modified: September 3, 2014
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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