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P28492

- GLSL_RAT

UniProt

P28492 - GLSL_RAT

Protein

Glutaminase liver isoform, mitochondrial

Gene

Gls2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 3 (30 Aug 2005)
      Previous versions | rss
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    Functioni

    Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production By similarity.By similarity

    Catalytic activityi

    L-glutamine + H2O = L-glutamate + NH3.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei219 – 2191SubstrateBy similarity
    Binding sitei268 – 2681SubstrateBy similarity
    Binding sitei314 – 3141SubstrateBy similarity
    Binding sitei321 – 3211SubstrateBy similarity
    Binding sitei347 – 3471SubstrateBy similarity
    Binding sitei399 – 3991SubstrateBy similarity
    Binding sitei417 – 4171Substrate; via amide nitrogenBy similarity

    GO - Molecular functioni

    1. glutaminase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glutamine metabolic process Source: InterPro
    2. reactive oxygen species metabolic process Source: Ensembl
    3. regulation of apoptotic process Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-13074.
    ReactomeiREACT_214662. Amino acid synthesis and interconversion (transamination).
    REACT_227883. Glutamate Neurotransmitter Release Cycle.
    SABIO-RKP28492.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutaminase liver isoform, mitochondrial (EC:3.5.1.2)
    Short name:
    GLS
    Alternative name(s):
    L-glutaminase
    L-glutamine amidohydrolase
    Gene namesi
    Name:Gls2
    Synonyms:Ga
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 7

    Organism-specific databases

    RGDi620359. Gls2.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial matrix Source: RGD

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 1414MitochondrionSequence AnalysisAdd
    BLAST
    Chaini15 – 602588Glutaminase liver isoform, mitochondrialPRO_0000011627Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei253 – 2531N6-succinyllysineBy similarity
    Modified residuei279 – 2791N6-acetyllysineBy similarity
    Modified residuei284 – 2841N6-acetyllysineBy similarity
    Modified residuei329 – 3291N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiP28492.
    PRIDEiP28492.

    Expressioni

    Tissue specificityi

    Liver specific.

    Gene expression databases

    GenevestigatoriP28492.

    Interactioni

    Subunit structurei

    Interacts with the PDZ domain of the syntrophin SNTA1. Interacts with the PDZ domain of TAX1BP3 By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP28492.
    SMRiP28492. Positions 155-464.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati518 – 55134ANK 1Add
    BLAST
    Repeati552 – 58534ANK 2Add
    BLAST

    Sequence similaritiesi

    Belongs to the glutaminase family.Curated
    Contains 2 ANK repeats.Curated

    Keywords - Domaini

    ANK repeat, Repeat, Transit peptide

    Phylogenomic databases

    eggNOGiCOG2066.
    GeneTreeiENSGT00390000010463.
    HOGENOMiHOG000216891.
    HOVERGENiHBG005856.
    InParanoidiQ3MHS6.
    KOiK01425.
    OMAiCVKPLTY.
    OrthoDBiEOG7S4X5F.
    PhylomeDBiP28492.
    TreeFamiTF313359.

    Family and domain databases

    Gene3Di1.25.40.20. 1 hit.
    3.40.710.10. 1 hit.
    HAMAPiMF_00313. Glutaminase.
    InterProiIPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR012338. Beta-lactam/transpept-like.
    IPR015868. Glutaminase.
    [Graphical view]
    PANTHERiPTHR12544. PTHR12544. 1 hit.
    PfamiPF12796. Ank_2. 1 hit.
    PF04960. Glutaminase. 1 hit.
    [Graphical view]
    SMARTiSM00248. ANK. 2 hits.
    [Graphical view]
    SUPFAMiSSF48403. SSF48403. 1 hit.
    SSF56601. SSF56601. 1 hit.
    TIGRFAMsiTIGR03814. Gln_ase. 1 hit.
    PROSITEiPS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P28492-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRSMRALQNA LSRAGSHGQR GGWGHPSRGP LLGGGVRYYF GEAAAQGRGT    50
    PHSHQPQHSD HDASNSGMLP RLGDLLFYTI AEGQERIPIH KFTTALKATG 100
    LQTSDPRLQD CMSKMQRMVQ ESSSGGLLDR ELFQKCVSSN IVLLTQAFRK 150
    KFVIPDFEEF TGHVDRIFED AKELTGGKVA AYIPHLAKSN PDLWGVSLCT 200
    VDGQRHSVGH TKIPFCLQSC VKPLTYAISV STLGTDYVHK FVGKEPSGLR 250
    YNKLSLNEEG IPHNPMVNAG AIVVSSLIKM DCNKAEKFDF VLQYLNKMAG 300
    NEFMGFSNAT FQSEKETGDR NYAIGYYLKE KKCFPKGVDM MAALDLYFQL 350
    CSVEVTCESG SVMAATLANG GICPITGESV LSAEAVRNTL SLMHSCGMYD 400
    FSGQFAFHVG LPAKSAVSGA ILLVVPNVMG MMCLSPPLDK LGNSHRGISF 450
    CQKLVSLFNF HNYDNLRHCA RKLDPRREGG EVRNKTVVNL LFAAYSGDVS 500
    ALRRFALSAV DMEQKDYDSR TALHVAAAEG HIDVVKFLIE ACKVNPFVKD 550
    RWGNIPLDDA VQFNHLEVVK LLQDYHDSYM LSETQAEVAA ETLSKENLES 600
    MV 602
    Length:602
    Mass (Da):66,248
    Last modified:August 30, 2005 - v3
    Checksum:iF4AE3BD30626A00F
    GO
    Isoform 2 (identifier: P28492-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-67: Missing.

    Show »
    Length:535
    Mass (Da):59,204
    Checksum:iE608D53D78B3F7F1
    GO

    Sequence cautioni

    The sequence AAH89776.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti76 – 761L → V in AAC37708. (PubMed:9164856)Curated
    Sequence conflicti336 – 3372KG → NP(PubMed:9164856)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 6767Missing in isoform 2. 1 PublicationVSP_015534Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05499 mRNA. Translation: AAC37707.1.
    L76175 Genomic DNA. Translation: AAC37708.1.
    BC089776 mRNA. Translation: AAH89776.1. Different initiation.
    BC104712 mRNA. Translation: AAI04713.1.
    PIRiA35444.
    RefSeqiNP_001257715.1. NM_001270786.1. [P28492-1]
    NP_001257716.1. NM_001270787.1.
    NP_620259.2. NM_138904.2. [P28492-2]
    XP_006240792.1. XM_006240730.1. [P28492-2]
    UniGeneiRn.10202.

    Genome annotation databases

    EnsembliENSRNOT00000018737; ENSRNOP00000018737; ENSRNOG00000031612. [P28492-1]
    GeneIDi192268.
    KEGGirno:192268.
    UCSCiRGD:620359. rat. [P28492-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05499 mRNA. Translation: AAC37707.1 .
    L76175 Genomic DNA. Translation: AAC37708.1 .
    BC089776 mRNA. Translation: AAH89776.1 . Different initiation.
    BC104712 mRNA. Translation: AAI04713.1 .
    PIRi A35444.
    RefSeqi NP_001257715.1. NM_001270786.1. [P28492-1 ]
    NP_001257716.1. NM_001270787.1.
    NP_620259.2. NM_138904.2. [P28492-2 ]
    XP_006240792.1. XM_006240730.1. [P28492-2 ]
    UniGenei Rn.10202.

    3D structure databases

    ProteinModelPortali P28492.
    SMRi P28492. Positions 155-464.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi P28492.
    PRIDEi P28492.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000018737 ; ENSRNOP00000018737 ; ENSRNOG00000031612 . [P28492-1 ]
    GeneIDi 192268.
    KEGGi rno:192268.
    UCSCi RGD:620359. rat. [P28492-1 ]

    Organism-specific databases

    CTDi 27165.
    RGDi 620359. Gls2.

    Phylogenomic databases

    eggNOGi COG2066.
    GeneTreei ENSGT00390000010463.
    HOGENOMi HOG000216891.
    HOVERGENi HBG005856.
    InParanoidi Q3MHS6.
    KOi K01425.
    OMAi CVKPLTY.
    OrthoDBi EOG7S4X5F.
    PhylomeDBi P28492.
    TreeFami TF313359.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-13074.
    Reactomei REACT_214662. Amino acid synthesis and interconversion (transamination).
    REACT_227883. Glutamate Neurotransmitter Release Cycle.
    SABIO-RK P28492.

    Miscellaneous databases

    NextBioi 622960.
    PROi P28492.

    Gene expression databases

    Genevestigatori P28492.

    Family and domain databases

    Gene3Di 1.25.40.20. 1 hit.
    3.40.710.10. 1 hit.
    HAMAPi MF_00313. Glutaminase.
    InterProi IPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR012338. Beta-lactam/transpept-like.
    IPR015868. Glutaminase.
    [Graphical view ]
    PANTHERi PTHR12544. PTHR12544. 1 hit.
    Pfami PF12796. Ank_2. 1 hit.
    PF04960. Glutaminase. 1 hit.
    [Graphical view ]
    SMARTi SM00248. ANK. 2 hits.
    [Graphical view ]
    SUPFAMi SSF48403. SSF48403. 1 hit.
    SSF56601. SSF56601. 1 hit.
    TIGRFAMsi TIGR03814. Gln_ase. 1 hit.
    PROSITEi PS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Rat hepatic glutaminase: identification of the full coding sequence and characterization of a functional promoter."
      Chung-Bok M.I., Vincent N., Jhala U., Watford M.
      Biochem. J. 324:193-200(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 68-94.
      Tissue: Liver.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Liver.
    3. "Molecular cloning of a cDNA for rat hepatic glutaminase. Sequence similarity to kidney-type glutaminase."
      Smith E.M., Watford M.
      J. Biol. Chem. 265:10631-10636(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [MRNA] OF 147-602.
      Tissue: Liver.

    Entry informationi

    Entry nameiGLSL_RAT
    AccessioniPrimary (citable) accession number: P28492
    Secondary accession number(s): Q3MHS6, Q5FVU0, Q64606
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: August 30, 2005
    Last modified: October 1, 2014
    This is version 120 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3