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P28492 (GLSL_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutaminase liver isoform, mitochondrial

Short name=GLS
EC=3.5.1.2
Alternative name(s):
L-glutaminase
L-glutamine amidohydrolase
Gene names
Name:Gls2
Synonyms:Ga
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length602 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production By similarity. HAMAP-Rule MF_00313

Catalytic activity

L-glutamine + H2O = L-glutamate + NH3. HAMAP-Rule MF_00313

Subunit structure

Interacts with the PDZ domain of the syntrophin SNTA1. Interacts with the PDZ domain of TAX1BP3 By similarity.

Subcellular location

Mitochondrion HAMAP-Rule MF_00313.

Tissue specificity

Liver specific.

Sequence similarities

Belongs to the glutaminase family.

Contains 2 ANK repeats.

Sequence caution

The sequence AAH89776.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
   Cellular componentMitochondrion
   Coding sequence diversityAlternative splicing
   DomainANK repeat
Repeat
Transit peptide
   Molecular functionHydrolase
   PTMAcetylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglutamine metabolic process

Inferred from electronic annotation. Source: InterPro

reactive oxygen species metabolic process

Inferred from electronic annotation. Source: Ensembl

regulation of apoptotic process

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentmitochondrial matrix

Traceable author statement Ref.1. Source: RGD

   Molecular_functionglutaminase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P28492-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P28492-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-67: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 1414Mitochondrion Potential
Chain15 – 602588Glutaminase liver isoform, mitochondrial HAMAP-Rule MF_00313
PRO_0000011627

Regions

Repeat518 – 55134ANK 1 HAMAP-Rule MF_00313
Repeat552 – 58534ANK 2 HAMAP-Rule MF_00313

Sites

Binding site2191Substrate By similarity
Binding site2681Substrate By similarity
Binding site3141Substrate By similarity
Binding site3211Substrate By similarity
Binding site3471Substrate By similarity
Binding site3991Substrate By similarity
Binding site4171Substrate; via amide nitrogen By similarity

Amino acid modifications

Modified residue2531N6-succinyllysine By similarity
Modified residue2791N6-acetyllysine By similarity
Modified residue2841N6-acetyllysine By similarity
Modified residue3291N6-acetyllysine By similarity

Natural variations

Alternative sequence1 – 6767Missing in isoform 2.
VSP_015534

Experimental info

Sequence conflict761L → V in AAC37708. Ref.1
Sequence conflict336 – 3372KG → NP Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified August 30, 2005. Version 3.
Checksum: F4AE3BD30626A00F

FASTA60266,248
        10         20         30         40         50         60 
MRSMRALQNA LSRAGSHGQR GGWGHPSRGP LLGGGVRYYF GEAAAQGRGT PHSHQPQHSD 

        70         80         90        100        110        120 
HDASNSGMLP RLGDLLFYTI AEGQERIPIH KFTTALKATG LQTSDPRLQD CMSKMQRMVQ 

       130        140        150        160        170        180 
ESSSGGLLDR ELFQKCVSSN IVLLTQAFRK KFVIPDFEEF TGHVDRIFED AKELTGGKVA 

       190        200        210        220        230        240 
AYIPHLAKSN PDLWGVSLCT VDGQRHSVGH TKIPFCLQSC VKPLTYAISV STLGTDYVHK 

       250        260        270        280        290        300 
FVGKEPSGLR YNKLSLNEEG IPHNPMVNAG AIVVSSLIKM DCNKAEKFDF VLQYLNKMAG 

       310        320        330        340        350        360 
NEFMGFSNAT FQSEKETGDR NYAIGYYLKE KKCFPKGVDM MAALDLYFQL CSVEVTCESG 

       370        380        390        400        410        420 
SVMAATLANG GICPITGESV LSAEAVRNTL SLMHSCGMYD FSGQFAFHVG LPAKSAVSGA 

       430        440        450        460        470        480 
ILLVVPNVMG MMCLSPPLDK LGNSHRGISF CQKLVSLFNF HNYDNLRHCA RKLDPRREGG 

       490        500        510        520        530        540 
EVRNKTVVNL LFAAYSGDVS ALRRFALSAV DMEQKDYDSR TALHVAAAEG HIDVVKFLIE 

       550        560        570        580        590        600 
ACKVNPFVKD RWGNIPLDDA VQFNHLEVVK LLQDYHDSYM LSETQAEVAA ETLSKENLES 


MV 

« Hide

Isoform 2 [UniParc].

Checksum: E608D53D78B3F7F1
Show »

FASTA53559,204

References

« Hide 'large scale' references
[1]"Rat hepatic glutaminase: identification of the full coding sequence and characterization of a functional promoter."
Chung-Bok M.I., Vincent N., Jhala U., Watford M.
Biochem. J. 324:193-200(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 68-94.
Tissue: Liver.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Liver.
[3]"Molecular cloning of a cDNA for rat hepatic glutaminase. Sequence similarity to kidney-type glutaminase."
Smith E.M., Watford M.
J. Biol. Chem. 265:10631-10636(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [MRNA] OF 147-602.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J05499 mRNA. Translation: AAC37707.1.
L76175 Genomic DNA. Translation: AAC37708.1.
BC089776 mRNA. Translation: AAH89776.1. Different initiation.
BC104712 mRNA. Translation: AAI04713.1.
PIRA35444.
RefSeqNP_001257715.1. NM_001270786.1.
NP_001257716.1. NM_001270787.1.
NP_620259.2. NM_138904.2.
XP_006240792.1. XM_006240730.1.
UniGeneRn.10202.

3D structure databases

ProteinModelPortalP28492.
SMRP28492. Positions 155-464.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbP28492.
PRIDEP28492.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000018737; ENSRNOP00000018737; ENSRNOG00000031612. [P28492-1]
GeneID192268.
KEGGrno:192268.
UCSCRGD:620359. rat. [P28492-1]

Organism-specific databases

CTD27165.
RGD620359. Gls2.

Phylogenomic databases

eggNOGCOG2066.
GeneTreeENSGT00390000010463.
HOGENOMHOG000216891.
HOVERGENHBG005856.
InParanoidQ3MHS6.
KOK01425.
OMAEGGEVRN.
OrthoDBEOG7S4X5F.
PhylomeDBP28492.
TreeFamTF313359.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-13074.
SABIO-RKP28492.

Gene expression databases

GenevestigatorP28492.

Family and domain databases

Gene3D1.25.40.20. 1 hit.
3.40.710.10. 1 hit.
HAMAPMF_00313. Glutaminase.
InterProIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR012338. Beta-lactam/transpept-like.
IPR015868. Glutaminase.
[Graphical view]
PANTHERPTHR12544. PTHR12544. 1 hit.
PfamPF12796. Ank_2. 1 hit.
PF04960. Glutaminase. 1 hit.
[Graphical view]
SMARTSM00248. ANK. 2 hits.
[Graphical view]
SUPFAMSSF48403. SSF48403. 1 hit.
SSF56601. SSF56601. 1 hit.
TIGRFAMsTIGR03814. Gln_ase. 1 hit.
PROSITEPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio622960.
PROP28492.

Entry information

Entry nameGLSL_RAT
AccessionPrimary (citable) accession number: P28492
Secondary accession number(s): Q3MHS6, Q5FVU0, Q64606
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: August 30, 2005
Last modified: April 16, 2014
This is version 117 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families