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Protein

Glutaminase liver isoform, mitochondrial

Gene

Gls2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production (By similarity).By similarity

Catalytic activityi

L-glutamine + H2O = L-glutamate + NH3.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei219SubstrateBy similarity1
Binding sitei268SubstrateBy similarity1
Binding sitei314SubstrateBy similarity1
Binding sitei321SubstrateBy similarity1
Binding sitei347SubstrateBy similarity1
Binding sitei399SubstrateBy similarity1
Binding sitei417Substrate; via amide nitrogenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13074.
SABIO-RKiP28492.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutaminase liver isoform, mitochondrial (EC:3.5.1.2)
Short name:
GLS
Alternative name(s):
L-glutaminase
L-glutamine amidohydrolase
Gene namesi
Name:Gls2
Synonyms:Ga
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620359. Gls2.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: RGD

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 14MitochondrionSequence analysisAdd BLAST14
ChainiPRO_000001162715 – 602Glutaminase liver isoform, mitochondrialAdd BLAST588

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei253N6-succinyllysineBy similarity1
Modified residuei279N6-acetyllysineBy similarity1
Modified residuei284N6-acetyllysineBy similarity1
Modified residuei329N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP28492.
PRIDEiP28492.

Expressioni

Tissue specificityi

Liver specific.

Gene expression databases

GenevisibleiP28492. RN.

Interactioni

Subunit structurei

Interacts with the PDZ domain of the syntrophin SNTA1. Interacts with the PDZ domain of TAX1BP3 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000018737.

Structurei

3D structure databases

ProteinModelPortaliP28492.
SMRiP28492.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati518 – 551ANK 1Add BLAST34
Repeati552 – 585ANK 2Add BLAST34

Sequence similaritiesi

Belongs to the glutaminase family.Curated

Keywords - Domaini

ANK repeat, Repeat, Transit peptide

Phylogenomic databases

eggNOGiKOG0506. Eukaryota.
COG2066. LUCA.
HOGENOMiHOG000216891.
HOVERGENiHBG005856.
InParanoidiP28492.
KOiK01425.
OrthoDBiEOG091G03YU.
PhylomeDBiP28492.
TreeFamiTF313359.

Family and domain databases

CDDicd00204. ANK. 1 hit.
Gene3Di1.25.40.20. 1 hit.
HAMAPiMF_00313. Glutaminase. 1 hit.
InterProiView protein in InterPro
IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR012338. Beta-lactam/transpept-like.
IPR015868. Glutaminase.
PANTHERiPTHR12544. PTHR12544. 1 hit.
PfamiView protein in Pfam
PF12796. Ank_2. 1 hit.
PF04960. Glutaminase. 1 hit.
SMARTiView protein in SMART
SM00248. ANK. 2 hits.
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF56601. SSF56601. 1 hit.
TIGRFAMsiTIGR03814. Gln_ase. 1 hit.
PROSITEiView protein in PROSITE
PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P28492-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRSMRALQNA LSRAGSHGQR GGWGHPSRGP LLGGGVRYYF GEAAAQGRGT
60 70 80 90 100
PHSHQPQHSD HDASNSGMLP RLGDLLFYTI AEGQERIPIH KFTTALKATG
110 120 130 140 150
LQTSDPRLQD CMSKMQRMVQ ESSSGGLLDR ELFQKCVSSN IVLLTQAFRK
160 170 180 190 200
KFVIPDFEEF TGHVDRIFED AKELTGGKVA AYIPHLAKSN PDLWGVSLCT
210 220 230 240 250
VDGQRHSVGH TKIPFCLQSC VKPLTYAISV STLGTDYVHK FVGKEPSGLR
260 270 280 290 300
YNKLSLNEEG IPHNPMVNAG AIVVSSLIKM DCNKAEKFDF VLQYLNKMAG
310 320 330 340 350
NEFMGFSNAT FQSEKETGDR NYAIGYYLKE KKCFPKGVDM MAALDLYFQL
360 370 380 390 400
CSVEVTCESG SVMAATLANG GICPITGESV LSAEAVRNTL SLMHSCGMYD
410 420 430 440 450
FSGQFAFHVG LPAKSAVSGA ILLVVPNVMG MMCLSPPLDK LGNSHRGISF
460 470 480 490 500
CQKLVSLFNF HNYDNLRHCA RKLDPRREGG EVRNKTVVNL LFAAYSGDVS
510 520 530 540 550
ALRRFALSAV DMEQKDYDSR TALHVAAAEG HIDVVKFLIE ACKVNPFVKD
560 570 580 590 600
RWGNIPLDDA VQFNHLEVVK LLQDYHDSYM LSETQAEVAA ETLSKENLES

MV
Length:602
Mass (Da):66,248
Last modified:August 30, 2005 - v3
Checksum:iF4AE3BD30626A00F
GO
Isoform 2 (identifier: P28492-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-67: Missing.

Show »
Length:535
Mass (Da):59,204
Checksum:iE608D53D78B3F7F1
GO

Sequence cautioni

The sequence AAH89776 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti76L → V in AAC37708 (PubMed:9164856).Curated1
Sequence conflicti336 – 337KG → NP (PubMed:9164856).Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0155341 – 67Missing in isoform 2. 1 PublicationAdd BLAST67

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05499 mRNA. Translation: AAC37707.1.
L76175 Genomic DNA. Translation: AAC37708.1.
BC089776 mRNA. Translation: AAH89776.1. Different initiation.
BC104712 mRNA. Translation: AAI04713.1.
PIRiA35444.
RefSeqiNP_001257715.1. NM_001270786.1. [P28492-1]
NP_001257716.1. NM_001270787.1.
NP_620259.2. NM_138904.2. [P28492-2]
XP_006240792.1. XM_006240730.2. [P28492-2]
UniGeneiRn.10202.

Genome annotation databases

GeneIDi192268.
KEGGirno:192268.
UCSCiRGD:620359. rat. [P28492-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGLSL_RAT
AccessioniPrimary (citable) accession number: P28492
Secondary accession number(s): Q3MHS6, Q5FVU0, Q64606
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: August 30, 2005
Last modified: August 30, 2017
This is version 141 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families