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Protein

Calreticulin

Gene

CALR

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export (By similarity). Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis.By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi26Calcium; via carbonyl oxygenBy similarity1
Metal bindingi62Calcium; via carbonyl oxygenBy similarity1
Metal bindingi64Calcium; via carbonyl oxygenBy similarity1
Binding sitei109CarbohydrateBy similarity1
Binding sitei111CarbohydrateBy similarity1
Binding sitei128CarbohydrateBy similarity1
Binding sitei135CarbohydrateBy similarity1
Binding sitei317CarbohydrateBy similarity1
Metal bindingi328CalciumBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Ligandi

Calcium, Lectin, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-SSC-1236974. ER-Phagosome pathway.
R-SSC-3000480. Scavenging by Class A Receptors.
R-SSC-901042. Calnexin/calreticulin cycle.
R-SSC-983170. Antigen Presentation: Folding, assembly and peptide loading of class I MHC.

Names & Taxonomyi

Protein namesi
Recommended name:
Calreticulin
Alternative name(s):
CRP55
Calregulin
Endoplasmic reticulum resident protein 60
Short name:
ERp60
HACBP
Gene namesi
Name:CALR
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Chromosome 2

Subcellular locationi

  • Endoplasmic reticulum lumen 1 Publication
  • Sarcoplasmic reticulum lumen 1 Publication
  • Cytoplasmperinuclear region 1 Publication
  • Membrane 1 Publication

  • Note: During oocyte maturation and after parthenogenetic activation accumulates in the plasma membrane region. In pronuclear and early cleaved embryos localizes weakly to cytoplasm around nucleus and more strongly in the region near the cortex (PubMed:20222029).1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane, Sarcoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 171 PublicationAdd BLAST17
ChainiPRO_000000417518 – 417CalreticulinAdd BLAST400

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi137 ↔ 163By similarity
Modified residuei159N6-acetyllysineBy similarity1
Modified residuei209N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Disulfide bond

Proteomic databases

PaxDbiP28491.
PeptideAtlasiP28491.
PRIDEiP28491.

Expressioni

Tissue specificityi

In blastocyst expressed in all blastomeres (at protein level). In embryos, expressed in spleen, kidney, liver, fat, muscle, ovary, granulosa cells and cumulus cells.1 Publication

Developmental stagei

Expressed in immature GV-stage oocytes, mature MII-stage oocytes, parthenogenetically activated MII-stage oocytes and in pronuclear embryos (at protein level). During in vitro oocyte maturation, expression initially increases after 12 hours of culture, followed by a strong decline at 30 hours (MI stage) and 44 hours (MII stage). Expression remains constant in MII-stage oocytes after parthenogenetic activation, increasing in two- and four-cell parthenotes. Not detected in blastocyst stage embryos.1 Publication

Gene expression databases

BgeeiENSSSCG00000013746.
GenevisibleiP28491. SS.

Interactioni

Subunit structurei

Monomer. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Interacts with GABARAP, NR3C1, PDIA3/ERp57 and TRIM21. Interacts with PPIB and SPACA9 (By similarity).By similarity

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000014615.

Structurei

3D structure databases

ProteinModelPortaliP28491.
SMRiP28491.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati191 – 2021-1Add BLAST12
Repeati210 – 2211-2Add BLAST12
Repeati227 – 2381-3Add BLAST12
Repeati244 – 2551-4Add BLAST12
Repeati259 – 2692-1Add BLAST11
Repeati273 – 2832-2Add BLAST11
Repeati287 – 2972-3Add BLAST11

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni18 – 197N-domainAdd BLAST180
Regioni191 – 2554 X approximate repeatsAdd BLAST65
Regioni198 – 308P-domainAdd BLAST111
Regioni237 – 270Interaction with PPIBBy similarityAdd BLAST34
Regioni259 – 2973 X approximate repeatsAdd BLAST39
Regioni309 – 417C-domainAdd BLAST109

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi414 – 417Prevents secretion from ERPROSITE-ProRule annotation4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi351 – 407Asp/Glu/Lys-richAdd BLAST57

Domaini

Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity (By similarity).By similarity
The interaction with glycans occurs through a binding site in the globular lectin domain.By similarity
The zinc binding sites are localized to the N-domain.By similarity

Sequence similaritiesi

Belongs to the calreticulin family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG0674. Eukaryota.
ENOG410XRR7. LUCA.
GeneTreeiENSGT00430000030841.
HOGENOMiHOG000192435.
InParanoidiP28491.
KOiK08057.
OMAiIPNPEYM.
OrthoDBiEOG091G0AP1.
TreeFamiTF338438.

Family and domain databases

Gene3Di2.10.250.10. 1 hit.
2.60.120.200. 2 hits.
InterProiIPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009169. Calreticulin.
IPR009033. Calreticulin/calnexin_P_dom.
IPR013320. ConA-like_dom.
[Graphical view]
PANTHERiPTHR11073. PTHR11073. 1 hit.
PfamiPF00262. Calreticulin. 2 hits.
[Graphical view]
PIRSFiPIRSF002356. Calreticulin. 1 hit.
PRINTSiPR00626. CALRETICULIN.
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF63887. SSF63887. 1 hit.
PROSITEiPS00803. CALRETICULIN_1. 1 hit.
PS00804. CALRETICULIN_2. 1 hit.
PS00805. CALRETICULIN_REPEAT. 3 hits.
PS00014. ER_TARGET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28491-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLPVPLLLG LVGLAAAEPT IYFKEQFLDG DGWTDRWIES KHKPDFGRFV
60 70 80 90 100
LSSGKFYGDQ EKDKGLQTSQ DARFYALSAR FEPFSNKGQT LVVQFTVKHE
110 120 130 140 150
QNIDCGGGYV KLFPDGLDQT DMHGDSEYNI MFGPDICGPG TKKVHVIFNY
160 170 180 190 200
KGKNVLINKD IRCKDDEFTH LYTLIVRPDN TYEVKIDNSQ VESGSLEDDW
210 220 230 240 250
DFLPPKKIKD PDAVKPEDWD ERAKIDDPTD SKPEDWDKPE HIPDPDAKKP
260 270 280 290 300
EDWDEEMDGE WEPPVIQNPE YKGEWKPRQI DNPDYKGTWI HPEIDNPEYS
310 320 330 340 350
PDSNIYAYEN FAVLGLDLWQ VKSGTIFDNF LITNDEAYAE EFGNETWGVT
360 370 380 390 400
KAAEKQMKDK QDEEQRLKEE EEEKKRKEEE EVDKEDEEDK DEDEEEEDEK
410
EEEEEEDAAA GQAKDEL
Length:417
Mass (Da):48,288
Last modified:May 16, 2012 - v3
Checksum:i31A9E4CC95F2D54F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti15A → V in CAA23142 (PubMed:8672129).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GQ984146 mRNA. Translation: ADD52600.1.
CU463133 Genomic DNA. No translation available.
F14591 mRNA. Translation: CAA23142.1.
PIRiB33208.
RefSeqiNP_001167604.1. NM_001174133.1.
UniGeneiSsc.48945.

Genome annotation databases

EnsembliENSSSCT00000015020; ENSSSCP00000014615; ENSSSCG00000013746.
GeneIDi100381266.
KEGGissc:100381266.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GQ984146 mRNA. Translation: ADD52600.1.
CU463133 Genomic DNA. No translation available.
F14591 mRNA. Translation: CAA23142.1.
PIRiB33208.
RefSeqiNP_001167604.1. NM_001174133.1.
UniGeneiSsc.48945.

3D structure databases

ProteinModelPortaliP28491.
SMRiP28491.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000014615.

Proteomic databases

PaxDbiP28491.
PeptideAtlasiP28491.
PRIDEiP28491.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSSSCT00000015020; ENSSSCP00000014615; ENSSSCG00000013746.
GeneIDi100381266.
KEGGissc:100381266.

Organism-specific databases

CTDi811.

Phylogenomic databases

eggNOGiKOG0674. Eukaryota.
ENOG410XRR7. LUCA.
GeneTreeiENSGT00430000030841.
HOGENOMiHOG000192435.
InParanoidiP28491.
KOiK08057.
OMAiIPNPEYM.
OrthoDBiEOG091G0AP1.
TreeFamiTF338438.

Enzyme and pathway databases

ReactomeiR-SSC-1236974. ER-Phagosome pathway.
R-SSC-3000480. Scavenging by Class A Receptors.
R-SSC-901042. Calnexin/calreticulin cycle.
R-SSC-983170. Antigen Presentation: Folding, assembly and peptide loading of class I MHC.

Gene expression databases

BgeeiENSSSCG00000013746.
GenevisibleiP28491. SS.

Family and domain databases

Gene3Di2.10.250.10. 1 hit.
2.60.120.200. 2 hits.
InterProiIPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009169. Calreticulin.
IPR009033. Calreticulin/calnexin_P_dom.
IPR013320. ConA-like_dom.
[Graphical view]
PANTHERiPTHR11073. PTHR11073. 1 hit.
PfamiPF00262. Calreticulin. 2 hits.
[Graphical view]
PIRSFiPIRSF002356. Calreticulin. 1 hit.
PRINTSiPR00626. CALRETICULIN.
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF63887. SSF63887. 1 hit.
PROSITEiPS00803. CALRETICULIN_1. 1 hit.
PS00804. CALRETICULIN_2. 1 hit.
PS00805. CALRETICULIN_REPEAT. 3 hits.
PS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCALR_PIG
AccessioniPrimary (citable) accession number: P28491
Secondary accession number(s): D4N5N1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: May 16, 2012
Last modified: November 30, 2016
This is version 113 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.