P28475 (S6PD_MALDO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADP-dependent D-sorbitol-6-phosphate dehydrogenase
EC=1.1.1.200

Alternative name(s):
Aldose-6-phosphate reductase [NADPH]
NADP-S6PDH

Gene names

Name:

S6PDH

Organism

Malus domestica (Apple) (Pyrus malus)

Taxonomic identifier

3750 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Rosales › Rosaceae › Maloideae › Maleae › Malus

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Synthesizes sorbitol-6-phosphate, a key intermediate in the synthesis of sorbitol which is a major photosynthetic product in many members of the Rosaceae family.
Catalytic activity	D-sorbitol 6-phosphate + NADP+ = D-glucose 6-phosphate + NADPH.
Sequence similarities	Belongs to the aldo/keto reductase family.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Molecular function	aldose-6-phosphate reductase (NADPH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 310	310	NADP-dependent D-sorbitol-6-phosphate dehydrogenase		PRO_0000124657
Regions
	Nucleotide binding	210 – 272	63	NADP By similarity
Sites
	Active site	48	1	Proton donor By similarity
	Binding site	108	1	Substrate By similarity
	Site	77	1	Lowers pKa of active site Tyr By similarity

Sequences

	Sequence		Length	Mass (Da)
	P28475 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: DECCAD94BF5E6D9F	FASTA	310	34,905
	10 20 30 40 50 60 MSTVTLSSGY EMPVIGLGLW RLEKDELKEV ILNAIKIGYR HFDCAAHYKS EADVGEALAE 70 80 90 100 110 120 AFKTGLVKRE ELFITTKIWN SDHGHVVEAC KNSLEKLQID YLDLYLVHYP MPTKHNAIGK 130 140 150 160 170 180 TASLLGEDKV LDIDVTISLQ QTWEGMEKTV SLGLVRSIGL SNYELFLTRD CLAYSKIKPA 190 200 210 220 230 240 VSQFETHPYF QRDSLVKFCM KHGVLPTAHT PLGGAAANKD MFGSVSPLDD PVLNDVAKKY 250 260 270 280 290 300 GKSVAQICLR WGIQRKTAVI PKSSKIQRLK ENLEVLEFQL SDEDMQLIYS IDRKYRTSLP 310 SKTWGLDVYA

References

[1]	"Nucleotide sequence of a cDNA encoding NADP-sorbitol-6-phosphate dehydrogenase from apple." Kanayama Y., Mori H., Imaseki H., Yamaki S. Plant Physiol. 100:1607-1608(1992) [PubMed: 16653170] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Genomic nucleotide sequence of NADP sorbitol-6-phosphate dehydrogenase (NADP-S6PDH) gene from apple." Bains H.S., Tao R., Uratsu S.L., Dandekar A.M. Plant Gene Register PGR98-193 Cited for: NUCLEOTIDE SEQUENCE. Strain: cv. Greensleeves.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D11080 mRNA. Translation: BAA01853.1. AF057134 Genomic DNA. Translation: AAC97607.1.
PIR	T17013.
3D structure databases
ProteinModelPortal	P28475.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-11700.
Family and domain databases
InterPro	IPR001395. Aldo/ket_red. IPR018170. Aldo/ket_reductase_CS. IPR020471. Aldo/keto_reductase_subgr. IPR023210. NADP_OxRdtase_dom. [Graphical view]
Gene3D	G3DSA:3.20.20.100. Aldo/ket_red. 1 hit.
PANTHER	PTHR11732. Aldo/ket_red. 1 hit.
Pfam	PF00248. Aldo_ket_red. 1 hit. [Graphical view]
PIRSF	PIRSF000097. AKR. 1 hit.
PRINTS	PR00069. ALDKETRDTASE.
SUPFAM	SSF51430. Aldo/ket_red. 1 hit.
PROSITE	PS00798. ALDOKETO_REDUCTASE_1. 1 hit. PS00062. ALDOKETO_REDUCTASE_2. 1 hit. PS00063. ALDOKETO_REDUCTASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

S6PD_MALDO

Accession

Primary (citable) accession number: P28475

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	October 19, 2011
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program