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Protein

Gamma-aminobutyric acid receptor subunit alpha-4

Gene

Gabra4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.

GO - Molecular functioni

  1. chloride channel activity Source: UniProtKB-KW
  2. extracellular ligand-gated ion channel activity Source: InterPro
  3. GABA-A receptor activity Source: RGD

GO - Biological processi

  1. central nervous system development Source: Ensembl
  2. gamma-aminobutyric acid signaling pathway Source: InterPro
  3. regulation of response to drug Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Chloride

Enzyme and pathway databases

ReactomeiREACT_199021. GABA A receptor activation.
REACT_199029. Ligand-gated ion channel transport.
REACT_270861. Orphan transporters.

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-aminobutyric acid receptor subunit alpha-4
Alternative name(s):
GABA(A) receptor subunit alpha-4
Gene namesi
Name:Gabra4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 14

Organism-specific databases

RGDi621670. Gabra4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini36 – 258223ExtracellularCuratedAdd
BLAST
Transmembranei259 – 28022HelicalCuratedAdd
BLAST
Transmembranei285 – 30622HelicalCuratedAdd
BLAST
Transmembranei318 – 34023HelicalCuratedAdd
BLAST
Topological domaini341 – 521181CytoplasmicCuratedAdd
BLAST
Transmembranei522 – 54120HelicalCuratedAdd
BLAST

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. chloride channel complex Source: UniProtKB-KW
  3. postsynaptic membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3535Sequence AnalysisAdd
BLAST
Chaini36 – 552517Gamma-aminobutyric acid receptor subunit alpha-4PRO_0000000443Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi47 – 471N-linked (GlcNAc...)Sequence Analysis
Glycosylationi144 – 1441N-linked (GlcNAc...)Sequence Analysis
Glycosylationi157 – 1571N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi172 ↔ 186By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP28471.
PRIDEiP28471.

PTM databases

PhosphoSiteiP28471.

Expressioni

Gene expression databases

GenevestigatoriP28471.

Interactioni

Subunit structurei

Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003191.

Structurei

3D structure databases

ProteinModelPortaliP28471.
SMRiP28471. Positions 285-344.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG238757.
GeneTreeiENSGT00760000119010.
HOVERGENiHBG051707.
InParanoidiP28471.
KOiK05175.
OMAiSASTHRV.
OrthoDBiEOG7JX342.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR001390. GABAAa_rcpt.
IPR005434. GABBAa4_rcpt.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR01079. GABAARALPHA.
PR01617. GABAARALPHA4.
PR00253. GABAARECEPTR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 2 hits.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28471-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSVQKVPAI VLCSGVSLAL LHVLCLATCL NESPGQNSKD EKLCPENFTR
60 70 80 90 100
ILDSLLDGYD NRLRPGFGGP VTEVKTDIYV TSFGPVSDVE MEYTMDVFFR
110 120 130 140 150
QTWIDKRLKY DGPIEILRLN NMMVTKVWTP DTFFRNGKKS VSHNMTAPNK
160 170 180 190 200
LFRIMRNGTI LYTMRLTISA ECPMRLVDFP MDGHACPLKF GSYAYPKSEM
210 220 230 240 250
IYTWTKGPEK SVEVPKESSS LVQYDLIGQT VSSETIKSIT GEYIVMTVYF
260 270 280 290 300
HLRRKMGYFM IQTYIPCIMT VILSQVSFWI NKESVPARTV FGITTVLTMT
310 320 330 340 350
TLSISARHSL PKVSYATAMD WFIAVCFAFV FSALIEFAAV NYFTNIQMQK
360 370 380 390 400
AKKKISKPPP EVPAAPVLKE KHTETSLQNT HANLNMRKRT NALVHSESDV
410 420 430 440 450
NSRTEVGNHS SKTTAAQESS ETTPKAHLAS SPNPFSRANA AETISAAARG
460 470 480 490 500
LSSAASPSPH GTLQPAPLRS ASARPAFGAR LGRIKTTVNT TGVPGNVSAT
510 520 530 540 550
PPPSAPPPSG SGTSKIDKYA RILFPVTFGA FNMVYWVVYL SKDTMEKSES

LM
Length:552
Mass (Da):60,951
Last modified:December 1, 1992 - v1
Checksum:i3823335C3554115D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08493 Genomic DNA. Translation: AAC42032.1.
S55933 mRNA. Translation: AAB19902.1.
PIRiS17551.
RefSeqiNP_542154.3. NM_080587.3.
UniGeneiRn.81205.

Genome annotation databases

EnsembliENSRNOT00000003191; ENSRNOP00000003191; ENSRNOG00000002336.
GeneIDi140675.
KEGGirno:140675.
UCSCiRGD:621670. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08493 Genomic DNA. Translation: AAC42032.1.
S55933 mRNA. Translation: AAB19902.1.
PIRiS17551.
RefSeqiNP_542154.3. NM_080587.3.
UniGeneiRn.81205.

3D structure databases

ProteinModelPortaliP28471.
SMRiP28471. Positions 285-344.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003191.

Chemistry

BindingDBiP28471.
ChEMBLiCHEMBL2096683.
GuidetoPHARMACOLOGYi407.

PTM databases

PhosphoSiteiP28471.

Proteomic databases

PaxDbiP28471.
PRIDEiP28471.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000003191; ENSRNOP00000003191; ENSRNOG00000002336.
GeneIDi140675.
KEGGirno:140675.
UCSCiRGD:621670. rat.

Organism-specific databases

CTDi2557.
RGDi621670. Gabra4.

Phylogenomic databases

eggNOGiNOG238757.
GeneTreeiENSGT00760000119010.
HOVERGENiHBG051707.
InParanoidiP28471.
KOiK05175.
OMAiSASTHRV.
OrthoDBiEOG7JX342.

Enzyme and pathway databases

ReactomeiREACT_199021. GABA A receptor activation.
REACT_199029. Ligand-gated ion channel transport.
REACT_270861. Orphan transporters.

Miscellaneous databases

NextBioi620671.

Gene expression databases

GenevestigatoriP28471.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR001390. GABAAa_rcpt.
IPR005434. GABBAa4_rcpt.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR01079. GABAARALPHA.
PR01617. GABAARALPHA4.
PR00253. GABAARECEPTR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 2 hits.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning, pharmacological characteristics and expression pattern of the rat GABAA receptor alpha 4 subunit."
    Wisden W., Herb A., Wieland H., Keinaenen K., Lueddens H., Seeburg P.H.
    FEBS Lett. 289:227-230(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiGBRA4_RAT
AccessioniPrimary (citable) accession number: P28471
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: January 7, 2015
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.