Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Isocitrate lyase

Gene

aceA

Organism
Acinetobacter calcoaceticus
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the metabolic adaptation in response to environmental changes. Catalyzes the reversible formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle, which operates as an anaplerotic route for replenishing the tricarboxylic acid cycle during growth on fatty acid substrates.1 Publication

Catalytic activityi

Isocitrate = succinate + glyoxylate.1 Publication

Cofactori

Mg2+1 PublicationNote: Divalent metal cations. Mn(2+), Fe(2+) or Co2+ can be used.1 Publication

Enzyme regulationi

Inhibited by hydroxymalonate, oxalate and itaconate.1 Publication

Kineticsi

  1. KM=40 µM for threo-D-isocitrate.1 Publication

pH dependencei

Optimum pH is 7.5.1 Publication

Pathwayi

GO - Molecular functioni

  1. isocitrate lyase activity Source: UniProtKB-EC

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-UniPathway
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Cobalt, Iron, Magnesium, Manganese

Enzyme and pathway databases

UniPathwayiUPA00703; UER00719.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate lyase1 Publication (EC:4.1.3.11 Publication)
Short name:
ICL1 Publication
Alternative name(s):
Isocitrase1 Publication
Isocitratase1 Publication
Gene namesi
Name:aceA
OrganismiAcinetobacter calcoaceticus
Taxonomic identifieri471 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacterAcinetobacter calcoaceticus/baumannii complex

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – ›15›15Isocitrate lyasePRO_0000068770Add
BLAST

Interactioni

Subunit structurei

Homotetramer.1 Publication

Family & Domainsi

Sequence similaritiesi

Sequencei

Sequence statusi: Fragment.

Length:15
Mass (Da):1,710
Last modified:December 1, 1992 - v1
Checksum:i83AE726B1F2F96E3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei15 – 151

Sequence databases

PIRiA41338.

Cross-referencesi

Sequence databases

PIRiA41338.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00703; UER00719.

Family and domain databases

ProtoNetiSearch...

Publicationsi

  1. "Purification and characterization of Acinetobacter calcoaceticus isocitrate lyase."
    Hoyt J.C., Johnson K.E., Reeves H.C.
    J. Bacteriol. 173:6844-6848(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, COFACTOR, SUBUNIT.
    Strain: B4.

Entry informationi

Entry nameiACEA_ACICA
AccessioniPrimary (citable) accession number: P28467
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: April 29, 2015
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.