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Isocitrate lyase



Acinetobacter calcoaceticus
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli


Involved in the metabolic adaptation in response to environmental changes. Catalyzes the reversible formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle, which operates as an anaplerotic route for replenishing the tricarboxylic acid cycle during growth on fatty acid substrates.1 Publication

Catalytic activityi

Isocitrate = succinate + glyoxylate.1 Publication


Mg2+1 PublicationNote: Divalent metal cations. Mn2+, Fe2+ or Co2+ can be used.1 Publication

Enzyme regulationi

Inhibited by hydroxymalonate, oxalate and itaconate.1 Publication


  1. KM=40 µM for threo-D-isocitrate1 Publication

    pH dependencei

    Optimum pH is 7.5.1 Publication

    Pathwayi: glyoxylate cycle

    This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from isocitrate.1 Publication
    Proteins known to be involved in the 2 steps of the subpathway in this organism are:
    1. Isocitrate lyase (aceA)
    2. Malate synthase G (glcB), Malate synthase G (glcB), Malate synthase G (glcB), Malate synthase G (glcB)
    This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

    GO - Molecular functioni

    GO - Biological processi


    Molecular functionLyase
    Biological processGlyoxylate bypass, Tricarboxylic acid cycle
    LigandCobalt, Iron, Magnesium, Manganese

    Enzyme and pathway databases

    UniPathwayiUPA00703; UER00719.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isocitrate lyase1 Publication (EC: Publication)
    Short name:
    ICL1 Publication
    Alternative name(s):
    Isocitrase1 Publication
    Isocitratase1 Publication
    Gene namesi
    OrganismiAcinetobacter calcoaceticus
    Taxonomic identifieri471 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacterAcinetobacter calcoaceticus/baumannii complex

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000687701 – ›15Isocitrate lyaseAdd BLAST›15


    Subunit structurei

    Homotetramer.1 Publication

    Family & Domainsi

    Sequence similaritiesi


    Sequence statusi: Fragment.

    Mass (Da):1,710
    Last modified:December 1, 1992 - v1

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Non-terminal residuei151

    Sequence databases


    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

    Entry informationi

    Entry nameiACEA_ACICA
    AccessioniPrimary (citable) accession number: P28467
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: December 1, 1992
    Last modified: March 15, 2017
    This is version 58 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program


    Keywords - Technical termi

    Direct protein sequencing


    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families