P28461 (IF5A_SULAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Translation initiation factor 5A

Alternative name(s):
Hypusine-containing protein
SHP
eIF-5A

Gene names

Name:	eif5a
Ordered Locus Names:	Saci_1311

Organism

Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) [Complete proteome] [HAMAP]

Taxonomic identifier

330779 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	131 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions by promoting the formation of the first peptide bond By similarity. HAMAP MF_00085
Subcellular location	Cytoplasm HAMAP MF_00085.
Post-translational modification	The N-terminus is blocked. HAMAP MF_00085
Sequence similarities	Belongs to the eIF-5A family.
Sequence caution	The sequence CAA44842.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Molecular function	Initiation factor
PTM	Hypusine
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	peptidyl-lysine modification to hypusine Inferred from electronic annotation. Source: InterPro positive regulation of translational elongation Inferred from electronic annotation. Source: InterPro positive regulation of translational termination Inferred from electronic annotation. Source: InterPro translational frameshifting Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ribosome binding Inferred from electronic annotation. Source: InterPro translation elongation factor activity Inferred from electronic annotation. Source: InterPro translation initiation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 131	131	Translation initiation factor 5A HAMAP MF_00085		PRO_0000142503
Amino acid modifications
	Modified residue	36	1	Hypusine HAMAP MF_00085
Experimental info
	Sequence conflict	129	1	R → Y AA sequence Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P28461 [UniParc]. Last modified March 27, 2002. Version 2. Checksum: 3857AE86925DDC11	FASTA	131	14,535
	10 20 30 40 50 60 MSIQYTTVGD LKVGSYVMID GEPCRVVEIT KAKTGKHGSA KANVVAIGLF TGQKRSLMAP 70 80 90 100 110 120 VDQQVEVPII EKHVGQILAD KGDNLTIMDL ESYETFDLEK PTENEIVSKI RPGAEIEYWS 130 VMGRRKIVRV K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The archaebacterial hypusine-containing protein. Structural features suggest common ancestry with eukaryotic translation initiation factor 5A." Bartig D., Lemkemeier K., Frank J., Lottspeich F., Klink F. Eur. J. Biochem. 204:751-758(1992) [PubMed: 1541288] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, HYPUSINE AT LYS-36. Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.
[2]	"The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota." Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E., Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A. J. Bacteriol. 187:4992-4999(2005) [PubMed: 15995215] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X63132 Genomic DNA. Translation: CAA44842.1. Different initiation. CP000077 Genomic DNA. Translation: AAY80649.1.
PIR	S22380.
RefSeq	YP_255942.1. NC_007181.1.
3D structure databases
ProteinModelPortal	P28461.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3473388.
GenomeReviews	Gene locus Saci_1311 in contig CP000077_GR.
KEGG	sai:Saci_1311.
NMPDR	fig|330779.3.peg.121.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG497107.
OMA	VEISKAK.
PhylomeDB	P28461.
ProtClustDB	PRK03999.
Enzyme and pathway databases
BioCyc	SACI330779:SACI_1311-MONOMER.
Family and domain databases
HAMAP	MF_00085. eIF-5A. [Tree]
InterPro	IPR005824. KOW. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR019769. Trans_elong_IF5A_hypusine_site. IPR001884. Transl_elong_IF5A. IPR022847. Transl_elong_IF5A_arc. IPR020189. Transl_elong_IF5A_C. IPR014722. Transl_SH3-like_sub. IPR008991. Translation_prot_SH3-like. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:2.30.30.30. Ribosomal_L2. 1 hit.
KO	K03263.
PANTHER	PTHR11673. EIF5A_hypusine. 1 hit. PTHR11673:SF1. PTHR11673:SF1. 1 hit.
Pfam	PF01287. eIF-5a. 1 hit. PF00467. KOW. 1 hit. [Graphical view]
PIRSF	PIRSF003025. eIF5A. 1 hit.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF50104. Transl_SH3_like. 1 hit.
TIGRFAMs	TIGR00037. EIF_5A. 1 hit.
PROSITE	PS00302. IF5A_HYPUSINE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IF5A_SULAC

Accession

Primary (citable) accession number: P28461
Secondary accession number(s): Q4J981

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	March 27, 2002
Last modified:	December 14, 2011
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program