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Protein

Light-independent protochlorophyllide reductase subunit N

Gene

chlN

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Component of the dark-operative protochlorophyllide reductase (DPOR) that uses Mg-ATP and reduced ferredoxin to reduce ring D of protochlorophyllide (Pchlide) to form chlorophyllide a (Chlide). This reaction is light-independent. The NB-protein (ChlN-ChlB) is the catalytic component of the complex.UniRule annotation

Catalytic activityi

Protochlorophyllide a + reduced ferredoxin + 2 ATP + 2 H2O = chlorophyllide a + oxidized ferredoxin + 2 ADP + 2 phosphate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per heterodimer. The cluster is bound at the heterodimer interface by residues from both subunits.UniRule annotation

Pathway:ichlorophyll biosynthesis (light-independent)

This protein is involved in the pathway chlorophyll biosynthesis (light-independent), which is part of Porphyrin-containing compound metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway chlorophyll biosynthesis (light-independent) and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi24 – 241Iron-sulfur (4Fe-4S); shared with heterodimeric partnerUniRule annotation
Metal bindingi49 – 491Iron-sulfur (4Fe-4S); shared with heterodimeric partnerUniRule annotation
Metal bindingi109 – 1091Iron-sulfur (4Fe-4S); shared with heterodimeric partnerUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Chlorophyll biosynthesis, Photosynthesis

Keywords - Ligandi

4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00670.

Names & Taxonomyi

Protein namesi
Recommended name:
Light-independent protochlorophyllide reductase subunit NUniRule annotation (EC:1.3.7.7UniRule annotation)
Short name:
DPOR subunit NUniRule annotation
Short name:
LI-POR subunit NUniRule annotation
Gene namesi
Name:chlNUniRule annotation
Ordered Locus Names:slr0750
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
ProteomesiUP000001425 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 469469Light-independent protochlorophyllide reductase subunit NPRO_0000208605Add
BLAST

Interactioni

Subunit structurei

Protochlorophyllide reductase is composed of three subunits; ChlL, ChlN and ChlB. Forms a heterotetramer of two ChlB and two ChlN subunits.UniRule annotation

Protein-protein interaction databases

IntActiP28372. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliP28372.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the BchN/ChlN family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2710.
HOGENOMiHOG000265994.
InParanoidiP28372.
KOiK04038.
OMAiIFAEPRF.
OrthoDBiEOG6PZX7T.
PhylomeDBiP28372.

Family and domain databases

HAMAPiMF_00352. ChlN_BchN.
InterProiIPR000510. Nase/OxRdtase_comp1.
IPR005970. Protochl_reductN.
[Graphical view]
PfamiPF00148. Oxidored_nitro. 1 hit.
[Graphical view]
PIRSFiPIRSF000162. P_chlorophyll_rd. 1 hit.
TIGRFAMsiTIGR01279. DPOR_bchN. 1 hit.

Sequencei

Sequence statusi: Complete.

P28372-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVAQQAPSA LNFDCETGNY HTFCPISCVS WLYQKIEDSF FLVIGTKTCG
60 70 80 90 100
YFLQNAMGVM IFAEPRYAMA ELEEGDISAQ LKDYEELKRL CLQIKRDRNP
110 120 130 140 150
SVIVWIGTCT TEIIKMDLEG LAPQLEAEIG IPIVTARANG LDYAFTQGED
160 170 180 190 200
TVLASMAHKC PTSAQVQGED KEERNAIQKL LTFGRKADQE KVESEYVDHQ
210 220 230 240 250
PLVLFGSLPD PVVTNLTLEL KKQGVKVSGW LPAKRYTELP VIDEGYYVAG
260 270 280 290 300
VNPFLSRTAT TLMRRRKCKL IGAPFPIGPD GTRAWIEKIC SVLGIEPQGL
310 320 330 340 350
EEREAQIWAS LEDYIQLIRG KSVFFMGDNL LEVSLARFLI RCGMTCPEIG
360 370 380 390 400
IPYMDKRYQA AELALLEKTC SDMGVPLPNI VEKPDNYNQI QRIKALQPDL
410 420 430 440 450
VITGMAHANP LEAQGINTKW SVEFTFAQIH GFTNARDILE LATRPLRRNS
460
QLGELGWDKL IAKDVPAQV
Length:469
Mass (Da):52,475
Last modified:December 1, 1992 - v1
Checksum:i0A1BC3A7C4BBD84A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10474 Genomic DNA. Translation: BAA01276.1.
BA000022 Genomic DNA. Translation: BAA18746.1.
PIRiJT0601.

Genome annotation databases

EnsemblBacteriaiBAA18746; BAA18746; BAA18746.
KEGGisyy:SYNGTS_3040.
syz:MYO_130760.
PATRICi23843678. VBISynSp132158_3369.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10474 Genomic DNA. Translation: BAA01276.1.
BA000022 Genomic DNA. Translation: BAA18746.1.
PIRiJT0601.

3D structure databases

ProteinModelPortaliP28372.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP28372. 2 interactions.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA18746; BAA18746; BAA18746.
KEGGisyy:SYNGTS_3040.
syz:MYO_130760.
PATRICi23843678. VBISynSp132158_3369.

Phylogenomic databases

eggNOGiCOG2710.
HOGENOMiHOG000265994.
InParanoidiP28372.
KOiK04038.
OMAiIFAEPRF.
OrthoDBiEOG6PZX7T.
PhylomeDBiP28372.

Enzyme and pathway databases

UniPathwayiUPA00670.

Family and domain databases

HAMAPiMF_00352. ChlN_BchN.
InterProiIPR000510. Nase/OxRdtase_comp1.
IPR005970. Protochl_reductN.
[Graphical view]
PfamiPF00148. Oxidored_nitro. 1 hit.
[Graphical view]
PIRSFiPIRSF000162. P_chlorophyll_rd. 1 hit.
TIGRFAMsiTIGR01279. DPOR_bchN. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and nucleotide sequence of a frxC-ORF469 gene cluster of Synechocystis PCC6803: conservation with liverwort chloroplast frxC-ORF465 and nif operon."
    Ogura Y., Takemura M., Oda K., Yamato K., Ohta E., Fukuzawa H., Ohyama K.
    Biosci. Biotechnol. Biochem. 56:788-793(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.

Entry informationi

Entry nameiCHLN_SYNY3
AccessioniPrimary (citable) accession number: P28372
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: July 22, 2015
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.