Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P28372 (CHLN_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Light-independent protochlorophyllide reductase subunit N

Short name=DPOR subunit N
Short name=LI-POR subunit N
EC=1.3.7.7
Gene names
Name:chlN
Ordered Locus Names:slr0750
OrganismSynechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]
Taxonomic identifier1111708 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis

Protein attributes

Sequence length469 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Component of the dark-operative protochlorophyllide reductase (DPOR) that uses Mg-ATP and reduced ferredoxin to reduce ring D of protochlorophyllide (Pchlide) to form chlorophyllide a (Chlide). This reaction is light-independent. The NB-protein (ChlN-ChlB) is the catalytic component of the complex By similarity. HAMAP-Rule MF_00352

Catalytic activity

Protochlorophyllide a + reduced ferredoxin + 2 ATP + 2 H2O = chlorophyllide a + oxidized ferredoxin + 2 ADP + 2 phosphate. HAMAP-Rule MF_00352

Cofactor

Binds 1 [4Fe-4S] cluster per heterodimer. The cluster is bound at the heterodimer interface by residues from both subunits By similarity.

Pathway

Porphyrin-containing compound metabolism; chlorophyll biosynthesis (light-independent). HAMAP-Rule MF_00352

Subunit structure

Protochlorophyllide reductase is composed of three subunits; ChlL, ChlN and ChlB. Forms a heterotetramer of two ChlB and two ChlN subunits By similarity.

Sequence similarities

Belongs to the BchN/ChlN family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 469469Light-independent protochlorophyllide reductase subunit N HAMAP-Rule MF_00352
PRO_0000208605

Sites

Metal binding241Iron-sulfur (4Fe-4S); shared with heterodimeric partner By similarity
Metal binding491Iron-sulfur (4Fe-4S); shared with heterodimeric partner By similarity
Metal binding1091Iron-sulfur (4Fe-4S); shared with heterodimeric partner By similarity

Sequences

Sequence LengthMass (Da)Tools
P28372 [UniParc].

Last modified December 1, 1992. Version 1.
Checksum: 0A1BC3A7C4BBD84A

FASTA46952,475
        10         20         30         40         50         60 
MTVAQQAPSA LNFDCETGNY HTFCPISCVS WLYQKIEDSF FLVIGTKTCG YFLQNAMGVM 

        70         80         90        100        110        120 
IFAEPRYAMA ELEEGDISAQ LKDYEELKRL CLQIKRDRNP SVIVWIGTCT TEIIKMDLEG 

       130        140        150        160        170        180 
LAPQLEAEIG IPIVTARANG LDYAFTQGED TVLASMAHKC PTSAQVQGED KEERNAIQKL 

       190        200        210        220        230        240 
LTFGRKADQE KVESEYVDHQ PLVLFGSLPD PVVTNLTLEL KKQGVKVSGW LPAKRYTELP 

       250        260        270        280        290        300 
VIDEGYYVAG VNPFLSRTAT TLMRRRKCKL IGAPFPIGPD GTRAWIEKIC SVLGIEPQGL 

       310        320        330        340        350        360 
EEREAQIWAS LEDYIQLIRG KSVFFMGDNL LEVSLARFLI RCGMTCPEIG IPYMDKRYQA 

       370        380        390        400        410        420 
AELALLEKTC SDMGVPLPNI VEKPDNYNQI QRIKALQPDL VITGMAHANP LEAQGINTKW 

       430        440        450        460 
SVEFTFAQIH GFTNARDILE LATRPLRRNS QLGELGWDKL IAKDVPAQV 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and nucleotide sequence of a frxC-ORF469 gene cluster of Synechocystis PCC6803: conservation with liverwort chloroplast frxC-ORF465 and nif operon."
Ogura Y., Takemura M., Oda K., Yamato K., Ohta E., Fukuzawa H., Ohyama K.
Biosci. Biotechnol. Biochem. 56:788-793(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. expand/collapse author list , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D10474 Genomic DNA. Translation: BAA01276.1.
BA000022 Genomic DNA. Translation: BAA18746.1.
PIRJT0601.
RefSeqNP_442934.1. NC_000911.1.
YP_005652993.1. NC_017277.1.
YP_007452809.1. NC_020286.1.

3D structure databases

ProteinModelPortalP28372.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP28372. 2 interactions.
STRING1148.slr0750.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAA18746; BAA18746; BAA18746.
GeneID12255444.
14618501.
951977.
KEGGsyn:slr0750.
syy:SYNGTS_3040.
syz:MYO_130760.
PATRIC23843678. VBISynSp132158_3369.

Phylogenomic databases

eggNOGCOG2710.
HOGENOMHOG000265994.
KOK04038.
OMAIFAEPRF.
OrthoDBEOG6PZX7T.
PhylomeDBP28372.
ProtClustDBPRK02842.

Enzyme and pathway databases

UniPathwayUPA00670.

Family and domain databases

HAMAPMF_00352. ChlN_BchN.
InterProIPR000510. Nase/OxRdtase_comp1.
IPR005970. Protochl_reductN.
[Graphical view]
PfamPF00148. Oxidored_nitro. 1 hit.
[Graphical view]
PIRSFPIRSF000162. P_chlorophyll_rd. 1 hit.
TIGRFAMsTIGR01279. DPOR_bchN. 1 hit.
ProtoNetSearch...

Entry information

Entry nameCHLN_SYNY3
AccessionPrimary (citable) accession number: P28372
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: April 16, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Synechocystis PCC 6803

Synechocystis (strain PCC 6803): entries and gene names

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways