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Reviewed, UniProtKB/Swiss-Prot P28371 (EFG1_SYNY3)

Last modified June 16, 2009. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Elongation factor G 1
      Short name=EF-G 1
Gene names
Name: fusA
Synonyms: fus
Ordered Locus Names: slr1463
OrganismSynechocystis sp. (strain PCC 6803) [Complete proteome] [HAMAP]
Taxonomic identifier1148 [NCBI]
Taxonomic lineageBacteria › Cyanobacteria › Chroococcales › Synechocystis

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Protein attributes

Sequence length695 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

This protein promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. HAMAP MF_00054

Subcellular location

Cytoplasm. HAMAP MF_00054

Sequence similarities

Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.

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Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandGTP-binding
Nucleotide-binding
   Molecular functionElongation factor
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processtranslational elongation

Inferred from electronic annotation. Source: HAMAP

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionGTP binding

Inferred from electronic annotation. Source: HAMAP

GTPase activity

Inferred from electronic annotation. Source: InterPro

protein binding

Inferred from physical interaction. Source: IntAct

translation elongation factor activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

trxAP522311EBI-862177,EBI-862916

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 695695Elongation factor G 1 HAMAP MF_00054
PRO_0000091243

Regions

Nucleotide binding15 – 228GTP By similarity
Nucleotide binding79 – 835GTP By similarity
Nucleotide binding133 – 1364GTP By similarity

Experimental info

Sequence conflict421H → R in CAA46277. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
P28371-1 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: 8727830BF9073223

FASTA69576,750
        10         20         30         40         50         60 
MEKDLTRYRN IGIFAHVDAG KTTTTERILK LTGRIHKLGE VHEGESTMDF MEQEAERGIT 

        70         80         90        100        110        120 
IQSAATSCFW KDHQLNVIDT PGHVDFTIEV YRSLKVLDGG IGVFCGSGGV EPQSETNWRY 

       130        140        150        160        170        180 
ANDSKVARLI YINKLDRTGA DFYRVVKQVE TVLGAKPLVM TLPIGTENDF VGVVDILTEK 

       190        200        210        220        230        240 
AYIWDDSGDP EKYEITDIPA DMVDDVATYR EMLIETAVEQ DDDLMEKYLE GEEISIDDIK 

       250        260        270        280        290        300 
RCIRTGTRKL DFFPTYGGSS FKNKGVQLVL DAVVDYLPNP KEVPPQPEVD LEGEETGNYA 

       310        320        330        340        350        360 
IVDPEAPLRA LAFKIMDDRF GALTFTRIYS GTLSKGDTIL NTATGKTERI GRLVEMHADS 

       370        380        390        400        410        420 
REEIESAQAG DIVAIVGMKN VQTGHTLCDP KNPATLEPMV FPDPVISIAI KPKKKGMDEK 

       430        440        450        460        470        480 
LGMALSKMVQ EDPSFQVETD EESGETIIKG MGELHLDIKM DILKRTHGVE VEMGKPQVAY 

       490        500        510        520        530        540 
RESITQQVSD TYVHKKQSGG SGQYAKIDYI VEPGEPGSGF QFESKVTGGN VPREYWPAVQ 

       550        560        570        580        590        600 
KGFDQSVVKG VLAGYPVVDL KVTLTDGGFH PVDSSAIAFE IAAKAGYRQS LPKAKPQILE 

       610        620        630        640        650        660 
PIMAVDVFTP EDHMGDVIGD LNRRRGMIKS QETGPMGVRV KADVPLSEMF GYIGDLRTMT 

       670        680        690 
SGRGQFSMVF DHYAPCPTNV AEEVIKEAKE RQAAA

« Hide

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References

« Hide 'large scale' references
[1]"Synechocystis sp. PCC6803 fusB gene, located outside of the str operon, encodes a polypeptide related to protein synthesis factor EF-G."
Welsch P.L., Johnson D., Zhang Y., Breitenberger C.A.
Plant Mol. Biol. 25:735-738(1994) [PubMed: 8061323] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. expand/collapse author list , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases

X65159 Genomic DNA. Translation: CAA46277.1.
BA000022 Genomic DNA. Translation: BAA18663.1.
PIRS76751.
RefSeqNP_442851.1.

3D structure databases

HSSPHSSP built from PDB template 2EFG based on UniProtKB P13551.
ModBaseSearch...

Protein-protein interaction databases

IntActP28371. 3 interactions.

Genome annotation databases

GeneID953171.
GenomeReviewsGene locus slr1463 in contig BA000022_GR.
KEGGsyn:slr1463.
NMPDRfig|1148.1.peg.2952.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMP28371.
OMAP28371. TTDFMEQ.

Enzyme and pathway databases

BioCycSSP1148:SLR1463-MON.

Family and domain databases

HAMAPMF_00054.
[Tree]
InterProIPR000795. ProtSyn_GTP_bd.
IPR014721. Ribosomal_S5_D2-type_fold.
IPR005225. Small_GTP_bd.
IPR004540. Transl_elong_EFG/EF2.
IPR000640. Transl_elong_EFG/EF2_C.
IPR005517. Transl_elong_EFG/EF2_IV.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
Gene3DG3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
G3DSA:3.30.70.240. Transl_elong_EFG/EF2_C. 1 hit.
PfamPF00679. EFG_C. 1 hit.
PF03764. EFG_IV. 1 hit.
PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSPR00315. ELONGATNFCT.
TIGRFAMsTIGR00484. EF-G. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEPS00301. EFACTOR_GTP. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameEFG1_SYNY3
AccessionPrimary (citable) accession number: P28371
Secondary accession number(s): P74556
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: November 1, 1997
Last modified: June 16, 2009
This is version 67 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Synechocystis PCC 6803

Synechocystis (strain PCC 6803): entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents