Reviewed, UniProtKB/Swiss-Prot P28369 (RFH_ECOLI)

Names and origin

Protein names

Recommended name:
    Putative peptide chain release factor homolog
      Short name=RF-H

Gene names

Name:	prfH
Ordered Locus Names:	b0236, JW0226

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	166 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Uncertain.

General annotation (Comments)

Function	Could be involved in translational termination.
Sequence similarities	Belongs to the prokaryotic/mitochondrial release factor family.
Caution	Could be the product of a pseudogene.
Sequence caution	The sequence M34034 differs from that shown. Reason: Frameshift at position 32.

Ontologies

Keywords
Biological process	Protein biosynthesis
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	translational termination Inferred from electronic annotation. Source: InterPro
Molecular function	protein binding Inferred from physical interaction. Source: IntAct translation release factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
yccT	P0A8X4	1	EBI-1121159,EBI-1121145

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 166	166	Putative peptide chain release factor homolog		PRO_0000166857
Regions
	Compositional bias	129 – 132	4	Poly-Gln

Sequences

	Sequence		Length	Mass (Da)
	P28369-1 [UniParc]. Last modified September 19, 2006. Version 2. Checksum: 7555352297B51280	FASTA	166	18,834
	10 20 30 40 50 60 MLETETGRYS DTLRSALVSL DGDNAWALSE SWCGTIQWIC PSPYRPHHGR KNWFLGIGRF 70 80 90 100 110 120 TADEQEQSDA IRYETLRSSG PGGQHVNKTD SAVRATHLAS GISVKVQSER SQHANKRLAR 130 140 150 160 LLIAWKLEQQ QQENSAALKS QRRMFHHQIE RGNPRRTFTG MAFIEG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Peptidase D gene (pepD) of Escherichia coli K-12: nucleotide sequence, transcript mapping, and comparison with other peptidase genes." Henrich B., Monnerjahn U., Plapp R. J. Bacteriol. 172:4641-4651(1990) [PubMed: 1695895] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Systematic sequencing of the Escherichia coli genome: analysis of the 4.0 - 6.0 min (189,987 - 281,416bp) region." Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T., Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S., Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K. Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO N-TERMINUS. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Sequence comparison of new prokaryotic and mitochondrial members of the polypeptide chain release factor family predicts a five-domain model for release factor structure." Pel H.J., Rep M., Grivell L.A. Nucleic Acids Res. 20:4423-4428(1992) [PubMed: 1408743] [Abstract] Cited for: IDENTIFICATION OF PROTEIN.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M34034 Unassigned DNA. No translation available. U70214 Genomic DNA. Translation: AAB08656.1. U00096 Genomic DNA. No translation available. AP009048 Genomic DNA. Translation: BAA77905.2.
PIR	E64748.
RefSeq	AP_000889.1.
3D structure databases
HSSP	HSSP built from PDB template 1GQE based on UniProtKB P07012.
ModBase	Search...
Protein-protein interaction databases
IntAct	P28369. 1 interaction.
Genome annotation databases
GenomeReviews	Gene locus JW0226 in contig AP009048_GR. Gene locus b0236 in contig U00096_GR.
KEGG	ecj:JW0226. eco:b0236.
Organism-specific databases
EchoBASE	EB1459.
EcoGene	EG11496. prfH.
CMR	Search...
Phylogenomic databases
HOGENOM	P28369.
OMA	P28369. RKNWFIG.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11496-MON.
Family and domain databases
InterPro	IPR000352. Pep_rel_factor_I. IPR017509. Release_fac_PrfH. [Graphical view]
Pfam	PF00472. RF-1. 1 hit. [Graphical view]
TIGRFAMs	TIGR03072. release_prfH. 1 hit.
PROSITE	PS00745. RF_PROK_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RFH_ECOLI

Accession

Primary (citable) accession number: P28369
Secondary accession number(s): P77246

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	September 19, 2006
Last modified:	June 16, 2009
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)