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Reviewed, UniProtKB/Swiss-Prot P28369 (RFH_ECOLI)

Last modified June 16, 2009. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Putative peptide chain release factor homolog
      Short name=RF-H
Gene names
Name: prfH
Ordered Locus Names: b0236, JW0226
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length166 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceUncertain.

General annotation (Comments)

Function

Could be involved in translational termination.

Sequence similarities

Belongs to the prokaryotic/mitochondrial release factor family.

Caution

Could be the product of a pseudogene.

Sequence caution

The sequence M34034 differs from that shown. Reason: Frameshift at position 32.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processtranslational termination

Inferred from electronic annotation. Source: InterPro

   Molecular functionprotein binding

Inferred from physical interaction. Source: IntAct

translation release factor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

yccTP0A8X41EBI-1121159,EBI-1121145

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 166166Putative peptide chain release factor homolog
PRO_0000166857

Regions

Compositional bias129 – 1324Poly-Gln

Sequences

Sequence LengthMass (Da)Tools
P28369-1 [UniParc].

Last modified September 19, 2006. Version 2.
Checksum: 7555352297B51280

FASTA16618,834
        10         20         30         40         50         60 
MLETETGRYS DTLRSALVSL DGDNAWALSE SWCGTIQWIC PSPYRPHHGR KNWFLGIGRF 

        70         80         90        100        110        120 
TADEQEQSDA IRYETLRSSG PGGQHVNKTD SAVRATHLAS GISVKVQSER SQHANKRLAR 

       130        140        150        160 
LLIAWKLEQQ QQENSAALKS QRRMFHHQIE RGNPRRTFTG MAFIEG 

« Hide

References

« Hide 'large scale' references
[1]"Peptidase D gene (pepD) of Escherichia coli K-12: nucleotide sequence, transcript mapping, and comparison with other peptidase genes."
Henrich B., Monnerjahn U., Plapp R.
J. Bacteriol. 172:4641-4651(1990) [PubMed: 1695895] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"Systematic sequencing of the Escherichia coli genome: analysis of the 4.0 - 6.0 min (189,987 - 281,416bp) region."
Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T., Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S., Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K.
Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"Sequence of minutes 4-25 of Escherichia coli."
Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO N-TERMINUS.
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"Sequence comparison of new prokaryotic and mitochondrial members of the polypeptide chain release factor family predicts a five-domain model for release factor structure."
Pel H.J., Rep M., Grivell L.A.
Nucleic Acids Res. 20:4423-4428(1992) [PubMed: 1408743] [Abstract]
Cited for: IDENTIFICATION OF PROTEIN.

Cross-references

Sequence databases

M34034 Unassigned DNA. No translation available.
U70214 Genomic DNA. Translation: AAB08656.1.
U00096 Genomic DNA. No translation available.
AP009048 Genomic DNA. Translation: BAA77905.2.
PIRE64748.
RefSeqAP_000889.1.

3D structure databases

HSSPHSSP built from PDB template 1GQE based on UniProtKB P07012.
ModBaseSearch...

Protein-protein interaction databases

IntActP28369. 1 interaction.

Genome annotation databases

GenomeReviewsGene locus JW0226 in contig AP009048_GR.
Gene locus b0236 in contig U00096_GR.
KEGGecj:JW0226.
eco:b0236.

Organism-specific databases

EchoBASEEB1459.
EcoGeneEG11496. prfH.
CMRSearch...

Phylogenomic databases

HOGENOMP28369.
OMAP28369. RKNWFIG.

Enzyme and pathway databases

BioCycEcoCyc:EG11496-MON.

Family and domain databases

InterProIPR000352. Pep_rel_factor_I.
IPR017509. Release_fac_PrfH.
[Graphical view]
PfamPF00472. RF-1. 1 hit.
[Graphical view]
TIGRFAMsTIGR03072. release_prfH. 1 hit.
PROSITEPS00745. RF_PROK_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRFH_ECOLI
AccessionPrimary (citable) accession number: P28369
Secondary accession number(s): P77246
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: September 19, 2006
Last modified: June 16, 2009
This is version 66 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents