P28365 (RPA2_EUPOC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase I subunit RPA2
Short name=RNA polymerase I subunit 2
EC=2.7.7.6

Gene names

Name:

RPA2

Organism

Euplotes octocarinatus

Taxonomic identifier

5937 [NCBI]

Taxonomic lineage

Eukaryota › Alveolata › Ciliophora › Intramacronucleata › Spirotrichea › Hypotrichia › Euplotida › Euplotidae › Euplotes

Protein attributes

Sequence length	1166 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits By similarity.
Subcellular location	Nucleus › nucleolus By similarity.
Sequence similarities	Belongs to the RNA polymerase beta chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase Nucleus
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
Gene Ontology (GO)
Cellular component	nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1166	1166	DNA-directed RNA polymerase I subunit RPA2		PRO_0000048076
Regions
	Zinc finger	1081 – 1130	50	C4-type Potential

Sequences

	Sequence		Length	Mass (Da)
	P28365 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 7253965D6173F0C9	FASTA	1,166	132,714
	10 20 30 40 50 60 MKTNAKFDRK EISKIYKNIA RHHIDSFDFA MSTCLNRACE HMLPFDYIVP EESASCGFKK 70 80 90 100 110 120 LTLWYDSFEL GQPSLGEIDY DSHILYPSEC RQRKMTYTIP LFATIFKKFD DEMVDNFKVK 130 140 150 160 170 180 LGDIPTMGRK KFCNLKGLTK KELAKRGEDM LEFGGYFIVN GNEKVIRMLI VPKRNFPIAF 190 200 210 220 230 240 KRSKFLERGK DFTDYGVQMR CVRDDFTAQT ITLTYLSDGS VSLRLIYQKQ EFLIPIILIL 250 260 270 280 290 300 KALKNCTDRQ IYERIVKGNF NQRQISDRVE AILAVGKDLN IYDSDQSKAL IGSRFRIVLA 310 320 330 340 350 360 GITSETSDID AGDLFLSKHI CIHTDSYEAK FDTLILMIDK LYASVANEVE LDNLDSVAMQ 370 380 390 400 410 420 DVLLGGHLYL QILSEKLFDC LHINLRARLN KELKRHNFDP MKFRDVLTNQ KINCGIGLIG 430 440 450 460 470 480 KRMENFLATG NLISRTNLDL MQTSGFCIIG DKLNNIRFLS HFRSIHRGQY FAEQKTTSVR 490 500 510 520 530 540 KLLPESWGFI CPVHTPDGAP CGLLNHISMS CVPIGSEEKQ IDIDKFRNIL GELGMNSISS 550 560 570 580 590 600 DLCLNYHTGY YPVIFDGIHL GYVEKDIGES FVEGLRYLKC TQSQPDYAIP RTLEIAFIPF 610 620 630 640 650 660 SGYSRNLQWP GIFLASTPAR FTRPVKNLHY NCIEWISPLE QMNLSIACTD EDITPETTHQ 670 680 690 700 710 720 ELDPINILSI VASVGVFAEY NQSPRNMYQC QMAKQTMGTP YHNHQFRTDN KIYRLLFPHR 730 740 750 760 770 780 PIVKTRTQVD FDIEEYPSGT NAVVAVISYT GYDLEDAMII NKSSYERGFG HGVVYKSYTH 790 800 810 820 830 840 DLNESNSQST RGIKSSVRYK FLNNVSQKDK SKIKLENIDP DGLPKIGSQL TKGKPELCIF 850 860 870 880 890 900 DTLKRGAKLS KFKDSEKARI ETVRVCGNDD KNPDNLSIGY TIRYSRIPVI GDKFSSRHGQ 910 920 930 940 950 960 KGVLSVLWPQ VDMPFTENGI TPDLIINPHA FPSRMTMGML IQSMAAKSGS LRGEFKTVET 970 980 990 1000 1010 1020 FQRYDDNDIV GHFGKELLDK GFNYHGNELM YSGIFGTPLK ADIFIGVVYY QRLRHMVSDK 1030 1040 1050 1060 1070 1080 SQARGTGPID ILTHQPVKGR KKGGGIRFGE MERDSLLAHG AAYCLNDRLF RSSDYSEGFV 1090 1100 1110 1120 1130 1140 CQNCGSILSC YVNRAIMKTQ TFIPPSLDES NKDTEDKEIH MNEKVICKVC KKNSNCKKVA 1150 1160 LPFVLRFLAN ELASMGIKLK FTVNDF

References

[1]	"TGA cysteine codons and intron sequences in conserved and nonconserved positions are found in macronuclear RNA polymerase genes of Euplotes octocarinatus." Kaufmann J., Florian V., Klein A. Nucleic Acids Res. 20:5985-5989(1992) [PubMed: 1461731] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: (68)-VIII.
[2]	"Gene dosage as a possible major determinant for equal expression levels of genes encoding RNA polymerase subunits in the hypotrichous ciliate Euplotes octocarinatus." Kaufmann J., Klein A. Nucleic Acids Res. 20:4445-4450(1992) [PubMed: 1408746] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-546. Strain: (68)-VIII.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X66451 Genomic DNA. Translation: CAA47067.1.
PIR	S70413.
3D structure databases
ProteinModelPortal	P28365.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR015712. DNA-dir_RNA_pol_su2. IPR007120. DNA-dir_RNA_pol_su2_6. IPR007121. RNA_pol_bsu_CS. IPR007644. RNA_pol_bsu_protrusion. IPR009674. RNA_pol_Rpa2-specific. IPR007642. RNA_pol_Rpb2_2. IPR007645. RNA_pol_Rpb2_3. IPR007647. RNA_pol_Rpb2_5. IPR007641. RNA_pol_Rpb2_7. [Graphical view]
Gene3D	G3DSA:2.40.270.10. G3DSA:2.40.270.10. 2 hits.
PANTHER	PTHR20856. RNA_pol_I_sub2. 1 hit.
Pfam	PF06883. RNA_pol_Rpa2_4. 1 hit. PF04563. RNA_pol_Rpb2_1. 1 hit. PF04561. RNA_pol_Rpb2_2. 1 hit. PF04565. RNA_pol_Rpb2_3. 1 hit. PF04567. RNA_pol_Rpb2_5. 1 hit. PF00562. RNA_pol_Rpb2_6. 1 hit. PF04560. RNA_pol_Rpb2_7. 1 hit. [Graphical view]
PROSITE	PS01166. RNA_POL_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPA2_EUPOC

Accession

Primary (citable) accession number: P28365

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	February 1, 1996
Last modified:	May 31, 2011
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)