Reviewed, UniProtKB/Swiss-Prot P28363 (RPA1_EUPOC)

Names and origin

Protein names

Recommended name:
    DNA-directed RNA polymerase I subunit RPA1
    EC=2.7.7.6
Alternative name(s):
    DNA-directed RNA polymerase I largest subunit

Gene names

Name:

RPA1

Organism

Euplotes octocarinatus

Taxonomic identifier

5937 [NCBI]

Taxonomic lineage

Eukaryota › Alveolata › Ciliophora › Intramacronucleata › Spirotrichea › Hypotrichia › Euplotida › Euplotidae › Euplotes

Protein attributes

Sequence length	638 AA.
Sequence status	Fragment.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. RNA polymerase I is essentially used to transcribe ribosomal DNA units.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Each class of RNA polymerase is assembled from 9 to 14 different polypeptides. This subunit is the largest component of RNA polymerase I.
Subcellular location	Nucleus.
Miscellaneous	Three distinct zinc-containing RNA polymerases are found in eukaryotic nuclei: polymerase I for the ribosomal RNA precursor, polymerase II for the mRNA precursor, and polymerase III for 5S and tRNA genes.
Sequence similarities	Belongs to the RNA polymerase beta' chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase Nucleus
Ligand	Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
Gene Ontology (GO)
Biological process	transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›638	›638	DNA-directed RNA polymerase I subunit RPA1		PRO_0000073927
Experimental info
	Non-terminal residue	638	1

Sequences

	Sequence		Length	Mass (Da)
	P28363-1 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: AB82F8BB9C22F5B5	FASTA	638	73,299
	10 20 30 40 50 60 MATIFDSLIS SRLDGTRFSF YNDEEIQQMS VKEIHNPMAY DELNNPTSHG ICDESMGVSA 70 80 90 100 110 120 LDKLSKCKTC GCDSVYCPGH MGHIKLTSTC YNPFTMKLLH SLLKSKCLVC HRLRISPKRI 130 140 150 160 170 180 ELFEIRLKLI KLGYLVEAEK LSDFNHFSLE SIDSAIRILR KKTKGKTNKK EEKEDSEEVE 190 200 210 220 230 240 EDKYAAKEAL ENLIQKEKEN REIFYSHISS ILQEDTPNES IGNAITVAIR DITKEIMNSV 250 260 270 280 290 300 VPSKCPHCDV CNPRIKKDGA TKFFQMPLSN RDTKAMLSSH GRIDMRIDIS TMGAISEYDE 310 320 330 340 350 360 EDDESSGESE VREGDEEQEK KQKYLSPIEV LEHMRRLWDV EDTLLNELFD NYKIFFMNNL 370 380 390 400 410 420 LVSQNRFRPE SSGGKQSGDD RDYLHAHSAM LTKIINANIA FRRTIELKEN LTDDRNLETE 430 440 450 460 470 480 SEKSKTQQID LSKTEITSKQ VIKAWAELQE SVNCYLDSSL AAKLENKEKP GLRQLLERKE 490 500 510 520 530 540 GIFRMKMMGK RVNFAGRSVI SPDPYISTDQ IGVPEFIART ITFPEPAKNI EKLKRCVING 550 560 570 580 590 600 AHKHPGANFI EYPNGERKAL ENMTLEEKKQ SSIIRKWRKI VYRHMQTGDP LLVNRQPTLH 610 620 630 KPSIMAHNAI ILPKEQTIRM HYSNCKSYNA DFDGDEMK

References

[1]	"Gene dosage as a possible major determinant for equal expression levels of genes encoding RNA polymerase subunits in the hypotrichous ciliate Euplotes octocarinatus." Kaufmann J., Klein A. Nucleic Acids Res. 20:4445-4450(1992) [PubMed: 1408746] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: (68)-VIII.
[2]	"TGA cysteine codons and intron sequences in conserved and nonconserved positions are found in macronuclear RNA polymerase genes of Euplotes octocarinatus." Kaufmann J., Florian V., Klein A. Nucleic Acids Res. 20:5985-5989(1992) [PubMed: 1461731] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-94.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X66450 Genomic DNA. Translation: CAA47066.1.
PIR	S33884.
3D structure databases
SMR	P28363. Positions 11-638.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.7.6. 294828.
Family and domain databases
InterPro	IPR015699. DNA-dir_RNA_pol1_lsu. IPR000722. RNA_pol_asu. IPR006592. RNA_pol_N. IPR007080. RNA_pol_Rpb1_1. [Graphical view]
Gene3D	G3DSA:2.40.40.30. RNA_pol_A. 1 hit. G3DSA:3.90.1120.10. RNA_pol_Rpb1_1. 1 hit.
PANTHER	PTHR19376:SF11. RNA_polyI. 1 hit.
Pfam	PF04997. RNA_pol_Rpb1_1. 1 hit. PF00623. RNA_pol_Rpb1_2. 1 hit. [Graphical view]
SMART	SM00663. RPOLA_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPA1_EUPOC

Accession

Primary (citable) accession number: P28363

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	January 19, 2010
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)