Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Homeobox protein Hox-D9

Gene

HOXD9

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi285 – 34460HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. RNA polymerase II regulatory region sequence-specific DNA binding Source: NTNU_SB
  2. RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: NTNU_SB

GO - Biological processi

  1. adult locomotory behavior Source: Ensembl
  2. anterior/posterior pattern specification Source: Ensembl
  3. embryonic forelimb morphogenesis Source: Ensembl
  4. embryonic skeletal system morphogenesis Source: Ensembl
  5. hindlimb morphogenesis Source: Ensembl
  6. mammary gland development Source: Ensembl
  7. negative regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
  8. peripheral nervous system neuron development Source: Ensembl
  9. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
  10. proximal/distal pattern formation Source: Ensembl
  11. single fertilization Source: Ensembl
  12. skeletal muscle tissue development Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein Hox-D9
Alternative name(s):
Homeobox protein Hox-4C
Homeobox protein Hox-5.2
Gene namesi
Name:HOXD9
Synonyms:HOX4C
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:5140. HOXD9.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA29414.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 352352Homeobox protein Hox-D9PRO_0000200220Add
BLAST

Proteomic databases

MaxQBiP28356.
PaxDbiP28356.
PRIDEiP28356.

PTM databases

PhosphoSiteiP28356.

Expressioni

Developmental stagei

Expressed in the developing limb buds.

Gene expression databases

BgeeiP28356.
CleanExiHS_HOXD9.
GenevestigatoriP28356.

Interactioni

Protein-protein interaction databases

BioGridi109475. 8 interactions.
IntActiP28356. 3 interactions.
STRINGi9606.ENSP00000249499.

Structurei

3D structure databases

ProteinModelPortaliP28356.
SMRiP28356. Positions 285-344.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi125 – 15935Gly-richAdd
BLAST
Compositional biasi131 – 14010Poly-Gly
Compositional biasi175 – 18814Ser/Thr-richAdd
BLAST

Sequence similaritiesi

Belongs to the Abd-B homeobox family.Curated
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG286554.
GeneTreeiENSGT00760000118834.
HOGENOMiHOG000231180.
HOVERGENiHBG016849.
InParanoidiP28356.
KOiK09294.
OMAiSGPEFSC.
OrthoDBiEOG722J9F.
PhylomeDBiP28356.
TreeFamiTF317819.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
IPR006711. Hox9_activation_N.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF04617. Hox9_act. 1 hit.
[Graphical view]
PRINTSiPR00024. HOMEOBOX.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28356-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLGGSAGRLK MSSSGTLSNY YVDSLIGHEG DEVFAARFGP PGPGAQGRPA
60 70 80 90 100
GVADGPAATA AEFASCSFAP RSAVFSASWS AVPSQPPAAA AMSGLYHPYV
110 120 130 140 150
PPPPLAASAS EPGRYVRSWM EPLPGFPGGA GGGGGGGGGG PGRGPSPGPS
160 170 180 190 200
GPANGRHYGI KPETRAAPAP ATAASTTSSS STSLSSSSKR TECSVARESQ
210 220 230 240 250
GSSGPEFSCN SFLQEKAAAA TGGTGPGAGI GAATGTGGSS EPSACSDHPI
260 270 280 290 300
PGCSLKEEEK QHSQPQQQQL DPNNPAANWI HARSTRKKRC PYTKYQTLEL
310 320 330 340 350
EKEFLFNMYL TRDRRYEVAR ILNLTERQVK IWFQNRRMKM KKMSKEKCPK

GD
Length:352
Mass (Da):36,495
Last modified:April 19, 2010 - v5
Checksum:iECBFE68B48C0F532
GO

Sequence cautioni

The sequence AAH44855.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAA42016.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti144 – 15613GPSPG…PANGR → VPALAPAAQPTGC in AAH44855 (PubMed:15489334).CuratedAdd
BLAST
Sequence conflicti219 – 2246AATGGT → GGDGGN in CAA42016 (PubMed:1756725).Curated
Sequence conflicti276 – 2761A → E in CAA42016 (PubMed:1756725).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti269 – 2691Missing .1 Publication
VAR_031851

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59372 mRNA. Translation: CAA42016.1. Different initiation.
AC009336 Genomic DNA. No translation available.
BC044855 mRNA. Translation: AAH44855.2. Different initiation.
X15506 Genomic DNA. Translation: CAA33528.1.
CCDSiCCDS2267.2.
PIRiS18649.
RefSeqiNP_055028.3. NM_014213.3.
UniGeneiHs.236646.

Genome annotation databases

EnsembliENST00000249499; ENSP00000249499; ENSG00000128709.
GeneIDi3235.
KEGGihsa:3235.
UCSCiuc010zex.2. human.

Polymorphism databases

DMDMi294862543.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59372 mRNA. Translation: CAA42016.1. Different initiation.
AC009336 Genomic DNA. No translation available.
BC044855 mRNA. Translation: AAH44855.2. Different initiation.
X15506 Genomic DNA. Translation: CAA33528.1.
CCDSiCCDS2267.2.
PIRiS18649.
RefSeqiNP_055028.3. NM_014213.3.
UniGeneiHs.236646.

3D structure databases

ProteinModelPortaliP28356.
SMRiP28356. Positions 285-344.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109475. 8 interactions.
IntActiP28356. 3 interactions.
STRINGi9606.ENSP00000249499.

PTM databases

PhosphoSiteiP28356.

Polymorphism databases

DMDMi294862543.

Proteomic databases

MaxQBiP28356.
PaxDbiP28356.
PRIDEiP28356.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000249499; ENSP00000249499; ENSG00000128709.
GeneIDi3235.
KEGGihsa:3235.
UCSCiuc010zex.2. human.

Organism-specific databases

CTDi3235.
GeneCardsiGC02P176987.
HGNCiHGNC:5140. HOXD9.
MIMi142982. gene.
neXtProtiNX_P28356.
PharmGKBiPA29414.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG286554.
GeneTreeiENSGT00760000118834.
HOGENOMiHOG000231180.
HOVERGENiHBG016849.
InParanoidiP28356.
KOiK09294.
OMAiSGPEFSC.
OrthoDBiEOG722J9F.
PhylomeDBiP28356.
TreeFamiTF317819.

Miscellaneous databases

GeneWikiiHOXD9.
GenomeRNAii3235.
NextBioi12875.
PROiP28356.
SOURCEiSearch...

Gene expression databases

BgeeiP28356.
CleanExiHS_HOXD9.
GenevestigatoriP28356.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
IPR006711. Hox9_activation_N.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF04617. Hox9_act. 1 hit.
[Graphical view]
PRINTSiPR00024. HOMEOBOX.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "HOX4 genes encode transcription factors with potential auto- and cross-regulatory capacities."
    Zappavigna V., Renucci A., Izpisua-Belmonte J.-C., Urier G., Peschle C., Duboule D.
    EMBO J. 10:4177-4187(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Spinal cord.
  2. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Colon.
  4. "Complementary homeo protein gradients in developing limb buds."
    Oliver G., Sidell N., Fiske N., Heinzmann C., Mohandas T., Sparkes R.S., De Robertis E.M.
    Genes Dev. 3:641-650(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 274-352.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 285-350.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. Cited for: VARIANT GLN-269 DEL.

Entry informationi

Entry nameiHXD9_HUMAN
AccessioniPrimary (citable) accession number: P28356
Secondary accession number(s): Q86ST1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 1992
Last sequence update: April 19, 2010
Last modified: March 31, 2015
This is version 133 of the entry and version 5 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-11 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Vertebrate homeotic Hox proteins
    Nomenclature of vertebrate homeotic Hox proteins and list of entries
  2. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.