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Reviewed, UniProtKB/Swiss-Prot P28352 (APEX1_MOUSE)

Last modified November 3, 2009. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    DNA-(apurinic or apyrimidinic site) lyase
    EC=4.2.99.18
Alternative name(s):
    Apurinic-apyrimidinic endonuclease 1
      Short name=AP endonuclease 1
    APEX nuclease
      Short name=APEN
Gene names
Name: Apex1
Synonyms: Ape, Apex
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMus

Protein attributes

Sequence length317 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Repairs oxidative DNA damages in vitro. May have a role in protection against cell lethality and suppression of mutations. Removes the blocking groups from the 3'-termini of the DNA strand breaks generated by ionizing radiations and bleomycin.

Catalytic activity

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.

Subunit structure

Monomer. Component of the SET complex, which also contains SET, ANP32A, HMGB2 and NME1 By similarity.

Subcellular location

Nucleus.

Sequence similarities

Belongs to the DNA repair enzymes AP/exoA family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.5
Chain2 – 317316DNA-(apurinic or apyrimidinic site) lyase
PRO_0000200011

Sites

Active site3081Proton acceptor By similarity
Metal binding671Magnesium or manganese By similarity
Metal binding951Magnesium or manganese By similarity
Metal binding2091Magnesium or manganese By similarity
Metal binding2111Magnesium or manganese By similarity
Metal binding3071Magnesium or manganese By similarity
Metal binding3081Magnesium or manganese By similarity
Site2111Important for substrate recognition By similarity

Amino acid modifications

Modified residue1961N6-acetyllysine By similarity
Modified residue2611Phosphotyrosine By similarity

Sequences

Sequence LengthMass (Da)Tools
P28352-1 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: CF086691FAC89C4A

FASTA31735,490
        10         20         30         40         50         60 
MPKRGKKAAA DDGEEPKSEP ETKKSKGAAK KTEKEAAGEG PVLYEDPPDQ KTSPSGKSAT 

        70         80         90        100        110        120 
LKICSWNVDG LRAWIKKKGL DWVKEEAPDI LCLQETKCSE NKLPAELQEL PGLTHQYWSA 

       130        140        150        160        170        180 
PSDKEGYSGV GLLSRQCPLK VSYGIGEEEH DQEGRVIVAE FESFVLVTAY VPNAGRGLVR 

       190        200        210        220        230        240 
LEYRQRWDEA FRKFLKDLAS RKPLVLCGDL NVAHEEIDLR NPKGNKKNAG FTPQERQGFG 

       250        260        270        280        290        300 
ELLQAVPLAD SFRHLYPNTA YAYTFWTYMM NARSKNVGWR LDYFLLSHSL LPALCDSKIR 

       310 
SKALGSDHCP ITLYLAL 

« Hide

References

« Hide 'large scale' references
[1]"cDNA and deduced amino acid sequence of a mouse DNA repair enzyme (APEX nuclease) with significant homology to Escherichia coli exonuclease III."
Seki S., Akiyama K., Watanabe S., Hatsushika M., Ikeda S., Tsutsui K.
J. Biol. Chem. 266:20797-20802(1991) [PubMed: 1939131] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Genomic structure of the mouse apurinic/apyrimidinic endonuclease gene."
Takiguchi Y., Chen D.J.
Mamm. Genome 5:717-722(1994) [PubMed: 7533013] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129.
Tissue: Embryo.
[3]"Cloning, sequence analysis, and chromosomal assignment of the mouse Apex gene."
Akiyama K., Nagao K., Oshida T., Tsutsui K., Yoshida M.C., Seki S.
Genomics 26:63-69(1995) [PubMed: 7782087] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: BALB/c.
Tissue: Blood.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[5]"A mouse DNA repair enzyme (APEX nuclease) having exonuclease and apurinic/apyrimidinic endonuclease activities: purification and characterization."
Seki S., Ikeda S., Watanabe S., Hatsushika M., Tsutsui K., Akiyama K., Zhang B.
Biochim. Biophys. Acta 1079:57-64(1991) [PubMed: 1716153] [Abstract]
Cited for: PARTIAL PROTEIN SEQUENCE OF 2-22, CHARACTERIZATION.
Tissue: Ascites.
+Additional computationally mapped references.

Cross-references

Sequence databases

D90374 mRNA. Translation: BAA14382.1.
U12273 Genomic DNA. Translation: AAC13769.1.
D38077 Genomic DNA. Translation: BAA07270.1.
BC052401 mRNA. Translation: AAH52401.1.
IPIIPI00224152.
PIRA39500.
RefSeqNP_033817.1.
UniGeneMm.203

3D structure databases

HSSPHSSP built from PDB template 1BIX based on UniProtKB P27695.
SMRP28352. Positions 39-317.
ModBaseSearch...

Protein-protein interaction databases

STRINGP28352.

PTM databases

PhosphoSiteP28352.

Proteomic databases

PRIDEP28352.

Genome annotation databases

EnsemblENSMUST00000049411; ENSMUSP00000042602; ENSMUSG00000035960; Mus musculus. [Genome view]
GeneID11792.
KEGGmmu:11792.
UCSCuc007tly.1. mouse.

Organism-specific databases

CTD11792.
MGIMGI:88042. Apex1.

Phylogenomic databases

HOGENOMP28352.
HOVERGENP28352.
OMAYTWWSYM.

Enzyme and pathway databases

BRENDA4.2.99.18. 244.

Gene expression databases

ArrayExpressP28352.
BgeeP28352.
CleanExMM_APEX1.
GenevestigatorP28352.
GermOnlineENSMUSG00000035960. Mus musculus.

Family and domain databases

InterProIPR000097. AP_endonuclease_F1.
IPR005135. Endo/exonuclease/phosphatase.
IPR004808. exoDNase_III.
[Graphical view]
PANTHERPTHR22748. ExoIII_xth. 1 hit.
PfamPF03372. Exo_endo_phos. 1 hit.
[Graphical view]
TIGRFAMsTIGR00195. exoDNase_III. 1 hit.
TIGR00633. xth. 1 hit.
PROSITEPS00726. AP_NUCLEASE_F1_1. 1 hit.
PS00727. AP_NUCLEASE_F1_2. 1 hit.
PS00728. AP_NUCLEASE_F1_3. 1 hit.
PS51435. AP_NUCLEASE_F1_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio279621.
SOURCESearch...

Entry information

Entry nameAPEX1_MOUSE
AccessionPrimary (citable) accession number: P28352
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: January 23, 2007
Last modified: November 3, 2009
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents