Reviewed, UniProtKB/Swiss-Prot P28351 (AGAL_ASPNG)

Names and origin

Protein names

Recommended name:
    Alpha-galactosidase A
    EC=3.2.1.22
Alternative name(s):
    Melibiase

Gene names

Name:

aglA

Organism

Aspergillus niger

Taxonomic identifier

5061 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	545 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Represents a minor extracellular alpha-galactosidase activity in A.niger.
Catalytic activity	Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactohydrolase.
Subcellular location	Secreted.
Post-translational modification	A C-terminal Ser/Thr-rich region may provide possible sites for O-glycosylation.
Sequence similarities	Belongs to the glycosyl hydrolase 27 family. Contains 1 ricin B-type lectin domain.
Caution	It is uncertain whether Met-1 or Met-9 is the initiator.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Lectin
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alpha-galactosidase activity Inferred from electronic annotation. Source: EC sugar binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Ref.1
	Chain	32 – 545	514	Alpha-galactosidase A		PRO_0000000999
Regions
	Domain	421 – 518	98	Ricin B-type lectin
Sites
	Active site	162	1	Nucleophile By similarity
	Active site	220	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	57	1	N-linked (GlcNAc...) Potential
	Glycosylation	95	1	N-linked (GlcNAc...) Potential
	Glycosylation	101	1	N-linked (GlcNAc...) Potential
	Glycosylation	131	1	N-linked (GlcNAc...) Potential
	Glycosylation	211	1	N-linked (GlcNAc...) Potential
	Glycosylation	363	1	N-linked (GlcNAc...) Potential
	Glycosylation	444	1	N-linked (GlcNAc...) Potential
	Disulfide bond	54 ↔ 86		By similarity
	Disulfide bond	134 ↔ 164		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P28351-1 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 2DC5A710CE95D59C	FASTA	545	60,148
	10 20 30 40 50 60 MIQGLESIMN QGTKRILLAA TLAATPWQVY GSIEQPSLLP TPPMGFNNWA RFMCDLNETL 70 80 90 100 110 120 FTETADTMAA NGLRDAGYNR INLDDCWMAY QRSDNGSLQW NTTKFPHGLP WLAKYVKAKG 130 140 150 160 170 180 FHFGIYEDSG NMTCGGYPGS YNHEEQDANT FASWGIDYLK LDGCNVYATQ GRTLEEEYKQ 190 200 210 220 230 240 RYGHWHQVLS KMQHPLIFSE SAPAYFAGTD NNTDWYTVMD WVPIYGELAR HSTDILVYSG 250 260 270 280 290 300 AGSAWDSIMN NYNYNTLLAR YQRPGYFNDP DFLIPDHPGL TADEKRSHFA LWASFSAPLI 310 320 330 340 350 360 ISAYIPALSK DEIAFLTNEA LIAVNQDPLA QQATLASRDD TLDILTRSLA NGDRLLTVLN 370 380 390 400 410 420 KGNTTVTRDI PVQWLGLTET DCTYTAEDLW DGKTQKISDH IKIELASHAT AVFRLSLPQG 430 440 450 460 470 480 CSSVVPTGLV FNTASGNCLT AASNSSVAFQ SCNGETSQIW QVTPSGVIRP VSQTTQCLAA 490 500 510 520 530 540 DGNLVKLQAC DSTDSDGQKW TYPVTGNLKN AKTDGCLTEG SVQMKSCLYE RDGQVFGLPS GVQLA

References

[1]

"Cloning and expression of a member of the Aspergillus niger gene family encoding alpha-galactosidase." den Herder I.F., Rosell A.M.M., van Zuilen C.M., Punt P.J., van den Hondel C.A.M.J.J. Mol. Gen. Genet. 233:404-410(1992) [PubMed: 1320186] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 32-59. Strain: ATCC 9089 / N402.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X63348 Genomic DNA. Translation: CAA44950.1.
PIR	S23582.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	CBM13. Carbohydrate-Binding Module Family 13. GH27. Glycoside Hydrolase Family 27.
PTM databases
GlycoSuiteDB	P28351.
Enzyme and pathway databases
BRENDA	3.2.1.22. 277.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR002241. Glyco_hydro_27. IPR000111. Glyco_hydro_GHD. IPR000772. Ricin_B_lectin. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF02065. Melibiase. 1 hit. PF00652. Ricin_B_lectin. 1 hit. [Graphical view]
PRINTS	PR00740. GLHYDRLASE27.
ProDom	PD002572. Glyco_hydro_GHD. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00458. RICIN. 1 hit. [Graphical view]
PROSITE	PS00512. ALPHA_GALACTOSIDASE. 1 hit. PS50231. RICIN_B_LECTIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AGAL_ASPNG

Accession

Primary (citable) accession number: P28351

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	June 16, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)