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P28347 (TEAD1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 141. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcriptional enhancer factor TEF-1
Alternative name(s):
NTEF-1
Protein GT-IIC
TEA domain family member 1
Short name=TEAD-1
Transcription factor 13
Short name=TCF-13
Gene names
Name:TEAD1
Synonyms:TCF13, TEF1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length426 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and cooperatively to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription in vivo in a cell-specific manner. The activation function appears to be mediated by a limiting cell-specific transcriptional intermediary factor (TIF). Involved in cardiac development. Binds to the M-CAT motif. Ref.4 Ref.5

Subunit structure

Interacts with YAP1 and WWTR1/TAZ. Ref.4 Ref.5

Subcellular location

Nucleus.

Tissue specificity

Preferentially expressed in skeletal muscle. Lower levels in pancreas, placenta, and heart.

Involvement in disease

Sveinsson chorioretinal atrophy (SCRA) [MIM:108985]: Characterized by symmetrical lesions radiating from the optic disk involving the retina and the choroid.
Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.4 Ref.9 Ref.10

Sequence similarities

Contains 1 TEA DNA-binding domain.

Caution

It is uncertain whether Met-1 or Met-16 is the initiator.

Sequence caution

The sequence AAB00791.1 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical ATT isoleucine codon.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

vgQ263663EBI-529156,EBI-162687From a different organism.
YAP1P469373EBI-529156,EBI-1044059

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 426426Transcriptional enhancer factor TEF-1
PRO_0000205930

Regions

DNA binding30 – 9768TEA
Region167 – 426260Transcriptional activation Potential
Compositional bias143 – 20462Pro-rich
Compositional bias306 – 32823Ser/Thr/Tyr-rich

Amino acid modifications

Modified residue11N-acetylmethionine Ref.6 Ref.7

Natural variations

Natural variant4211Y → H in SCRA; loss of interaction with YAP1 and also activation by YAP1. Ref.4 Ref.9 Ref.10
Corresponds to variant rs11567847 [ dbSNP | Ensembl ].
VAR_031530

Experimental info

Mutagenesis4211Y → A: Important loss of interaction with YAP1. Ref.9

Secondary structure

.................................................. 426
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P28347 [UniParc].

Last modified July 15, 1998. Version 2.
Checksum: EFD8067F2770B3C7

FASTA42647,946
        10         20         30         40         50         60 
MEPSSWSGSE SPAENMERMS DSADKPIDND AEGVWSPDIE QSFQEALAIY PPCGRRKIIL 

        70         80         90        100        110        120 
SDEGKMYGRN ELIARYIKLR TGKTRTRKQV SSHIQVLARR KSRDFHSKLK DQTAKDKALQ 

       130        140        150        160        170        180 
HMAAMSSAQI VSATAIHNKL GLPGIPRPTF PGAPGFWPGM IQTGQPGSSQ DVKPFVQQAY 

       190        200        210        220        230        240 
PIQPAVTAPI PGFEPASAPA PSVPAWQGRS IGTTKLRLVE FSAFLEQQRD PDSYNKHLFV 

       250        260        270        280        290        300 
HIGHANHSYS DPLLESVDIR QIYDKFPEKK GGLKELFGKG PQNAFFLVKF WADLNCNIQD 

       310        320        330        340        350        360 
DAGAFYGVTS QYESSENMTV TCSTKVCSFG KQVVEKVETE YARFENGRFV YRINRSPMCE 

       370        380        390        400        410        420 
YMINFIHKLK HLPEKYMMNS VLENFTILLV VTNRDTQETL LCMACVFEVS NSEHGAQHHI 


YRLVKD 

« Hide

References

« Hide 'large scale' references
[1]"Cloning, expression, and transcriptional properties of the human enhancer factor TEF-1."
Xiao J.H., Davidson I., Matthes H., Garnier J.-M., Chambon P.
Cell 65:551-568(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]"Human chromosome 11 DNA sequence and analysis including novel gene identification."
Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. expand/collapse author list , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y.
Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The TEA domain: a novel, highly conserved DNA-binding motif."
Buerglin T.R.
Cell 66:11-12(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: DOMAIN TEA.
[4]"TEAD mediates YAP-dependent gene induction and growth control."
Zhao B., Ye X., Yu J., Li L., Li W., Li S., Yu J., Lin J.D., Wang C.Y., Chinnaiyan A.M., Lai Z.C., Guan K.L.
Genes Dev. 22:1962-1971(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH YAP1, CHARACTERIZATION OF VARIANT SCRA HIS-421.
[5]"TEAD transcription factors mediate the function of TAZ in cell growth and epithelial-mesenchymal transition."
Zhang H., Liu C.Y., Zha Z.Y., Zhao B., Yao J., Zhao S., Xiong Y., Lei Q.Y., Guan K.L.
J. Biol. Chem. 284:13355-13362(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, INTERACTION WITH WWTR1.
[6]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[7]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Insights into transcription enhancer factor 1 (TEF-1) activity from the solution structure of the TEA domain."
Anbanandam A., Albarado D.C., Nguyen C.T., Halder G., Gao X., Veeraraghavan S.
Proc. Natl. Acad. Sci. U.S.A. 103:17225-17230(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 28-104.
[9]"Structural insights into the YAP and TEAD complex."
Li Z., Zhao B., Wang P., Chen F., Dong Z., Yang H., Guan K.L., Xu Y.
Genes Dev. 24:235-240(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 209-426 IN COMPLEX WITH YAP1, CHARACTERIZATION OF VARIANT SCRA HIS-421, MUTAGENESIS OF TYR-421.
[10]"A novel TEAD1 mutation is the causative allele in Sveinsson's chorioretinal atrophy (helicoid peripapillary chorioretinal degeneration)."
Fossdal R., Jonasson F., Kristjansdottir G.T., Kong A., Stefansson H., Gosh S., Gulcher J.R., Stefansson K.
Hum. Mol. Genet. 13:975-981(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT SCRA HIS-421.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M63896 Genomic DNA. Translation: AAB00791.1. Sequence problems.
AC013549 Genomic DNA. No translation available.
AC084859 Genomic DNA. No translation available.
AC107881 Genomic DNA. No translation available.
PIRA40032.
RefSeqNP_068780.2. NM_021961.5.
UniGeneHs.655331.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2HZDNMR-A28-104[»]
3KYSX-ray2.80A/C209-426[»]
ProteinModelPortalP28347.
SMRP28347. Positions 27-105, 209-425.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112862. 13 interactions.
IntActP28347. 6 interactions.
MINTMINT-1512394.
STRING9606.ENSP00000354588.

PTM databases

PhosphoSiteP28347.

Polymorphism databases

DMDM3041733.

Proteomic databases

PaxDbP28347.
PRIDEP28347.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000361905; ENSP00000355332; ENSG00000187079.
GeneID7003.
KEGGhsa:7003.
UCSCuc021qdx.1. human.

Organism-specific databases

CTD7003.
GeneCardsGC11P012652.
H-InvDBHIX0026218.
HGNCHGNC:11714. TEAD1.
HPAHPA046053.
MIM108985. phenotype.
189967. gene.
neXtProtNX_P28347.
Orphanet86813. Helicoid peripapillary chorioretinal degeneration.
PharmGKBPA36432.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG246992.
HOGENOMHOG000253933.
HOVERGENHBG056905.
InParanoidP28347.
KOK09448.
OrthoDBEOG7288RP.
PhylomeDBP28347.

Enzyme and pathway databases

ReactomeREACT_71. Gene Expression.
SignaLinkP28347.

Gene expression databases

ArrayExpressP28347.
BgeeP28347.
CleanExHS_TEAD1.
GenevestigatorP28347.

Family and domain databases

InterProIPR000818. TEA/ATTS.
IPR016361. TEF.
[Graphical view]
PANTHERPTHR11834. PTHR11834. 1 hit.
PfamPF01285. TEA. 1 hit.
[Graphical view]
PIRSFPIRSF002603. TEF. 1 hit.
PRINTSPR00065. TEADOMAIN.
SMARTSM00426. TEA. 1 hit.
[Graphical view]
PROSITEPS00554. TEA_1. 1 hit.
PS51088. TEA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSTEAD1. human.
EvolutionaryTraceP28347.
GeneWikiTEAD1.
GenomeRNAi7003.
NextBio27350.
PROP28347.
SOURCESearch...

Entry information

Entry nameTEAD1_HUMAN
AccessionPrimary (citable) accession number: P28347
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: July 15, 1998
Last modified: April 16, 2014
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM