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P28347

- TEAD1_HUMAN

UniProt

P28347 - TEAD1_HUMAN

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Protein

Transcriptional enhancer factor TEF-1

Gene

TEAD1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and cooperatively to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription in vivo in a cell-specific manner. The activation function appears to be mediated by a limiting cell-specific transcriptional intermediary factor (TIF). Involved in cardiac development. Binds to the M-CAT motif.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi30 – 9768TEAPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: ProtInc
  2. sequence-specific DNA binding transcription factor activity Source: UniProtKB

GO - Biological processi

  1. gene expression Source: Reactome
  2. hippo signaling Source: UniProtKB
  3. transcription initiation from RNA polymerase II promoter Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_116145. PPARA activates gene expression.
REACT_118713. YAP1- and WWTR1 (TAZ)-stimulated gene expression.
SignaLinkiP28347.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional enhancer factor TEF-1
Alternative name(s):
NTEF-1
Protein GT-IIC
TEA domain family member 1
Short name:
TEAD-1
Transcription factor 13
Short name:
TCF-13
Gene namesi
Name:TEAD1
Synonyms:TCF13, TEF1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 11

Organism-specific databases

HGNCiHGNC:11714. TEAD1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: HPA
  2. Golgi apparatus Source: HPA
  3. nucleoplasm Source: Reactome
  4. nucleus Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Involvement in diseasei

Sveinsson chorioretinal atrophy (SCRA) [MIM:108985]: Characterized by symmetrical lesions radiating from the optic disk involving the retina and the choroid.1 Publication
Note: The disease is caused by mutations affecting the gene represented in this entry.
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti421 – 4211Y → H in SCRA; loss of interaction with YAP1 and also activation by YAP1. 1 Publication
Corresponds to variant rs11567847 [ dbSNP | Ensembl ].
VAR_031530

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi421 – 4211Y → A: Important loss of interaction with YAP1. 1 Publication

Keywords - Diseasei

Disease mutation

Organism-specific databases

MIMi108985. phenotype.
Orphaneti86813. Helicoid peripapillary chorioretinal degeneration.
PharmGKBiPA36432.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 426426Transcriptional enhancer factor TEF-1PRO_0000205930Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine2 Publications

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP28347.
PaxDbiP28347.
PRIDEiP28347.

PTM databases

PhosphoSiteiP28347.

Expressioni

Tissue specificityi

Preferentially expressed in skeletal muscle. Lower levels in pancreas, placenta, and heart.

Gene expression databases

BgeeiP28347.
CleanExiHS_TEAD1.
GenevestigatoriP28347.

Organism-specific databases

HPAiHPA046053.

Interactioni

Subunit structurei

Interacts with YAP1 and WWTR1/TAZ.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
vgQ263663EBI-529156,EBI-162687From a different organism.
YAP1P469375EBI-529156,EBI-1044059

Protein-protein interaction databases

BioGridi112862. 16 interactions.
IntActiP28347. 6 interactions.
MINTiMINT-1512394.
STRINGi9606.ENSP00000354588.

Structurei

Secondary structure

1
426
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi29 – 313
Helixi37 – 4913
Beta strandi52 – 543
Helixi60 – 634
Helixi70 – 8112
Helixi88 – 10114
Beta strandi216 – 23015
Beta strandi233 – 24210
Beta strandi256 – 2583
Helixi259 – 2613
Helixi263 – 2653
Helixi273 – 2797
Helixi282 – 2843
Beta strandi285 – 2928
Beta strandi303 – 31614
Beta strandi319 – 32810
Beta strandi331 – 34111
Beta strandi343 – 3453
Beta strandi348 – 35710
Helixi360 – 37011
Helixi375 – 3828
Beta strandi385 – 3939
Turni394 – 3963
Beta strandi399 – 40911
Beta strandi412 – 4143
Beta strandi417 – 4248

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2HZDNMR-A28-104[»]
3KYSX-ray2.80A/C209-426[»]
ProteinModelPortaliP28347.
SMRiP28347. Positions 27-105, 209-425.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP28347.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni167 – 426260Transcriptional activationSequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi143 – 20462Pro-richAdd
BLAST
Compositional biasi306 – 32823Ser/Thr/Tyr-richAdd
BLAST

Sequence similaritiesi

Contains 1 TEA DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG246992.
GeneTreeiENSGT00390000008670.
HOGENOMiHOG000253933.
HOVERGENiHBG056905.
InParanoidiP28347.
KOiK09448.
OrthoDBiEOG7288RP.
PhylomeDBiP28347.

Family and domain databases

InterProiIPR000818. TEA/ATTS.
IPR016361. TEF.
[Graphical view]
PANTHERiPTHR11834. PTHR11834. 1 hit.
PfamiPF01285. TEA. 1 hit.
[Graphical view]
PIRSFiPIRSF002603. TEF. 1 hit.
PRINTSiPR00065. TEADOMAIN.
SMARTiSM00426. TEA. 1 hit.
[Graphical view]
PROSITEiPS00554. TEA_1. 1 hit.
PS51088. TEA_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P28347-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEPSSWSGSE SPAENMERMS DSADKPIDND AEGVWSPDIE QSFQEALAIY
60 70 80 90 100
PPCGRRKIIL SDEGKMYGRN ELIARYIKLR TGKTRTRKQV SSHIQVLARR
110 120 130 140 150
KSRDFHSKLK DQTAKDKALQ HMAAMSSAQI VSATAIHNKL GLPGIPRPTF
160 170 180 190 200
PGAPGFWPGM IQTGQPGSSQ DVKPFVQQAY PIQPAVTAPI PGFEPASAPA
210 220 230 240 250
PSVPAWQGRS IGTTKLRLVE FSAFLEQQRD PDSYNKHLFV HIGHANHSYS
260 270 280 290 300
DPLLESVDIR QIYDKFPEKK GGLKELFGKG PQNAFFLVKF WADLNCNIQD
310 320 330 340 350
DAGAFYGVTS QYESSENMTV TCSTKVCSFG KQVVEKVETE YARFENGRFV
360 370 380 390 400
YRINRSPMCE YMINFIHKLK HLPEKYMMNS VLENFTILLV VTNRDTQETL
410 420
LCMACVFEVS NSEHGAQHHI YRLVKD
Length:426
Mass (Da):47,946
Last modified:July 15, 1998 - v2
Checksum:iEFD8067F2770B3C7
GO
Isoform 2 (identifier: P28347-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-15: Missing.
     110-110: K → KVTSM
     234-291: Missing.

Note: No experimental confirmation available.

Show »
Length:357
Mass (Da):40,000
Checksum:i0CCACF105ABDFFD5
GO

Sequence cautioni

The sequence AAB00791.1 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical ATT isoleucine codon.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti421 – 4211Y → H in SCRA; loss of interaction with YAP1 and also activation by YAP1. 1 Publication
Corresponds to variant rs11567847 [ dbSNP | Ensembl ].
VAR_031530

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1515Missing in isoform 2. 1 PublicationVSP_056275Add
BLAST
Alternative sequencei110 – 1101K → KVTSM in isoform 2. 1 PublicationVSP_056276
Alternative sequencei234 – 29158Missing in isoform 2. 1 PublicationVSP_056277Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M63896 Genomic DNA. Translation: AAB00791.1. Sequence problems.
AC013549 Genomic DNA. No translation available.
AC084859 Genomic DNA. No translation available.
AC107881 Genomic DNA. No translation available.
BC115398 mRNA. Translation: AAI15399.1.
CCDSiCCDS7810.2. [P28347-1]
PIRiA40032.
RefSeqiNP_068780.2. NM_021961.5.
UniGeneiHs.655331.

Genome annotation databases

EnsembliENST00000334310; ENSP00000334754; ENSG00000187079. [P28347-2]
GeneIDi7003.
KEGGihsa:7003.
UCSCiuc021qdx.1. human. [P28347-1]

Polymorphism databases

DMDMi3041733.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M63896 Genomic DNA. Translation: AAB00791.1 . Sequence problems.
AC013549 Genomic DNA. No translation available.
AC084859 Genomic DNA. No translation available.
AC107881 Genomic DNA. No translation available.
BC115398 mRNA. Translation: AAI15399.1 .
CCDSi CCDS7810.2. [P28347-1 ]
PIRi A40032.
RefSeqi NP_068780.2. NM_021961.5.
UniGenei Hs.655331.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2HZD NMR - A 28-104 [» ]
3KYS X-ray 2.80 A/C 209-426 [» ]
ProteinModelPortali P28347.
SMRi P28347. Positions 27-105, 209-425.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 112862. 16 interactions.
IntActi P28347. 6 interactions.
MINTi MINT-1512394.
STRINGi 9606.ENSP00000354588.

PTM databases

PhosphoSitei P28347.

Polymorphism databases

DMDMi 3041733.

Proteomic databases

MaxQBi P28347.
PaxDbi P28347.
PRIDEi P28347.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000334310 ; ENSP00000334754 ; ENSG00000187079 . [P28347-2 ]
GeneIDi 7003.
KEGGi hsa:7003.
UCSCi uc021qdx.1. human. [P28347-1 ]

Organism-specific databases

CTDi 7003.
GeneCardsi GC11P012652.
H-InvDB HIX0026218.
HGNCi HGNC:11714. TEAD1.
HPAi HPA046053.
MIMi 108985. phenotype.
189967. gene.
neXtProti NX_P28347.
Orphaneti 86813. Helicoid peripapillary chorioretinal degeneration.
PharmGKBi PA36432.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG246992.
GeneTreei ENSGT00390000008670.
HOGENOMi HOG000253933.
HOVERGENi HBG056905.
InParanoidi P28347.
KOi K09448.
OrthoDBi EOG7288RP.
PhylomeDBi P28347.

Enzyme and pathway databases

Reactomei REACT_116145. PPARA activates gene expression.
REACT_118713. YAP1- and WWTR1 (TAZ)-stimulated gene expression.
SignaLinki P28347.

Miscellaneous databases

ChiTaRSi TEAD1. human.
EvolutionaryTracei P28347.
GeneWikii TEAD1.
GenomeRNAii 7003.
NextBioi 27350.
PROi P28347.
SOURCEi Search...

Gene expression databases

Bgeei P28347.
CleanExi HS_TEAD1.
Genevestigatori P28347.

Family and domain databases

InterProi IPR000818. TEA/ATTS.
IPR016361. TEF.
[Graphical view ]
PANTHERi PTHR11834. PTHR11834. 1 hit.
Pfami PF01285. TEA. 1 hit.
[Graphical view ]
PIRSFi PIRSF002603. TEF. 1 hit.
PRINTSi PR00065. TEADOMAIN.
SMARTi SM00426. TEA. 1 hit.
[Graphical view ]
PROSITEi PS00554. TEA_1. 1 hit.
PS51088. TEA_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, expression, and transcriptional properties of the human enhancer factor TEF-1."
    Xiao J.H., Davidson I., Matthes H., Garnier J.-M., Chambon P.
    Cell 65:551-568(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  4. "The TEA domain: a novel, highly conserved DNA-binding motif."
    Buerglin T.R.
    Cell 66:11-12(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOMAIN TEA.
  5. "TEAD mediates YAP-dependent gene induction and growth control."
    Zhao B., Ye X., Yu J., Li L., Li W., Li S., Yu J., Lin J.D., Wang C.Y., Chinnaiyan A.M., Lai Z.C., Guan K.L.
    Genes Dev. 22:1962-1971(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH YAP1, CHARACTERIZATION OF VARIANT SCRA HIS-421.
  6. "TEAD transcription factors mediate the function of TAZ in cell growth and epithelial-mesenchymal transition."
    Zhang H., Liu C.Y., Zha Z.Y., Zhao B., Yao J., Zhao S., Xiong Y., Lei Q.Y., Guan K.L.
    J. Biol. Chem. 284:13355-13362(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, INTERACTION WITH WWTR1.
  7. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Insights into transcription enhancer factor 1 (TEF-1) activity from the solution structure of the TEA domain."
    Anbanandam A., Albarado D.C., Nguyen C.T., Halder G., Gao X., Veeraraghavan S.
    Proc. Natl. Acad. Sci. U.S.A. 103:17225-17230(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 28-104.
  10. "Structural insights into the YAP and TEAD complex."
    Li Z., Zhao B., Wang P., Chen F., Dong Z., Yang H., Guan K.L., Xu Y.
    Genes Dev. 24:235-240(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 209-426 IN COMPLEX WITH YAP1, CHARACTERIZATION OF VARIANT SCRA HIS-421, MUTAGENESIS OF TYR-421.
  11. "A novel TEAD1 mutation is the causative allele in Sveinsson's chorioretinal atrophy (helicoid peripapillary chorioretinal degeneration)."
    Fossdal R., Jonasson F., Kristjansdottir G.T., Kong A., Stefansson H., Gosh S., Gulcher J.R., Stefansson K.
    Hum. Mol. Genet. 13:975-981(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANT SCRA HIS-421.

Entry informationi

Entry nameiTEAD1_HUMAN
AccessioniPrimary (citable) accession number: P28347
Secondary accession number(s): A4FUP2, E7EV65
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: July 15, 1998
Last modified: October 29, 2014
This is version 147 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3