P28347 (TEAD1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcriptional enhancer factor TEF-1

Alternative name(s):
NTEF-1
Protein GT-IIC
TEA domain family member 1
Short name=TEAD-1
Transcription factor 13
Short name=TCF-13

Gene names

Name:	TEAD1
Synonyms:	TCF13, TEF1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and cooperatively to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription in vivo in a cell-specific manner. The activation function appears to be mediated by a limiting cell-specific transcriptional intermediary factor (TIF). Involved in cardiac development. Binds to the M-CAT motif. Ref.4 Ref.5
Subunit structure	Interacts with YAP1 and WWTR1/TAZ. Ref.4 Ref.5
Subcellular location	Nucleus.
Tissue specificity	Preferentially expressed in skeletal muscle. Lower levels in pancreas, placenta, and heart.
Involvement in disease	Sveinsson chorioretinal atrophy (SCRA) [MIM:108985]: Characterized by symmetrical lesions radiating from the optic disk involving the retina and the choroid. Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.4 Ref.9 Ref.10
Sequence similarities	Contains 1 TEA DNA-binding domain.
Caution	It is uncertain whether Met-1 or Met-16 is the initiator.
Sequence caution	The sequence AAB00791.1 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical ATT isoleucine codon.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Disease	Disease mutation
Ligand	DNA-binding
Molecular function	Activator
PTM	Acetylation
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	hippo signaling cascade Inferred from direct assay Ref.4Ref.5. Source: UniProtKB transcription initiation from RNA polymerase II promoter Traceable author statement. Source: Reactome
Cellular_component	nucleoplasm Traceable author statement. Source: Reactome
Molecular_function	DNA binding Traceable author statement PubMed 8396764. Source: ProtInc sequence-specific DNA binding transcription factor activity Inferred from direct assay Ref.4Ref.5. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
vg	Q26366	3	EBI-529156,EBI-162687	From a different organism.
YAP1	P46937	2	EBI-529156,EBI-1044059

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 426

426

Transcriptional enhancer factor TEF-1

PRO_0000205930

Regions

DNA binding

30 – 97

68

TEA

Region

167 – 426

260

Transcriptional activation Potential

Compositional bias

143 – 204

62

Pro-rich

Compositional bias

306 – 328

23

Ser/Thr/Tyr-rich

Amino acid modifications

Modified residue

1

1

N-acetylmethionine Ref.6 Ref.7

Natural variations

Natural variant

421

1

Y → H in SCRA; loss of interaction with YAP1 and also activation by YAP1. Ref.4 Ref.9 Ref.10
Corresponds to variant rs11567847 [ dbSNP | Ensembl ].

VAR_031530

Experimental info

Mutagenesis

421

1

Y → A: Important loss of interaction with YAP1. Ref.9

Secondary structure

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426

Helix Strand Turn

Details...

Helix

29 – 31

3

Helix

37 – 49

13

Beta strand

52 – 54

3

Helix

60 – 63

4

Helix

70 – 81

12

Helix

88 – 101

14

Beta strand

216 – 230

15

Beta strand

233 – 242

10

Beta strand

256 – 258

3

Helix

259 – 261

3

Helix

263 – 265

3

Helix

273 – 279

7

Helix

282 – 284

3

Beta strand

285 – 292

8

Beta strand

303 – 316

14

Beta strand

319 – 328

10

Beta strand

331 – 341

11

Beta strand

343 – 345

3

Beta strand

348 – 357

10

Helix

360 – 370

11

Helix

375 – 382

8

Beta strand

385 – 393

9

Turn

394 – 396

3

Beta strand

399 – 409

11

Beta strand

412 – 414

3

Beta strand

417 – 424

8

Sequences

	Sequence		Length	Mass (Da)
	P28347 [UniParc]. Last modified July 15, 1998. Version 2. Checksum: EFD8067F2770B3C7	FASTA	426	47,946
	10 20 30 40 50 60 MEPSSWSGSE SPAENMERMS DSADKPIDND AEGVWSPDIE QSFQEALAIY PPCGRRKIIL 70 80 90 100 110 120 SDEGKMYGRN ELIARYIKLR TGKTRTRKQV SSHIQVLARR KSRDFHSKLK DQTAKDKALQ 130 140 150 160 170 180 HMAAMSSAQI VSATAIHNKL GLPGIPRPTF PGAPGFWPGM IQTGQPGSSQ DVKPFVQQAY 190 200 210 220 230 240 PIQPAVTAPI PGFEPASAPA PSVPAWQGRS IGTTKLRLVE FSAFLEQQRD PDSYNKHLFV 250 260 270 280 290 300 HIGHANHSYS DPLLESVDIR QIYDKFPEKK GGLKELFGKG PQNAFFLVKF WADLNCNIQD 310 320 330 340 350 360 DAGAFYGVTS QYESSENMTV TCSTKVCSFG KQVVEKVETE YARFENGRFV YRINRSPMCE 370 380 390 400 410 420 YMINFIHKLK HLPEKYMMNS VLENFTILLV VTNRDTQETL LCMACVFEVS NSEHGAQHHI YRLVKD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, expression, and transcriptional properties of the human enhancer factor TEF-1." Xiao J.H., Davidson I., Matthes H., Garnier J.-M., Chambon P. Cell 65:551-568(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The TEA domain: a novel, highly conserved DNA-binding motif." Buerglin T.R. Cell 66:11-12(1991) [PubMed] [Europe PMC] [Abstract] Cited for: DOMAIN TEA.
[4]	"TEAD mediates YAP-dependent gene induction and growth control." Zhao B., Ye X., Yu J., Li L., Li W., Li S., Yu J., Lin J.D., Wang C.Y., Chinnaiyan A.M., Lai Z.C., Guan K.L. Genes Dev. 22:1962-1971(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH YAP1, CHARACTERIZATION OF VARIANT SCRA HIS-421.
[5]	"TEAD transcription factors mediate the function of TAZ in cell growth and epithelial-mesenchymal transition." Zhang H., Liu C.Y., Zha Z.Y., Zhao B., Yao J., Zhao S., Xiong Y., Lei Q.Y., Guan K.L. J. Biol. Chem. 284:13355-13362(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, INTERACTION WITH WWTR1.
[6]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, MASS SPECTROMETRY.
[8]	"Insights into transcription enhancer factor 1 (TEF-1) activity from the solution structure of the TEA domain." Anbanandam A., Albarado D.C., Nguyen C.T., Halder G., Gao X., Veeraraghavan S. Proc. Natl. Acad. Sci. U.S.A. 103:17225-17230(2006) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 28-104.
[9]	"Structural insights into the YAP and TEAD complex." Li Z., Zhao B., Wang P., Chen F., Dong Z., Yang H., Guan K.L., Xu Y. Genes Dev. 24:235-240(2010) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 209-426 IN COMPLEX WITH YAP1, CHARACTERIZATION OF VARIANT SCRA HIS-421, MUTAGENESIS OF TYR-421.
[10]	"A novel TEAD1 mutation is the causative allele in Sveinsson's chorioretinal atrophy (helicoid peripapillary chorioretinal degeneration)." Fossdal R., Jonasson F., Kristjansdottir G.T., Kong A., Stefansson H., Gosh S., Gulcher J.R., Stefansson K. Hum. Mol. Genet. 13:975-981(2004) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT SCRA HIS-421.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M63896 Genomic DNA. Translation: AAB00791.1. Sequence problems.
AC013549 Genomic DNA. No translation available.
AC084859 Genomic DNA. No translation available.
AC107881 Genomic DNA. No translation available.

IPI

IPI00002901.

PIR

A40032.

RefSeq

NP_068780.2. NM_021961.5.

UniGene

Hs.655331.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2HZD	NMR	-	A	28-104	[»]
3KYS	X-ray	2.80	A/C	209-426	[»]

ProteinModelPortal

P28347.

ModBase

Search...

Protein-protein interaction databases

IntAct

P28347. 6 interactions.

STRING

9606.ENSP00000354588.

PTM databases

PhosphoSite

P28347.

Polymorphism databases

DMDM

3041733.

Proteomic databases

PaxDb

P28347.

PRIDE

P28347.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000361905; ENSP00000355332; ENSG00000187079.

GeneID

7003.

KEGG

hsa:7003.

UCSC

uc021qdx.1. human.

Organism-specific databases

CTD

7003.

GeneCards

GC11P012652.

H-InvDB

HIX0026218.

HGNC

HGNC:11714. TEAD1.

MIM

108985. phenotype.
189967. gene.

neXtProt

NX_P28347.

Orphanet

86813. Helicoid peripapillary chorioretinal degeneration.

PharmGKB

PA36432.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG246992.

HOGENOM

HOG000253933.

HOVERGEN

HBG056905.

InParanoid

P28347.

KO

K09448.

OrthoDB

EOG4QNMW5.

Enzyme and pathway databases

Reactome

REACT_71. Gene Expression.

Gene expression databases

ArrayExpress

P28347.

Bgee

P28347.

CleanEx

HS_TEAD1.

Genevestigator

P28347.

GermOnline

ENSG00000187079. Homo sapiens.

Family and domain databases

InterPro

IPR000818. TEA/ATTS.
IPR016361. TEF.
[Graphical view]

PANTHER

PTHR11834. PTHR11834. 1 hit.

Pfam

PF01285. TEA. 1 hit.
[Graphical view]

PIRSF

PIRSF002603. TEF. 1 hit.

PRINTS

PR00065. TEADOMAIN.

SMART

SM00426. TEA. 1 hit.
[Graphical view]

PROSITE

PS00554. TEA_1. 1 hit.
PS51088. TEA_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

TEAD1. human.

EvolutionaryTrace

P28347.

GenomeRNAi

7003.

NextBio

27350.

SOURCE

Search...

Entry information

Entry name

TEAD1_HUMAN

Accession

Primary (citable) accession number: P28347

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	July 15, 1998
Last modified:	May 1, 2013
This is version 132 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.