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P28347

- TEAD1_HUMAN

UniProt

P28347 - TEAD1_HUMAN

Protein

Transcriptional enhancer factor TEF-1

Gene

TEAD1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 146 (01 Oct 2014)
      Sequence version 2 (15 Jul 1998)
      Previous versions | rss
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    Functioni

    Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and cooperatively to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription in vivo in a cell-specific manner. The activation function appears to be mediated by a limiting cell-specific transcriptional intermediary factor (TIF). Involved in cardiac development. Binds to the M-CAT motif.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi30 – 9768TEAPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: ProtInc
    2. protein binding Source: UniProtKB
    3. sequence-specific DNA binding transcription factor activity Source: UniProtKB

    GO - Biological processi

    1. gene expression Source: Reactome
    2. hippo signaling Source: UniProtKB
    3. transcription initiation from RNA polymerase II promoter Source: Reactome

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_116145. PPARA activates gene expression.
    REACT_118713. YAP1- and WWTR1 (TAZ)-stimulated gene expression.
    SignaLinkiP28347.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcriptional enhancer factor TEF-1
    Alternative name(s):
    NTEF-1
    Protein GT-IIC
    TEA domain family member 1
    Short name:
    TEAD-1
    Transcription factor 13
    Short name:
    TCF-13
    Gene namesi
    Name:TEAD1
    Synonyms:TCF13, TEF1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:11714. TEAD1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: HPA
    2. Golgi apparatus Source: HPA
    3. nucleoplasm Source: Reactome
    4. nucleus Source: HPA

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Involvement in diseasei

    Sveinsson chorioretinal atrophy (SCRA) [MIM:108985]: Characterized by symmetrical lesions radiating from the optic disk involving the retina and the choroid.1 Publication
    Note: The disease is caused by mutations affecting the gene represented in this entry.
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti421 – 4211Y → H in SCRA; loss of interaction with YAP1 and also activation by YAP1. 1 Publication
    Corresponds to variant rs11567847 [ dbSNP | Ensembl ].
    VAR_031530

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi421 – 4211Y → A: Important loss of interaction with YAP1. 1 Publication

    Keywords - Diseasei

    Disease mutation

    Organism-specific databases

    MIMi108985. phenotype.
    Orphaneti86813. Helicoid peripapillary chorioretinal degeneration.
    PharmGKBiPA36432.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 426426Transcriptional enhancer factor TEF-1PRO_0000205930Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine2 Publications

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP28347.
    PaxDbiP28347.
    PRIDEiP28347.

    PTM databases

    PhosphoSiteiP28347.

    Expressioni

    Tissue specificityi

    Preferentially expressed in skeletal muscle. Lower levels in pancreas, placenta, and heart.

    Gene expression databases

    ArrayExpressiP28347.
    BgeeiP28347.
    CleanExiHS_TEAD1.
    GenevestigatoriP28347.

    Organism-specific databases

    HPAiHPA046053.

    Interactioni

    Subunit structurei

    Interacts with YAP1 and WWTR1/TAZ.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    vgQ263663EBI-529156,EBI-162687From a different organism.
    YAP1P469374EBI-529156,EBI-1044059

    Protein-protein interaction databases

    BioGridi112862. 13 interactions.
    IntActiP28347. 6 interactions.
    MINTiMINT-1512394.
    STRINGi9606.ENSP00000354588.

    Structurei

    Secondary structure

    1
    426
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi29 – 313
    Helixi37 – 4913
    Beta strandi52 – 543
    Helixi60 – 634
    Helixi70 – 8112
    Helixi88 – 10114
    Beta strandi216 – 23015
    Beta strandi233 – 24210
    Beta strandi256 – 2583
    Helixi259 – 2613
    Helixi263 – 2653
    Helixi273 – 2797
    Helixi282 – 2843
    Beta strandi285 – 2928
    Beta strandi303 – 31614
    Beta strandi319 – 32810
    Beta strandi331 – 34111
    Beta strandi343 – 3453
    Beta strandi348 – 35710
    Helixi360 – 37011
    Helixi375 – 3828
    Beta strandi385 – 3939
    Turni394 – 3963
    Beta strandi399 – 40911
    Beta strandi412 – 4143
    Beta strandi417 – 4248

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2HZDNMR-A28-104[»]
    3KYSX-ray2.80A/C209-426[»]
    ProteinModelPortaliP28347.
    SMRiP28347. Positions 27-105, 209-425.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP28347.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni167 – 426260Transcriptional activationSequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi143 – 20462Pro-richAdd
    BLAST
    Compositional biasi306 – 32823Ser/Thr/Tyr-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 TEA DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG246992.
    HOGENOMiHOG000253933.
    HOVERGENiHBG056905.
    InParanoidiP28347.
    KOiK09448.
    OrthoDBiEOG7288RP.
    PhylomeDBiP28347.

    Family and domain databases

    InterProiIPR000818. TEA/ATTS.
    IPR016361. TEF.
    [Graphical view]
    PANTHERiPTHR11834. PTHR11834. 1 hit.
    PfamiPF01285. TEA. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002603. TEF. 1 hit.
    PRINTSiPR00065. TEADOMAIN.
    SMARTiSM00426. TEA. 1 hit.
    [Graphical view]
    PROSITEiPS00554. TEA_1. 1 hit.
    PS51088. TEA_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P28347-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEPSSWSGSE SPAENMERMS DSADKPIDND AEGVWSPDIE QSFQEALAIY    50
    PPCGRRKIIL SDEGKMYGRN ELIARYIKLR TGKTRTRKQV SSHIQVLARR 100
    KSRDFHSKLK DQTAKDKALQ HMAAMSSAQI VSATAIHNKL GLPGIPRPTF 150
    PGAPGFWPGM IQTGQPGSSQ DVKPFVQQAY PIQPAVTAPI PGFEPASAPA 200
    PSVPAWQGRS IGTTKLRLVE FSAFLEQQRD PDSYNKHLFV HIGHANHSYS 250
    DPLLESVDIR QIYDKFPEKK GGLKELFGKG PQNAFFLVKF WADLNCNIQD 300
    DAGAFYGVTS QYESSENMTV TCSTKVCSFG KQVVEKVETE YARFENGRFV 350
    YRINRSPMCE YMINFIHKLK HLPEKYMMNS VLENFTILLV VTNRDTQETL 400
    LCMACVFEVS NSEHGAQHHI YRLVKD 426
    Length:426
    Mass (Da):47,946
    Last modified:July 15, 1998 - v2
    Checksum:iEFD8067F2770B3C7
    GO
    Isoform 2 (identifier: P28347-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-15: Missing.
         110-110: K → KVTSM
         234-291: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:357
    Mass (Da):40,000
    Checksum:i0CCACF105ABDFFD5
    GO

    Sequence cautioni

    The sequence AAB00791.1 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical ATT isoleucine codon.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti421 – 4211Y → H in SCRA; loss of interaction with YAP1 and also activation by YAP1. 1 Publication
    Corresponds to variant rs11567847 [ dbSNP | Ensembl ].
    VAR_031530

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 1515Missing in isoform 2. 1 PublicationVSP_056275Add
    BLAST
    Alternative sequencei110 – 1101K → KVTSM in isoform 2. 1 PublicationVSP_056276
    Alternative sequencei234 – 29158Missing in isoform 2. 1 PublicationVSP_056277Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M63896 Genomic DNA. Translation: AAB00791.1. Sequence problems.
    AC013549 Genomic DNA. No translation available.
    AC084859 Genomic DNA. No translation available.
    AC107881 Genomic DNA. No translation available.
    BC115398 mRNA. Translation: AAI15399.1.
    CCDSiCCDS7810.2.
    PIRiA40032.
    RefSeqiNP_068780.2. NM_021961.5.
    UniGeneiHs.655331.

    Genome annotation databases

    EnsembliENST00000361905; ENSP00000355332; ENSG00000187079.
    GeneIDi7003.
    KEGGihsa:7003.
    UCSCiuc021qdx.1. human.

    Polymorphism databases

    DMDMi3041733.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M63896 Genomic DNA. Translation: AAB00791.1 . Sequence problems.
    AC013549 Genomic DNA. No translation available.
    AC084859 Genomic DNA. No translation available.
    AC107881 Genomic DNA. No translation available.
    BC115398 mRNA. Translation: AAI15399.1 .
    CCDSi CCDS7810.2.
    PIRi A40032.
    RefSeqi NP_068780.2. NM_021961.5.
    UniGenei Hs.655331.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2HZD NMR - A 28-104 [» ]
    3KYS X-ray 2.80 A/C 209-426 [» ]
    ProteinModelPortali P28347.
    SMRi P28347. Positions 27-105, 209-425.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 112862. 13 interactions.
    IntActi P28347. 6 interactions.
    MINTi MINT-1512394.
    STRINGi 9606.ENSP00000354588.

    PTM databases

    PhosphoSitei P28347.

    Polymorphism databases

    DMDMi 3041733.

    Proteomic databases

    MaxQBi P28347.
    PaxDbi P28347.
    PRIDEi P28347.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000361905 ; ENSP00000355332 ; ENSG00000187079 .
    GeneIDi 7003.
    KEGGi hsa:7003.
    UCSCi uc021qdx.1. human.

    Organism-specific databases

    CTDi 7003.
    GeneCardsi GC11P012652.
    H-InvDB HIX0026218.
    HGNCi HGNC:11714. TEAD1.
    HPAi HPA046053.
    MIMi 108985. phenotype.
    189967. gene.
    neXtProti NX_P28347.
    Orphaneti 86813. Helicoid peripapillary chorioretinal degeneration.
    PharmGKBi PA36432.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG246992.
    HOGENOMi HOG000253933.
    HOVERGENi HBG056905.
    InParanoidi P28347.
    KOi K09448.
    OrthoDBi EOG7288RP.
    PhylomeDBi P28347.

    Enzyme and pathway databases

    Reactomei REACT_116145. PPARA activates gene expression.
    REACT_118713. YAP1- and WWTR1 (TAZ)-stimulated gene expression.
    SignaLinki P28347.

    Miscellaneous databases

    ChiTaRSi TEAD1. human.
    EvolutionaryTracei P28347.
    GeneWikii TEAD1.
    GenomeRNAii 7003.
    NextBioi 27350.
    PROi P28347.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P28347.
    Bgeei P28347.
    CleanExi HS_TEAD1.
    Genevestigatori P28347.

    Family and domain databases

    InterProi IPR000818. TEA/ATTS.
    IPR016361. TEF.
    [Graphical view ]
    PANTHERi PTHR11834. PTHR11834. 1 hit.
    Pfami PF01285. TEA. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002603. TEF. 1 hit.
    PRINTSi PR00065. TEADOMAIN.
    SMARTi SM00426. TEA. 1 hit.
    [Graphical view ]
    PROSITEi PS00554. TEA_1. 1 hit.
    PS51088. TEA_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, expression, and transcriptional properties of the human enhancer factor TEF-1."
      Xiao J.H., Davidson I., Matthes H., Garnier J.-M., Chambon P.
      Cell 65:551-568(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    4. "The TEA domain: a novel, highly conserved DNA-binding motif."
      Buerglin T.R.
      Cell 66:11-12(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: DOMAIN TEA.
    5. "TEAD mediates YAP-dependent gene induction and growth control."
      Zhao B., Ye X., Yu J., Li L., Li W., Li S., Yu J., Lin J.D., Wang C.Y., Chinnaiyan A.M., Lai Z.C., Guan K.L.
      Genes Dev. 22:1962-1971(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH YAP1, CHARACTERIZATION OF VARIANT SCRA HIS-421.
    6. "TEAD transcription factors mediate the function of TAZ in cell growth and epithelial-mesenchymal transition."
      Zhang H., Liu C.Y., Zha Z.Y., Zhao B., Yao J., Zhao S., Xiong Y., Lei Q.Y., Guan K.L.
      J. Biol. Chem. 284:13355-13362(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, INTERACTION WITH WWTR1.
    7. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    8. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Insights into transcription enhancer factor 1 (TEF-1) activity from the solution structure of the TEA domain."
      Anbanandam A., Albarado D.C., Nguyen C.T., Halder G., Gao X., Veeraraghavan S.
      Proc. Natl. Acad. Sci. U.S.A. 103:17225-17230(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 28-104.
    10. "Structural insights into the YAP and TEAD complex."
      Li Z., Zhao B., Wang P., Chen F., Dong Z., Yang H., Guan K.L., Xu Y.
      Genes Dev. 24:235-240(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 209-426 IN COMPLEX WITH YAP1, CHARACTERIZATION OF VARIANT SCRA HIS-421, MUTAGENESIS OF TYR-421.
    11. "A novel TEAD1 mutation is the causative allele in Sveinsson's chorioretinal atrophy (helicoid peripapillary chorioretinal degeneration)."
      Fossdal R., Jonasson F., Kristjansdottir G.T., Kong A., Stefansson H., Gosh S., Gulcher J.R., Stefansson K.
      Hum. Mol. Genet. 13:975-981(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANT SCRA HIS-421.

    Entry informationi

    Entry nameiTEAD1_HUMAN
    AccessioniPrimary (citable) accession number: P28347
    Secondary accession number(s): A4FUP2, E7EV65
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: July 15, 1998
    Last modified: October 1, 2014
    This is version 146 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3