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P28345 (MASY_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate synthase, glyoxysomal

EC=2.3.3.9
Alternative name(s):
Acetate utilization protein 9
Gene names
Name:acu-9
ORF Names:NCU10007
OrganismNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) [Reference proteome]
Taxonomic identifier367110 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora

Protein attributes

Sequence length542 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.

Pathway

Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.

Subcellular location

Glyoxysome.

Sequence similarities

Belongs to the malate synthase family.

Ontologies

Keywords
   Biological processGlyoxylate bypass
Tricarboxylic acid cycle
   Cellular componentGlyoxysome
Peroxisome
   Molecular functionTransferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglyoxylate cycle

Inferred from electronic annotation. Source: UniProtKB-UniPathway

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentglyoxysome

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmalate synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 542542Malate synthase, glyoxysomal
PRO_0000166862

Regions

Motif540 – 5423Microbody targeting signal Potential

Sites

Active site1681Proton acceptor By similarity
Active site4491Proton donor By similarity

Experimental info

Sequence conflict52 – 532QL → HV in CAA39994. Ref.1
Sequence conflict83 – 875PPAPG → APAAPP Ref.1
Sequence conflict951I → M in CAA39994. Ref.1
Sequence conflict2151L → P in CAA39994. Ref.1
Sequence conflict242 – 2432RG → LS in CAA39994. Ref.1
Sequence conflict2551L → T in CAA39994. Ref.1
Sequence conflict2651Y → F in CAA39994. Ref.1
Sequence conflict269 – 2713EHS → NHT in CAA39994. Ref.1
Sequence conflict276 – 2783CGR → RGG in CAA39994. Ref.1
Sequence conflict284 – 2852ST → PF in CAA39994. Ref.1
Sequence conflict288 – 2947KFRNHSS → EVRRFPN in CAA39994. Ref.1
Sequence conflict3021C → D in CAA39994. Ref.1
Sequence conflict3111D → E in CAA39994. Ref.1
Sequence conflict319 – 3257QTCHKRG → KTLHRLV in CAA39994. Ref.1
Sequence conflict3311Missing in CAA39994. Ref.1
Sequence conflict4151I → S in CAA39994. Ref.1
Sequence conflict4181E → D in CAA39994. Ref.1
Sequence conflict4261I → T in CAA39994. Ref.1
Sequence conflict431 – 4333MEA → TEP in CAA39994. Ref.1
Sequence conflict444 – 4474NYLM → KHPQ in CAA39994. Ref.1
Sequence conflict4671G → R in CAA39994. Ref.1
Sequence conflict4751R → H in CAA39994. Ref.1
Sequence conflict479 – 4813SYA → RYP in CAA39994. Ref.1
Sequence conflict488 – 4947QTDELAS → ADRQRLA in CAA39994. Ref.1
Sequence conflict5111T → S in CAA39994. Ref.1
Sequence conflict5241S → C in CAA39994. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P28345 [UniParc].

Last modified April 13, 2004. Version 2.
Checksum: EE0F085DD30DAC20

FASTA54261,180
        10         20         30         40         50         60 
MASVETLLQG VTISGPIEEH QRKILTPQAL SFVALLHRSF NQTRKNLLER RQLRQAEIDR 

        70         80         90        100        110        120 
GVLPDFLPET KHIRENPTWK GAPPAPGLVD RRVEITGPTD RKMVVNALNS DVYTYMADFE 

       130        140        150        160        170        180 
DSSAPTWANM VNGQVNLYDA IRRQIDFKQG PKEYKLRTDR TLPTLIVRPR GWHLEEKHVT 

       190        200        210        220        230        240 
IDGEPVSGSL FDFGLYFFHN AKELVQRGFG PYFYLPKMES HLEARLWNDA FNLAQDYVGI 

       250        260        270        280        290        300 
PRGTIRGTVL IETILAAFEM DEIIYELREH SSGLNCGRWD YIFSTIKKFR NHSSFVLPDR 

       310        320        330        340        350        360 
SCVTMTVPFM DAYVKLLIQT CHKRGVHAMG GMAAQIPIKD DKAANDKAME GVRADKLREA 

       370        380        390        400        410        420 
RAGHDGTWVA HPALASIALE VFNKHMPTPN QLFNRREDVK IGQQDLLNMN VPGSITEEGI 

       430        440        450        460        470        480 
RKNLNIGLGY MEAWIRGVGC VPINYLMEDA ATAEVSRSQL WQWVKHGVTT AEGKRVDKSY 

       490        500        510        520        530        540 
ALKLLKEQTD ELASKAPQGN KFNLAAQYFA TQVTGEDYAD FLTSLLYNEI TSAGNSLPAS 


KL 

« Hide

References

« Hide 'large scale' references
[1]"Molecular organisation of the malate synthase genes of Aspergillus nidulans and Neurospora crassa."
Sandeman R.A., Hynes M.J., Fincham J.R.S., Connerton I.F.
Mol. Gen. Genet. 228:445-452(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The genome sequence of the filamentous fungus Neurospora crassa."
Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. expand/collapse author list , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X56672 Genomic DNA. Translation: CAA39994.1.
CM002236 Genomic DNA. Translation: ESA43774.1.
CM002236 Genomic DNA. Translation: ESA43775.1.
PIRS17774.
RefSeqXP_959453.1. XM_954360.2.
UniGeneNcr.14021.

3D structure databases

ProteinModelPortalP28345.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5141.NCU10007.1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiEFNCRT00000008728; EFNCRP00000008710; EFNCRG00000008714.
GeneID3875600.
KEGGncr:NCU10007.

Phylogenomic databases

eggNOGCOG2225.
HOGENOMHOG000238464.
KOK01638.
OMASIWQWIR.
OrthoDBEOG7GXPMK.

Enzyme and pathway databases

UniPathwayUPA00703; UER00720.

Family and domain databases

InterProIPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view]
PfamPF01274. Malate_synthase. 1 hit.
[Graphical view]
PIRSFPIRSF001363. Malate_synth. 1 hit.
SUPFAMSSF51645. SSF51645. 1 hit.
TIGRFAMsTIGR01344. malate_syn_A. 1 hit.
PROSITEPS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMASY_NEUCR
AccessionPrimary (citable) accession number: P28345
Secondary accession number(s): Q7RV95, V5IPX4
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: April 13, 2004
Last modified: June 11, 2014
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways