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Protein

Malate synthase, glyoxysomal

Gene

acu-9

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.

Pathwayi: glyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Isocitrate lyase (acu-3)
  2. Malate synthase, glyoxysomal (acu-9)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei168Proton acceptorBy similarity1
Active sitei449Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase, glyoxysomal (EC:2.3.3.9)
Alternative name(s):
Acetate utilization protein 9
Gene namesi
Name:acu-9
ORF Names:NCU10007
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
Proteomesi
  • UP000001805 Componenti: Chromosome 1, Linkage Group I

Organism-specific databases

EuPathDBiFungiDB:NCU10007.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Glyoxysome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001668621 – 542Malate synthase, glyoxysomalAdd BLAST542

Proteomic databases

PRIDEiP28345.

Structurei

3D structure databases

ProteinModelPortaliP28345.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi540 – 542Microbody targeting signalSequence analysis3

Sequence similaritiesi

Belongs to the malate synthase family.Curated

Phylogenomic databases

HOGENOMiHOG000238464.
InParanoidiP28345.
KOiK01638.
OMAiCHKRGAP.
OrthoDBiEOG092C1NQD.

Family and domain databases

CDDicd00727. malate_synt_A. 1 hit.
InterProiIPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001363. Malate_synth. 1 hit.
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01344. malate_syn_A. 1 hit.
PROSITEiPS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28345-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASVETLLQG VTISGPIEEH QRKILTPQAL SFVALLHRSF NQTRKNLLER
60 70 80 90 100
RQLRQAEIDR GVLPDFLPET KHIRENPTWK GAPPAPGLVD RRVEITGPTD
110 120 130 140 150
RKMVVNALNS DVYTYMADFE DSSAPTWANM VNGQVNLYDA IRRQIDFKQG
160 170 180 190 200
PKEYKLRTDR TLPTLIVRPR GWHLEEKHVT IDGEPVSGSL FDFGLYFFHN
210 220 230 240 250
AKELVQRGFG PYFYLPKMES HLEARLWNDA FNLAQDYVGI PRGTIRGTVL
260 270 280 290 300
IETILAAFEM DEIIYELREH SSGLNCGRWD YIFSTIKKFR NHSSFVLPDR
310 320 330 340 350
SCVTMTVPFM DAYVKLLIQT CHKRGVHAMG GMAAQIPIKD DKAANDKAME
360 370 380 390 400
GVRADKLREA RAGHDGTWVA HPALASIALE VFNKHMPTPN QLFNRREDVK
410 420 430 440 450
IGQQDLLNMN VPGSITEEGI RKNLNIGLGY MEAWIRGVGC VPINYLMEDA
460 470 480 490 500
ATAEVSRSQL WQWVKHGVTT AEGKRVDKSY ALKLLKEQTD ELASKAPQGN
510 520 530 540
KFNLAAQYFA TQVTGEDYAD FLTSLLYNEI TSAGNSLPAS KL
Length:542
Mass (Da):61,180
Last modified:April 13, 2004 - v2
Checksum:iEE0F085DD30DAC20
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti52 – 53QL → HV in CAA39994 (PubMed:1832736).Curated2
Sequence conflicti83 – 87PPAPG → APAAPP (PubMed:1832736).Curated5
Sequence conflicti95I → M in CAA39994 (PubMed:1832736).Curated1
Sequence conflicti215L → P in CAA39994 (PubMed:1832736).Curated1
Sequence conflicti242 – 243RG → LS in CAA39994 (PubMed:1832736).Curated2
Sequence conflicti255L → T in CAA39994 (PubMed:1832736).Curated1
Sequence conflicti265Y → F in CAA39994 (PubMed:1832736).Curated1
Sequence conflicti269 – 271EHS → NHT in CAA39994 (PubMed:1832736).Curated3
Sequence conflicti276 – 278CGR → RGG in CAA39994 (PubMed:1832736).Curated3
Sequence conflicti284 – 285ST → PF in CAA39994 (PubMed:1832736).Curated2
Sequence conflicti288 – 294KFRNHSS → EVRRFPN in CAA39994 (PubMed:1832736).Curated7
Sequence conflicti302C → D in CAA39994 (PubMed:1832736).Curated1
Sequence conflicti311D → E in CAA39994 (PubMed:1832736).Curated1
Sequence conflicti319 – 325QTCHKRG → KTLHRLV in CAA39994 (PubMed:1832736).Curated7
Sequence conflicti331Missing in CAA39994 (PubMed:1832736).Curated1
Sequence conflicti415I → S in CAA39994 (PubMed:1832736).Curated1
Sequence conflicti418E → D in CAA39994 (PubMed:1832736).Curated1
Sequence conflicti426I → T in CAA39994 (PubMed:1832736).Curated1
Sequence conflicti431 – 433MEA → TEP in CAA39994 (PubMed:1832736).Curated3
Sequence conflicti444 – 447NYLM → KHPQ in CAA39994 (PubMed:1832736).Curated4
Sequence conflicti467G → R in CAA39994 (PubMed:1832736).Curated1
Sequence conflicti475R → H in CAA39994 (PubMed:1832736).Curated1
Sequence conflicti479 – 481SYA → RYP in CAA39994 (PubMed:1832736).Curated3
Sequence conflicti488 – 494QTDELAS → ADRQRLA in CAA39994 (PubMed:1832736).Curated7
Sequence conflicti511T → S in CAA39994 (PubMed:1832736).Curated1
Sequence conflicti524S → C in CAA39994 (PubMed:1832736).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56672 Genomic DNA. Translation: CAA39994.1.
CM002236 Genomic DNA. Translation: ESA43774.1.
CM002236 Genomic DNA. Translation: ESA43775.1.
PIRiS17774.
RefSeqiXP_011393298.1. XM_011394996.1.
XP_011393299.1. XM_011394997.1.

Genome annotation databases

EnsemblFungiiESA43774; ESA43774; NCU10007.
ESA43775; ESA43775; NCU10007.
GeneIDi3875600.
KEGGincr:NCU10007.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56672 Genomic DNA. Translation: CAA39994.1.
CM002236 Genomic DNA. Translation: ESA43774.1.
CM002236 Genomic DNA. Translation: ESA43775.1.
PIRiS17774.
RefSeqiXP_011393298.1. XM_011394996.1.
XP_011393299.1. XM_011394997.1.

3D structure databases

ProteinModelPortaliP28345.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP28345.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiESA43774; ESA43774; NCU10007.
ESA43775; ESA43775; NCU10007.
GeneIDi3875600.
KEGGincr:NCU10007.

Organism-specific databases

EuPathDBiFungiDB:NCU10007.

Phylogenomic databases

HOGENOMiHOG000238464.
InParanoidiP28345.
KOiK01638.
OMAiCHKRGAP.
OrthoDBiEOG092C1NQD.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Family and domain databases

CDDicd00727. malate_synt_A. 1 hit.
InterProiIPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001363. Malate_synth. 1 hit.
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01344. malate_syn_A. 1 hit.
PROSITEiPS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMASY_NEUCR
AccessioniPrimary (citable) accession number: P28345
Secondary accession number(s): Q7RV95, V5IPX4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: April 13, 2004
Last modified: November 30, 2016
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.