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P28345

- MASY_NEUCR

UniProt

P28345 - MASY_NEUCR

Protein

Malate synthase, glyoxysomal

Gene

acu-9

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 2 (13 Apr 2004)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei168 – 1681Proton acceptorBy similarity
    Active sitei449 – 4491Proton donorBy similarity

    GO - Molecular functioni

    1. malate synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-UniPathway
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Enzyme and pathway databases

    UniPathwayiUPA00703; UER00720.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate synthase, glyoxysomal (EC:2.3.3.9)
    Alternative name(s):
    Acetate utilization protein 9
    Gene namesi
    Name:acu-9
    ORF Names:NCU10007
    OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
    Taxonomic identifieri367110 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
    ProteomesiUP000001805: Chromosome 1, Linkage Group I

    Subcellular locationi

    GO - Cellular componenti

    1. glyoxysome Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Glyoxysome, Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 542542Malate synthase, glyoxysomalPRO_0000166862Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi5141.NCU10007.1.

    Structurei

    3D structure databases

    ProteinModelPortaliP28345.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi540 – 5423Microbody targeting signalSequence Analysis

    Sequence similaritiesi

    Belongs to the malate synthase family.Curated

    Phylogenomic databases

    eggNOGiCOG2225.
    HOGENOMiHOG000238464.
    KOiK01638.
    OMAiSIWQWIR.
    OrthoDBiEOG7GXPMK.

    Family and domain databases

    InterProiIPR011076. Malate_synth-like.
    IPR006252. Malate_synthA.
    IPR001465. Malate_synthase.
    IPR019830. Malate_synthase_CS.
    [Graphical view]
    PfamiPF01274. Malate_synthase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001363. Malate_synth. 1 hit.
    SUPFAMiSSF51645. SSF51645. 1 hit.
    TIGRFAMsiTIGR01344. malate_syn_A. 1 hit.
    PROSITEiPS00510. MALATE_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P28345-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASVETLLQG VTISGPIEEH QRKILTPQAL SFVALLHRSF NQTRKNLLER    50
    RQLRQAEIDR GVLPDFLPET KHIRENPTWK GAPPAPGLVD RRVEITGPTD 100
    RKMVVNALNS DVYTYMADFE DSSAPTWANM VNGQVNLYDA IRRQIDFKQG 150
    PKEYKLRTDR TLPTLIVRPR GWHLEEKHVT IDGEPVSGSL FDFGLYFFHN 200
    AKELVQRGFG PYFYLPKMES HLEARLWNDA FNLAQDYVGI PRGTIRGTVL 250
    IETILAAFEM DEIIYELREH SSGLNCGRWD YIFSTIKKFR NHSSFVLPDR 300
    SCVTMTVPFM DAYVKLLIQT CHKRGVHAMG GMAAQIPIKD DKAANDKAME 350
    GVRADKLREA RAGHDGTWVA HPALASIALE VFNKHMPTPN QLFNRREDVK 400
    IGQQDLLNMN VPGSITEEGI RKNLNIGLGY MEAWIRGVGC VPINYLMEDA 450
    ATAEVSRSQL WQWVKHGVTT AEGKRVDKSY ALKLLKEQTD ELASKAPQGN 500
    KFNLAAQYFA TQVTGEDYAD FLTSLLYNEI TSAGNSLPAS KL 542
    Length:542
    Mass (Da):61,180
    Last modified:April 13, 2004 - v2
    Checksum:iEE0F085DD30DAC20
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti52 – 532QL → HV in CAA39994. (PubMed:1832736)Curated
    Sequence conflicti83 – 875PPAPG → APAAPP(PubMed:1832736)Curated
    Sequence conflicti95 – 951I → M in CAA39994. (PubMed:1832736)Curated
    Sequence conflicti215 – 2151L → P in CAA39994. (PubMed:1832736)Curated
    Sequence conflicti242 – 2432RG → LS in CAA39994. (PubMed:1832736)Curated
    Sequence conflicti255 – 2551L → T in CAA39994. (PubMed:1832736)Curated
    Sequence conflicti265 – 2651Y → F in CAA39994. (PubMed:1832736)Curated
    Sequence conflicti269 – 2713EHS → NHT in CAA39994. (PubMed:1832736)Curated
    Sequence conflicti276 – 2783CGR → RGG in CAA39994. (PubMed:1832736)Curated
    Sequence conflicti284 – 2852ST → PF in CAA39994. (PubMed:1832736)Curated
    Sequence conflicti288 – 2947KFRNHSS → EVRRFPN in CAA39994. (PubMed:1832736)Curated
    Sequence conflicti302 – 3021C → D in CAA39994. (PubMed:1832736)Curated
    Sequence conflicti311 – 3111D → E in CAA39994. (PubMed:1832736)Curated
    Sequence conflicti319 – 3257QTCHKRG → KTLHRLV in CAA39994. (PubMed:1832736)Curated
    Sequence conflicti331 – 3311Missing in CAA39994. (PubMed:1832736)Curated
    Sequence conflicti415 – 4151I → S in CAA39994. (PubMed:1832736)Curated
    Sequence conflicti418 – 4181E → D in CAA39994. (PubMed:1832736)Curated
    Sequence conflicti426 – 4261I → T in CAA39994. (PubMed:1832736)Curated
    Sequence conflicti431 – 4333MEA → TEP in CAA39994. (PubMed:1832736)Curated
    Sequence conflicti444 – 4474NYLM → KHPQ in CAA39994. (PubMed:1832736)Curated
    Sequence conflicti467 – 4671G → R in CAA39994. (PubMed:1832736)Curated
    Sequence conflicti475 – 4751R → H in CAA39994. (PubMed:1832736)Curated
    Sequence conflicti479 – 4813SYA → RYP in CAA39994. (PubMed:1832736)Curated
    Sequence conflicti488 – 4947QTDELAS → ADRQRLA in CAA39994. (PubMed:1832736)Curated
    Sequence conflicti511 – 5111T → S in CAA39994. (PubMed:1832736)Curated
    Sequence conflicti524 – 5241S → C in CAA39994. (PubMed:1832736)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X56672 Genomic DNA. Translation: CAA39994.1.
    CM002236 Genomic DNA. Translation: ESA43774.1.
    CM002236 Genomic DNA. Translation: ESA43775.1.
    PIRiS17774.
    RefSeqiXP_959453.1. XM_954360.2.
    UniGeneiNcr.14021.

    Genome annotation databases

    EnsemblFungiiEFNCRT00000008728; EFNCRP00000008710; EFNCRG00000008714.
    GeneIDi3875600.
    KEGGincr:NCU10007.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X56672 Genomic DNA. Translation: CAA39994.1 .
    CM002236 Genomic DNA. Translation: ESA43774.1 .
    CM002236 Genomic DNA. Translation: ESA43775.1 .
    PIRi S17774.
    RefSeqi XP_959453.1. XM_954360.2.
    UniGenei Ncr.14021.

    3D structure databases

    ProteinModelPortali P28345.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5141.NCU10007.1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii EFNCRT00000008728 ; EFNCRP00000008710 ; EFNCRG00000008714 .
    GeneIDi 3875600.
    KEGGi ncr:NCU10007.

    Phylogenomic databases

    eggNOGi COG2225.
    HOGENOMi HOG000238464.
    KOi K01638.
    OMAi SIWQWIR.
    OrthoDBi EOG7GXPMK.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00720 .

    Family and domain databases

    InterProi IPR011076. Malate_synth-like.
    IPR006252. Malate_synthA.
    IPR001465. Malate_synthase.
    IPR019830. Malate_synthase_CS.
    [Graphical view ]
    Pfami PF01274. Malate_synthase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001363. Malate_synth. 1 hit.
    SUPFAMi SSF51645. SSF51645. 1 hit.
    TIGRFAMsi TIGR01344. malate_syn_A. 1 hit.
    PROSITEi PS00510. MALATE_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular organisation of the malate synthase genes of Aspergillus nidulans and Neurospora crassa."
      Sandeman R.A., Hynes M.J., Fincham J.R.S., Connerton I.F.
      Mol. Gen. Genet. 228:445-452(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The genome sequence of the filamentous fungus Neurospora crassa."
      Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
      , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
      Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

    Entry informationi

    Entry nameiMASY_NEUCR
    AccessioniPrimary (citable) accession number: P28345
    Secondary accession number(s): Q7RV95, V5IPX4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: April 13, 2004
    Last modified: October 1, 2014
    This is version 108 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3