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P28345

- MASY_NEUCR

UniProt

P28345 - MASY_NEUCR

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Protein

Malate synthase, glyoxysomal

Gene

acu-9

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei168 – 1681Proton acceptorBy similarity
Active sitei449 – 4491Proton donorBy similarity

GO - Molecular functioni

  1. malate synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-UniPathway
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase, glyoxysomal (EC:2.3.3.9)
Alternative name(s):
Acetate utilization protein 9
Gene namesi
Name:acu-9
ORF Names:NCU10007
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
ProteomesiUP000001805: Chromosome 1, Linkage Group I

Subcellular locationi

GO - Cellular componenti

  1. glyoxysome Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Glyoxysome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 542542Malate synthase, glyoxysomalPRO_0000166862Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi5141.NCU10007.1.

Structurei

3D structure databases

ProteinModelPortaliP28345.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi540 – 5423Microbody targeting signalSequence Analysis

Sequence similaritiesi

Belongs to the malate synthase family.Curated

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000238464.
InParanoidiP28345.
KOiK01638.
OMAiSIWQWIR.
OrthoDBiEOG7GXPMK.

Family and domain databases

InterProiIPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001363. Malate_synth. 1 hit.
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01344. malate_syn_A. 1 hit.
PROSITEiPS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28345-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASVETLLQG VTISGPIEEH QRKILTPQAL SFVALLHRSF NQTRKNLLER
60 70 80 90 100
RQLRQAEIDR GVLPDFLPET KHIRENPTWK GAPPAPGLVD RRVEITGPTD
110 120 130 140 150
RKMVVNALNS DVYTYMADFE DSSAPTWANM VNGQVNLYDA IRRQIDFKQG
160 170 180 190 200
PKEYKLRTDR TLPTLIVRPR GWHLEEKHVT IDGEPVSGSL FDFGLYFFHN
210 220 230 240 250
AKELVQRGFG PYFYLPKMES HLEARLWNDA FNLAQDYVGI PRGTIRGTVL
260 270 280 290 300
IETILAAFEM DEIIYELREH SSGLNCGRWD YIFSTIKKFR NHSSFVLPDR
310 320 330 340 350
SCVTMTVPFM DAYVKLLIQT CHKRGVHAMG GMAAQIPIKD DKAANDKAME
360 370 380 390 400
GVRADKLREA RAGHDGTWVA HPALASIALE VFNKHMPTPN QLFNRREDVK
410 420 430 440 450
IGQQDLLNMN VPGSITEEGI RKNLNIGLGY MEAWIRGVGC VPINYLMEDA
460 470 480 490 500
ATAEVSRSQL WQWVKHGVTT AEGKRVDKSY ALKLLKEQTD ELASKAPQGN
510 520 530 540
KFNLAAQYFA TQVTGEDYAD FLTSLLYNEI TSAGNSLPAS KL
Length:542
Mass (Da):61,180
Last modified:April 13, 2004 - v2
Checksum:iEE0F085DD30DAC20
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti52 – 532QL → HV in CAA39994. (PubMed:1832736)Curated
Sequence conflicti83 – 875PPAPG → APAAPP(PubMed:1832736)Curated
Sequence conflicti95 – 951I → M in CAA39994. (PubMed:1832736)Curated
Sequence conflicti215 – 2151L → P in CAA39994. (PubMed:1832736)Curated
Sequence conflicti242 – 2432RG → LS in CAA39994. (PubMed:1832736)Curated
Sequence conflicti255 – 2551L → T in CAA39994. (PubMed:1832736)Curated
Sequence conflicti265 – 2651Y → F in CAA39994. (PubMed:1832736)Curated
Sequence conflicti269 – 2713EHS → NHT in CAA39994. (PubMed:1832736)Curated
Sequence conflicti276 – 2783CGR → RGG in CAA39994. (PubMed:1832736)Curated
Sequence conflicti284 – 2852ST → PF in CAA39994. (PubMed:1832736)Curated
Sequence conflicti288 – 2947KFRNHSS → EVRRFPN in CAA39994. (PubMed:1832736)Curated
Sequence conflicti302 – 3021C → D in CAA39994. (PubMed:1832736)Curated
Sequence conflicti311 – 3111D → E in CAA39994. (PubMed:1832736)Curated
Sequence conflicti319 – 3257QTCHKRG → KTLHRLV in CAA39994. (PubMed:1832736)Curated
Sequence conflicti331 – 3311Missing in CAA39994. (PubMed:1832736)Curated
Sequence conflicti415 – 4151I → S in CAA39994. (PubMed:1832736)Curated
Sequence conflicti418 – 4181E → D in CAA39994. (PubMed:1832736)Curated
Sequence conflicti426 – 4261I → T in CAA39994. (PubMed:1832736)Curated
Sequence conflicti431 – 4333MEA → TEP in CAA39994. (PubMed:1832736)Curated
Sequence conflicti444 – 4474NYLM → KHPQ in CAA39994. (PubMed:1832736)Curated
Sequence conflicti467 – 4671G → R in CAA39994. (PubMed:1832736)Curated
Sequence conflicti475 – 4751R → H in CAA39994. (PubMed:1832736)Curated
Sequence conflicti479 – 4813SYA → RYP in CAA39994. (PubMed:1832736)Curated
Sequence conflicti488 – 4947QTDELAS → ADRQRLA in CAA39994. (PubMed:1832736)Curated
Sequence conflicti511 – 5111T → S in CAA39994. (PubMed:1832736)Curated
Sequence conflicti524 – 5241S → C in CAA39994. (PubMed:1832736)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X56672 Genomic DNA. Translation: CAA39994.1.
CM002236 Genomic DNA. Translation: ESA43774.1.
CM002236 Genomic DNA. Translation: ESA43775.1.
PIRiS17774.
RefSeqiXP_959453.1. XM_954360.2.
UniGeneiNcr.14021.

Genome annotation databases

EnsemblFungiiEFNCRT00000008728; EFNCRP00000008710; EFNCRG00000008714.
GeneIDi3875600.
KEGGincr:NCU10007.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X56672 Genomic DNA. Translation: CAA39994.1 .
CM002236 Genomic DNA. Translation: ESA43774.1 .
CM002236 Genomic DNA. Translation: ESA43775.1 .
PIRi S17774.
RefSeqi XP_959453.1. XM_954360.2.
UniGenei Ncr.14021.

3D structure databases

ProteinModelPortali P28345.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5141.NCU10007.1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii EFNCRT00000008728 ; EFNCRP00000008710 ; EFNCRG00000008714 .
GeneIDi 3875600.
KEGGi ncr:NCU10007.

Phylogenomic databases

eggNOGi COG2225.
HOGENOMi HOG000238464.
InParanoidi P28345.
KOi K01638.
OMAi SIWQWIR.
OrthoDBi EOG7GXPMK.

Enzyme and pathway databases

UniPathwayi UPA00703 ; UER00720 .

Family and domain databases

InterProi IPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view ]
Pfami PF01274. Malate_synthase. 1 hit.
[Graphical view ]
PIRSFi PIRSF001363. Malate_synth. 1 hit.
SUPFAMi SSF51645. SSF51645. 1 hit.
TIGRFAMsi TIGR01344. malate_syn_A. 1 hit.
PROSITEi PS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular organisation of the malate synthase genes of Aspergillus nidulans and Neurospora crassa."
    Sandeman R.A., Hynes M.J., Fincham J.R.S., Connerton I.F.
    Mol. Gen. Genet. 228:445-452(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The genome sequence of the filamentous fungus Neurospora crassa."
    Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
    , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
    Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Entry informationi

Entry nameiMASY_NEUCR
AccessioniPrimary (citable) accession number: P28345
Secondary accession number(s): Q7RV95, V5IPX4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: April 13, 2004
Last modified: October 29, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3