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P28344 (MASY_EMENI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate synthase, glyoxysomal

EC=2.3.3.9
Gene names
Name:acuE
ORF Names:AN6653
OrganismEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) [Reference proteome]
Taxonomic identifier227321 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Protein attributes

Sequence length539 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.

Pathway

Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.

Subcellular location

Glyoxysome.

Sequence similarities

Belongs to the malate synthase family.

Sequence caution

The sequence CAA39993.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence EAA58182.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 539539Malate synthase, glyoxysomal
PRO_0000166861

Regions

Motif537 – 5393Microbody targeting signal Potential

Sites

Active site1681Proton acceptor By similarity
Active site4491Proton donor By similarity

Experimental info

Sequence conflict5101A → S in CAA39993. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P28344 [UniParc].

Last modified November 16, 2011. Version 3.
Checksum: D29334EA211735F8

FASTA53960,797
        10         20         30         40         50         60 
MSQVDAQLKD VAILGSVSNE ARKILTKEAC AFLAILHRTF NPTRKALLQR RVDRQAEIDK 

        70         80         90        100        110        120 
GHLPDFLPET KHIRDDPSWK GAPPAPGLVD RRVEITGPTD RKMVVNALNS DVWTYMADFE 

       130        140        150        160        170        180 
DSSAPTWDNM INGQINLYDA IRRQVDFKQG QKEYKLRTDR TLPTLIARAR GWHLDEKHFT 

       190        200        210        220        230        240 
VDGEPISGSL FDFGLYFFHN AKELVARGFG PYFYLPKMES HLEARLWNDV FNLAQDYIGM 

       250        260        270        280        290        300 
PRGTIRGTVL IETITAAFEM EEIIYELRDH SSGLNCGRWD YIFSFIKKFR QHPNFVLPDR 

       310        320        330        340        350        360 
SDVTMTVPFM DAYVKLLIKT CHKRGVHAMG GMAAQIPIKD NAEANDKAME GVRADKLREV 

       370        380        390        400        410        420 
RAGHDGTWVA HPALASIASE VFNKYMPTPN QMHVRREDVN ITANDLLNTN VPGKITEDGI 

       430        440        450        460        470        480 
RKNLNIGLSY MEGWLRGVGC IPINYLMEDA ATAEVSRSQL WQWARHGVTT SEGKKVDKAY 

       490        500        510        520        530 
ALRLLKEQAD ALAAKGPKGN KFQLAGRYFA GQVTGEDYAD FLTSLLYNEI SSPGTASKL 

« Hide

References

« Hide 'large scale' references
[1]"Molecular organisation of the malate synthase genes of Aspergillus nidulans and Neurospora crassa."
Sandeman R.A., Hynes M.J., Fincham J.R.S., Connerton I.F.
Mol. Gen. Genet. 228:445-452(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae."
Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S., Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V., Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S., Braus G.H. expand/collapse author list , Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H., Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K., Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R., Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C., Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L., Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W.
Nature 438:1105-1115(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
[3]"The 2008 update of the Aspergillus nidulans genome annotation: a community effort."
Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G. expand/collapse author list , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G.
Fungal Genet. Biol. 46:S2-13(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X56671 Genomic DNA. Translation: CAA39993.1. Sequence problems.
AACD01000110 Genomic DNA. Translation: EAA58182.1. Sequence problems.
BN001301 Genomic DNA. Translation: CBF71180.1.
PIRS17773.
RefSeqXP_664257.1. XM_659165.1.

3D structure databases

ProteinModelPortalP28344.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING162425.CADANIAP00007436.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiCADANIAT00007436; CADANIAP00007436; CADANIAG00007436.
GeneID2870422.
KEGGani:AN6653.2.

Phylogenomic databases

eggNOGCOG2225.
HOGENOMHOG000238464.
KOK01638.
OMAREGDWAC.
OrthoDBEOG7GXPMK.

Enzyme and pathway databases

UniPathwayUPA00703; UER00720.

Family and domain databases

InterProIPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view]
PfamPF01274. Malate_synthase. 1 hit.
[Graphical view]
PIRSFPIRSF001363. Malate_synth. 1 hit.
SUPFAMSSF51645. SSF51645. 1 hit.
TIGRFAMsTIGR01344. malate_syn_A. 1 hit.
PROSITEPS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMASY_EMENI
AccessionPrimary (citable) accession number: P28344
Secondary accession number(s): C8V1E0, Q5AYH7
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: November 16, 2011
Last modified: June 11, 2014
This is version 92 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways