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P28344

- MASY_EMENI

UniProt

P28344 - MASY_EMENI

Protein

Malate synthase, glyoxysomal

Gene

acuE

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 3 (16 Nov 2011)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei168 – 1681Proton acceptorBy similarity
    Active sitei449 – 4491Proton donorBy similarity

    GO - Molecular functioni

    1. malate synthase activity Source: ASPGD

    GO - Biological processi

    1. acetate catabolic process Source: ASPGD
    2. carbon utilization Source: ASPGD
    3. fatty acid catabolic process Source: ASPGD
    4. glyoxylate cycle Source: ASPGD
    5. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Enzyme and pathway databases

    UniPathwayiUPA00703; UER00720.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate synthase, glyoxysomal (EC:2.3.3.9)
    Gene namesi
    Name:acuE
    ORF Names:AN6653
    OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
    Taxonomic identifieri227321 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    ProteomesiUP000000560: Chromosome I

    Subcellular locationi

    GO - Cellular componenti

    1. glyoxysome Source: UniProtKB-SubCell
    2. peroxisomal matrix Source: ASPGD
    3. peroxisome Source: ASPGD

    Keywords - Cellular componenti

    Glyoxysome, Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 539539Malate synthase, glyoxysomalPRO_0000166861Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi162425.CADANIAP00007436.

    Structurei

    3D structure databases

    ProteinModelPortaliP28344.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi537 – 5393Microbody targeting signalSequence Analysis

    Sequence similaritiesi

    Belongs to the malate synthase family.Curated

    Phylogenomic databases

    eggNOGiCOG2225.
    HOGENOMiHOG000238464.
    KOiK01638.
    OMAiREGDWAC.
    OrthoDBiEOG7GXPMK.

    Family and domain databases

    InterProiIPR011076. Malate_synth-like.
    IPR006252. Malate_synthA.
    IPR001465. Malate_synthase.
    IPR019830. Malate_synthase_CS.
    [Graphical view]
    PfamiPF01274. Malate_synthase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001363. Malate_synth. 1 hit.
    SUPFAMiSSF51645. SSF51645. 1 hit.
    TIGRFAMsiTIGR01344. malate_syn_A. 1 hit.
    PROSITEiPS00510. MALATE_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P28344-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQVDAQLKD VAILGSVSNE ARKILTKEAC AFLAILHRTF NPTRKALLQR    50
    RVDRQAEIDK GHLPDFLPET KHIRDDPSWK GAPPAPGLVD RRVEITGPTD 100
    RKMVVNALNS DVWTYMADFE DSSAPTWDNM INGQINLYDA IRRQVDFKQG 150
    QKEYKLRTDR TLPTLIARAR GWHLDEKHFT VDGEPISGSL FDFGLYFFHN 200
    AKELVARGFG PYFYLPKMES HLEARLWNDV FNLAQDYIGM PRGTIRGTVL 250
    IETITAAFEM EEIIYELRDH SSGLNCGRWD YIFSFIKKFR QHPNFVLPDR 300
    SDVTMTVPFM DAYVKLLIKT CHKRGVHAMG GMAAQIPIKD NAEANDKAME 350
    GVRADKLREV RAGHDGTWVA HPALASIASE VFNKYMPTPN QMHVRREDVN 400
    ITANDLLNTN VPGKITEDGI RKNLNIGLSY MEGWLRGVGC IPINYLMEDA 450
    ATAEVSRSQL WQWARHGVTT SEGKKVDKAY ALRLLKEQAD ALAAKGPKGN 500
    KFQLAGRYFA GQVTGEDYAD FLTSLLYNEI SSPGTASKL 539
    Length:539
    Mass (Da):60,797
    Last modified:November 16, 2011 - v3
    Checksum:iD29334EA211735F8
    GO

    Sequence cautioni

    The sequence CAA39993.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence EAA58182.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti510 – 5101A → S in CAA39993. (PubMed:1832736)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X56671 Genomic DNA. Translation: CAA39993.1. Sequence problems.
    AACD01000110 Genomic DNA. Translation: EAA58182.1. Sequence problems.
    BN001301 Genomic DNA. Translation: CBF71180.1.
    PIRiS17773.
    RefSeqiXP_664257.1. XM_659165.1.

    Genome annotation databases

    EnsemblFungiiCADANIAT00007436; CADANIAP00007436; CADANIAG00007436.
    GeneIDi2870422.
    KEGGiani:AN6653.2.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X56671 Genomic DNA. Translation: CAA39993.1 . Sequence problems.
    AACD01000110 Genomic DNA. Translation: EAA58182.1 . Sequence problems.
    BN001301 Genomic DNA. Translation: CBF71180.1 .
    PIRi S17773.
    RefSeqi XP_664257.1. XM_659165.1.

    3D structure databases

    ProteinModelPortali P28344.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 162425.CADANIAP00007436.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii CADANIAT00007436 ; CADANIAP00007436 ; CADANIAG00007436 .
    GeneIDi 2870422.
    KEGGi ani:AN6653.2.

    Phylogenomic databases

    eggNOGi COG2225.
    HOGENOMi HOG000238464.
    KOi K01638.
    OMAi REGDWAC.
    OrthoDBi EOG7GXPMK.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00720 .

    Family and domain databases

    InterProi IPR011076. Malate_synth-like.
    IPR006252. Malate_synthA.
    IPR001465. Malate_synthase.
    IPR019830. Malate_synthase_CS.
    [Graphical view ]
    Pfami PF01274. Malate_synthase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001363. Malate_synth. 1 hit.
    SUPFAMi SSF51645. SSF51645. 1 hit.
    TIGRFAMsi TIGR01344. malate_syn_A. 1 hit.
    PROSITEi PS00510. MALATE_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular organisation of the malate synthase genes of Aspergillus nidulans and Neurospora crassa."
      Sandeman R.A., Hynes M.J., Fincham J.R.S., Connerton I.F.
      Mol. Gen. Genet. 228:445-452(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
    3. "The 2008 update of the Aspergillus nidulans genome annotation: a community effort."
      Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G.
      , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G.
      Fungal Genet. Biol. 46:S2-13(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.

    Entry informationi

    Entry nameiMASY_EMENI
    AccessioniPrimary (citable) accession number: P28344
    Secondary accession number(s): C8V1E0, Q5AYH7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: November 16, 2011
    Last modified: October 1, 2014
    This is version 93 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3