P28344 (MASY_EMENI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate synthase, glyoxysomal
EC=2.3.3.9

Gene names

Name:	acuE
ORF Names:	AN6653

Organism

Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) [Reference proteome]

Taxonomic identifier

227321 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › Emericella › Emericella nidulans › mitosporic Emericella nidulans

Protein attributes

Sequence length	539 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.
Subcellular location	Glyoxysome.
Sequence similarities	Belongs to the malate synthase family.
Sequence caution	The sequence CAA39993.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence EAA58182.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Glyoxysome Peroxisome
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-UniPathway tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	glyoxysome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	malate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 539	539	Malate synthase, glyoxysomal		PRO_0000166861
Regions
	Motif	537 – 539	3	Microbody targeting signal Potential
Sites
	Active site	168	1	Proton acceptor By similarity
	Active site	449	1	Proton donor By similarity
Experimental info
	Sequence conflict	510	1	A → S in CAA39993. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P28344 [UniParc]. Last modified November 16, 2011. Version 3. Checksum: D29334EA211735F8	FASTA	539	60,797
	10 20 30 40 50 60 MSQVDAQLKD VAILGSVSNE ARKILTKEAC AFLAILHRTF NPTRKALLQR RVDRQAEIDK 70 80 90 100 110 120 GHLPDFLPET KHIRDDPSWK GAPPAPGLVD RRVEITGPTD RKMVVNALNS DVWTYMADFE 130 140 150 160 170 180 DSSAPTWDNM INGQINLYDA IRRQVDFKQG QKEYKLRTDR TLPTLIARAR GWHLDEKHFT 190 200 210 220 230 240 VDGEPISGSL FDFGLYFFHN AKELVARGFG PYFYLPKMES HLEARLWNDV FNLAQDYIGM 250 260 270 280 290 300 PRGTIRGTVL IETITAAFEM EEIIYELRDH SSGLNCGRWD YIFSFIKKFR QHPNFVLPDR 310 320 330 340 350 360 SDVTMTVPFM DAYVKLLIKT CHKRGVHAMG GMAAQIPIKD NAEANDKAME GVRADKLREV 370 380 390 400 410 420 RAGHDGTWVA HPALASIASE VFNKYMPTPN QMHVRREDVN ITANDLLNTN VPGKITEDGI 430 440 450 460 470 480 RKNLNIGLSY MEGWLRGVGC IPINYLMEDA ATAEVSRSQL WQWARHGVTT SEGKKVDKAY 490 500 510 520 530 ALRLLKEQAD ALAAKGPKGN KFQLAGRYFA GQVTGEDYAD FLTSLLYNEI SSPGTASKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular organisation of the malate synthase genes of Aspergillus nidulans and Neurospora crassa." Sandeman R.A., Hynes M.J., Fincham J.R.S., Connerton I.F. Mol. Gen. Genet. 228:445-452(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae." Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S., Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V., Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S., Braus G.H. , Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H., Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K., Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R., Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C., Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L., Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W. Nature 438:1105-1115(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
[3]	"The 2008 update of the Aspergillus nidulans genome annotation: a community effort." Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G. , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G. Fungal Genet. Biol. 46:S2-13(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X56671 Genomic DNA. Translation: CAA39993.1. Sequence problems. AACD01000110 Genomic DNA. Translation: EAA58182.1. Sequence problems. BN001301 Genomic DNA. Translation: CBF71180.1.
PIR	S17773.
RefSeq	XP_664257.1. XM_659165.1.
3D structure databases
ProteinModelPortal	P28344.
ModBase	Search...
Protein-protein interaction databases
STRING	162425.CADANIAP00007436.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2870422.
KEGG	ani:AN6653.2.
Phylogenomic databases
eggNOG	COG2225.
HOGENOM	HOG000238464.
KO	K01638.
OMA	GFDGTWV.
OrthoDB	EOG4TXG13.
Enzyme and pathway databases
UniPathway	UPA00703; UER00720.
Family and domain databases
InterPro	IPR011076. Malate_synth-like. IPR006252. Malate_synthA. IPR001465. Malate_synthase. IPR019830. Malate_synthase_CS. [Graphical view]
PANTHER	PTHR21631:SF1. PTHR21631:SF1. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF001363. Malate_synth. 1 hit.
SUPFAM	SSF51645. Malat_synth_like. 1 hit.
TIGRFAMs	TIGR01344. malate_syn_A. 1 hit.
PROSITE	PS00510. MALATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MASY_EMENI

Accession

Primary (citable) accession number: P28344
Secondary accession number(s): C8V1E0, Q5AYH7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	November 16, 2011
Last modified:	April 3, 2013
This is version 85 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program