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Protein

Malate synthase, glyoxysomal

Gene

acuE

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei168 – 1681Proton acceptorBy similarity
Active sitei449 – 4491Proton donorBy similarity

GO - Molecular functioni

  1. malate synthase activity Source: ASPGD

GO - Biological processi

  1. acetate catabolic process Source: ASPGD
  2. carbon utilization Source: ASPGD
  3. fatty acid catabolic process Source: ASPGD
  4. glyoxylate cycle Source: ASPGD
  5. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase, glyoxysomal (EC:2.3.3.9)
Gene namesi
Name:acuE
ORF Names:AN6653
OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic identifieri227321 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000000560: Chromosome I

Subcellular locationi

GO - Cellular componenti

  1. glyoxysome Source: UniProtKB-SubCell
  2. peroxisomal matrix Source: ASPGD
  3. peroxisome Source: ASPGD
Complete GO annotation...

Keywords - Cellular componenti

Glyoxysome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 539539Malate synthase, glyoxysomalPRO_0000166861Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi162425.CADANIAP00007436.

Structurei

3D structure databases

ProteinModelPortaliP28344.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi537 – 5393Microbody targeting signalSequence Analysis

Sequence similaritiesi

Belongs to the malate synthase family.Curated

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000238464.
InParanoidiP28344.
KOiK01638.
OMAiMMINALN.
OrthoDBiEOG7GXPMK.

Family and domain databases

InterProiIPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001363. Malate_synth. 1 hit.
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01344. malate_syn_A. 1 hit.
PROSITEiPS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28344-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSQVDAQLKD VAILGSVSNE ARKILTKEAC AFLAILHRTF NPTRKALLQR
60 70 80 90 100
RVDRQAEIDK GHLPDFLPET KHIRDDPSWK GAPPAPGLVD RRVEITGPTD
110 120 130 140 150
RKMVVNALNS DVWTYMADFE DSSAPTWDNM INGQINLYDA IRRQVDFKQG
160 170 180 190 200
QKEYKLRTDR TLPTLIARAR GWHLDEKHFT VDGEPISGSL FDFGLYFFHN
210 220 230 240 250
AKELVARGFG PYFYLPKMES HLEARLWNDV FNLAQDYIGM PRGTIRGTVL
260 270 280 290 300
IETITAAFEM EEIIYELRDH SSGLNCGRWD YIFSFIKKFR QHPNFVLPDR
310 320 330 340 350
SDVTMTVPFM DAYVKLLIKT CHKRGVHAMG GMAAQIPIKD NAEANDKAME
360 370 380 390 400
GVRADKLREV RAGHDGTWVA HPALASIASE VFNKYMPTPN QMHVRREDVN
410 420 430 440 450
ITANDLLNTN VPGKITEDGI RKNLNIGLSY MEGWLRGVGC IPINYLMEDA
460 470 480 490 500
ATAEVSRSQL WQWARHGVTT SEGKKVDKAY ALRLLKEQAD ALAAKGPKGN
510 520 530
KFQLAGRYFA GQVTGEDYAD FLTSLLYNEI SSPGTASKL
Length:539
Mass (Da):60,797
Last modified:November 16, 2011 - v3
Checksum:iD29334EA211735F8
GO

Sequence cautioni

The sequence CAA39993.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence EAA58182.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti510 – 5101A → S in CAA39993. (PubMed:1832736)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56671 Genomic DNA. Translation: CAA39993.1. Sequence problems.
AACD01000110 Genomic DNA. Translation: EAA58182.1. Sequence problems.
BN001301 Genomic DNA. Translation: CBF71180.1.
PIRiS17773.
RefSeqiXP_664257.1. XM_659165.1.

Genome annotation databases

EnsemblFungiiCADANIAT00007436; CADANIAP00007436; CADANIAG00007436.
GeneIDi2870422.
KEGGiani:AN6653.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56671 Genomic DNA. Translation: CAA39993.1. Sequence problems.
AACD01000110 Genomic DNA. Translation: EAA58182.1. Sequence problems.
BN001301 Genomic DNA. Translation: CBF71180.1.
PIRiS17773.
RefSeqiXP_664257.1. XM_659165.1.

3D structure databases

ProteinModelPortaliP28344.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi162425.CADANIAP00007436.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADANIAT00007436; CADANIAP00007436; CADANIAG00007436.
GeneIDi2870422.
KEGGiani:AN6653.2.

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000238464.
InParanoidiP28344.
KOiK01638.
OMAiMMINALN.
OrthoDBiEOG7GXPMK.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Family and domain databases

InterProiIPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001363. Malate_synth. 1 hit.
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01344. malate_syn_A. 1 hit.
PROSITEiPS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular organisation of the malate synthase genes of Aspergillus nidulans and Neurospora crassa."
    Sandeman R.A., Hynes M.J., Fincham J.R.S., Connerton I.F.
    Mol. Gen. Genet. 228:445-452(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
  3. "The 2008 update of the Aspergillus nidulans genome annotation: a community effort."
    Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G.
    , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G.
    Fungal Genet. Biol. 46:S2-13(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.

Entry informationi

Entry nameiMASY_EMENI
AccessioniPrimary (citable) accession number: P28344
Secondary accession number(s): C8V1E0, Q5AYH7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: November 16, 2011
Last modified: January 7, 2015
This is version 95 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.