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P28343

- THS1_VITVI

UniProt

P28343 - THS1_VITVI

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Protein

Stilbene synthase 1

Gene
VINST1, STS2, VST1, GSVIVT00009226001, LOC100256566, VITISV_035301
Organism
Vitis vinifera (Grape)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Mediates resistance to pathogens which are sensitive to stilbenes such as Botrytis cinerea, Eutypa lata and Plasmopora viticola by enhancing the production of phytoalexins. Confers resistance to Phytophthora palmivora when expressed in papaya.3 Publications

Catalytic activityi

3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + trans-resveratrol + 4 CO2.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei164 – 1641 By similarity
Binding sitei267 – 2671Substrate; via carbonyl oxygen By similarity

GO - Molecular functioni

  1. trihydroxystilbene synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. biosynthetic process Source: InterPro
  2. defense response Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Plant defense, Stress response

Enzyme and pathway databases

UniPathwayiUPA00372; UER00548.

Names & Taxonomyi

Protein namesi
Recommended name:
Stilbene synthase 1 (EC:2.3.1.95)
Alternative name(s):
PSV25
Resveratrol synthase 1
Trihydroxystilbene synthase 1
Short name:
StSy 1
Vitis stilbene synthase 1
Gene namesi
Name:VINST1
Synonyms:STS2, VST1
ORF Names:GSVIVT00009226001, LOC100256566, VITISV_035301
OrganismiVitis vinifera (Grape)
Taxonomic identifieri29760 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsVitalesVitaceaeVitis

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Biotechnological usei

Driven by a stress-inducible promoter, stilbene synthase is used to improve resistance to pathogens which are sensitive to stilbenes such as B.cinerea, P.palmivora, E.lata or P.viticola.6 Publications
Expression in heterologous systems (e.g. S.cerevisiae and Populus alba) is used to produce resveratrol for human food and medication. Resveratrol, a product of the chemical reaction catalized by stilbene synthase, exhibits several clinical benefits for human diseases, such as cancers, cardiovascular diseases and has positive effects on longevity.6 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 392392Stilbene synthase 1PRO_0000216082Add
BLAST

Expressioni

Tissue specificityi

In leaves, expressed in palisade and spongy parenchyma cells and, to a lesser extent, in epidermal cells after induction.1 Publication

Inductioni

By stress, including UV light, ozone and pathogens such as B.cinerea. Strongly induced by ergosterol, a non-specific fungal elicitor. Positively regulated by jasmonate (JA) and methyl-jasmonate (MeJA). Induction by phytopathogen attack decreases with grape berry ripening.6 Publications

Interactioni

Subunit structurei

Homodimer By similarity.

Structurei

3D structure databases

ProteinModelPortaliP28343.
SMRiP28343. Positions 3-387.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni55 – 584Substrate binding By similarity
Regioni305 – 3073Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3424.
HOGENOMiHOG000245460.
KOiK00660.

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR012328. Chalcone/stilbene_synth_C.
IPR018088. Chalcone/stilbene_synthase_AS.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000451. PKS_III. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
PROSITEiPS00441. CHALCONE_SYNTH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28343-1 [UniParc]FASTAAdd to Basket

« Hide

MASVEEFRNA QRAKGPATIL AIGTATPDHC VYQSDYADYY FRVTKSEHMT    50
ELKKKFNRIC DKSMIKKRYI HLTEEMLEEH PNIGAYMAPS LNIRQEIITA 100
EVPRLGRDAA LKALKEWGQP KSKITHLVFC TTSGVEMPGA DYKLANLLGL 150
ETSVRRVMLY HQGCYAGGTV LRTAKDLAEN NAGARVLVVC SEITVVTFRG 200
PSEDALDSLV GQALFGDGSS AVIVGSDPDV SIERPLFQLV SAAQTFIPNS 250
AGAIAGNLRE VGLTFHLWPN VPTLISENIE KCLTQAFDPL GISDWNSLFW 300
IAHPGGPAIL DAVEAKLNLE KKKLEATRHV LSEYGNMSSA CVLFILDEMR 350
KKSLKGEKAT TGEGLDWGVL FGFGPGLTIE TVVLHSVPTV TN 392
Length:392
Mass (Da):42,840
Last modified:October 1, 1996 - v2
Checksum:i0C2E8C2553792431
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti387 – 3893VPT → IPM in S63227. 1 Publication
Sequence conflicti387 – 3893VPT → IPM in ABC84860. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S63225 mRNA. No translation available.
S63227 mRNA. No translation available.
AB046375 Genomic DNA. Translation: BAB20980.1.
DQ366302 mRNA. Translation: ABC84860.1.
AM426171 Genomic DNA. Translation: CAN60215.1.
PIRiS11044.
RefSeqiNP_001267939.1. NM_001281010.1.
UniGeneiVvi.27488.
Vvi.8.

Genome annotation databases

GeneIDi100256566.
KEGGivvi:100256566.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S63225 mRNA. No translation available.
S63227 mRNA. No translation available.
AB046375 Genomic DNA. Translation: BAB20980.1 .
DQ366302 mRNA. Translation: ABC84860.1 .
AM426171 Genomic DNA. Translation: CAN60215.1 .
PIRi S11044.
RefSeqi NP_001267939.1. NM_001281010.1.
UniGenei Vvi.27488.
Vvi.8.

3D structure databases

ProteinModelPortali P28343.
SMRi P28343. Positions 3-387.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100256566.
KEGGi vvi:100256566.

Phylogenomic databases

eggNOGi COG3424.
HOGENOMi HOG000245460.
KOi K00660.

Enzyme and pathway databases

UniPathwayi UPA00372 ; UER00548 .

Family and domain databases

Gene3Di 3.40.47.10. 2 hits.
InterProi IPR012328. Chalcone/stilbene_synth_C.
IPR018088. Chalcone/stilbene_synthase_AS.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view ]
Pfami PF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000451. PKS_III. 1 hit.
SUPFAMi SSF53901. SSF53901. 2 hits.
PROSITEi PS00441. CHALCONE_SYNTH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Grapevine stilbene synthase cDNA only slightly differing from chalcone synthase cDNA is expressed in Escherichia coli into a catalytically active enzyme."
    Melchior F., Kindl H.
    FEBS Lett. 268:17-20(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Optima.
  2. "Coordinate- and elicitor-dependent expression of stilbene synthase and phenylalanine ammonia-lyase genes in Vitis cv. Optima."
    Melchior F., Kindl H.
    Arch. Biochem. Biophys. 288:552-557(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Optima.
  3. "Kiwifruits (Actinidia deliciosa) transformed with a Vitis stilbene synthase gene produce piceid (resveratrol-glucoside)."
    Kobayashi S., Ding C., Nakamura Y., Nakajima I., Matsumoto R.
    Plant Cell Rep. 19:904-910(2000)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Optima.
  4. Zhang Y., Yu O.
    Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  5. "The grapevine genome sequence suggests ancestral hexaploidization in major angiosperm phyla."
    Jaillon O., Aury J.-M., Noel B., Policriti A., Clepet C., Casagrande A., Choisne N., Aubourg S., Vitulo N., Jubin C., Vezzi A., Legeai F., Hugueney P., Dasilva C., Horner D., Mica E., Jublot D., Poulain J.
    , Bruyere C., Billault A., Segurens B., Gouyvenoux M., Ugarte E., Cattonaro F., Anthouard V., Vico V., Del Fabbro C., Alaux M., Di Gaspero G., Dumas V., Felice N., Paillard S., Juman I., Moroldo M., Scalabrin S., Canaguier A., Le Clainche I., Malacrida G., Durand E., Pesole G., Laucou V., Chatelet P., Merdinoglu D., Delledonne M., Pezzotti M., Lecharny A., Scarpelli C., Artiguenave F., Pe M.E., Valle G., Morgante M., Caboche M., Adam-Blondon A.-F., Weissenbach J., Quetier F., Wincker P.
    Nature 449:463-467(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Pinot noir / PN40024.
  6. "A high quality draft consensus sequence of the genome of a heterozygous grapevine variety."
    Velasco R., Zharkikh A., Troggio M., Cartwright D.A., Cestaro A., Pruss D., Pindo M., FitzGerald L.M., Vezzulli S., Reid J., Malacarne G., Iliev D., Coppola G., Wardell B., Micheletti D., Macalma T., Facci M., Mitchell J.T.
    , Perazzolli M., Eldredge G., Gatto P., Oyzerski R., Moretto M., Gutin N., Stefanini M., Chen Y., Segala C., Davenport C., Dematte L., Mraz A., Battilana J., Stormo K., Costa F., Tao Q., Si-Ammour A., Harkins T., Lackey A., Perbost C., Taillon B., Stella A., Solovyev V., Fawcett J.A., Sterck L., Vandepoele K., Grando S.M., Toppo S., Moser C., Lanchbury J., Bogden R., Skolnick M., Sgaramella V., Bhatnagar S.K., Fontana P., Gutin A., Van de Peer Y., Salamini F., Viola R.
    PLoS ONE 2:E1326-E1326(2007)
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Pinot noir.
  7. "An ozone-responsive region of the grapevine resveratrol synthase promoter differs from the basal pathogen-responsive sequence."
    Schubert R., Fischer R., Hain R., Schreier P.H., Bahnweg G., Ernst D., Sandermann H. Jr.
    Plant Mol. Biol. 34:417-426(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY OZONE AND PATHOGENS, TISSUE SPECIFICITY.
  8. "In vitro tolerance to Botrytis cinerea of grapevine 41B rootstock in transgenic plants expressing the stilbene synthase Vst1 gene under the control of a pathogen-inducible PR 10 promoter."
    Coutos-Thevenot P., Poinssot B., Bonomelli A., Yean H., Breda C., Buffard D., Esnault R., Hain R., Boulay M.
    J. Exp. Bot. 52:901-910(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOTECHNOLOGY, INDUCTION BY PHYTOPATHOGEN AND ABIOTIC STRESS.
  9. "Nucleic acid comprising the sequence of a promoter unductible by stress and a gene sequence coding for a stilbene synthase."
    Coutos-Thevenot P., Hain R., Schreier P.-H., Boulay M., Esnault R.
    Patent number US6800794, 05-OCT-2004
    Cited for: FUNCTION, BIOTECHNOLOGY.
  10. "Expression of the grapevine stilbene synthase gene VST1 in papaya provides increased resistance against diseases caused by Phytophthora palmivora."
    Zhu Y.J., Agbayani R., Jackson M.C., Tang C.S., Moore P.H.
    Planta 220:241-250(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOTECHNOLOGY.
  11. "Expression of the stilbene synthase (StSy) gene from grapevine in transgenic white poplar results in high accumulation of the antioxidant resveratrol glucosides."
    Giorcelli A., Sparvoli F., Mattivi F., Tava A., Balestrazzi A., Vrhovsek U., Calligari P., Bollini R., Confalonieri M.
    Transgenic Res. 13:203-214(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOTECHNOLOGY.
  12. "Jasmonates and Na-orthovanadate promote resveratrol production in Vitis vinifera cv. Barbera cell cultures."
    Tassoni A., Fornale S., Franceschetti M., Musiani F., Michael A.J., Perry B., Bagni N.
    New Phytol. 166:895-905(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INDUCTION BY JA.
  13. "Antioxidant metabolite profiles in tomato fruit constitutively expressing the grapevine stilbene synthase gene."
    Giovinazzo G., D'Amico L., Paradiso A., Bollini R., Sparvoli F., DeGara L.
    Plant Biotechnol. J. 3:57-69(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOTECHNOLOGY.
  14. "Methyl jasmonate induces defense responses in grapevine and triggers protection against Erysiphe necator."
    Belhadj A., Saigne C., Telef N., Cluzet S., Bouscaut J., Corio-Costet M.-F., Merillon J.-M.
    J. Agric. Food Chem. 54:9119-9125(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY JA.
  15. "Transgenic peas (Pisum sativum) expressing polygalacturonase inhibiting protein from raspberry (Rubus idaeus) and stilbene synthase from grape (Vitis vinifera)."
    Richter A., de Kathen A., de Lorenzo G., Briviba K., Hain R., Ramsay G., Jacobsen H.-J., Kiesecker H.
    Plant Cell Rep. 25:1166-1173(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY UV LIGHT.
  16. Cited for: BIOTECHNOLOGY.
  17. "Molecular basis of ergosterol-induced protection of grape against botrytis cinerea: induction of type I LTP promoter activity, WRKY, and stilbene synthase gene expression."
    Laquitaine L., Gomes E., Francois J., Marchive C., Pascal S., Hamdi S., Atanassova R., Delrot S., Coutos-Thevenot P.
    Mol. Plant Microbe Interact. 19:1103-1112(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY ERGOSTEROL.
  18. "Chemoprevention by resveratrol: molecular mechanisms and therapeutic potential."
    Shankar S., Singh G., Srivastava R.K.
    Front. Biosci. 12:4839-4854(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiTHS1_VITVI
AccessioniPrimary (citable) accession number: P28343
Secondary accession number(s): A5AGH1, Q2HY09, Q54AD0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Genetically modified food

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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