Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Stilbene synthase 1

Gene

VINST1

Organism
Vitis vinifera (Grape)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Mediates resistance to pathogens which are sensitive to stilbenes such as Botrytis cinerea, Eutypa lata and Plasmopora viticola by enhancing the production of phytoalexins. Confers resistance to Phytophthora palmivora when expressed in papaya.3 Publications

Catalytic activityi

3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + trans-resveratrol + 4 CO2.

Pathwayi: 3,4',5-trihydroxystilbene biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes 3,4',5-trihydroxystilbene from trans-4-coumarate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Stilbene synthase 1 (VINST1), Stilbene synthase 6 (STS), Stilbene synthase 2 (GSVIVT00004047001), Stilbene synthase 4 (GSVIVT00005194001), Stilbene synthase 5 (GSVIVT00007357001), Stilbene synthase 3 (VIT_16s0100g01030)
This subpathway is part of the pathway 3,4',5-trihydroxystilbene biosynthesis, which is itself part of Phytoalexin biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 3,4',5-trihydroxystilbene from trans-4-coumarate, the pathway 3,4',5-trihydroxystilbene biosynthesis and in Phytoalexin biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei164PROSITE-ProRule annotation1
Binding sitei267Substrate; via carbonyl oxygenBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Plant defense, Stress response

Enzyme and pathway databases

BRENDAi2.3.1.95. 6671.
UniPathwayiUPA00372; UER00548.

Names & Taxonomyi

Protein namesi
Recommended name:
Stilbene synthase 1 (EC:2.3.1.95)
Alternative name(s):
PSV25
Resveratrol synthase 1
Trihydroxystilbene synthase 1
Short name:
StSy 1
Vitis stilbene synthase 1
Gene namesi
Name:VINST1
Synonyms:STS2, VST1
ORF Names:GSVIVT00009226001, LOC100256566, VITISV_035301
OrganismiVitis vinifera (Grape)
Taxonomic identifieri29760 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsVitalesVitaceaeVitis

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Biotechnological usei

Driven by a stress-inducible promoter, stilbene synthase is used to improve resistance to pathogens which are sensitive to stilbenes such as B.cinerea, P.palmivora, E.lata or P.viticola.
Expression in heterologous systems (e.g. S.cerevisiae and Populus alba) is used to produce resveratrol for human food and medication. Resveratrol, a product of the chemical reaction catalyzed by stilbene synthase, exhibits several clinical benefits for human diseases, such as cancers, cardiovascular diseases and has positive effects on longevity.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002160821 – 392Stilbene synthase 1Add BLAST392

Proteomic databases

PRIDEiP28343.

Expressioni

Tissue specificityi

In leaves, expressed in palisade and spongy parenchyma cells and, to a lesser extent, in epidermal cells after induction.1 Publication

Inductioni

By stress, including UV light, ozone and pathogens such as B.cinerea. Strongly induced by ergosterol, a non-specific fungal elicitor. Positively regulated by jasmonate (JA) and methyl-jasmonate (MeJA). Induction by phytopathogen attack decreases with grape berry ripening.6 Publications

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi29760.VIT_16s0100g01160.t01.

Structurei

3D structure databases

ProteinModelPortaliP28343.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni55 – 58Substrate bindingBy similarity4
Regioni305 – 307Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the chalcone/stilbene synthases family.Curated

Phylogenomic databases

eggNOGiENOG410IEWP. Eukaryota.
COG3424. LUCA.
HOGENOMiHOG000245460.
KOiK13232.

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR012328. Chalcone/stilbene_synth_C.
IPR018088. Chalcone/stilbene_synthase_AS.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
[Graphical view]
PfamiPF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000451. PKS_III. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
PROSITEiPS00441. CHALCONE_SYNTH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28343-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASVEEFRNA QRAKGPATIL AIGTATPDHC VYQSDYADYY FRVTKSEHMT
60 70 80 90 100
ELKKKFNRIC DKSMIKKRYI HLTEEMLEEH PNIGAYMAPS LNIRQEIITA
110 120 130 140 150
EVPRLGRDAA LKALKEWGQP KSKITHLVFC TTSGVEMPGA DYKLANLLGL
160 170 180 190 200
ETSVRRVMLY HQGCYAGGTV LRTAKDLAEN NAGARVLVVC SEITVVTFRG
210 220 230 240 250
PSEDALDSLV GQALFGDGSS AVIVGSDPDV SIERPLFQLV SAAQTFIPNS
260 270 280 290 300
AGAIAGNLRE VGLTFHLWPN VPTLISENIE KCLTQAFDPL GISDWNSLFW
310 320 330 340 350
IAHPGGPAIL DAVEAKLNLE KKKLEATRHV LSEYGNMSSA CVLFILDEMR
360 370 380 390
KKSLKGEKAT TGEGLDWGVL FGFGPGLTIE TVVLHSVPTV TN
Length:392
Mass (Da):42,840
Last modified:October 1, 1996 - v2
Checksum:i0C2E8C2553792431
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti387 – 389VPT → IPM in S63227 (PubMed:2200709).Curated3
Sequence conflicti387 – 389VPT → IPM in ABC84860 (Ref. 4) Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S63225 mRNA. No translation available.
S63227 mRNA. No translation available.
AB046375 Genomic DNA. Translation: BAB20980.1.
DQ366302 mRNA. Translation: ABC84860.1.
AM426171 Genomic DNA. Translation: CAN60215.1.
PIRiS11044.
RefSeqiNP_001267939.1. NM_001281010.1.
XP_010662499.1. XM_010664197.1.
UniGeneiVvi.27488.
Vvi.8.

Genome annotation databases

GeneIDi100256566.
104877274.
KEGGivvi:100256566.
vvi:104877274.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S63225 mRNA. No translation available.
S63227 mRNA. No translation available.
AB046375 Genomic DNA. Translation: BAB20980.1.
DQ366302 mRNA. Translation: ABC84860.1.
AM426171 Genomic DNA. Translation: CAN60215.1.
PIRiS11044.
RefSeqiNP_001267939.1. NM_001281010.1.
XP_010662499.1. XM_010664197.1.
UniGeneiVvi.27488.
Vvi.8.

3D structure databases

ProteinModelPortaliP28343.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi29760.VIT_16s0100g01160.t01.

Proteomic databases

PRIDEiP28343.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100256566.
104877274.
KEGGivvi:100256566.
vvi:104877274.

Phylogenomic databases

eggNOGiENOG410IEWP. Eukaryota.
COG3424. LUCA.
HOGENOMiHOG000245460.
KOiK13232.

Enzyme and pathway databases

UniPathwayiUPA00372; UER00548.
BRENDAi2.3.1.95. 6671.

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR012328. Chalcone/stilbene_synth_C.
IPR018088. Chalcone/stilbene_synthase_AS.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
[Graphical view]
PfamiPF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000451. PKS_III. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
PROSITEiPS00441. CHALCONE_SYNTH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTHS1_VITVI
AccessioniPrimary (citable) accession number: P28343
Secondary accession number(s): A5AGH1, Q2HY09, Q54AD0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Genetically modified food

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.