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P28343 (THS1_VITVI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Stilbene synthase 1

EC=2.3.1.95
Alternative name(s):
PSV25
Resveratrol synthase 1
Trihydroxystilbene synthase 1
Short name=StSy 1
Vitis stilbene synthase 1
Gene names
Name:VINST1
Synonyms:STS2, VST1
ORF Names:GSVIVT00009226001, LOC100256566, VITISV_035301
OrganismVitis vinifera (Grape)
Taxonomic identifier29760 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsVitalesVitaceaeVitis

Protein attributes

Sequence length392 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mediates resistance to pathogens which are sensitive to stilbenes such as Botrytis cinerea, Eutypa lata and Plasmopora viticola by enhancing the production of phytoalexins. Confers resistance to Phytophthora palmivora when expressed in papaya. Ref.8 Ref.9 Ref.10

Catalytic activity

3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + trans-resveratrol + 4 CO2.

Pathway

Phytoalexin biosynthesis; 3,4',5-trihydroxystilbene biosynthesis; 3,4',5-trihydroxystilbene from trans-4-coumarate: step 2/2.

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm.

Tissue specificity

In leaves, expressed in palisade and spongy parenchyma cells and, to a lesser extent, in epidermal cells after induction. Ref.7

Induction

By stress, including UV light, ozone and pathogens such as B.cinerea. Strongly induced by ergosterol, a non-specific fungal elicitor. Positively regulated by jasmonate (JA) and methyl-jasmonate (MeJA). Induction by phytopathogen attack decreases with grape berry ripening. Ref.7 Ref.8 Ref.12 Ref.14 Ref.15 Ref.17

Biotechnological use

Driven by a stress-inducible promoter, stilbene synthase is used to improve resistance to pathogens which are sensitive to stilbenes such as B.cinerea, P.palmivora, E.lata or P.viticola. Ref.8 Ref.9 Ref.10 Ref.11 Ref.13 Ref.16

Expression in heterologous systems (e.g. S.cerevisiae and Populus alba) is used to produce resveratrol for human food and medication. Resveratrol, a product of the chemical reaction catalized by stilbene synthase, exhibits several clinical benefits for human diseases, such as cancers, cardiovascular diseases and has positive effects on longevity. Ref.8 Ref.9 Ref.10 Ref.11 Ref.13 Ref.16

Sequence similarities

Belongs to the chalcone/stilbene synthases family.

Ontologies

Keywords
   Biological processPlant defense
Stress response
   Cellular componentCytoplasm
   Molecular functionAcyltransferase
Transferase
   Technical termGenetically modified food
Gene Ontology (GO)
   Biological_processbiosynthetic process

Inferred from electronic annotation. Source: InterPro

defense response

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontrihydroxystilbene synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 392392Stilbene synthase 1
PRO_0000216082

Regions

Region55 – 584Substrate binding By similarity
Region305 – 3073Substrate binding By similarity

Sites

Active site1641 By similarity
Binding site2671Substrate; via carbonyl oxygen By similarity

Experimental info

Sequence conflict387 – 3893VPT → IPM in S63227. Ref.1
Sequence conflict387 – 3893VPT → IPM in ABC84860. Ref.4

Sequences

Sequence LengthMass (Da)Tools
P28343 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: 0C2E8C2553792431

FASTA39242,840
        10         20         30         40         50         60 
MASVEEFRNA QRAKGPATIL AIGTATPDHC VYQSDYADYY FRVTKSEHMT ELKKKFNRIC 

        70         80         90        100        110        120 
DKSMIKKRYI HLTEEMLEEH PNIGAYMAPS LNIRQEIITA EVPRLGRDAA LKALKEWGQP 

       130        140        150        160        170        180 
KSKITHLVFC TTSGVEMPGA DYKLANLLGL ETSVRRVMLY HQGCYAGGTV LRTAKDLAEN 

       190        200        210        220        230        240 
NAGARVLVVC SEITVVTFRG PSEDALDSLV GQALFGDGSS AVIVGSDPDV SIERPLFQLV 

       250        260        270        280        290        300 
SAAQTFIPNS AGAIAGNLRE VGLTFHLWPN VPTLISENIE KCLTQAFDPL GISDWNSLFW 

       310        320        330        340        350        360 
IAHPGGPAIL DAVEAKLNLE KKKLEATRHV LSEYGNMSSA CVLFILDEMR KKSLKGEKAT 

       370        380        390 
TGEGLDWGVL FGFGPGLTIE TVVLHSVPTV TN 

« Hide

References

« Hide 'large scale' references
[1]"Grapevine stilbene synthase cDNA only slightly differing from chalcone synthase cDNA is expressed in Escherichia coli into a catalytically active enzyme."
Melchior F., Kindl H.
FEBS Lett. 268:17-20(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Optima.
[2]"Coordinate- and elicitor-dependent expression of stilbene synthase and phenylalanine ammonia-lyase genes in Vitis cv. Optima."
Melchior F., Kindl H.
Arch. Biochem. Biophys. 288:552-557(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Optima.
[3]"Kiwifruits (Actinidia deliciosa) transformed with a Vitis stilbene synthase gene produce piceid (resveratrol-glucoside)."
Kobayashi S., Ding C., Nakamura Y., Nakajima I., Matsumoto R.
Plant Cell Rep. 19:904-910(2000)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Optima.
[4]Zhang Y., Yu O.
Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]"The grapevine genome sequence suggests ancestral hexaploidization in major angiosperm phyla."
Jaillon O., Aury J.-M., Noel B., Policriti A., Clepet C., Casagrande A., Choisne N., Aubourg S., Vitulo N., Jubin C., Vezzi A., Legeai F., Hugueney P., Dasilva C., Horner D., Mica E., Jublot D., Poulain J. expand/collapse author list , Bruyere C., Billault A., Segurens B., Gouyvenoux M., Ugarte E., Cattonaro F., Anthouard V., Vico V., Del Fabbro C., Alaux M., Di Gaspero G., Dumas V., Felice N., Paillard S., Juman I., Moroldo M., Scalabrin S., Canaguier A., Le Clainche I., Malacrida G., Durand E., Pesole G., Laucou V., Chatelet P., Merdinoglu D., Delledonne M., Pezzotti M., Lecharny A., Scarpelli C., Artiguenave F., Pe M.E., Valle G., Morgante M., Caboche M., Adam-Blondon A.-F., Weissenbach J., Quetier F., Wincker P.
Nature 449:463-467(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Pinot noir / PN40024.
[6]"A high quality draft consensus sequence of the genome of a heterozygous grapevine variety."
Velasco R., Zharkikh A., Troggio M., Cartwright D.A., Cestaro A., Pruss D., Pindo M., FitzGerald L.M., Vezzulli S., Reid J., Malacarne G., Iliev D., Coppola G., Wardell B., Micheletti D., Macalma T., Facci M., Mitchell J.T. expand/collapse author list , Perazzolli M., Eldredge G., Gatto P., Oyzerski R., Moretto M., Gutin N., Stefanini M., Chen Y., Segala C., Davenport C., Dematte L., Mraz A., Battilana J., Stormo K., Costa F., Tao Q., Si-Ammour A., Harkins T., Lackey A., Perbost C., Taillon B., Stella A., Solovyev V., Fawcett J.A., Sterck L., Vandepoele K., Grando S.M., Toppo S., Moser C., Lanchbury J., Bogden R., Skolnick M., Sgaramella V., Bhatnagar S.K., Fontana P., Gutin A., Van de Peer Y., Salamini F., Viola R.
PLoS ONE 2:E1326-E1326(2007)
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Pinot noir.
[7]"An ozone-responsive region of the grapevine resveratrol synthase promoter differs from the basal pathogen-responsive sequence."
Schubert R., Fischer R., Hain R., Schreier P.H., Bahnweg G., Ernst D., Sandermann H. Jr.
Plant Mol. Biol. 34:417-426(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION BY OZONE AND PATHOGENS, TISSUE SPECIFICITY.
[8]"In vitro tolerance to Botrytis cinerea of grapevine 41B rootstock in transgenic plants expressing the stilbene synthase Vst1 gene under the control of a pathogen-inducible PR 10 promoter."
Coutos-Thevenot P., Poinssot B., Bonomelli A., Yean H., Breda C., Buffard D., Esnault R., Hain R., Boulay M.
J. Exp. Bot. 52:901-910(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, BIOTECHNOLOGY, INDUCTION BY PHYTOPATHOGEN AND ABIOTIC STRESS.
[9]"Nucleic acid comprising the sequence of a promoter unductible by stress and a gene sequence coding for a stilbene synthase."
Coutos-Thevenot P., Hain R., Schreier P.-H., Boulay M., Esnault R.
Patent number US6800794, 05-OCT-2004
Cited for: FUNCTION, BIOTECHNOLOGY.
[10]"Expression of the grapevine stilbene synthase gene VST1 in papaya provides increased resistance against diseases caused by Phytophthora palmivora."
Zhu Y.J., Agbayani R., Jackson M.C., Tang C.S., Moore P.H.
Planta 220:241-250(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, BIOTECHNOLOGY.
[11]"Expression of the stilbene synthase (StSy) gene from grapevine in transgenic white poplar results in high accumulation of the antioxidant resveratrol glucosides."
Giorcelli A., Sparvoli F., Mattivi F., Tava A., Balestrazzi A., Vrhovsek U., Calligari P., Bollini R., Confalonieri M.
Transgenic Res. 13:203-214(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: BIOTECHNOLOGY.
[12]"Jasmonates and Na-orthovanadate promote resveratrol production in Vitis vinifera cv. Barbera cell cultures."
Tassoni A., Fornale S., Franceschetti M., Musiani F., Michael A.J., Perry B., Bagni N.
New Phytol. 166:895-905(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INDUCTION BY JA.
[13]"Antioxidant metabolite profiles in tomato fruit constitutively expressing the grapevine stilbene synthase gene."
Giovinazzo G., D'Amico L., Paradiso A., Bollini R., Sparvoli F., DeGara L.
Plant Biotechnol. J. 3:57-69(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: BIOTECHNOLOGY.
[14]"Methyl jasmonate induces defense responses in grapevine and triggers protection against Erysiphe necator."
Belhadj A., Saigne C., Telef N., Cluzet S., Bouscaut J., Corio-Costet M.-F., Merillon J.-M.
J. Agric. Food Chem. 54:9119-9125(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION BY JA.
[15]"Transgenic peas (Pisum sativum) expressing polygalacturonase inhibiting protein from raspberry (Rubus idaeus) and stilbene synthase from grape (Vitis vinifera)."
Richter A., de Kathen A., de Lorenzo G., Briviba K., Hain R., Ramsay G., Jacobsen H.-J., Kiesecker H.
Plant Cell Rep. 25:1166-1173(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION BY UV LIGHT.
[16]"Production of resveratrol in recombinant microorganisms."
Beekwilder J., Wolswinkel R., Jonker H., Hall R., de Vos C.H.R., Bovy A.
Appl. Environ. Microbiol. 72:5670-5672(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: BIOTECHNOLOGY.
[17]"Molecular basis of ergosterol-induced protection of grape against botrytis cinerea: induction of type I LTP promoter activity, WRKY, and stilbene synthase gene expression."
Laquitaine L., Gomes E., Francois J., Marchive C., Pascal S., Hamdi S., Atanassova R., Delrot S., Coutos-Thevenot P.
Mol. Plant Microbe Interact. 19:1103-1112(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION BY ERGOSTEROL.
[18]"Chemoprevention by resveratrol: molecular mechanisms and therapeutic potential."
Shankar S., Singh G., Srivastava R.K.
Front. Biosci. 12:4839-4854(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S63225 mRNA. No translation available.
S63227 mRNA. No translation available.
AB046375 Genomic DNA. Translation: BAB20980.1.
DQ366302 mRNA. Translation: ABC84860.1.
AM426171 Genomic DNA. Translation: CAN60215.1.
PIRS11044.
RefSeqNP_001267939.1. NM_001281010.1.
UniGeneVvi.27488.
Vvi.8.

3D structure databases

ProteinModelPortalP28343.
SMRP28343. Positions 3-387.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100256566.
KEGGvvi:100256566.

Phylogenomic databases

eggNOGCOG3424.
HOGENOMHOG000245460.
KOK13232.
ProtClustDBCLSN2722698.

Enzyme and pathway databases

UniPathwayUPA00372; UER00548.

Family and domain databases

Gene3D3.40.47.10. 2 hits.
InterProIPR012328. Chalcone/stilbene_synth_C.
IPR018088. Chalcone/stilbene_synthase_AS.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamPF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view]
PIRSFPIRSF000451. PKS_III. 1 hit.
SUPFAMSSF53901. SSF53901. 2 hits.
PROSITEPS00441. CHALCONE_SYNTH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTHS1_VITVI
AccessionPrimary (citable) accession number: P28343
Secondary accession number(s): A5AGH1, Q2HY09, Q54AD0
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways