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P28343

- THS1_VITVI

UniProt

P28343 - THS1_VITVI

Protein

Stilbene synthase 1

Gene

VINST1

Organism
Vitis vinifera (Grape)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Mediates resistance to pathogens which are sensitive to stilbenes such as Botrytis cinerea, Eutypa lata and Plasmopora viticola by enhancing the production of phytoalexins. Confers resistance to Phytophthora palmivora when expressed in papaya.3 Publications

    Catalytic activityi

    3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + trans-resveratrol + 4 CO2.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei164 – 1641PROSITE-ProRule annotation
    Binding sitei267 – 2671Substrate; via carbonyl oxygenBy similarity

    GO - Molecular functioni

    1. trihydroxystilbene synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. biosynthetic process Source: InterPro
    2. defense response Source: UniProtKB-KW

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Plant defense, Stress response

    Enzyme and pathway databases

    UniPathwayiUPA00372; UER00548.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Stilbene synthase 1 (EC:2.3.1.95)
    Alternative name(s):
    PSV25
    Resveratrol synthase 1
    Trihydroxystilbene synthase 1
    Short name:
    StSy 1
    Vitis stilbene synthase 1
    Gene namesi
    Name:VINST1
    Synonyms:STS2, VST1
    ORF Names:GSVIVT00009226001, LOC100256566, VITISV_035301
    OrganismiVitis vinifera (Grape)
    Taxonomic identifieri29760 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsVitalesVitaceaeVitis

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Biotechnological usei

    Driven by a stress-inducible promoter, stilbene synthase is used to improve resistance to pathogens which are sensitive to stilbenes such as B.cinerea, P.palmivora, E.lata or P.viticola.
    Expression in heterologous systems (e.g. S.cerevisiae and Populus alba) is used to produce resveratrol for human food and medication. Resveratrol, a product of the chemical reaction catalized by stilbene synthase, exhibits several clinical benefits for human diseases, such as cancers, cardiovascular diseases and has positive effects on longevity.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 392392Stilbene synthase 1PRO_0000216082Add
    BLAST

    Expressioni

    Tissue specificityi

    In leaves, expressed in palisade and spongy parenchyma cells and, to a lesser extent, in epidermal cells after induction.1 Publication

    Inductioni

    By stress, including UV light, ozone and pathogens such as B.cinerea. Strongly induced by ergosterol, a non-specific fungal elicitor. Positively regulated by jasmonate (JA) and methyl-jasmonate (MeJA). Induction by phytopathogen attack decreases with grape berry ripening.6 Publications

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP28343.
    SMRiP28343. Positions 3-387.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni55 – 584Substrate bindingBy similarity
    Regioni305 – 3073Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the chalcone/stilbene synthases family.Curated

    Phylogenomic databases

    eggNOGiCOG3424.
    HOGENOMiHOG000245460.
    KOiK00660.

    Family and domain databases

    Gene3Di3.40.47.10. 2 hits.
    InterProiIPR012328. Chalcone/stilbene_synth_C.
    IPR018088. Chalcone/stilbene_synthase_AS.
    IPR001099. Chalcone/stilbene_synthase_N.
    IPR011141. Polyketide_synthase_type-III.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view]
    PfamiPF02797. Chal_sti_synt_C. 1 hit.
    PF00195. Chal_sti_synt_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000451. PKS_III. 1 hit.
    SUPFAMiSSF53901. SSF53901. 2 hits.
    PROSITEiPS00441. CHALCONE_SYNTH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P28343-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASVEEFRNA QRAKGPATIL AIGTATPDHC VYQSDYADYY FRVTKSEHMT    50
    ELKKKFNRIC DKSMIKKRYI HLTEEMLEEH PNIGAYMAPS LNIRQEIITA 100
    EVPRLGRDAA LKALKEWGQP KSKITHLVFC TTSGVEMPGA DYKLANLLGL 150
    ETSVRRVMLY HQGCYAGGTV LRTAKDLAEN NAGARVLVVC SEITVVTFRG 200
    PSEDALDSLV GQALFGDGSS AVIVGSDPDV SIERPLFQLV SAAQTFIPNS 250
    AGAIAGNLRE VGLTFHLWPN VPTLISENIE KCLTQAFDPL GISDWNSLFW 300
    IAHPGGPAIL DAVEAKLNLE KKKLEATRHV LSEYGNMSSA CVLFILDEMR 350
    KKSLKGEKAT TGEGLDWGVL FGFGPGLTIE TVVLHSVPTV TN 392
    Length:392
    Mass (Da):42,840
    Last modified:October 1, 1996 - v2
    Checksum:i0C2E8C2553792431
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti387 – 3893VPT → IPM in S63227. (PubMed:2200709)Curated
    Sequence conflicti387 – 3893VPT → IPM in ABC84860. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S63225 mRNA. No translation available.
    S63227 mRNA. No translation available.
    AB046375 Genomic DNA. Translation: BAB20980.1.
    DQ366302 mRNA. Translation: ABC84860.1.
    AM426171 Genomic DNA. Translation: CAN60215.1.
    PIRiS11044.
    RefSeqiNP_001267939.1. NM_001281010.1.
    UniGeneiVvi.27488.
    Vvi.8.

    Genome annotation databases

    GeneIDi100256566.
    KEGGivvi:100256566.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S63225 mRNA. No translation available.
    S63227 mRNA. No translation available.
    AB046375 Genomic DNA. Translation: BAB20980.1 .
    DQ366302 mRNA. Translation: ABC84860.1 .
    AM426171 Genomic DNA. Translation: CAN60215.1 .
    PIRi S11044.
    RefSeqi NP_001267939.1. NM_001281010.1.
    UniGenei Vvi.27488.
    Vvi.8.

    3D structure databases

    ProteinModelPortali P28343.
    SMRi P28343. Positions 3-387.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100256566.
    KEGGi vvi:100256566.

    Phylogenomic databases

    eggNOGi COG3424.
    HOGENOMi HOG000245460.
    KOi K00660.

    Enzyme and pathway databases

    UniPathwayi UPA00372 ; UER00548 .

    Family and domain databases

    Gene3Di 3.40.47.10. 2 hits.
    InterProi IPR012328. Chalcone/stilbene_synth_C.
    IPR018088. Chalcone/stilbene_synthase_AS.
    IPR001099. Chalcone/stilbene_synthase_N.
    IPR011141. Polyketide_synthase_type-III.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view ]
    Pfami PF02797. Chal_sti_synt_C. 1 hit.
    PF00195. Chal_sti_synt_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000451. PKS_III. 1 hit.
    SUPFAMi SSF53901. SSF53901. 2 hits.
    PROSITEi PS00441. CHALCONE_SYNTH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Grapevine stilbene synthase cDNA only slightly differing from chalcone synthase cDNA is expressed in Escherichia coli into a catalytically active enzyme."
      Melchior F., Kindl H.
      FEBS Lett. 268:17-20(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Optima.
    2. "Coordinate- and elicitor-dependent expression of stilbene synthase and phenylalanine ammonia-lyase genes in Vitis cv. Optima."
      Melchior F., Kindl H.
      Arch. Biochem. Biophys. 288:552-557(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Optima.
    3. "Kiwifruits (Actinidia deliciosa) transformed with a Vitis stilbene synthase gene produce piceid (resveratrol-glucoside)."
      Kobayashi S., Ding C., Nakamura Y., Nakajima I., Matsumoto R.
      Plant Cell Rep. 19:904-910(2000)
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Optima.
    4. Zhang Y., Yu O.
      Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    5. "The grapevine genome sequence suggests ancestral hexaploidization in major angiosperm phyla."
      Jaillon O., Aury J.-M., Noel B., Policriti A., Clepet C., Casagrande A., Choisne N., Aubourg S., Vitulo N., Jubin C., Vezzi A., Legeai F., Hugueney P., Dasilva C., Horner D., Mica E., Jublot D., Poulain J.
      , Bruyere C., Billault A., Segurens B., Gouyvenoux M., Ugarte E., Cattonaro F., Anthouard V., Vico V., Del Fabbro C., Alaux M., Di Gaspero G., Dumas V., Felice N., Paillard S., Juman I., Moroldo M., Scalabrin S., Canaguier A., Le Clainche I., Malacrida G., Durand E., Pesole G., Laucou V., Chatelet P., Merdinoglu D., Delledonne M., Pezzotti M., Lecharny A., Scarpelli C., Artiguenave F., Pe M.E., Valle G., Morgante M., Caboche M., Adam-Blondon A.-F., Weissenbach J., Quetier F., Wincker P.
      Nature 449:463-467(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Pinot noir / PN40024.
    6. "A high quality draft consensus sequence of the genome of a heterozygous grapevine variety."
      Velasco R., Zharkikh A., Troggio M., Cartwright D.A., Cestaro A., Pruss D., Pindo M., FitzGerald L.M., Vezzulli S., Reid J., Malacarne G., Iliev D., Coppola G., Wardell B., Micheletti D., Macalma T., Facci M., Mitchell J.T.
      , Perazzolli M., Eldredge G., Gatto P., Oyzerski R., Moretto M., Gutin N., Stefanini M., Chen Y., Segala C., Davenport C., Dematte L., Mraz A., Battilana J., Stormo K., Costa F., Tao Q., Si-Ammour A., Harkins T., Lackey A., Perbost C., Taillon B., Stella A., Solovyev V., Fawcett J.A., Sterck L., Vandepoele K., Grando S.M., Toppo S., Moser C., Lanchbury J., Bogden R., Skolnick M., Sgaramella V., Bhatnagar S.K., Fontana P., Gutin A., Van de Peer Y., Salamini F., Viola R.
      PLoS ONE 2:E1326-E1326(2007)
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Pinot noir.
    7. "An ozone-responsive region of the grapevine resveratrol synthase promoter differs from the basal pathogen-responsive sequence."
      Schubert R., Fischer R., Hain R., Schreier P.H., Bahnweg G., Ernst D., Sandermann H. Jr.
      Plant Mol. Biol. 34:417-426(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY OZONE AND PATHOGENS, TISSUE SPECIFICITY.
    8. "In vitro tolerance to Botrytis cinerea of grapevine 41B rootstock in transgenic plants expressing the stilbene synthase Vst1 gene under the control of a pathogen-inducible PR 10 promoter."
      Coutos-Thevenot P., Poinssot B., Bonomelli A., Yean H., Breda C., Buffard D., Esnault R., Hain R., Boulay M.
      J. Exp. Bot. 52:901-910(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOTECHNOLOGY, INDUCTION BY PHYTOPATHOGEN AND ABIOTIC STRESS.
    9. "Nucleic acid comprising the sequence of a promoter unductible by stress and a gene sequence coding for a stilbene synthase."
      Coutos-Thevenot P., Hain R., Schreier P.-H., Boulay M., Esnault R.
      Patent number US6800794, 05-OCT-2004
      Cited for: FUNCTION, BIOTECHNOLOGY.
    10. "Expression of the grapevine stilbene synthase gene VST1 in papaya provides increased resistance against diseases caused by Phytophthora palmivora."
      Zhu Y.J., Agbayani R., Jackson M.C., Tang C.S., Moore P.H.
      Planta 220:241-250(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOTECHNOLOGY.
    11. "Expression of the stilbene synthase (StSy) gene from grapevine in transgenic white poplar results in high accumulation of the antioxidant resveratrol glucosides."
      Giorcelli A., Sparvoli F., Mattivi F., Tava A., Balestrazzi A., Vrhovsek U., Calligari P., Bollini R., Confalonieri M.
      Transgenic Res. 13:203-214(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: BIOTECHNOLOGY.
    12. "Jasmonates and Na-orthovanadate promote resveratrol production in Vitis vinifera cv. Barbera cell cultures."
      Tassoni A., Fornale S., Franceschetti M., Musiani F., Michael A.J., Perry B., Bagni N.
      New Phytol. 166:895-905(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INDUCTION BY JA.
    13. "Antioxidant metabolite profiles in tomato fruit constitutively expressing the grapevine stilbene synthase gene."
      Giovinazzo G., D'Amico L., Paradiso A., Bollini R., Sparvoli F., DeGara L.
      Plant Biotechnol. J. 3:57-69(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: BIOTECHNOLOGY.
    14. "Methyl jasmonate induces defense responses in grapevine and triggers protection against Erysiphe necator."
      Belhadj A., Saigne C., Telef N., Cluzet S., Bouscaut J., Corio-Costet M.-F., Merillon J.-M.
      J. Agric. Food Chem. 54:9119-9125(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY JA.
    15. "Transgenic peas (Pisum sativum) expressing polygalacturonase inhibiting protein from raspberry (Rubus idaeus) and stilbene synthase from grape (Vitis vinifera)."
      Richter A., de Kathen A., de Lorenzo G., Briviba K., Hain R., Ramsay G., Jacobsen H.-J., Kiesecker H.
      Plant Cell Rep. 25:1166-1173(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY UV LIGHT.
    16. Cited for: BIOTECHNOLOGY.
    17. "Molecular basis of ergosterol-induced protection of grape against botrytis cinerea: induction of type I LTP promoter activity, WRKY, and stilbene synthase gene expression."
      Laquitaine L., Gomes E., Francois J., Marchive C., Pascal S., Hamdi S., Atanassova R., Delrot S., Coutos-Thevenot P.
      Mol. Plant Microbe Interact. 19:1103-1112(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY ERGOSTEROL.
    18. "Chemoprevention by resveratrol: molecular mechanisms and therapeutic potential."
      Shankar S., Singh G., Srivastava R.K.
      Front. Biosci. 12:4839-4854(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.

    Entry informationi

    Entry nameiTHS1_VITVI
    AccessioniPrimary (citable) accession number: P28343
    Secondary accession number(s): A5AGH1, Q2HY09, Q54AD0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 104 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Genetically modified food

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3