P28339 (DPOD1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA polymerase delta catalytic subunit
EC=2.7.7.7

Gene names

Name:

POLD1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	1106 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Required with its accessory proteins (proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) or activator 1) for leading strand synthesis. Also involved in completing Okazaki fragments initiated by the DNA polymerase alpha/primase complex.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Subunit structure	Heterotetramer composed of subunits of 125 kDa, 50 kDa, 66 kDa and 12 kDa. The 125 kDa subunit contains the polymerase active site and most likely the active site for the 3'-5' exonuclease activity. Interacts with WRNIP1. Interacts with POLD4 and PCNA By similarity. Ref.2
Subcellular location	Nucleus.
Miscellaneous	In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis.
Sequence similarities	Belongs to the DNA polymerase type-B family.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	DNA-directed DNA polymerase Exonuclease Hydrolase Nuclease Nucleotidyltransferase Transferase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	DNA replication Traceable author statement Ref.2. Source: UniProtKB
Cellular component	nucleus Inferred by curator Ref.2. Source: UniProtKB
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA-directed DNA polymerase activity Traceable author statement Ref.2. Source: UniProtKB exonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1106	1106	DNA polymerase delta catalytic subunit		PRO_0000046441
Regions
	Zinc finger	1011 – 1028	18	C4-type Potential
	Zinc finger	1057 – 1075	19	C4-type Potential
	Motif	4 – 19	16	Nuclear localization signal Potential
Amino acid modifications
	Modified residue	913	1	Phosphoserine By similarity
	Modified residue	916	1	Phosphoserine By similarity
	Modified residue	1006	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P28339 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: EFC2ED155B290431	FASTA	1,106	123,709
	10 20 30 40 50 60 MDGKRRPGPG PGVPPKRARG GLWDEDEAYR PSQFEEELAL MEEMEAERRL QEQEEEELQS 70 80 90 100 110 120 ALEAADGQFS PTAIDARWLR PAPPALDPQM EPLIFQQLEI DHYVAPARPL PGAPPPSQDS 130 140 150 160 170 180 VPILRAFGVT NEGVSVCCHI HGFAPYFYTP APPGFGPEHL SELQRELSAA ISRDQRGGKE 190 200 210 220 230 240 LTGPAVLAVE LCSRESMFGY HGHGPSPFLR ITLALPRLMA PARRLLEQGI RLAGLGTPSF 250 260 270 280 290 300 APYEANVDFE IRFMVDTDIV GCNWLELPAG KYILRPEGKA TLCQLEADVL WSDVISHPPE 310 320 330 340 350 360 GEWQRIAPLR VLSFDIECAG RKGIFPEPER DPVIQICSLG LRWGEPEPFL RLALTLRPCA 370 380 390 400 410 420 PILGAKVQSY EREEDLLQAW STFIRIMDPD VITGYNIQNF DLPYLISRAQ TLKVPGFPLL 430 440 450 460 470 480 GRVIGLRSNI RESSFQSRQT GRRDSKVVSM VGRVQMDMLQ VLLREYKLRS YTLNAVSFHF 490 500 510 520 530 540 LGEQKEDVQH SIITDLQNGN DQTRRRLAVY CLKDAFLPLR LLERLMVLVN AMEMARVTGV 550 560 570 580 590 600 PLGYLLSRGQ QVKVVSQLLR QAMRQGLLMP VVKTEGGEDY TGATVIEPLK GYYDVPIATL 610 620 630 640 650 660 DFSSLYPSIM MAHNLCYTTL LRPGAAQKLG LTEDQFIKTP TGDEFVKASV RKGLLPQILE 670 680 690 700 710 720 NLLSARKRAK AELAKETDPL RRQVLDGRQL ALKVSANSVY GFTGAQVGRL PCLEISQSVT 730 740 750 760 770 780 GFGRQMIEKT KQLVETKYTV ENGYSTSAKV VYGDTDSVMC RFGVSSVAEA MALGREAADW 790 800 810 820 830 840 VSGHFPSPIR LEFEKVYFPY LLISKKRYAG LLFSSRPDAH DRMDCKGLEA VRRDNCPLVA 850 860 870 880 890 900 NLVTASLRRL LIDRDPSGAV AHAQDVISDL LCNRIDISQL VITKELTRAA ADYAGKQAHV 910 920 930 940 950 960 ELAERMRKRD PGSAPSLGDR VPYVIISAAK GVAAYMKSED PLFVLEHSLP IDTQYYLEQQ 970 980 990 1000 1010 1020 LAKPLLRIFE PILGEGRAEA VLLRGDHTRC KTVLTGKVGG LLAFAKRRNC CIGCRTVLSH 1030 1040 1050 1060 1070 1080 QGAVCKFCQP RESELYQKEV SHLSALEERF SRLWTQCQRC QGSLHEDVIC TSRDCPIFYM 1090 1100 RKKVRKDLED QERLLRRFGP PGPEAW

References

[1]	"Primary structure of the catalytic subunit of calf thymus DNA polymerase delta: sequence similarities with other DNA polymerases." Zhang J., Chung D.W., Tan C.-K., Downey K.M., Davie E.W., So A.G. Biochemistry 30:11742-11750(1991) [PubMed: 1721537] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Identification of a fourth subunit of mammalian DNA polymerase delta." Liu L., Mo J.-Y., Rodriguez-Belmonte E.M., Lee M.Y.W.T. J. Biol. Chem. 275:18739-18744(2000) [PubMed: 10751307] [Abstract] Cited for: PROTEIN SEQUENCE OF 910-930 AND 968-977, SUBUNIT. Tissue: Thymus.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M80395 mRNA. Translation: AAA30493.1.
IPI	IPI00706072.
PIR	A39299.
RefSeq	NP_776852.1. NM_174427.2.
UniGene	Bt.4749.
3D structure databases
ProteinModelPortal	P28339.
ModBase	Search...
Protein-protein interaction databases
IntAct	P28339. 4 interactions.
STRING	P28339.
Proteomic databases
PRIDE	P28339.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	281990.
KEGG	bta:281990.
Organism-specific databases
CTD	5424.
Phylogenomic databases
eggNOG	maNOG14787.
HOVERGEN	HBG051395.
PhylomeDB	P28339.
Family and domain databases
InterPro	IPR006172. DNA-dir_DNA_pol_B. IPR017964. DNA-dir_DNA_pol_B_CS. IPR006133. DNA-dir_DNA_pol_B_exonuc. IPR006134. DNA-dir_DNA_pol_B_multi_dom. IPR004578. DNA-dir_DNA_pol_B_pol2. IPR023211. DNA_pol_palm_dom. IPR012337. RNaseH-like_dom. [Graphical view]
Gene3D	G3DSA:3.90.1600.10. DNA_pol_palm_dom. 2 hits.
KO	K02327.
Pfam	PF00136. DNA_pol_B. 1 hit. PF03104. DNA_pol_B_exo1. 1 hit. [Graphical view]
PRINTS	PR00106. DNAPOLB.
SMART	SM00486. POLBc. 1 hit. [Graphical view]
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
TIGRFAMs	TIGR00592. Pol2. 1 hit.
PROSITE	PS00116. DNA_POLYMERASE_B. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DPOD1_BOVIN

Accession

Primary (citable) accession number: P28339

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	December 14, 2011
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program