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P28337 (GCST_CHICK) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Aminomethyltransferase, mitochondrial
EC=2.1.2.10
Alternative name(s):
Glycine cleavage system T protein
Short name=GCVT
Gene names
Name:AMT
OrganismGallus gallus (Chicken) [Reference proteome]
Taxonomic identifier9031 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length392 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The glycine cleavage system catalyzes the degradation of glycine.

Catalytic activity

[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3.

Subunit structure

The glycine cleavage system is composed of four proteins: P, T, L and H.

Subcellular location

Mitochondrion.

Sequence similarities

Belongs to the GcvT family.

Ontologies

Keywords
   Cellular componentMitochondrion
   DomainTransit peptide
   Molecular functionAminotransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglycine catabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentmitochondrion

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionaminomethyltransferase activity

Inferred from electronic annotation. Source: EC

transaminase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 1616Mitochondrion
Chain17 – 392376Aminomethyltransferase, mitochondrial
PRO_0000010757

Sites

Binding site2211Substrate By similarity
Binding site2501Substrate By similarity
Binding site3881Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
P28337 [UniParc].

Last modified October 1, 1993. Version 2.
Checksum: A022434038AF71E0

FASTA39242,058
        10         20         30         40         50         60 
MLRAGCRAAL ARRHLSSAPE GLKQTPLDAL HRARGGRMVP FAGWSLPVQY GRGHLESHLH 

        70         80         90        100        110        120 
TRRHCSLFDV SHMLQTRVYG RDRVRFLESL VVGDIAELRP GQGTLTLLTN ERGDIVDDLI 

       130        140        150        160        170        180 
VTNTAEDHLY VVSNAGCADK DRAVMEGRAA ELRAAGGDVH LEVSGQRAAG VQGPSMAQVL 

       190        200        210        220        230        240 
QAGLPDDLTK LTFMTSTATT VFGVPGCRVT RCGYTGEDGV EISVPAGRAV ELAERLLGCP 

       250        260        270        280        290        300 
EVWPAGLAAR DSLRLEAGLC LYGNDIDEST TPVEAGLLWT LGKRRRTAMD FPGAAIIMEQ 

       310        320        330        340        350        360 
VKEKPKRKRV GLTSVGPPLR PPAAILGPEG TPVGTVTSGC PSPSLGKNIA MGYVQAAHSR 

       370        380        390 
PGTTLTVEVR KKQHPALVTK MPFVPTHYYM AK

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References

[1]"Molecular cloning of a cDNA encoding chicken T-protein of the glycine cleavage system and expression of the functional protein in Escherichia coli. Effect of mRNA secondary structure in the translational initiation region on expression."
Okamura-Ikeda K., Fujiwara K., Motokawa Y.
J. Biol. Chem. 267:18284-18290(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Isolation and sequence determination of cDNA encoding T-protein of the glycine cleavage system."
Okamura-Ikeda K., Fujiwara K., Yamamoto M., Hiraga K., Motokawa Y.
J. Biol. Chem. 266:4917-4921(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 179-392.
Tissue: Liver.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D11162 mRNA. Translation: BAA01937.1.
IPIIPI00578487.
PIRA44167.
RefSeqNP_990119.1. NM_204788.1.
UniGeneGga.1002.

3D structure databases

ProteinModelPortalP28337.
SMRP28337. Positions 22-389.
ModBaseSearch...

Proteomic databases

PRIDEP28337.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID395566.
KEGGgga:395566.

Organism-specific databases

CTD275.

Phylogenomic databases

HOVERGENHBG005822.
KOK00605.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-12927.

Family and domain databases

Gene3D3.30.1360.120. 2 hits.
InterProIPR013977. GCV_T_C.
IPR006222. GCV_T_N.
IPR006223. GcvT.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PANTHERPTHR13847:SF5. PTHR13847:SF5. 1 hit.
PfamPF01571. GCV_T. 1 hit.
PF08669. GCV_T_C. 1 hit.
[Graphical view]
PIRSFPIRSF006487. GcvT. 1 hit.
TIGRFAMsTIGR00528. gcvT. 1 hit.
ProtoNetSearch...

Other

NextBio20815640.

Entry information

Entry nameGCST_CHICK
AccessionPrimary (citable) accession number: P28337
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: October 1, 1993
Last modified: May 1, 2013
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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