Reviewed, UniProtKB/Swiss-Prot P28337 (GCST_CHICK)

Names and origin

Protein names

Recommended name:
    Aminomethyltransferase, mitochondrial
    EC=2.1.2.10
Alternative name(s):
    Glycine cleavage system T protein
      Short name=GCVT

Gene names

Name:

AMT

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	392 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine.
Catalytic activity	[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3.
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H.
Subcellular location	Mitochondrion.
Sequence similarities	Belongs to the gcvT family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Aminotransferase Transferase
Gene Ontology (GO)
Biological process	glycine catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminomethyltransferase activity Inferred from electronic annotation. Source: EC transaminase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 16	16	Mitochondrion
	Chain	17 – 392	376	Aminomethyltransferase, mitochondrial		PRO_0000010757

Sequences

	Sequence		Length	Mass (Da)
	P28337-1 [UniParc]. Last modified October 1, 1993. Version 2. Checksum: A022434038AF71E0	FASTA	392	42,058
	10 20 30 40 50 60 MLRAGCRAAL ARRHLSSAPE GLKQTPLDAL HRARGGRMVP FAGWSLPVQY GRGHLESHLH 70 80 90 100 110 120 TRRHCSLFDV SHMLQTRVYG RDRVRFLESL VVGDIAELRP GQGTLTLLTN ERGDIVDDLI 130 140 150 160 170 180 VTNTAEDHLY VVSNAGCADK DRAVMEGRAA ELRAAGGDVH LEVSGQRAAG VQGPSMAQVL 190 200 210 220 230 240 QAGLPDDLTK LTFMTSTATT VFGVPGCRVT RCGYTGEDGV EISVPAGRAV ELAERLLGCP 250 260 270 280 290 300 EVWPAGLAAR DSLRLEAGLC LYGNDIDEST TPVEAGLLWT LGKRRRTAMD FPGAAIIMEQ 310 320 330 340 350 360 VKEKPKRKRV GLTSVGPPLR PPAAILGPEG TPVGTVTSGC PSPSLGKNIA MGYVQAAHSR 370 380 390 PGTTLTVEVR KKQHPALVTK MPFVPTHYYM AK

References

[1]	"Molecular cloning of a cDNA encoding chicken T-protein of the glycine cleavage system and expression of the functional protein in Escherichia coli. Effect of mRNA secondary structure in the translational initiation region on expression." Okamura-Ikeda K., Fujiwara K., Motokawa Y. J. Biol. Chem. 267:18284-18290(1992) [PubMed: 1526969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Isolation and sequence determination of cDNA encoding T-protein of the glycine cleavage system." Okamura-Ikeda K., Fujiwara K., Yamamoto M., Hiraga K., Motokawa Y. J. Biol. Chem. 266:4917-4921(1991) [PubMed: 2002038] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 179-392. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D11162 mRNA. Translation: BAA01937.1.
IPI	IPI00578487.
PIR	A44167.
RefSeq	NP_990119.1.
UniGene	Gga.1002
3D structure databases
SMR	P28337. Positions 22-389.
ModBase	Search...
Proteomic databases
PRIDE	P28337.
Genome annotation databases
GeneID	395566.
KEGG	gga:395566.
Phylogenomic databases
eggNOG	veNOG11023.
HOVERGEN	P28337.
PhylomeDB	P28337.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-12927.
BRENDA	2.1.2.10. 4.
Family and domain databases
InterPro	IPR013977. GCV_T_C. IPR006222. GCV_T_N. IPR006223. GcvT. [Graphical view]
Pfam	PF01571. GCV_T. 1 hit. PF08669. GCV_T_C. 1 hit. [Graphical view]
PIRSF	PIRSF006487. GcvT. 1 hit.
TIGRFAMs	TIGR00528. gcvT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GCST_CHICK

Accession

Primary (citable) accession number: P28337

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	October 1, 1993
Last modified:	February 9, 2010
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)