Reviewed,
UniProtKB/Swiss-Prot P28335 (5HT2C_HUMAN)
Last modified
November 24, 2009.
Version 105.
History...
Clusters with 100%,
90%,
50% identity |
Documents (6) |
Third-party data |
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Names and origin
| Protein names | Recommended name: 5-hydroxytryptamine receptor 2C Short name=5-HTR2C Short name=5-HT-2C Short name=5-HT2C Alternative name(s): 5HT-1C Serotonin receptor 2C | ||||
| Gene names |
| ||||
| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 458 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Ref.2 |
| Subunit structure | Interacts with MPDZ. Ref.8 |
| Subcellular location | |
| Domain | The PDZ domain-binding motif is involved in the interaction with MPDZ. Ref.8 |
| Post-translational modification | N-glycosylated. Ref.8 |
| Polymorphism | Position 23 is polymorphic; the frequencies in unrelated Caucasians are 0.87 for Cys and 0.13 for Ser. |
| Sequence similarities | Belongs to the G-protein coupled receptor 1 family. |
| RNA editing | Edited at positions 156, 158 and 160. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cell membrane Membrane |
| Coding sequence diversity | Polymorphism RNA editing |
| Domain | Transmembrane |
| Molecular function | G-protein coupled receptor Receptor Transducer |
| PTM | Disulfide bond Glycoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | G-protein coupled receptor protein signaling pathway Inferred from Experiment. Source: Reactome feeding behaviorTraceable author statement. Source: ProtInc synaptic transmissionTraceable author statement. Source: ProtInc |
| Cellular component | cytoplasm Inferred from direct assay. Source: HPA integral to membraneInferred from electronic annotation. Source: UniProtKB-SubCell nucleusInferred from direct assay. Source: HPA plasma membraneInferred from direct assay. Source: HPA |
| Molecular function | protein binding Inferred from physical interaction. Source: IntAct serotonin bindingInferred from direct assay. Source: MGI serotonin receptor activityInferred from direct assay. Source: MGI |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| Arrb2 | Q91YI4 | 1 | EBI-994141,EBI-994161 | From a different organism. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 458 | 458 | 5-hydroxytryptamine receptor 2C | PRO_0000068958 | |||||||
Regions | |||||||||||
| Topological domain | 1 – 52 | 52 | Extracellular By similarity | ||||||||
| Transmembrane | 53 – 78 | 26 | 1 By similarity | ||||||||
| Topological domain | 79 – 89 | 11 | Cytoplasmic By similarity | ||||||||
| Transmembrane | 90 – 110 | 21 | 2 By similarity | ||||||||
| Topological domain | 111 – 127 | 17 | Extracellular By similarity | ||||||||
| Transmembrane | 128 – 150 | 23 | 3 By similarity | ||||||||
| Topological domain | 151 – 170 | 20 | Cytoplasmic By similarity | ||||||||
| Transmembrane | 171 – 193 | 23 | 4 By similarity | ||||||||
| Topological domain | 194 – 213 | 20 | Extracellular By similarity | ||||||||
| Transmembrane | 214 – 235 | 22 | 5 By similarity | ||||||||
| Topological domain | 236 – 311 | 76 | Cytoplasmic By similarity | ||||||||
| Transmembrane | 312 – 333 | 22 | 6 By similarity | ||||||||
| Topological domain | 334 – 348 | 15 | Extracellular By similarity | ||||||||
| Transmembrane | 349 – 371 | 23 | 7 By similarity | ||||||||
| Topological domain | 372 – 458 | 87 | Cytoplasmic By similarity | ||||||||
| Motif | 456 – 458 | 3 | PDZ-binding | ||||||||
Amino acid modifications | |||||||||||
| Glycosylation | 39 | 1 | N-linked (GlcNAc...) Probable | ||||||||
| Disulfide bond | 127 ↔ 207 | By similarity | |||||||||
Natural variations | |||||||||||
| Natural variant | 23 | 1 | C → S: dbSNP rs6318. Ref.9 Ref.10 Ref.11 Ref.12 | VAR_003450 | |||||||
| Natural variant | 156 | 1 | I → V in RNA edited version. | VAR_010166 | |||||||
| Natural variant | 158 | 1 | N → S in RNA edited version. | VAR_010167 | |||||||
| Natural variant | 160 | 1 | I → V in RNA edited version. | VAR_010168 | |||||||
Experimental info | |||||||||||
| Mutagenesis | 456 | 1 | S → A: Loss of interaction with MPDZ. Ref.8 | ||||||||
| Mutagenesis | 456 | 1 | S → T: No effect on interaction with MPDZ. Ref.8 | ||||||||
| Mutagenesis | 457 | 1 | S → A: No effect on interaction with MPDZ. Ref.8 | ||||||||
| Mutagenesis | 458 | 1 | V → A: Loss of interaction with MPDZ. Ref.8 | ||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Cloning of the human serotonin 5-HT2 and 5-HT1C receptor subtypes." Saltzman A.G., Morse B., Whitman M.M., Ivanshchenko Y., Jaye M., Felder S. Biochem. Biophys. Res. Commun. 181:1469-1478(1991) [PubMed: 1722404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain. |
| [2] | "Genomic organisation and functional expression of the gene encoding the human serotonin 5-HT2C receptor." Stam N.J., Vanderheyden P., Van Alebeek C., Klomp J., De Boer T., Van Delft A.M.L., Olijve W. Eur. J. Pharmacol. 269:339-348(1994) [PubMed: 7895773] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Tissue: Hippocampus and Placenta. |
| [3] | "The human serotonin 5-HT2C receptor: complete cDNA, genomic structure, and alternatively spliced variant." Xie E., Zhao L., Levine A.J., Shenk T., Chang L.-S. Genomics 35:551-561(1996) [PubMed: 8812491] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain. |
| [4] | "Identification and characterization of RNA editing events within the 5-HT2C receptor." Niswender C.M., Sanders-Bush E., Emeson R.B. Ann. N. Y. Acad. Sci. 861:38-48(1998) [PubMed: 9928237] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], RNA EDITING OF POSITIONS 156; 158 AND 160. Tissue: Brain. |
| [5] | "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain. |
| [6] | "The DNA sequence of the human X chromosome." Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C. Bentley D.R.Nature 434:325-337(2005) [PubMed: 15772651] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [7] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [8] | "Interaction of serotonin 5-hydroxytryptamine type 2C receptors with PDZ10 of the multi-PDZ domain protein MUPP1." Becamel C., Figge A., Poliak S., Dumuis A., Peles E., Bockaert J., Luebbert H., Ullmer C. J. Biol. Chem. 276:12974-12982(2001) [PubMed: 11150294] [Abstract] Cited for: INTERACTION WITH MPDZ, DOMAIN, MUTAGENESIS OF SER-456; SER-457 AND VAL-458, GLYCOSYLATION. |
| [9] | "Identification, expression, and pharmacology of a Cys23-Ser23 substitution in the human 5-HT2c receptor gene (HTR2C)." Lappalainen J., Zhang L., Dean M., Oz M., Ozaki N., Yu D., Virkkunen M., Weight F., Linnoila M., Goldman D. Genomics 27:274-279(1995) [PubMed: 7557992] [Abstract] Cited for: VARIANT SER-23. |
| [10] | "Association analysis between a Cys23Ser substitution polymorphism of the human 5-HT2c receptor gene and neuronal hyperexcitability." Samochowiec J., Smolka M., Winterer G., Rommelspacher H., Schmidt L.G., Sander T. Am. J. Med. Genet. 88:126-130(1999) [PubMed: 10206230] [Abstract] Cited for: VARIANT SER-23. |
| [11] | "Unified approach to the analysis of genetic variation in serotonergic pathways." Marshall S.E., Bird T.G., Hart K., Welsh K.I. Am. J. Med. Genet. 88:621-627(1999) [PubMed: 10581480] [Abstract] Cited for: VARIANT SER-23. |
| [12] | "Characterization of single-nucleotide polymorphisms in coding regions of human genes." Cargill M., Altshuler D., Ireland J., Sklar P., Ardlie K., Patil N., Shaw N., Lane C.R., Lim E.P., Kalyanaraman N., Nemesh J., Ziaugra L., Friedland L., Rolfe A., Warrington J., Lipshutz R., Daley G.Q., Lander E.S. Nat. Genet. 22:231-238(1999) [PubMed: 10391209] [Abstract] Cited for: VARIANT SER-23. |
| [13] | Erratum Cargill M., Altshuler D., Ireland J., Sklar P., Ardlie K., Patil N., Shaw N., Lane C.R., Lim E.P., Kalyanaraman N., Nemesh J., Ziaugra L., Friedland L., Rolfe A., Warrington J., Lipshutz R., Daley G.Q., Lander E.S. Nat. Genet. 23:373-373(1999) |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| M81778 mRNA. Translation: AAA60317.1. X80763 Genomic DNA. Translation: CAB59978.1. U49516 mRNA. Translation: AAB40898.1. AF208053 mRNA. Translation: AAF35842.1. AF498983 mRNA. Translation: AAM21130.1. AC004822 Genomic DNA. Translation: AAC71658.1. AL355812, AL590097, AC004822 Genomic DNA. Translation: CAI41335.1. AL590097, AC004822, AL355812 Genomic DNA. Translation: CAH70193.1. BC095543 mRNA. Translation: AAH95543.1. | |
| IPI | IPI00002876. |
| PIR | JS0616. |
| RefSeq | NP_000859.1. |
| UniGene | Hs.149037 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P28335. 1 interaction. |
| STRING | P28335. |
Protein family/group databases | |
| GPCRDB | Search... |
PTM databases | |
| PhosphoSite | P28335. |
Proteomic databases | |
| PRIDE | P28335. |
Genome annotation databases | |
| Ensembl | ENST00000276198; ENSP00000276198; ENSG00000147246; Homo sapiens. [Genome view] ENST00000371951; ENSP00000361019; ENSG00000147246; Homo sapiens. [Genome view] |
| GeneID | 3358. |
| KEGG | hsa:3358. |
| UCSC | uc004epu.1. human. |
Organism-specific databases | |
| CTD | 3358. |
| GeneCards | GC0XP113641. |
| H-InvDB | HIX0028341. |
| HGNC | HGNC:5295. HTR2C. |
| HPA | CAB006857. HPA003133. |
| MIM | 312861. gene. |
| PharmGKB | PA194. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOGENOM | P28335. |
| HOVERGEN | P28335. |
| OMA | NPSNVVS |
| OrthoDB | EOG9ZSCNH |
Enzyme and pathway databases | |
| Reactome | REACT_14797. Signaling by GPCR. |
Gene expression databases | |
| ArrayExpress | P28335. |
| Bgee | P28335. |
| CleanEx | HS_HTR2C. |
| Genevestigator | P28335. |
| GermOnline | ENSG00000147246. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR000377. 5HT2C_rcpt. IPR002231. 5HT_rcpt. IPR000276. 7TM_GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. [Graphical view] |
| Pfam | PF00001. 7tm_1. 1 hit. [Graphical view] |
| PRINTS | PR00517. 5HT2CRECEPTR. PR01101. 5HTRECEPTOR. PR00237. GPCRRHODOPSN. |
| PROSITE | PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| DrugBank | DB01239. Chlorprothixene. DB00363. Clozapine. DB01191. Dexfenfluramine. DB00574. Fenfluramine. DB00247. Methysergide. DB06148. Mianserin. DB00805. Minaprine. DB00370. Mirtazapine. DB00334. Olanzapine. DB00420. Promazine. DB00777. Propiomazine. DB01224. Quetiapine. DB06144. Sertindole. DB00372. Thiethylperazine. DB00193. Tramadol. DB00246. Ziprasidone. |
| NextBio | 13278. |
| SOURCE | Search... |
Entry information
| Entry name | 5HT2C_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P28335 Secondary accession number(s): Q5VUF8, Q9NP28 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| 7-transmembrane G-linked receptors List of 7-transmembrane G-linked receptor entries |
| Human chromosome X Human chromosome X: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


