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P28334

- 5HT1B_MOUSE

UniProt

P28334 - 5HT1B_MOUSE

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Protein

5-hydroxytryptamine receptor 1B

Gene

Htr1b

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various alkaloids and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity. Arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Regulates the release of 5-hydroxytryptamine, dopamine and acetylcholine in the brain, and thereby affects neural activity, nociceptive processing, pain perception, mood and behavior. Besides, plays a role in vasoconstriction of cerebral arteries.6 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei351 – 3511Important for species-specific agonist sensitivityBy similarity

GO - Molecular functioni

  1. drug binding Source: UniProtKB
  2. serotonin binding Source: UniProtKB
  3. serotonin receptor activity Source: UniProtKB

GO - Biological processi

  1. adenylate cyclase-inhibiting serotonin receptor signaling pathway Source: UniProtKB
  2. bone remodeling Source: MGI
  3. cellular response to alkaloid Source: UniProtKB
  4. cellular response to drug Source: UniProtKB
  5. cellular response to temperature stimulus Source: UniProtKB
  6. drinking behavior Source: Ensembl
  7. G-protein coupled receptor internalization Source: UniProtKB
  8. negative regulation of cAMP biosynthetic process Source: Ensembl
  9. negative regulation of serotonin secretion Source: UniProtKB
  10. negative regulation of synaptic transmission, GABAergic Source: Ensembl
  11. negative regulation of synaptic transmission, glutamatergic Source: Ensembl
  12. protein kinase C-activating G-protein coupled receptor signaling pathway Source: UniProtKB
  13. regulation of behavior Source: InterPro
  14. regulation of dopamine secretion Source: Ensembl
  15. response to cocaine Source: Ensembl
  16. response to drug Source: UniProtKB
  17. response to ethanol Source: Ensembl
  18. response to mineralocorticoid Source: Ensembl
  19. synaptic transmission Source: Ensembl
  20. vasoconstriction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Behavior

Names & Taxonomyi

Protein namesi
Recommended name:
5-hydroxytryptamine receptor 1B
Short name:
5-HT-1B
Short name:
5-HT1B
Alternative name(s):
Serotonin receptor 1B
Gene namesi
Name:Htr1b
Synonyms:5ht1b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:96274. Htr1b.

Subcellular locationi

Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. integral component of plasma membrane Source: UniProtKB
  3. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutant male mice display increased aggressivity towards intruders. Treatment with a Htr1b agonist does not trigger increased locomotor behavior in mutant mice, contrary to what is observed with wild-type mice. Treatment with a Htr1b agonist does not inhibit 5-hydroxytryptamine release in the frontal cortex and hippocampus of mutant mice, contrary to what is observed with wild-type mice. Likewise, Htr1b agonists do not inhibit dopamine and acetylcholine release in brains from mutant mice.5 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3863865-hydroxytryptamine receptor 1BPRO_0000068917Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi24 – 241N-linked (GlcNAc...)Sequence Analysis
Glycosylationi28 – 281N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi118 ↔ 195PROSITE-ProRule annotation
Lipidationi384 – 3841S-palmitoyl cysteineSequence Analysis

Post-translational modificationi

Phosphorylated.By similarity
Palmitoylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PRIDEiP28334.

PTM databases

PhosphoSiteiP28334.

Expressioni

Tissue specificityi

Predominantly expressed in striatum and Purkinje cells.1 Publication

Gene expression databases

BgeeiP28334.
CleanExiMM_HTR1B.
ExpressionAtlasiP28334. baseline and differential.
GenevestigatoriP28334.

Interactioni

Subunit structurei

Homodimer. Heterodimer with HTR1D (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000050898.

Structurei

3D structure databases

ProteinModelPortaliP28334.
SMRiP28334. Positions 34-383.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4545ExtracellularBy similarityAdd
BLAST
Topological domaini72 – 809CytoplasmicBy similarity
Topological domaini107 – 11913ExtracellularBy similarityAdd
BLAST
Topological domaini142 – 16120CytoplasmicBy similarityAdd
BLAST
Topological domaini184 – 20118ExtracellularBy similarityAdd
BLAST
Topological domaini225 – 31187CytoplasmicBy similarityAdd
BLAST
Topological domaini333 – 34513ExtracellularBy similarityAdd
BLAST
Topological domaini368 – 38619CytoplasmicBy similarityAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei46 – 7126Helical; Name=1By similarityAdd
BLAST
Transmembranei81 – 10626Helical; Name=2By similarityAdd
BLAST
Transmembranei120 – 14122Helical; Name=3By similarityAdd
BLAST
Transmembranei162 – 18322Helical; Name=4By similarityAdd
BLAST
Transmembranei202 – 22423Helical; Name=5By similarityAdd
BLAST
Transmembranei312 – 33221Helical; Name=6By similarityAdd
BLAST
Transmembranei346 – 36722Helical; Name=7By similarityAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni121 – 13010Agonist bindingBy similarity
Regioni323 – 3275Agonist bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi142 – 1443DRY motif; important for ligand-induced conformation changes and signalingBy similarity
Motifi361 – 3655NPxxY motif; important for ligand-induced conformation changes and signalingBy similarity

Domaini

Ligands are bound in a hydrophobic pocket formed by the transmembrane helices.By similarity

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG249628.
HOGENOMiHOG000239242.
HOVERGENiHBG106962.
InParanoidiP28334.
KOiK04153.
OMAiIALPWKV.
OrthoDBiEOG7NCV3Q.
PhylomeDBiP28334.
TreeFamiTF316350.

Family and domain databases

Gene3Di1.20.1070.10. 2 hits.
InterProiIPR002147. 5HT1B_rcpt.
IPR002231. 5HT_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24247:SF16. PTHR24247:SF16. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00513. 5HT1BRECEPTR.
PR01101. 5HTRECEPTOR.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28334-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEEQGIQCAP PPPAASQTGV PLTNLSHNCS ADGYIYQDSI ALPWKVLLVA
60 70 80 90 100
LLALITLATT LSNAFVIATV YRTRKLHTPA NYLIASLAVT DLLVSILVMP
110 120 130 140 150
ISTMYTVTGR WTLGQVVCDF WLSSDITCCT ASIMHLCVIA LDRYWAITDA
160 170 180 190 200
VEYSAKRTPK RAAIMIVLVW VFSISISLPP FFWRQAKAEE EMLDCFVNTD
210 220 230 240 250
HVLYTVYSTV GAFYLPTLLL IALYGRIYVE ARSRILKQTP NKTGKRLTRA
260 270 280 290 300
QLITDSPGST SSVTSINSRA PDVPSESGSP VYVNQVKVRV SDALLEKKKL
310 320 330 340 350
MAARERKATK TLGIILGAFI VCWLPFFIIS LVMPICKDAC WFHMAIFDFF
360 370 380
NWLGYLNSLI NPIIYTMSNE DFKQAFHKLI RFKCAG
Length:386
Mass (Da):43,079
Last modified:December 1, 1992 - v1
Checksum:i58F70FBEA770C0B3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z11597 Genomic DNA. Translation: CAA77678.1.
M85151 Genomic DNA. Translation: AAA83221.1.
CCDSiCCDS23370.1.
PIRiA42688.
RefSeqiNP_034612.1. NM_010482.1.
UniGeneiMm.445308.

Genome annotation databases

EnsembliENSMUST00000051005; ENSMUSP00000050898; ENSMUSG00000049511.
ENSMUST00000183482; ENSMUSP00000139389; ENSMUSG00000049511.
GeneIDi15551.
KEGGimmu:15551.
UCSCiuc009qvl.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z11597 Genomic DNA. Translation: CAA77678.1 .
M85151 Genomic DNA. Translation: AAA83221.1 .
CCDSi CCDS23370.1.
PIRi A42688.
RefSeqi NP_034612.1. NM_010482.1.
UniGenei Mm.445308.

3D structure databases

ProteinModelPortali P28334.
SMRi P28334. Positions 34-383.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000050898.

Chemistry

BindingDBi P28334.
GuidetoPHARMACOLOGYi 2.

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei P28334.

Proteomic databases

PRIDEi P28334.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000051005 ; ENSMUSP00000050898 ; ENSMUSG00000049511 .
ENSMUST00000183482 ; ENSMUSP00000139389 ; ENSMUSG00000049511 .
GeneIDi 15551.
KEGGi mmu:15551.
UCSCi uc009qvl.1. mouse.

Organism-specific databases

CTDi 3351.
MGIi MGI:96274. Htr1b.

Phylogenomic databases

eggNOGi NOG249628.
HOGENOMi HOG000239242.
HOVERGENi HBG106962.
InParanoidi P28334.
KOi K04153.
OMAi IALPWKV.
OrthoDBi EOG7NCV3Q.
PhylomeDBi P28334.
TreeFami TF316350.

Miscellaneous databases

NextBioi 288484.
PROi P28334.
SOURCEi Search...

Gene expression databases

Bgeei P28334.
CleanExi MM_HTR1B.
ExpressionAtlasi P28334. baseline and differential.
Genevestigatori P28334.

Family and domain databases

Gene3Di 1.20.1070.10. 2 hits.
InterProi IPR002147. 5HT1B_rcpt.
IPR002231. 5HT_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view ]
PANTHERi PTHR24247:SF16. PTHR24247:SF16. 1 hit.
Pfami PF00001. 7tm_1. 1 hit.
[Graphical view ]
PRINTSi PR00513. 5HT1BRECEPTR.
PR01101. 5HTRECEPTOR.
PR00237. GPCRRHODOPSN.
PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Mouse 5HT1B serotonin receptor: cloning, functional expression, and localization in motor control centers."
    Maroteaux L., Saudou F., Amlaiky N., Boschert U., Plassat J.-L., Hen R.
    Proc. Natl. Acad. Sci. U.S.A. 89:3020-3024(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  2. "Enhanced aggressive behavior in mice lacking 5-HT1B receptor."
    Saudou F., Amara D.A., Dierich A., LeMeur M., Ramboz S., Segu L., Buhot M.C., Hen R.
    Science 265:1875-1878(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION.
  3. "Regulation of [3H]5-HT release in raphe, frontal cortex and hippocampus of 5-HT1B knock-out mice."
    Pineyro G., Castanon N., Hen R., Blier P.
    NeuroReport 7:353-359(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION.
  4. "Regulation of serotonin release in the frontal cortex and ventral hippocampus of homozygous mice lacking 5-HT1B receptors: in vivo microdialysis studies."
    Trillat A.C., Malagie I., Scearce K., Pons D., Anmella M.C., Jacquot C., Hen R., Gardier A.M.
    J. Neurochem. 69:2019-2025(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION.
  5. "5-HT1B receptors modulate release of [3H]dopamine from rat striatal synaptosomes: further evidence using 5-HT moduline, polyclonal 5-HT1B receptor antibodies and 5-HT1B receptor knock-out mice."
    Sarhan H., Grimaldi B., Hen R., Fillion G.
    Naunyn Schmiedebergs Arch. Pharmacol. 361:12-18(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION.
  6. "Presynaptic serotonergic modulation of 5-HT and acetylcholine release in the hippocampus and the cortex of 5-HT1B-receptor knockout mice."
    Rutz S., Riegert C., Rothmaier A.K., Buhot M.C., Cassel J.C., Jackisch R.
    Brain Res. Bull. 70:81-93(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION.

Entry informationi

Entry namei5HT1B_MOUSE
AccessioniPrimary (citable) accession number: P28334
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: October 29, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

A residue in the 7th transmembrane region ('Thr-355' in human, Asn-351 in mouse and rat) is important for species-specific sensitivity to various agonists.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3