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Protein

5-hydroxytryptamine receptor 1B

Gene

Htr1b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various alkaloids and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity. Arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Regulates the release of 5-hydroxytryptamine, dopamine and acetylcholine in the brain, and thereby affects neural activity, nociceptive processing, pain perception, mood and behavior. Besides, plays a role in vasoconstriction of cerebral arteries.6 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei351Important for species-specific agonist sensitivityBy similarity1

GO - Molecular functioni

  • drug binding Source: UniProtKB
  • G-protein coupled serotonin receptor activity Source: UniProtKB
  • neurotransmitter receptor activity Source: GO_Central
  • serotonin binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Behavior

Enzyme and pathway databases

ReactomeiR-MMU-390666. Serotonin receptors.
R-MMU-418594. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
5-hydroxytryptamine receptor 1B
Short name:
5-HT-1B
Short name:
5-HT1B
Alternative name(s):
Serotonin receptor 1B
Gene namesi
Name:Htr1b
Synonyms:5ht1b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:96274. Htr1b.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 45ExtracellularBy similarityAdd BLAST45
Transmembranei46 – 71Helical; Name=1By similarityAdd BLAST26
Topological domaini72 – 80CytoplasmicBy similarity9
Transmembranei81 – 106Helical; Name=2By similarityAdd BLAST26
Topological domaini107 – 119ExtracellularBy similarityAdd BLAST13
Transmembranei120 – 141Helical; Name=3By similarityAdd BLAST22
Topological domaini142 – 161CytoplasmicBy similarityAdd BLAST20
Transmembranei162 – 183Helical; Name=4By similarityAdd BLAST22
Topological domaini184 – 201ExtracellularBy similarityAdd BLAST18
Transmembranei202 – 224Helical; Name=5By similarityAdd BLAST23
Topological domaini225 – 311CytoplasmicBy similarityAdd BLAST87
Transmembranei312 – 332Helical; Name=6By similarityAdd BLAST21
Topological domaini333 – 345ExtracellularBy similarityAdd BLAST13
Transmembranei346 – 367Helical; Name=7By similarityAdd BLAST22
Topological domaini368 – 386CytoplasmicBy similarityAdd BLAST19

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • dendrite Source: GO_Central
  • integral component of plasma membrane Source: UniProtKB
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutant male mice display increased aggressivity towards intruders. Treatment with a Htr1b agonist does not trigger increased locomotor behavior in mutant mice, contrary to what is observed with wild-type mice. Treatment with a Htr1b agonist does not inhibit 5-hydroxytryptamine release in the frontal cortex and hippocampus of mutant mice, contrary to what is observed with wild-type mice. Likewise, Htr1b agonists do not inhibit dopamine and acetylcholine release in brains from mutant mice.5 Publications

Chemistry databases

GuidetoPHARMACOLOGYi2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000689171 – 3865-hydroxytryptamine receptor 1BAdd BLAST386

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi24N-linked (GlcNAc...)Sequence analysis1
Glycosylationi28N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi118 ↔ 195PROSITE-ProRule annotation
Lipidationi384S-palmitoyl cysteineSequence analysis1

Post-translational modificationi

Phosphorylated.By similarity
Palmitoylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP28334.
PRIDEiP28334.

PTM databases

iPTMnetiP28334.
PhosphoSitePlusiP28334.

Expressioni

Tissue specificityi

Predominantly expressed in striatum and Purkinje cells.1 Publication

Gene expression databases

BgeeiENSMUSG00000049511.
CleanExiMM_HTR1B.
ExpressionAtlasiP28334. baseline and differential.
GenevisibleiP28334. MM.

Interactioni

Subunit structurei

Homodimer. Heterodimer with HTR1D (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000050898.

Structurei

3D structure databases

ProteinModelPortaliP28334.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni121 – 130Agonist bindingBy similarity10
Regioni323 – 327Agonist bindingBy similarity5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi142 – 144DRY motif; important for ligand-induced conformation changes and signalingBy similarity3
Motifi361 – 365NPxxY motif; important for ligand-induced conformation changes and signalingBy similarity5

Domaini

Ligands are bound in a hydrophobic pocket formed by the transmembrane helices.By similarity

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118795.
HOGENOMiHOG000239242.
HOVERGENiHBG106962.
InParanoidiP28334.
KOiK04153.
OMAiVNTDHVL.
OrthoDBiEOG091G06VI.
PhylomeDBiP28334.
TreeFamiTF316350.

Family and domain databases

InterProiIPR002147. 5HT1B_rcpt.
IPR002231. 5HT_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24247:SF16. PTHR24247:SF16. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00513. 5HT1BRECEPTR.
PR01101. 5HTRECEPTOR.
PR00237. GPCRRHODOPSN.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28334-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEQGIQCAP PPPAASQTGV PLTNLSHNCS ADGYIYQDSI ALPWKVLLVA
60 70 80 90 100
LLALITLATT LSNAFVIATV YRTRKLHTPA NYLIASLAVT DLLVSILVMP
110 120 130 140 150
ISTMYTVTGR WTLGQVVCDF WLSSDITCCT ASIMHLCVIA LDRYWAITDA
160 170 180 190 200
VEYSAKRTPK RAAIMIVLVW VFSISISLPP FFWRQAKAEE EMLDCFVNTD
210 220 230 240 250
HVLYTVYSTV GAFYLPTLLL IALYGRIYVE ARSRILKQTP NKTGKRLTRA
260 270 280 290 300
QLITDSPGST SSVTSINSRA PDVPSESGSP VYVNQVKVRV SDALLEKKKL
310 320 330 340 350
MAARERKATK TLGIILGAFI VCWLPFFIIS LVMPICKDAC WFHMAIFDFF
360 370 380
NWLGYLNSLI NPIIYTMSNE DFKQAFHKLI RFKCAG
Length:386
Mass (Da):43,079
Last modified:December 1, 1992 - v1
Checksum:i58F70FBEA770C0B3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11597 Genomic DNA. Translation: CAA77678.1.
M85151 Genomic DNA. Translation: AAA83221.1.
CCDSiCCDS23370.1.
PIRiA42688.
RefSeqiNP_034612.1. NM_010482.1.
UniGeneiMm.445308.

Genome annotation databases

EnsembliENSMUST00000051005; ENSMUSP00000050898; ENSMUSG00000049511.
ENSMUST00000183482; ENSMUSP00000139389; ENSMUSG00000049511.
GeneIDi15551.
KEGGimmu:15551.
UCSCiuc009qvl.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11597 Genomic DNA. Translation: CAA77678.1.
M85151 Genomic DNA. Translation: AAA83221.1.
CCDSiCCDS23370.1.
PIRiA42688.
RefSeqiNP_034612.1. NM_010482.1.
UniGeneiMm.445308.

3D structure databases

ProteinModelPortaliP28334.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000050898.

Chemistry databases

GuidetoPHARMACOLOGYi2.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiP28334.
PhosphoSitePlusiP28334.

Proteomic databases

PaxDbiP28334.
PRIDEiP28334.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000051005; ENSMUSP00000050898; ENSMUSG00000049511.
ENSMUST00000183482; ENSMUSP00000139389; ENSMUSG00000049511.
GeneIDi15551.
KEGGimmu:15551.
UCSCiuc009qvl.1. mouse.

Organism-specific databases

CTDi3351.
MGIiMGI:96274. Htr1b.

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118795.
HOGENOMiHOG000239242.
HOVERGENiHBG106962.
InParanoidiP28334.
KOiK04153.
OMAiVNTDHVL.
OrthoDBiEOG091G06VI.
PhylomeDBiP28334.
TreeFamiTF316350.

Enzyme and pathway databases

ReactomeiR-MMU-390666. Serotonin receptors.
R-MMU-418594. G alpha (i) signalling events.

Miscellaneous databases

PROiP28334.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000049511.
CleanExiMM_HTR1B.
ExpressionAtlasiP28334. baseline and differential.
GenevisibleiP28334. MM.

Family and domain databases

InterProiIPR002147. 5HT1B_rcpt.
IPR002231. 5HT_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24247:SF16. PTHR24247:SF16. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00513. 5HT1BRECEPTR.
PR01101. 5HTRECEPTOR.
PR00237. GPCRRHODOPSN.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry namei5HT1B_MOUSE
AccessioniPrimary (citable) accession number: P28334
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: November 2, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

A residue in the 7th transmembrane region ('Thr-355' in human, Asn-351 in mouse and rat) is important for species-specific sensitivity to various agonists.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.