P28334 (5HT1B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-hydroxytryptamine receptor 1B
Short name=5-HT-1B
Short name=5-HT1B

Alternative name(s):
Serotonin receptor 1B

Gene names

Name:	Htr1b
Synonyms:	5ht1b

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	386 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Predominantly expressed in striatum and Purkinje cells.
Post-translational modification	Phosphorylated By similarity. Palmitoylated By similarity.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G-protein coupled receptor internalization Inferred from direct assay PubMed 17325130. Source: UniProtKB bone remodeling Inferred from genetic interaction PubMed 19041748. Source: MGI cellular response to temperature stimulus Inferred from direct assay PubMed 17325130. Source: UniProtKB drinking behavior Inferred from electronic annotation. Source: Compara negative regulation of cAMP biosynthetic process Inferred from electronic annotation. Source: Compara negative regulation of synaptic transmission, GABAergic Inferred from electronic annotation. Source: Compara negative regulation of synaptic transmission, glutamatergic Inferred from electronic annotation. Source: Compara protein kinase C-activating G-protein coupled receptor signaling pathway Inferred from mutant phenotype PubMed 17325130. Source: UniProtKB regulation of dopamine secretion Inferred from electronic annotation. Source: Compara response to cocaine Inferred from electronic annotation. Source: Compara response to drug Inferred from direct assay PubMed 17325130. Source: UniProtKB response to ethanol Inferred from electronic annotation. Source: Compara response to mineralocorticoid stimulus Inferred from electronic annotation. Source: Compara synaptic transmission Inferred from electronic annotation. Source: Compara
Cellular_component	cytoplasm Inferred from direct assay PubMed 17325130. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay PubMed 17325130. Source: UniProtKB
Molecular_function	drug binding Inferred from direct assay PubMed 17325130. Source: UniProtKB serotonin binding Inferred from direct assay PubMed 17325130. Source: UniProtKB serotonin receptor activity Inferred from direct assay PubMed 17325130. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 386	386	5-hydroxytryptamine receptor 1B		PRO_0000068917
Regions
	Topological domain	1 – 47	47	Extracellular By similarity
	Transmembrane	48 – 71	24	Helical; Name=1; By similarity
	Topological domain	72 – 82	11	Cytoplasmic By similarity
	Transmembrane	83 – 109	27	Helical; Name=2; By similarity
	Topological domain	110 – 119	10	Extracellular By similarity
	Transmembrane	120 – 141	22	Helical; Name=3; By similarity
	Topological domain	142 – 163	22	Cytoplasmic By similarity
	Transmembrane	164 – 183	20	Helical; Name=4; By similarity
	Topological domain	184 – 204	21	Extracellular By similarity
	Transmembrane	205 – 224	20	Helical; Name=5; By similarity
	Topological domain	225 – 311	87	Cytoplasmic By similarity
	Transmembrane	312 – 332	21	Helical; Name=6; By similarity
	Topological domain	333 – 346	14	Extracellular By similarity
	Transmembrane	347 – 369	23	Helical; Name=7; By similarity
	Topological domain	370 – 386	17	Cytoplasmic By similarity
Amino acid modifications
	Lipidation	384	1	S-palmitoyl cysteine Potential
	Glycosylation	24	1	N-linked (GlcNAc...) Potential
	Glycosylation	28	1	N-linked (GlcNAc...) Potential
	Disulfide bond	118 ↔ 195		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P28334 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 58F70FBEA770C0B3	FASTA	386	43,079
	10 20 30 40 50 60 MEEQGIQCAP PPPAASQTGV PLTNLSHNCS ADGYIYQDSI ALPWKVLLVA LLALITLATT 70 80 90 100 110 120 LSNAFVIATV YRTRKLHTPA NYLIASLAVT DLLVSILVMP ISTMYTVTGR WTLGQVVCDF 130 140 150 160 170 180 WLSSDITCCT ASIMHLCVIA LDRYWAITDA VEYSAKRTPK RAAIMIVLVW VFSISISLPP 190 200 210 220 230 240 FFWRQAKAEE EMLDCFVNTD HVLYTVYSTV GAFYLPTLLL IALYGRIYVE ARSRILKQTP 250 260 270 280 290 300 NKTGKRLTRA QLITDSPGST SSVTSINSRA PDVPSESGSP VYVNQVKVRV SDALLEKKKL 310 320 330 340 350 360 MAARERKATK TLGIILGAFI VCWLPFFIIS LVMPICKDAC WFHMAIFDFF NWLGYLNSLI 370 380 NPIIYTMSNE DFKQAFHKLI RFKCAG

References

[1]	"Mouse 5HT1B serotonin receptor: cloning, functional expression, and localization in motor control centers." Maroteaux L., Saudou F., Amlaiky N., Boschert U., Plassat J.-L., Hen R. Proc. Natl. Acad. Sci. U.S.A. 89:3020-3024(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z11597 Genomic DNA. Translation: CAA77678.1. M85151 Genomic DNA. Translation: AAA83221.1.
IPI	IPI00119583.
PIR	A42688.
RefSeq	NP_034612.1. NM_010482.1.
UniGene	Mm.445308.
3D structure databases
ProteinModelPortal	P28334.
SMR	P28334. Positions 44-380.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000050898.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P28334.
Proteomic databases
PRIDE	P28334.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000051005; ENSMUSP00000050898; ENSMUSG00000049511.
GeneID	15551.
KEGG	mmu:15551.
Organism-specific databases
CTD	3351.
MGI	MGI:96274. Htr1b.
Phylogenomic databases
eggNOG	NOG249628.
HOGENOM	HOG000239242.
HOVERGEN	HBG106962.
InParanoid	P28334.
KO	K04153.
OMA	IALPWKV.
OrthoDB	EOG4R7VB6.
Gene expression databases
ArrayExpress	P28334.
Bgee	P28334.
CleanEx	MM_HTR1B.
Genevestigator	P28334.
GermOnline	ENSMUSG00000049511. Mus musculus.
Family and domain databases
InterPro	IPR002147. 5HT1B_rcpt. IPR002231. 5HT_rcpt. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00513. 5HT1BRECEPTR. PR01101. 5HTRECEPTOR. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P28334.
NextBio	288484.
SOURCE	Search...

Entry information

Entry name

5HT1B_MOUSE

Accession

Primary (citable) accession number: P28334

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	May 1, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program