Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P28334

- 5HT1B_MOUSE

UniProt

P28334 - 5HT1B_MOUSE

Protein

5-hydroxytryptamine receptor 1B

Gene

Htr1b

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Dec 1992)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various alkaloids and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity. Arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Regulates the release of 5-hydroxytryptamine, dopamine and acetylcholine in the brain, and thereby affects neural activity, nociceptive processing, pain perception, mood and behavior. Besides, plays a role in vasoconstriction of cerebral arteries.6 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei351 – 3511Important for species-specific agonist sensitivityBy similarity

    GO - Molecular functioni

    1. drug binding Source: UniProtKB
    2. serotonin binding Source: UniProtKB
    3. serotonin receptor activity Source: UniProtKB

    GO - Biological processi

    1. adenylate cyclase-inhibiting serotonin receptor signaling pathway Source: UniProtKB
    2. bone remodeling Source: MGI
    3. cellular response to alkaloid Source: UniProtKB
    4. cellular response to drug Source: UniProtKB
    5. cellular response to temperature stimulus Source: UniProtKB
    6. feeding behavior Source: InterPro
    7. G-protein coupled receptor internalization Source: UniProtKB
    8. negative regulation of serotonin secretion Source: UniProtKB
    9. protein kinase C-activating G-protein coupled receptor signaling pathway Source: UniProtKB
    10. regulation of behavior Source: InterPro
    11. response to drug Source: UniProtKB
    12. synaptic transmission Source: InterPro
    13. vasoconstriction Source: InterPro

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Keywords - Biological processi

    Behavior

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    5-hydroxytryptamine receptor 1B
    Short name:
    5-HT-1B
    Short name:
    5-HT1B
    Alternative name(s):
    Serotonin receptor 1B
    Gene namesi
    Name:Htr1b
    Synonyms:5ht1b
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:96274. Htr1b.

    Subcellular locationi

    Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. integral component of plasma membrane Source: UniProtKB
    3. plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Mutant male mice display increased aggressivity towards intruders. Treatment with a Htr1b agonist does not trigger increased locomotor behavior in mutant mice, contrary to what is observed with wild-type mice. Treatment with a Htr1b agonist does not inhibit 5-hydroxytryptamine release in the frontal cortex and hippocampus of mutant mice, contrary to what is observed with wild-type mice. Likewise, Htr1b agonists do not inhibit dopamine and acetylcholine release in brains from mutant mice.5 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 3863865-hydroxytryptamine receptor 1BPRO_0000068917Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi24 – 241N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi28 – 281N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi118 ↔ 195PROSITE-ProRule annotation
    Lipidationi384 – 3841S-palmitoyl cysteineSequence Analysis

    Post-translational modificationi

    Phosphorylated.By similarity
    Palmitoylated.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

    Proteomic databases

    PRIDEiP28334.

    PTM databases

    PhosphoSiteiP28334.

    Expressioni

    Tissue specificityi

    Predominantly expressed in striatum and Purkinje cells.1 Publication

    Gene expression databases

    BgeeiP28334.
    CleanExiMM_HTR1B.
    GenevestigatoriP28334.

    Interactioni

    Subunit structurei

    Homodimer. Heterodimer with HTR1D By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000050898.

    Structurei

    3D structure databases

    ProteinModelPortaliP28334.
    SMRiP28334. Positions 34-383.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 4545ExtracellularBy similarityAdd
    BLAST
    Topological domaini72 – 809CytoplasmicBy similarity
    Topological domaini107 – 11913ExtracellularBy similarityAdd
    BLAST
    Topological domaini142 – 16120CytoplasmicBy similarityAdd
    BLAST
    Topological domaini184 – 20118ExtracellularBy similarityAdd
    BLAST
    Topological domaini225 – 31187CytoplasmicBy similarityAdd
    BLAST
    Topological domaini333 – 34513ExtracellularBy similarityAdd
    BLAST
    Topological domaini368 – 38619CytoplasmicBy similarityAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei46 – 7126Helical; Name=1By similarityAdd
    BLAST
    Transmembranei81 – 10626Helical; Name=2By similarityAdd
    BLAST
    Transmembranei120 – 14122Helical; Name=3By similarityAdd
    BLAST
    Transmembranei162 – 18322Helical; Name=4By similarityAdd
    BLAST
    Transmembranei202 – 22423Helical; Name=5By similarityAdd
    BLAST
    Transmembranei312 – 33221Helical; Name=6By similarityAdd
    BLAST
    Transmembranei346 – 36722Helical; Name=7By similarityAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni121 – 13010Agonist bindingBy similarity
    Regioni323 – 3275Agonist bindingBy similarity

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi142 – 1443DRY motif; important for ligand-induced conformation changes and signalingBy similarity
    Motifi361 – 3655NPxxY motif; important for ligand-induced conformation changes and signalingBy similarity

    Domaini

    Ligands are bound in a hydrophobic pocket formed by the transmembrane helices.By similarity

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG249628.
    HOGENOMiHOG000239242.
    HOVERGENiHBG106962.
    InParanoidiP28334.
    KOiK04153.
    OMAiIALPWKV.
    OrthoDBiEOG7NCV3Q.
    PhylomeDBiP28334.
    TreeFamiTF316350.

    Family and domain databases

    Gene3Di1.20.1070.10. 2 hits.
    InterProiIPR002147. 5HT1B_rcpt.
    IPR002231. 5HT_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    [Graphical view]
    PANTHERiPTHR24247:SF16. PTHR24247:SF16. 1 hit.
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR00513. 5HT1BRECEPTR.
    PR01101. 5HTRECEPTOR.
    PR00237. GPCRRHODOPSN.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P28334-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEEQGIQCAP PPPAASQTGV PLTNLSHNCS ADGYIYQDSI ALPWKVLLVA    50
    LLALITLATT LSNAFVIATV YRTRKLHTPA NYLIASLAVT DLLVSILVMP 100
    ISTMYTVTGR WTLGQVVCDF WLSSDITCCT ASIMHLCVIA LDRYWAITDA 150
    VEYSAKRTPK RAAIMIVLVW VFSISISLPP FFWRQAKAEE EMLDCFVNTD 200
    HVLYTVYSTV GAFYLPTLLL IALYGRIYVE ARSRILKQTP NKTGKRLTRA 250
    QLITDSPGST SSVTSINSRA PDVPSESGSP VYVNQVKVRV SDALLEKKKL 300
    MAARERKATK TLGIILGAFI VCWLPFFIIS LVMPICKDAC WFHMAIFDFF 350
    NWLGYLNSLI NPIIYTMSNE DFKQAFHKLI RFKCAG 386
    Length:386
    Mass (Da):43,079
    Last modified:December 1, 1992 - v1
    Checksum:i58F70FBEA770C0B3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z11597 Genomic DNA. Translation: CAA77678.1.
    M85151 Genomic DNA. Translation: AAA83221.1.
    CCDSiCCDS23370.1.
    PIRiA42688.
    RefSeqiNP_034612.1. NM_010482.1.
    UniGeneiMm.445308.

    Genome annotation databases

    EnsembliENSMUST00000051005; ENSMUSP00000050898; ENSMUSG00000049511.
    ENSMUST00000183482; ENSMUSP00000139389; ENSMUSG00000049511.
    GeneIDi15551.
    KEGGimmu:15551.
    UCSCiuc009qvl.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z11597 Genomic DNA. Translation: CAA77678.1 .
    M85151 Genomic DNA. Translation: AAA83221.1 .
    CCDSi CCDS23370.1.
    PIRi A42688.
    RefSeqi NP_034612.1. NM_010482.1.
    UniGenei Mm.445308.

    3D structure databases

    ProteinModelPortali P28334.
    SMRi P28334. Positions 34-383.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000050898.

    Chemistry

    BindingDBi P28334.
    GuidetoPHARMACOLOGYi 2.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei P28334.

    Proteomic databases

    PRIDEi P28334.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000051005 ; ENSMUSP00000050898 ; ENSMUSG00000049511 .
    ENSMUST00000183482 ; ENSMUSP00000139389 ; ENSMUSG00000049511 .
    GeneIDi 15551.
    KEGGi mmu:15551.
    UCSCi uc009qvl.1. mouse.

    Organism-specific databases

    CTDi 3351.
    MGIi MGI:96274. Htr1b.

    Phylogenomic databases

    eggNOGi NOG249628.
    HOGENOMi HOG000239242.
    HOVERGENi HBG106962.
    InParanoidi P28334.
    KOi K04153.
    OMAi IALPWKV.
    OrthoDBi EOG7NCV3Q.
    PhylomeDBi P28334.
    TreeFami TF316350.

    Miscellaneous databases

    NextBioi 288484.
    PROi P28334.
    SOURCEi Search...

    Gene expression databases

    Bgeei P28334.
    CleanExi MM_HTR1B.
    Genevestigatori P28334.

    Family and domain databases

    Gene3Di 1.20.1070.10. 2 hits.
    InterProi IPR002147. 5HT1B_rcpt.
    IPR002231. 5HT_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    [Graphical view ]
    PANTHERi PTHR24247:SF16. PTHR24247:SF16. 1 hit.
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00513. 5HT1BRECEPTR.
    PR01101. 5HTRECEPTOR.
    PR00237. GPCRRHODOPSN.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mouse 5HT1B serotonin receptor: cloning, functional expression, and localization in motor control centers."
      Maroteaux L., Saudou F., Amlaiky N., Boschert U., Plassat J.-L., Hen R.
      Proc. Natl. Acad. Sci. U.S.A. 89:3020-3024(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    2. "Enhanced aggressive behavior in mice lacking 5-HT1B receptor."
      Saudou F., Amara D.A., Dierich A., LeMeur M., Ramboz S., Segu L., Buhot M.C., Hen R.
      Science 265:1875-1878(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE, FUNCTION.
    3. "Regulation of [3H]5-HT release in raphe, frontal cortex and hippocampus of 5-HT1B knock-out mice."
      Pineyro G., Castanon N., Hen R., Blier P.
      NeuroReport 7:353-359(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE, FUNCTION.
    4. "Regulation of serotonin release in the frontal cortex and ventral hippocampus of homozygous mice lacking 5-HT1B receptors: in vivo microdialysis studies."
      Trillat A.C., Malagie I., Scearce K., Pons D., Anmella M.C., Jacquot C., Hen R., Gardier A.M.
      J. Neurochem. 69:2019-2025(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE, FUNCTION.
    5. "5-HT1B receptors modulate release of [3H]dopamine from rat striatal synaptosomes: further evidence using 5-HT moduline, polyclonal 5-HT1B receptor antibodies and 5-HT1B receptor knock-out mice."
      Sarhan H., Grimaldi B., Hen R., Fillion G.
      Naunyn Schmiedebergs Arch. Pharmacol. 361:12-18(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE, FUNCTION.
    6. "Presynaptic serotonergic modulation of 5-HT and acetylcholine release in the hippocampus and the cortex of 5-HT1B-receptor knockout mice."
      Rutz S., Riegert C., Rothmaier A.K., Buhot M.C., Cassel J.C., Jackisch R.
      Brain Res. Bull. 70:81-93(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE, FUNCTION.

    Entry informationi

    Entry namei5HT1B_MOUSE
    AccessioniPrimary (citable) accession number: P28334
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    A residue in the 7th transmembrane region ('Thr-355' in human, Asn-351 in mouse and rat) is important for species-specific sensitivity to various agonists.By similarity

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3