P28332 (ADH6_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alcohol dehydrogenase 6
EC=1.1.1.1

Gene names

Name:

ADH6

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	368 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	An alcohol + NAD+ = an aldehyde or ketone + NADH.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Subcellular location	Cytoplasm.
Tissue specificity	Stomach and liver.
Miscellaneous	There are 7 different ADH's isozymes in human: three belongs to class-I: alpha, beta, and gamma, one to class-II: pi, one to class-III: chi, one to class-IV: ADH7 and one to class-V: ADH6.
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family. Class-V subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	Metal-binding NAD Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ethanol oxidation Inferred from direct assay PubMed 1755855. Source: UniProtKB response to ethanol Inferred from direct assay PubMed 1755855. Source: UniProtKB xenobiotic metabolic process Traceable author statement. Source: Reactome
Cellular_component	cytosol Traceable author statement. Source: Reactome
Molecular_function	alcohol dehydrogenase (NAD) activity Inferred from direct assay PubMed 1755855. Source: UniProtKB nucleotide binding Inferred from electronic annotation. Source: InterPro zinc ion binding Non-traceable author statement PubMed 1593644. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P28332-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P28332-2)

The sequence of this isoform differs from the canonical sequence as follows:
     368-368: W → CIRCILLL

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 368	368	Alcohol dehydrogenase 6		PRO_0000160687
Regions
	Nucleotide binding	200 – 205	6	NAD By similarity
	Nucleotide binding	293 – 295	3	NAD By similarity
Sites
	Metal binding	47	1	Zinc 1; catalytic By similarity
	Metal binding	69	1	Zinc 1; catalytic By similarity
	Metal binding	99	1	Zinc 2 By similarity
	Metal binding	102	1	Zinc 2 By similarity
	Metal binding	105	1	Zinc 2 By similarity
	Metal binding	113	1	Zinc 2 By similarity
	Metal binding	175	1	Zinc 1; catalytic By similarity
	Binding site	224	1	NAD By similarity
	Binding site	229	1	NAD By similarity
Natural variations
	Alternative sequence	368	1	W → CIRCILLL in isoform 2.		VSP_037702
	Natural variant	102	1	C → G. Ref.3 Corresponds to variant rs28720152 [ dbSNP | Ensembl ].		VAR_022655
	Natural variant	114	1	I → V. Ref.3 Corresponds to variant rs28720153 [ dbSNP | Ensembl ].		VAR_022656
	Natural variant	151	1	T → P. Corresponds to variant rs34582580 [ dbSNP | Ensembl ].		VAR_048198
Experimental info
	Sequence conflict	2	1	C → S in AAA35509. Ref.1
	Sequence conflict	2	1	C → S in BAG52607. Ref.2
	Sequence conflict	2	1	C → S in AAX44051. Ref.3
	Sequence conflict	266	1	C → R in BAG52607. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 28, 2009. Version 2. Checksum: C7E33043F6B4CA9B	FASTA	368	39,089
	10 20 30 40 50 60 MCTTGQVIRC KAAILWKPGA PFSIEEVEVA PPKAKEVRIK VVATGLCGTE MKVLGSKHLD 70 80 90 100 110 120 LLYPTILGHE GAGIVESIGE GVSTVKPGDK VITLFLPQCG ECTSCLNSEG NFCIQFKQSK 130 140 150 160 170 180 TQLMSDGTSR FTCKGKSIYH FGNTSTFCEY TVIKEISVAK IDAVAPLEKV CLISCGFSTG 190 200 210 220 230 240 FGAAINTAKV TPGSTCAVFG LGGVGLSVVM GCKAAGAARI IGVDVNKEKF KKAQELGATE 250 260 270 280 290 300 CLNPQDLKKP IQEVLFDMTD AGIDFCFEAI GNLDVLAAAL ASCNESYGVC VVVGVLPASV 310 320 330 340 350 360 QLKISGQLFF SGRSLKGSVF GGWKSRQHIP KLVADYMAEK LNLDPLITHT LNLDKINEAV ELMKTGKW
	Isoform 2 [UniParc]. Checksum: 53DA496C0DDE2CBA	FASTA	375	39,831
	10 20 30 40 50 60 MCTTGQVIRC KAAILWKPGA PFSIEEVEVA PPKAKEVRIK VVATGLCGTE MKVLGSKHLD 70 80 90 100 110 120 LLYPTILGHE GAGIVESIGE GVSTVKPGDK VITLFLPQCG ECTSCLNSEG NFCIQFKQSK 130 140 150 160 170 180 TQLMSDGTSR FTCKGKSIYH FGNTSTFCEY TVIKEISVAK IDAVAPLEKV CLISCGFSTG 190 200 210 220 230 240 FGAAINTAKV TPGSTCAVFG LGGVGLSVVM GCKAAGAARI IGVDVNKEKF KKAQELGATE 250 260 270 280 290 300 CLNPQDLKKP IQEVLFDMTD AGIDFCFEAI GNLDVLAAAL ASCNESYGVC VVVGVLPASV 310 320 330 340 350 360 QLKISGQLFF SGRSLKGSVF GGWKSRQHIP KLVADYMAEK LNLDPLITHT LNLDKINEAV 370 ELMKTGKCIR CILLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A human alcohol dehydrogenase gene (ADH6) encoding an additional class of isozyme." Yasunami M., Chen C.-S., Yoshida A. Proc. Natl. Acad. Sci. U.S.A. 88:7610-7614(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Liver.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Small intestine.
[3]	NIEHS SNPs program Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS GLY-102 AND VAL-114.
[4]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
+	Additional computationally mapped references.

Web resources

NIEHS-SNPs

Cross-references

Sequence databases
EMBL GenBank DDBJ	M84402 , M84403, M84404, M84405, M84406, M84407, M84408, M84409 Genomic DNA. Translation: AAA35509.1. AK092768 mRNA. Translation: BAG52607.1. AY962311 Genomic DNA. Translation: AAX44051.1. AP002026 Genomic DNA. No translation available.
IPI	IPI00292698. IPI00939339.
PIR	DEHUA6. A41274.
RefSeq	NP_000663.1. NM_000672.3. NP_001095940.1. NM_001102470.1.
UniGene	Hs.586161.
3D structure databases
ProteinModelPortal	P28332.
ModBase	Search...
Protein-protein interaction databases
IntAct	P28332. 8 interactions.
STRING	9606.ENSP00000378359.
PTM databases
PhosphoSite	P28332.
Polymorphism databases
DMDM	254763246.
Proteomic databases
PaxDb	P28332.
PRIDE	P28332.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000237653; ENSP00000237653; ENSG00000172955. ENST00000394899; ENSP00000378359; ENSG00000172955.
GeneID	130.
KEGG	hsa:130.
UCSC	uc003huo.2. human. uc003hup.4. human.
Organism-specific databases
CTD	130.
GeneCards	GC04M100123.
HGNC	HGNC:255. ADH6.
MIM	103735. gene.
neXtProt	NX_P28332.
PharmGKB	PA24576.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG1062.
HOGENOM	HOG000294674.
HOVERGEN	HBG000195.
KO	K13952.
OMA	TGKCIRC.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism.
Gene expression databases
ArrayExpress	P28332.
Bgee	P28332.
CleanEx	HS_ADH6.
Genevestigator	P28332.
GermOnline	ENSG00000172955. Homo sapiens.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR013149. ADH_C. IPR013154. ADH_GroES-like. IPR002085. ADH_SF_Zn-type. IPR002328. ADH_Zn_CS. IPR011032. GroES-like. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11695. PTHR11695. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. PF00107. ADH_zinc_N. 1 hit. [Graphical view]
SUPFAM	SSF50129. GroES_like. 1 hit.
PROSITE	PS00059. ADH_ZINC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P28332.
ChEMBL	CHEMBL4765.
DrugBank	DB01048. Abacavir. DB00157. NADH.
GenomeRNAi	130.
NextBio	517.
SOURCE	Search...

Entry information

Entry name

ADH6_HUMAN

Accession

Primary (citable) accession number: P28332
Secondary accession number(s): B3KS45, Q58F53

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	July 28, 2009
Last modified:	May 1, 2013
This is version 114 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.