Reviewed, UniProtKB/Swiss-Prot P28332 (ADH6_HUMAN)

Names and origin

Protein names

Recommended name:
    Alcohol dehydrogenase 6
    EC=1.1.1.1

Gene names

Name:

ADH6

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	368 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	An alcohol + NAD+ = an aldehyde or ketone + NADH.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Subcellular location	Cytoplasm.
Tissue specificity	Stomach and liver.
Miscellaneous	There are 7 different ADH's isozymes in human: three belongs to class-I: alpha, beta, and gamma, one to class-II: pi, one to class-III: chi, one to class-IV: ADH7 and one to class-V: ADH6.
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family. Class-V subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Ligand	Metal-binding NAD Zinc
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	ethanol oxidation Inferred from direct assay. Source: UniProtKB oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to ethanol Inferred from direct assay. Source: UniProtKB
Cellular component	cytosol Inferred from Experiment. Source: Reactome
Molecular function	alcohol dehydrogenase activity, zinc-dependent Inferred from Experiment. Source: Reactome electron carrier activity Inferred from direct assay. Source: UniProtKB zinc ion binding Traceable author statement. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 368	368	Alcohol dehydrogenase 6		PRO_0000160687
Regions
	Nucleotide binding	200 – 205	6	NAD By similarity
	Nucleotide binding	293 – 295	3	NAD By similarity
Sites
	Metal binding	47	1	Zinc 1; catalytic By similarity
	Metal binding	69	1	Zinc 1; catalytic By similarity
	Metal binding	99	1	Zinc 2 By similarity
	Metal binding	102	1	Zinc 2 By similarity
	Metal binding	105	1	Zinc 2 By similarity
	Metal binding	113	1	Zinc 2 By similarity
	Metal binding	175	1	Zinc 1; catalytic By similarity
	Binding site	224	1	NAD By similarity
	Binding site	229	1	NAD By similarity
Natural variations
	Natural variant	102	1	C → G: dbSNP rs28720152. Ref.2		VAR_022655
	Natural variant	114	1	I → V: dbSNP rs28720153. Ref.2		VAR_022656
	Natural variant	151	1	T → P: dbSNP rs34582580.		VAR_048198

Sequences

	Sequence		Length	Mass (Da)
	P28332-1 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: EC8E81E9571E6241	FASTA	368	39,073
	10 20 30 40 50 60 MSTTGQVIRC KAAILWKPGA PFSIEEVEVA PPKAKEVRIK VVATGLCGTE MKVLGSKHLD 70 80 90 100 110 120 LLYPTILGHE GAGIVESIGE GVSTVKPGDK VITLFLPQCG ECTSCLNSEG NFCIQFKQSK 130 140 150 160 170 180 TQLMSDGTSR FTCKGKSIYH FGNTSTFCEY TVIKEISVAK IDAVAPLEKV CLISCGFSTG 190 200 210 220 230 240 FGAAINTAKV TPGSTCAVFG LGGVGLSVVM GCKAAGAARI IGVDVNKEKF KKAQELGATE 250 260 270 280 290 300 CLNPQDLKKP IQEVLFDMTD AGIDFCFEAI GNLDVLAAAL ASCNESYGVC VVVGVLPASV 310 320 330 340 350 360 QLKISGQLFF SGRSLKGSVF GGWKSRQHIP KLVADYMAEK LNLDPLITHT LNLDKINEAV ELMKTGKW

References

[1]	"A human alcohol dehydrogenase gene (ADH6) encoding an additional class of isozyme." Yasunami M., Chen C.-S., Yoshida A. Proc. Natl. Acad. Sci. U.S.A. 88:7610-7614(1991) [PubMed: 1881901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Liver.
[2]	NIEHS SNPs program Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS GLY-102 AND VAL-114.
+	Additional computationally mapped references.

Web resources

NIEHS-SNPs

Cross-references

Sequence databases
EMBLGenBankDDBJ	M84402 , M84403, M84404, M84405, M84406, M84407, M84408, M84409 Genomic DNA. Translation: AAA35509.1. AY962311 Genomic DNA. Translation: AAX44051.1.
IPI	IPI00292698.
PIR	DEHUA6. A41274.
RefSeq	NP_000663.1.
UniGene	Hs.586161
3D structure databases
HSSP	HSSP built from PDB template 1HEU based on UniProtKB P00327.
ModBase	Search...
PTM databases
PhosphoSite	P28332.
Proteomic databases
PRIDE	P28332.
Genome annotation databases
Ensembl	ENSG00000172955. Homo sapiens. [Contig view]
GeneID	130.
KEGG	hsa:130.
Organism-specific databases
GeneCards	GC04M100373.
HGNC	HGNC:255. ADH6.
MIM	103735. gene.
PharmGKB	PA24576.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	P28332.
HOVERGEN	P28332.
Enzyme and pathway databases
BRENDA	1.1.1.1. 247.
Reactome	REACT_13433. Biological oxidations.
Gene expression databases
ArrayExpress	P28332.
Bgee	P28332.
CleanEx	HS_ADH6.
GermOnline	ENSG00000172955. Homo sapiens.
Family and domain databases
InterPro	IPR013154. ADH_GroES-like. IPR002085. ADH_SF_Zn. IPR013149. ADH_Zn-bd. IPR002328. ADH_Zn_CS. [Graphical view]
PANTHER	PTHR11695. ADH_Sf_Zn. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. PF00107. ADH_zinc_N. 1 hit. [Graphical view]
PROSITE	PS00059. ADH_ZINC. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
DrugBank	DB01048. Abacavir. DB00157. NADH.
NextBio	517.
SOURCE	Search...

Entry information

Entry name

ADH6_HUMAN

Accession

Primary (citable) accession number: P28332
Secondary accession number(s): Q58F53

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	June 16, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)