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Protein

Alcohol dehydrogenase 6

Gene

ADH6

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

An alcohol + NAD+ = an aldehyde or ketone + NADH.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi47 – 471Zinc 1; catalyticBy similarity
Metal bindingi69 – 691Zinc 1; catalyticBy similarity
Metal bindingi99 – 991Zinc 2By similarity
Metal bindingi102 – 1021Zinc 2By similarity
Metal bindingi105 – 1051Zinc 2By similarity
Metal bindingi113 – 1131Zinc 2By similarity
Metal bindingi175 – 1751Zinc 1; catalyticBy similarity
Binding sitei224 – 2241NADBy similarity
Binding sitei229 – 2291NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi200 – 2056NADBy similarity
Nucleotide bindingi293 – 2953NADBy similarity

GO - Molecular functioni

  • alcohol dehydrogenase (NAD) activity Source: UniProtKB
  • alcohol dehydrogenase activity, zinc-dependent Source: InterPro
  • ethanol binding Source: InterPro
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • ethanol oxidation Source: UniProtKB
  • response to ethanol Source: UniProtKB
  • small molecule metabolic process Source: Reactome
  • xenobiotic metabolic process Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Metal-binding, NAD, Zinc

Enzyme and pathway databases

ReactomeiREACT_34. Ethanol oxidation.

Names & Taxonomyi

Protein namesi
Recommended name:
Alcohol dehydrogenase 6 (EC:1.1.1.1)
Gene namesi
Name:ADH6
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:255. ADH6.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: Reactome
  • extracellular exosome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA24576.

Chemistry

DrugBankiDB01048. Abacavir.

Polymorphism and mutation databases

BioMutaiADH6.
DMDMi254763246.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 368368Alcohol dehydrogenase 6PRO_0000160687Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei23 – 231Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP28332.
PaxDbiP28332.
PRIDEiP28332.

PTM databases

PhosphoSiteiP28332.

Expressioni

Tissue specificityi

Stomach and liver.

Gene expression databases

BgeeiP28332.
CleanExiHS_ADH6.
ExpressionAtlasiP28332. baseline and differential.
GenevisibleiP28332. HS.

Interactioni

Protein-protein interaction databases

BioGridi106642. 8 interactions.
IntActiP28332. 8 interactions.
STRINGi9606.ENSP00000378359.

Structurei

3D structure databases

ProteinModelPortaliP28332.
SMRiP28332. Positions 3-367.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1062.
HOGENOMiHOG000294674.
HOVERGENiHBG000195.
InParanoidiP28332.
KOiK13952.
PhylomeDBiP28332.
TreeFamiTF300429.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR028633. ADH6.
IPR013149. ADH_C.
IPR013154. ADH_GroES-like.
IPR002085. ADH_SF_Zn-type.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PTHR11695:SF307. PTHR11695:SF307. 1 hit.
PfamiPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 2 hits.
PROSITEiPS00059. ADH_ZINC. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P28332-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MCTTGQVIRC KAAILWKPGA PFSIEEVEVA PPKAKEVRIK VVATGLCGTE
60 70 80 90 100
MKVLGSKHLD LLYPTILGHE GAGIVESIGE GVSTVKPGDK VITLFLPQCG
110 120 130 140 150
ECTSCLNSEG NFCIQFKQSK TQLMSDGTSR FTCKGKSIYH FGNTSTFCEY
160 170 180 190 200
TVIKEISVAK IDAVAPLEKV CLISCGFSTG FGAAINTAKV TPGSTCAVFG
210 220 230 240 250
LGGVGLSVVM GCKAAGAARI IGVDVNKEKF KKAQELGATE CLNPQDLKKP
260 270 280 290 300
IQEVLFDMTD AGIDFCFEAI GNLDVLAAAL ASCNESYGVC VVVGVLPASV
310 320 330 340 350
QLKISGQLFF SGRSLKGSVF GGWKSRQHIP KLVADYMAEK LNLDPLITHT
360
LNLDKINEAV ELMKTGKW
Length:368
Mass (Da):39,089
Last modified:July 28, 2009 - v2
Checksum:iC7E33043F6B4CA9B
GO
Isoform 2 (identifier: P28332-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     368-368: W → CIRCILLL

Show »
Length:375
Mass (Da):39,831
Checksum:i53DA496C0DDE2CBA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 21C → S in AAA35509 (PubMed:1881901).Curated
Sequence conflicti2 – 21C → S in BAG52607 (PubMed:14702039).Curated
Sequence conflicti2 – 21C → S in AAX44051 (Ref. 3) Curated
Sequence conflicti266 – 2661C → R in BAG52607 (PubMed:14702039).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti102 – 1021C → G.1 Publication
Corresponds to variant rs28720152 [ dbSNP | Ensembl ].
VAR_022655
Natural varianti114 – 1141I → V.1 Publication
Corresponds to variant rs28720153 [ dbSNP | Ensembl ].
VAR_022656
Natural varianti151 – 1511T → P.
Corresponds to variant rs34582580 [ dbSNP | Ensembl ].
VAR_048198

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei368 – 3681W → CIRCILLL in isoform 2. 1 PublicationVSP_037702

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84402
, M84403, M84404, M84405, M84406, M84407, M84408, M84409 Genomic DNA. Translation: AAA35509.1.
AK092768 mRNA. Translation: BAG52607.1.
AY962311 Genomic DNA. Translation: AAX44051.1.
AP002026 Genomic DNA. No translation available.
CCDSiCCDS3647.1. [P28332-1]
CCDS43255.1. [P28332-2]
PIRiA41274. DEHUA6.
RefSeqiNP_000663.1. NM_000672.3.
NP_001095940.1. NM_001102470.1.
UniGeneiHs.586161.

Genome annotation databases

EnsembliENST00000237653; ENSP00000237653; ENSG00000172955.
GeneIDi130.
KEGGihsa:130.
UCSCiuc003huo.2. human. [P28332-2]
uc003hup.4. human. [P28332-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84402
, M84403, M84404, M84405, M84406, M84407, M84408, M84409 Genomic DNA. Translation: AAA35509.1.
AK092768 mRNA. Translation: BAG52607.1.
AY962311 Genomic DNA. Translation: AAX44051.1.
AP002026 Genomic DNA. No translation available.
CCDSiCCDS3647.1. [P28332-1]
CCDS43255.1. [P28332-2]
PIRiA41274. DEHUA6.
RefSeqiNP_000663.1. NM_000672.3.
NP_001095940.1. NM_001102470.1.
UniGeneiHs.586161.

3D structure databases

ProteinModelPortaliP28332.
SMRiP28332. Positions 3-367.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106642. 8 interactions.
IntActiP28332. 8 interactions.
STRINGi9606.ENSP00000378359.

Chemistry

ChEMBLiCHEMBL2096668.
DrugBankiDB01048. Abacavir.

PTM databases

PhosphoSiteiP28332.

Polymorphism and mutation databases

BioMutaiADH6.
DMDMi254763246.

Proteomic databases

MaxQBiP28332.
PaxDbiP28332.
PRIDEiP28332.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000237653; ENSP00000237653; ENSG00000172955.
GeneIDi130.
KEGGihsa:130.
UCSCiuc003huo.2. human. [P28332-2]
uc003hup.4. human. [P28332-1]

Organism-specific databases

CTDi130.
GeneCardsiGC04M100123.
HGNCiHGNC:255. ADH6.
MIMi103735. gene.
neXtProtiNX_P28332.
PharmGKBiPA24576.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG1062.
HOGENOMiHOG000294674.
HOVERGENiHBG000195.
InParanoidiP28332.
KOiK13952.
PhylomeDBiP28332.
TreeFamiTF300429.

Enzyme and pathway databases

ReactomeiREACT_34. Ethanol oxidation.

Miscellaneous databases

GeneWikiiADH6.
GenomeRNAii130.
NextBioi517.
PROiP28332.
SOURCEiSearch...

Gene expression databases

BgeeiP28332.
CleanExiHS_ADH6.
ExpressionAtlasiP28332. baseline and differential.
GenevisibleiP28332. HS.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR028633. ADH6.
IPR013149. ADH_C.
IPR013154. ADH_GroES-like.
IPR002085. ADH_SF_Zn-type.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PTHR11695:SF307. PTHR11695:SF307. 1 hit.
PfamiPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 2 hits.
PROSITEiPS00059. ADH_ZINC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A human alcohol dehydrogenase gene (ADH6) encoding an additional class of isozyme."
    Yasunami M., Chen C.-S., Yoshida A.
    Proc. Natl. Acad. Sci. U.S.A. 88:7610-7614(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Liver.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Small intestine.
  3. NIEHS SNPs program
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS GLY-102 AND VAL-114.
  4. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-23, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiADH6_HUMAN
AccessioniPrimary (citable) accession number: P28332
Secondary accession number(s): B3KS45, Q58F53
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: July 28, 2009
Last modified: July 22, 2015
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

There are 7 different ADH's isozymes in human: three belongs to class-I: alpha, beta, and gamma, one to class-II: pi, one to class-III: chi, one to class-IV: ADH7 and one to class-V: ADH6.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.