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P28324

- ELK4_HUMAN

UniProt

P28324 - ELK4_HUMAN

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Protein

ETS domain-containing protein Elk-4

Gene
ELK4, SAP1
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in both transcriptional activation and repression. Interaction with SIRT7 leads to recruitment and stabilization of SIRT7 at promoters, followed by deacetylation of histone H3 at 'Lys-18' (H3K18Ac) and subsequent transcription repression. Forms a ternary complex with the serum response factor (SRF). Requires DNA-bound SRF for ternary complex formation and makes extensive DNA contacts to the 5'side of SRF, but does not bind DNA autonomously.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi5 – 8581ETSAdd
BLAST

GO - Molecular functioni

  1. chromatin binding Source: UniProtKB
  2. core promoter binding Source: UniProtKB
  3. DNA binding Source: ProtInc
  4. protein binding Source: UniProtKB
  5. sequence-specific DNA binding Source: InterPro
  6. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: RefGenome
  7. sequence-specific DNA binding transcription factor activity Source: ProtInc
  8. transcription cofactor activity Source: ProtInc

GO - Biological processi

  1. cell differentiation Source: RefGenome
  2. histone H3 deacetylation Source: UniProtKB
  3. negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  4. transcription from RNA polymerase II promoter Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

SignaLinkiP28324.

Names & Taxonomyi

Protein namesi
Recommended name:
ETS domain-containing protein Elk-4
Alternative name(s):
Serum response factor accessory protein 1
Short name:
SAP-1
Short name:
SRF accessory protein 1
Gene namesi
Name:ELK4
Synonyms:SAP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 1

Organism-specific databases

HGNCiHGNC:3326. ELK4.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: HPA
  2. nucleus Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA27753.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 431431ETS domain-containing protein Elk-4PRO_0000204099Add
BLAST

Proteomic databases

PaxDbiP28324.
PRIDEiP28324.

PTM databases

PhosphoSiteiP28324.

Expressioni

Gene expression databases

ArrayExpressiP28324.
BgeeiP28324.
CleanExiHS_ELK4.
GenevestigatoriP28324.

Organism-specific databases

HPAiHPA028863.

Interactioni

Subunit structurei

Interacts with SIRT7.1 Publication

Protein-protein interaction databases

BioGridi108320. 5 interactions.
DIPiDIP-59907N.
STRINGi9606.ENSP00000350681.

Structurei

Secondary structure

1
431
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 148
Helixi18 – 203
Turni21 – 233
Beta strandi24 – 263
Beta strandi28 – 347
Helixi38 – 4912
Helixi56 – 6914
Beta strandi71 – 744
Beta strandi76 – 783
Beta strandi81 – 844
Turni85 – 884
Helixi89 – 913
Helixi138 – 1436
Beta strandi144 – 1529
Helixi153 – 1553

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BC7X-ray2.01C1-93[»]
1BC8X-ray1.93C1-93[»]
1HBXX-ray3.15G/H2-156[»]
1K6OX-ray3.19A1-93[»]
ProteinModelPortaliP28324.
SMRiP28324. Positions 1-156.

Miscellaneous databases

EvolutionaryTraceiP28324.

Family & Domainsi

Sequence similaritiesi

Belongs to the ETS family.

Phylogenomic databases

eggNOGiNOG317055.
HOVERGENiHBG004344.
InParanoidiP28324.
KOiK04376.
OMAiICWTSNN.
OrthoDBiEOG7NPFTD.
PhylomeDBiP28324.
TreeFamiTF317732.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000418. Ets_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00178. Ets. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
[Graphical view]
PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P28324-1) [UniParc]FASTAAdd to Basket

Also known as: SAP-1A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MDSAITLWQF LLQLLQKPQN KHMICWTSND GQFKLLQAEE VARLWGIRKN    50
KPNMNYDKLS RALRYYYVKN IIKKVNGQKF VYKFVSYPEI LNMDPMTVGR 100
IEGDCESLNF SEVSSSSKDV ENGGKDKPPQ PGAKTSSRND YIHSGLYSSF 150
TLNSLNSSNV KLFKLIKTEN PAEKLAEKKS PQEPTPSVIK FVTTPSKKPP 200
VEPVAATISI GPSISPSSEE TIQALETLVS PKLPSLEAPT SASNVMTAFA 250
TTPPISSIPP LQEPPRTPSP PLSSHPDIDT DIDSVASQPM ELPENLSLEP 300
KDQDSVLLEK DKVNNSSRSK KPKGLELAPT LVITSSDPSP LGILSPSLPT 350
ASLTPAFFSQ TPIILTPSPL LSSIHFWSTL SPVAPLSPAR LQGANTLFQF 400
PSVLNSHGPF TLSGLDGPST PGPFSPDLQK T 431
Length:431
Mass (Da):46,900
Last modified:January 24, 2001 - v3
Checksum:i80EE3E69995C7A7D
GO
Isoform 2 (identifier: P28324-2) [UniParc]FASTAAdd to Basket

Also known as: SAP-1B

The sequence of this isoform differs from the canonical sequence as follows:
     361-431: TPIILTPSPL...GPFSPDLQKT → VACSLFMVSP...VLERLCVTVM

Show »
Length:405
Mass (Da):44,674
Checksum:i13DC31EA9C6D5B89
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei361 – 43171TPIIL…DLQKT → VACSLFMVSPLLSFICPFKQ IQNLYTQVCFLLLRFVLERL CVTVM in isoform 2. VSP_001468Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti326 – 3261E → G in AAA03631. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M85165 mRNA. Translation: AAA03631.1.
M85164 mRNA. Translation: AAA03632.1.
CH471067 Genomic DNA. Translation: EAW91570.1.
BC063676 mRNA. Translation: AAH63676.1.
CCDSiCCDS1456.1. [P28324-1]
CCDS1457.1. [P28324-2]
PIRiA42093.
A53012.
B42093.
RefSeqiNP_001964.2. NM_001973.3. [P28324-1]
NP_068567.1. NM_021795.2. [P28324-2]
XP_005245007.1. XM_005244950.2. [P28324-1]
XP_005245008.1. XM_005244951.2. [P28324-1]
UniGeneiHs.497520.
Hs.602654.

Genome annotation databases

EnsembliENST00000289703; ENSP00000289703; ENSG00000158711. [P28324-2]
ENST00000357992; ENSP00000350681; ENSG00000158711. [P28324-1]
GeneIDi2005.
KEGGihsa:2005.
UCSCiuc001hcy.2. human. [P28324-1]
uc001hcz.3. human. [P28324-2]

Polymorphism databases

DMDMi12585557.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M85165 mRNA. Translation: AAA03631.1 .
M85164 mRNA. Translation: AAA03632.1 .
CH471067 Genomic DNA. Translation: EAW91570.1 .
BC063676 mRNA. Translation: AAH63676.1 .
CCDSi CCDS1456.1. [P28324-1 ]
CCDS1457.1. [P28324-2 ]
PIRi A42093.
A53012.
B42093.
RefSeqi NP_001964.2. NM_001973.3. [P28324-1 ]
NP_068567.1. NM_021795.2. [P28324-2 ]
XP_005245007.1. XM_005244950.2. [P28324-1 ]
XP_005245008.1. XM_005244951.2. [P28324-1 ]
UniGenei Hs.497520.
Hs.602654.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1BC7 X-ray 2.01 C 1-93 [» ]
1BC8 X-ray 1.93 C 1-93 [» ]
1HBX X-ray 3.15 G/H 2-156 [» ]
1K6O X-ray 3.19 A 1-93 [» ]
ProteinModelPortali P28324.
SMRi P28324. Positions 1-156.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 108320. 5 interactions.
DIPi DIP-59907N.
STRINGi 9606.ENSP00000350681.

PTM databases

PhosphoSitei P28324.

Polymorphism databases

DMDMi 12585557.

Proteomic databases

PaxDbi P28324.
PRIDEi P28324.

Protocols and materials databases

DNASUi 2005.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000289703 ; ENSP00000289703 ; ENSG00000158711 . [P28324-2 ]
ENST00000357992 ; ENSP00000350681 ; ENSG00000158711 . [P28324-1 ]
GeneIDi 2005.
KEGGi hsa:2005.
UCSCi uc001hcy.2. human. [P28324-1 ]
uc001hcz.3. human. [P28324-2 ]

Organism-specific databases

CTDi 2005.
GeneCardsi GC01M205577.
HGNCi HGNC:3326. ELK4.
HPAi HPA028863.
MIMi 600246. gene.
neXtProti NX_P28324.
PharmGKBi PA27753.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG317055.
HOVERGENi HBG004344.
InParanoidi P28324.
KOi K04376.
OMAi ICWTSNN.
OrthoDBi EOG7NPFTD.
PhylomeDBi P28324.
TreeFami TF317732.

Enzyme and pathway databases

SignaLinki P28324.

Miscellaneous databases

EvolutionaryTracei P28324.
GeneWikii ELK4.
GenomeRNAii 2005.
NextBioi 8113.
PROi P28324.
SOURCEi Search...

Gene expression databases

ArrayExpressi P28324.
Bgeei P28324.
CleanExi HS_ELK4.
Genevestigatori P28324.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
InterProi IPR000418. Ets_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF00178. Ets. 1 hit.
[Graphical view ]
PRINTSi PR00454. ETSDOMAIN.
SMARTi SM00413. ETS. 1 hit.
[Graphical view ]
PROSITEi PS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of SAP-1, a protein recruited by serum response factor to the c-fos serum response element."
    Dalton S., Treisman R.
    Cell 68:597-612(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
  2. "Characterization of SAP-1, a protein recruited by serum response factor to the c-fos serum response element."
    Dalton S., Treisman R.
    Cell 76:411-411(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Lymph.
  5. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  6. "Structures of SAP-1 bound to DNA targets from the E74 and c-fos promoters: insights into DNA sequence discrimination by Ets proteins."
    Mo Y., Vaessen B., Johnston K., Marmorstein R.
    Mol. Cell 2:201-212(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.01 ANGSTROMS) OF 1-93.
  7. Cited for: FUNCTION, INTERACTION WITH SIRT7.

Entry informationi

Entry nameiELK4_HUMAN
AccessioniPrimary (citable) accession number: P28324
Secondary accession number(s): P28323, Q6GSJ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: January 24, 2001
Last modified: July 9, 2014
This is version 141 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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