Reviewed, UniProtKB/Swiss-Prot P28324 (ELK4_HUMAN)

Names and origin

Protein names

Recommended name:
    ETS domain-containing protein Elk-4
Alternative name(s):
    Serum response factor accessory protein 1
      Short name=SRF accessory protein 1
      Short name=SAP-1

Gene names

Name:	ELK4
Synonyms:	SAP1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	431 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Forms a ternary complex with the serum response factor (SRF). Requires DNA-bound SRF for ternary complex formation and makes extensive DNA contacts to the 5'side of SRF, but does not bind DNA autonomously.
Subcellular location	Nucleus.
Sequence similarities	Belongs to the ETS family. Contains 1 ETS DNA-binding domain.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro transcription cofactor activity Traceable author statement. Source: ProtInc transcription factor activity Non-traceable author statement. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P28324-1)

Also known as: SAP-1A;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P28324-2)

Also known as: SAP-1B;

The sequence of this isoform differs from the canonical sequence as follows:
     361-431: TPIILTPSPL...GPFSPDLQKT → VACSLFMVSP...VLERLCVTVM

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 431

431

ETS domain-containing protein Elk-4

PRO_0000204099

Regions

DNA binding

5 – 85

81

ETS

Natural variations

Alternative sequence

361 – 431

71

TPIIL…DLQKT → VACSLFMVSPLLSFICPFKQ IQNLYTQVCFLLLRFVLERL CVTVM in isoform 2.

VSP_001468

Experimental info

Sequence conflict

326

1

E → G in AAA03631. Ref.1

Secondary structure

1

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431

Helix Strand Turn

Details...

Helix

7 – 16

10

Helix

18 – 20

3

Turn

21 – 23

3

Beta strand

24 – 26

3

Beta strand

28 – 35

8

Helix

38 – 49

12

Helix

56 – 64

9

Helix

66 – 69

4

Beta strand

71 – 74

4

Beta strand

81 – 86

6

Turn

87 – 92

6

Helix

138 – 143

6

Beta strand

144 – 152

9

Helix

153 – 155

3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (SAP-1A) [UniParc]. Last modified January 24, 2001. Version 3. Checksum: 80EE3E69995C7A7D	FASTA	431	46,900
	10 20 30 40 50 60 MDSAITLWQF LLQLLQKPQN KHMICWTSND GQFKLLQAEE VARLWGIRKN KPNMNYDKLS 70 80 90 100 110 120 RALRYYYVKN IIKKVNGQKF VYKFVSYPEI LNMDPMTVGR IEGDCESLNF SEVSSSSKDV 130 140 150 160 170 180 ENGGKDKPPQ PGAKTSSRND YIHSGLYSSF TLNSLNSSNV KLFKLIKTEN PAEKLAEKKS 190 200 210 220 230 240 PQEPTPSVIK FVTTPSKKPP VEPVAATISI GPSISPSSEE TIQALETLVS PKLPSLEAPT 250 260 270 280 290 300 SASNVMTAFA TTPPISSIPP LQEPPRTPSP PLSSHPDIDT DIDSVASQPM ELPENLSLEP 310 320 330 340 350 360 KDQDSVLLEK DKVNNSSRSK KPKGLELAPT LVITSSDPSP LGILSPSLPT ASLTPAFFSQ 370 380 390 400 410 420 TPIILTPSPL LSSIHFWSTL SPVAPLSPAR LQGANTLFQF PSVLNSHGPF TLSGLDGPST 430 PGPFSPDLQK T
	Isoform 2 (SAP-1B). Checksum: 13DC31EA9C6D5B89	FASTA	405	44,674
	10 20 30 40 50 60 MDSAITLWQF LLQLLQKPQN KHMICWTSND GQFKLLQAEE VARLWGIRKN KPNMNYDKLS 70 80 90 100 110 120 RALRYYYVKN IIKKVNGQKF VYKFVSYPEI LNMDPMTVGR IEGDCESLNF SEVSSSSKDV 130 140 150 160 170 180 ENGGKDKPPQ PGAKTSSRND YIHSGLYSSF TLNSLNSSNV KLFKLIKTEN PAEKLAEKKS 190 200 210 220 230 240 PQEPTPSVIK FVTTPSKKPP VEPVAATISI GPSISPSSEE TIQALETLVS PKLPSLEAPT 250 260 270 280 290 300 SASNVMTAFA TTPPISSIPP LQEPPRTPSP PLSSHPDIDT DIDSVASQPM ELPENLSLEP 310 320 330 340 350 360 KDQDSVLLEK DKVNNSSRSK KPKGLELAPT LVITSSDPSP LGILSPSLPT ASLTPAFFSQ 370 380 390 400 VACSLFMVSP LLSFICPFKQ IQNLYTQVCF LLLRFVLERL CVTVM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of SAP-1, a protein recruited by serum response factor to the c-fos serum response element." Dalton S., Treisman R. Cell 68:597-612(1992) [PubMed: 1339307] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
[2]	"Characterization of SAP-1, a protein recruited by serum response factor to the c-fos serum response element." Dalton S., Treisman R. Cell 76:411-411(1994) [PubMed: 8293474] [Abstract] Cited for: SEQUENCE REVISION.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Lymph.
[5]	"Structures of SAP-1 bound to DNA targets from the E74 and c-fos promoters: insights into DNA sequence discrimination by Ets proteins." Mo Y., Vaessen B., Johnston K., Marmorstein R. Mol. Cell 2:201-212(1998) [PubMed: 9734357] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.01 ANGSTROMS) OF 1-93.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M85165 mRNA. Translation: AAA03631.1.
M85164 mRNA. Translation: AAA03632.1.
CH471067 Genomic DNA. Translation: EAW91570.1.
BC063676 mRNA. Translation: AAH63676.1.

IPI

IPI00002849.
IPI00220318.

PIR

A42093.
A53012.
B42093.

RefSeq

NP_001964.2.
NP_068567.1.

UniGene

Hs.497520
Hs.602654

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BC7	X-ray	2.01	C	1-93	[»]
1BC8	X-ray	1.93	C	1-93	[»]
1HBX	X-ray	3.15	G/H	2-156	[»]
1K6O	X-ray	3.19	A	1-93	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

P28324.

PTM databases

PhosphoSite

P28324.

Proteomic databases

PRIDE

P28324.

Genome annotation databases

Ensembl

ENST00000357992; ENSP00000350681; ENSG00000158711; Homo sapiens. [Genome view]

GeneID

2005.

KEGG

hsa:2005.

UCSC

uc001hcy.1. human.

Organism-specific databases

CTD

2005.

GeneCards

GC01M203833.

H-InvDB

HIX0028754.

HGNC

HGNC:3326. ELK4.

MIM

600246. gene.

PharmGKB

PA27753.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG10237.

HOVERGEN

P28324.

InParanoid

P28324.

OMA

AFFSQTP.

OrthoDB

EOG9RZ1JN.

PhylomeDB

P28324.

Enzyme and pathway databases

Pathway_Interaction_DB

p38alphabetadownstreampathway. Signaling mediated by p38-alpha and p38-beta.

Gene expression databases

ArrayExpress

P28324.

Bgee

P28324.

CleanEx

HS_ELK4.

Genevestigator

P28324.

GermOnline

ENSG00000158711. Homo sapiens.

Family and domain databases

InterPro

IPR000418. Ets.
IPR011991. WHTH_trsnscrt_rep_DNA-bd.
[Graphical view]

Gene3D

G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.

Pfam

PF00178. Ets. 1 hit.
[Graphical view]

PRINTS

PR00454. ETSDOMAIN.

SMART

SM00413. ETS. 1 hit.
[Graphical view]

PROSITE

PS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

8113.

SOURCE

Search...

Entry information

Entry name

ELK4_HUMAN

Accession

Primary (citable) accession number: P28324
Secondary accession number(s): P28323, Q6GSJ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	January 24, 2001
Last modified:	February 9, 2010
This is version 97 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.