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Protein

ETS domain-containing protein Elk-4

Gene

ELK4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in both transcriptional activation and repression. Interaction with SIRT7 leads to recruitment and stabilization of SIRT7 at promoters, followed by deacetylation of histone H3 at 'Lys-18' (H3K18Ac) and subsequent transcription repression. Forms a ternary complex with the serum response factor (SRF). Requires DNA-bound SRF for ternary complex formation and makes extensive DNA contacts to the 5'side of SRF, but does not bind DNA autonomously.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi5 – 85ETSPROSITE-ProRule annotationAdd BLAST81

GO - Molecular functioni

  • chromatin binding Source: UniProtKB
  • core promoter binding Source: UniProtKB
  • DNA binding Source: ProtInc
  • DNA binding transcription factor activity Source: ProtInc
  • RNA polymerase II proximal promoter sequence-specific DNA binding Source: NTNU_SB
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: NTNU_SB
  • transcriptional activator activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: NTNU_SB
  • transcription cofactor activity Source: ProtInc

GO - Biological processi

  • cell differentiation Source: GO_Central
  • histone H3 deacetylation Source: UniProtKB
  • negative regulation of transcription by RNA polymerase II Source: UniProtKB
  • positive regulation of transcription by RNA polymerase II Source: NTNU_SB

Keywordsi

Molecular functionActivator, DNA-binding, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

SignaLinkiP28324
SIGNORiP28324

Names & Taxonomyi

Protein namesi
Recommended name:
ETS domain-containing protein Elk-4
Alternative name(s):
Serum response factor accessory protein 1
Short name:
SAP-1
Short name:
SRF accessory protein 1
Gene namesi
Name:ELK4
Synonyms:SAP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000158711.13
HGNCiHGNC:3326 ELK4
MIMi600246 gene
neXtProtiNX_P28324

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi2005
OpenTargetsiENSG00000158711
PharmGKBiPA27753

Polymorphism and mutation databases

BioMutaiELK4
DMDMi12585557

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002040991 – 431ETS domain-containing protein Elk-4Add BLAST431

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki167Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei180PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP28324
PaxDbiP28324
PeptideAtlasiP28324
PRIDEiP28324

PTM databases

iPTMnetiP28324
PhosphoSitePlusiP28324

Expressioni

Gene expression databases

BgeeiENSG00000158711
CleanExiHS_ELK4
ExpressionAtlasiP28324 baseline and differential
GenevisibleiP28324 HS

Organism-specific databases

HPAiHPA028863

Interactioni

Subunit structurei

Interacts with SIRT7.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SIRT7Q9NRC83EBI-11277718,EBI-716046

Protein-protein interaction databases

BioGridi108320, 11 interactors
CORUMiP28324
DIPiDIP-59907N
IntActiP28324, 3 interactors
STRINGi9606.ENSP00000350681

Structurei

Secondary structure

1431
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 14Combined sources8
Helixi18 – 20Combined sources3
Turni21 – 23Combined sources3
Beta strandi24 – 26Combined sources3
Beta strandi28 – 34Combined sources7
Helixi38 – 49Combined sources12
Helixi56 – 69Combined sources14
Beta strandi71 – 74Combined sources4
Beta strandi76 – 78Combined sources3
Beta strandi81 – 84Combined sources4
Turni85 – 88Combined sources4
Helixi89 – 91Combined sources3
Helixi138 – 143Combined sources6
Beta strandi144 – 152Combined sources9
Helixi153 – 155Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BC7X-ray2.01C1-93[»]
1BC8X-ray1.93C1-93[»]
1HBXX-ray3.15G/H2-156[»]
1K6OX-ray3.19A1-93[»]
ProteinModelPortaliP28324
SMRiP28324
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP28324

Family & Domainsi

Sequence similaritiesi

Belongs to the ETS family.Curated

Phylogenomic databases

eggNOGiKOG3806 Eukaryota
ENOG410Z0ZF LUCA
GeneTreeiENSGT00760000118907
HOVERGENiHBG004344
InParanoidiP28324
KOiK04376
OMAiICWTSNN
OrthoDBiEOG091G0COO
PhylomeDBiP28324
TreeFamiTF317732

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR000418 Ets_dom
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00178 Ets, 1 hit
PRINTSiPR00454 ETSDOMAIN
SMARTiView protein in SMART
SM00413 ETS, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS00345 ETS_DOMAIN_1, 1 hit
PS00346 ETS_DOMAIN_2, 1 hit
PS50061 ETS_DOMAIN_3, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P28324-1) [UniParc]FASTAAdd to basket
Also known as: SAP-1A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDSAITLWQF LLQLLQKPQN KHMICWTSND GQFKLLQAEE VARLWGIRKN
60 70 80 90 100
KPNMNYDKLS RALRYYYVKN IIKKVNGQKF VYKFVSYPEI LNMDPMTVGR
110 120 130 140 150
IEGDCESLNF SEVSSSSKDV ENGGKDKPPQ PGAKTSSRND YIHSGLYSSF
160 170 180 190 200
TLNSLNSSNV KLFKLIKTEN PAEKLAEKKS PQEPTPSVIK FVTTPSKKPP
210 220 230 240 250
VEPVAATISI GPSISPSSEE TIQALETLVS PKLPSLEAPT SASNVMTAFA
260 270 280 290 300
TTPPISSIPP LQEPPRTPSP PLSSHPDIDT DIDSVASQPM ELPENLSLEP
310 320 330 340 350
KDQDSVLLEK DKVNNSSRSK KPKGLELAPT LVITSSDPSP LGILSPSLPT
360 370 380 390 400
ASLTPAFFSQ TPIILTPSPL LSSIHFWSTL SPVAPLSPAR LQGANTLFQF
410 420 430
PSVLNSHGPF TLSGLDGPST PGPFSPDLQK T
Length:431
Mass (Da):46,900
Last modified:January 24, 2001 - v3
Checksum:i80EE3E69995C7A7D
GO
Isoform 2 (identifier: P28324-2) [UniParc]FASTAAdd to basket
Also known as: SAP-1B

The sequence of this isoform differs from the canonical sequence as follows:
     361-431: TPIILTPSPL...GPFSPDLQKT → VACSLFMVSP...VLERLCVTVM

Show »
Length:405
Mass (Da):44,674
Checksum:i13DC31EA9C6D5B89
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti326E → G in AAA03631 (PubMed:1339307).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_001468361 – 431TPIIL…DLQKT → VACSLFMVSPLLSFICPFKQ IQNLYTQVCFLLLRFVLERL CVTVM in isoform 2. 1 PublicationAdd BLAST71

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M85165 mRNA Translation: AAA03631.1
M85164 mRNA Translation: AAA03632.1
CH471067 Genomic DNA Translation: EAW91570.1
BC063676 mRNA Translation: AAH63676.1
CCDSiCCDS1456.1 [P28324-1]
CCDS1457.1 [P28324-2]
PIRiA42093
A53012
B42093
RefSeqiNP_001964.2, NM_001973.3 [P28324-1]
NP_068567.1, NM_021795.2 [P28324-2]
XP_005245007.1, XM_005244950.4 [P28324-1]
XP_005245008.1, XM_005244951.4 [P28324-1]
UniGeneiHs.497520
Hs.602654

Genome annotation databases

EnsembliENST00000289703; ENSP00000289703; ENSG00000158711 [P28324-2]
ENST00000357992; ENSP00000350681; ENSG00000158711 [P28324-1]
ENST00000616704; ENSP00000483628; ENSG00000158711 [P28324-1]
GeneIDi2005
KEGGihsa:2005
UCSCiuc001hcy.3 human [P28324-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiELK4_HUMAN
AccessioniPrimary (citable) accession number: P28324
Secondary accession number(s): P28323, Q6GSJ2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: January 24, 2001
Last modified: May 23, 2018
This is version 172 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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