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Protein

ETS domain-containing protein Elk-4

Gene

ELK4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in both transcriptional activation and repression. Interaction with SIRT7 leads to recruitment and stabilization of SIRT7 at promoters, followed by deacetylation of histone H3 at 'Lys-18' (H3K18Ac) and subsequent transcription repression. Forms a ternary complex with the serum response factor (SRF). Requires DNA-bound SRF for ternary complex formation and makes extensive DNA contacts to the 5'side of SRF, but does not bind DNA autonomously.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi5 – 85ETSPROSITE-ProRule annotationAdd BLAST81

GO - Molecular functioni

  • chromatin binding Source: UniProtKB
  • core promoter binding Source: UniProtKB
  • DNA binding Source: ProtInc
  • RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: NTNU_SB
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: GO_Central
  • transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding Source: NTNU_SB
  • transcription cofactor activity Source: ProtInc
  • transcription factor activity, sequence-specific DNA binding Source: ProtInc

GO - Biological processi

  • cell differentiation Source: GO_Central
  • histone H3 deacetylation Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: NTNU_SB

Keywordsi

Molecular functionActivator, DNA-binding, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

SignaLinkiP28324.
SIGNORiP28324.

Names & Taxonomyi

Protein namesi
Recommended name:
ETS domain-containing protein Elk-4
Alternative name(s):
Serum response factor accessory protein 1
Short name:
SAP-1
Short name:
SRF accessory protein 1
Gene namesi
Name:ELK4
Synonyms:SAP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:3326. ELK4.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi2005.
OpenTargetsiENSG00000158711.
PharmGKBiPA27753.

Polymorphism and mutation databases

BioMutaiELK4.
DMDMi12585557.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002040991 – 431ETS domain-containing protein Elk-4Add BLAST431

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki167Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei180PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP28324.
PaxDbiP28324.
PeptideAtlasiP28324.
PRIDEiP28324.

PTM databases

iPTMnetiP28324.
PhosphoSitePlusiP28324.

Expressioni

Gene expression databases

BgeeiENSG00000158711.
CleanExiHS_ELK4.
ExpressionAtlasiP28324. baseline and differential.
GenevisibleiP28324. HS.

Organism-specific databases

HPAiHPA028863.

Interactioni

Subunit structurei

Interacts with SIRT7.1 Publication

Protein-protein interaction databases

BioGridi108320. 9 interactors.
DIPiDIP-59907N.
IntActiP28324. 1 interactor.
STRINGi9606.ENSP00000350681.

Structurei

Secondary structure

1431
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 14Combined sources8
Helixi18 – 20Combined sources3
Turni21 – 23Combined sources3
Beta strandi24 – 26Combined sources3
Beta strandi28 – 34Combined sources7
Helixi38 – 49Combined sources12
Helixi56 – 69Combined sources14
Beta strandi71 – 74Combined sources4
Beta strandi76 – 78Combined sources3
Beta strandi81 – 84Combined sources4
Turni85 – 88Combined sources4
Helixi89 – 91Combined sources3
Helixi138 – 143Combined sources6
Beta strandi144 – 152Combined sources9
Helixi153 – 155Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BC7X-ray2.01C1-93[»]
1BC8X-ray1.93C1-93[»]
1HBXX-ray3.15G/H2-156[»]
1K6OX-ray3.19A1-93[»]
ProteinModelPortaliP28324.
SMRiP28324.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP28324.

Family & Domainsi

Sequence similaritiesi

Belongs to the ETS family.Curated

Phylogenomic databases

eggNOGiKOG3806. Eukaryota.
ENOG410Z0ZF. LUCA.
GeneTreeiENSGT00760000118996.
HOVERGENiHBG004344.
InParanoidiP28324.
KOiK04376.
OMAiICWTSNN.
OrthoDBiEOG091G0COO.
PhylomeDBiP28324.
TreeFamiTF317732.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiView protein in InterPro
IPR000418. Ets_dom.
IPR011991. WHTH_DNA-bd_dom.
PfamiView protein in Pfam
PF00178. Ets. 1 hit.
PRINTSiPR00454. ETSDOMAIN.
SMARTiView protein in SMART
SM00413. ETS. 1 hit.
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiView protein in PROSITE
PS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P28324-1) [UniParc]FASTAAdd to basket
Also known as: SAP-1A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDSAITLWQF LLQLLQKPQN KHMICWTSND GQFKLLQAEE VARLWGIRKN
60 70 80 90 100
KPNMNYDKLS RALRYYYVKN IIKKVNGQKF VYKFVSYPEI LNMDPMTVGR
110 120 130 140 150
IEGDCESLNF SEVSSSSKDV ENGGKDKPPQ PGAKTSSRND YIHSGLYSSF
160 170 180 190 200
TLNSLNSSNV KLFKLIKTEN PAEKLAEKKS PQEPTPSVIK FVTTPSKKPP
210 220 230 240 250
VEPVAATISI GPSISPSSEE TIQALETLVS PKLPSLEAPT SASNVMTAFA
260 270 280 290 300
TTPPISSIPP LQEPPRTPSP PLSSHPDIDT DIDSVASQPM ELPENLSLEP
310 320 330 340 350
KDQDSVLLEK DKVNNSSRSK KPKGLELAPT LVITSSDPSP LGILSPSLPT
360 370 380 390 400
ASLTPAFFSQ TPIILTPSPL LSSIHFWSTL SPVAPLSPAR LQGANTLFQF
410 420 430
PSVLNSHGPF TLSGLDGPST PGPFSPDLQK T
Length:431
Mass (Da):46,900
Last modified:January 24, 2001 - v3
Checksum:i80EE3E69995C7A7D
GO
Isoform 2 (identifier: P28324-2) [UniParc]FASTAAdd to basket
Also known as: SAP-1B

The sequence of this isoform differs from the canonical sequence as follows:
     361-431: TPIILTPSPL...GPFSPDLQKT → VACSLFMVSP...VLERLCVTVM

Show »
Length:405
Mass (Da):44,674
Checksum:i13DC31EA9C6D5B89
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti326E → G in AAA03631 (PubMed:1339307).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_001468361 – 431TPIIL…DLQKT → VACSLFMVSPLLSFICPFKQ IQNLYTQVCFLLLRFVLERL CVTVM in isoform 2. 1 PublicationAdd BLAST71

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M85165 mRNA. Translation: AAA03631.1.
M85164 mRNA. Translation: AAA03632.1.
CH471067 Genomic DNA. Translation: EAW91570.1.
BC063676 mRNA. Translation: AAH63676.1.
CCDSiCCDS1456.1. [P28324-1]
CCDS1457.1. [P28324-2]
PIRiA42093.
A53012.
B42093.
RefSeqiNP_001964.2. NM_001973.3. [P28324-1]
NP_068567.1. NM_021795.2. [P28324-2]
XP_005245007.1. XM_005244950.4. [P28324-1]
XP_005245008.1. XM_005244951.4. [P28324-1]
UniGeneiHs.497520.
Hs.602654.

Genome annotation databases

EnsembliENST00000289703; ENSP00000289703; ENSG00000158711. [P28324-2]
ENST00000357992; ENSP00000350681; ENSG00000158711. [P28324-1]
ENST00000616704; ENSP00000483628; ENSG00000158711. [P28324-1]
GeneIDi2005.
KEGGihsa:2005.
UCSCiuc001hcy.3. human. [P28324-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiELK4_HUMAN
AccessioniPrimary (citable) accession number: P28324
Secondary accession number(s): P28323, Q6GSJ2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: January 24, 2001
Last modified: August 30, 2017
This is version 166 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families