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P28324

- ELK4_HUMAN

UniProt

P28324 - ELK4_HUMAN

Protein

ETS domain-containing protein Elk-4

Gene

ELK4

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 142 (01 Oct 2014)
      Sequence version 3 (24 Jan 2001)
      Previous versions | rss
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    Functioni

    Involved in both transcriptional activation and repression. Interaction with SIRT7 leads to recruitment and stabilization of SIRT7 at promoters, followed by deacetylation of histone H3 at 'Lys-18' (H3K18Ac) and subsequent transcription repression. Forms a ternary complex with the serum response factor (SRF). Requires DNA-bound SRF for ternary complex formation and makes extensive DNA contacts to the 5'side of SRF, but does not bind DNA autonomously.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi5 – 8581ETSPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. chromatin binding Source: UniProtKB
    2. core promoter binding Source: UniProtKB
    3. DNA binding Source: ProtInc
    4. protein binding Source: UniProtKB
    5. sequence-specific DNA binding Source: InterPro
    6. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: RefGenome
    7. sequence-specific DNA binding transcription factor activity Source: ProtInc
    8. transcription cofactor activity Source: ProtInc

    GO - Biological processi

    1. cell differentiation Source: RefGenome
    2. histone H3 deacetylation Source: UniProtKB
    3. negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
    4. transcription from RNA polymerase II promoter Source: GOC

    Keywords - Molecular functioni

    Activator, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    SignaLinkiP28324.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ETS domain-containing protein Elk-4
    Alternative name(s):
    Serum response factor accessory protein 1
    Short name:
    SAP-1
    Short name:
    SRF accessory protein 1
    Gene namesi
    Name:ELK4
    Synonyms:SAP1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 1

    Organism-specific databases

    HGNCiHGNC:3326. ELK4.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: HPA
    2. nucleus Source: HPA

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA27753.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 431431ETS domain-containing protein Elk-4PRO_0000204099Add
    BLAST

    Proteomic databases

    PaxDbiP28324.
    PRIDEiP28324.

    PTM databases

    PhosphoSiteiP28324.

    Expressioni

    Gene expression databases

    ArrayExpressiP28324.
    BgeeiP28324.
    CleanExiHS_ELK4.
    GenevestigatoriP28324.

    Organism-specific databases

    HPAiHPA028863.

    Interactioni

    Subunit structurei

    Interacts with SIRT7.1 Publication

    Protein-protein interaction databases

    BioGridi108320. 5 interactions.
    DIPiDIP-59907N.
    STRINGi9606.ENSP00000350681.

    Structurei

    Secondary structure

    1
    431
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi7 – 148
    Helixi18 – 203
    Turni21 – 233
    Beta strandi24 – 263
    Beta strandi28 – 347
    Helixi38 – 4912
    Helixi56 – 6914
    Beta strandi71 – 744
    Beta strandi76 – 783
    Beta strandi81 – 844
    Turni85 – 884
    Helixi89 – 913
    Helixi138 – 1436
    Beta strandi144 – 1529
    Helixi153 – 1553

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1BC7X-ray2.01C1-93[»]
    1BC8X-ray1.93C1-93[»]
    1HBXX-ray3.15G/H2-156[»]
    1K6OX-ray3.19A1-93[»]
    ProteinModelPortaliP28324.
    SMRiP28324. Positions 1-156.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP28324.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ETS family.Curated
    Contains 1 ETS DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG317055.
    HOVERGENiHBG004344.
    InParanoidiP28324.
    KOiK04376.
    OMAiICWTSNN.
    OrthoDBiEOG7NPFTD.
    PhylomeDBiP28324.
    TreeFamiTF317732.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    InterProiIPR000418. Ets_dom.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00178. Ets. 1 hit.
    [Graphical view]
    PRINTSiPR00454. ETSDOMAIN.
    SMARTiSM00413. ETS. 1 hit.
    [Graphical view]
    PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
    PS00346. ETS_DOMAIN_2. 1 hit.
    PS50061. ETS_DOMAIN_3. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P28324-1) [UniParc]FASTAAdd to Basket

    Also known as: SAP-1A

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDSAITLWQF LLQLLQKPQN KHMICWTSND GQFKLLQAEE VARLWGIRKN    50
    KPNMNYDKLS RALRYYYVKN IIKKVNGQKF VYKFVSYPEI LNMDPMTVGR 100
    IEGDCESLNF SEVSSSSKDV ENGGKDKPPQ PGAKTSSRND YIHSGLYSSF 150
    TLNSLNSSNV KLFKLIKTEN PAEKLAEKKS PQEPTPSVIK FVTTPSKKPP 200
    VEPVAATISI GPSISPSSEE TIQALETLVS PKLPSLEAPT SASNVMTAFA 250
    TTPPISSIPP LQEPPRTPSP PLSSHPDIDT DIDSVASQPM ELPENLSLEP 300
    KDQDSVLLEK DKVNNSSRSK KPKGLELAPT LVITSSDPSP LGILSPSLPT 350
    ASLTPAFFSQ TPIILTPSPL LSSIHFWSTL SPVAPLSPAR LQGANTLFQF 400
    PSVLNSHGPF TLSGLDGPST PGPFSPDLQK T 431
    Length:431
    Mass (Da):46,900
    Last modified:January 24, 2001 - v3
    Checksum:i80EE3E69995C7A7D
    GO
    Isoform 2 (identifier: P28324-2) [UniParc]FASTAAdd to Basket

    Also known as: SAP-1B

    The sequence of this isoform differs from the canonical sequence as follows:
         361-431: TPIILTPSPL...GPFSPDLQKT → VACSLFMVSP...VLERLCVTVM

    Show »
    Length:405
    Mass (Da):44,674
    Checksum:i13DC31EA9C6D5B89
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti326 – 3261E → G in AAA03631. (PubMed:1339307)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei361 – 43171TPIIL…DLQKT → VACSLFMVSPLLSFICPFKQ IQNLYTQVCFLLLRFVLERL CVTVM in isoform 2. 1 PublicationVSP_001468Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M85165 mRNA. Translation: AAA03631.1.
    M85164 mRNA. Translation: AAA03632.1.
    CH471067 Genomic DNA. Translation: EAW91570.1.
    BC063676 mRNA. Translation: AAH63676.1.
    CCDSiCCDS1456.1. [P28324-1]
    CCDS1457.1. [P28324-2]
    PIRiA42093.
    A53012.
    B42093.
    RefSeqiNP_001964.2. NM_001973.3. [P28324-1]
    NP_068567.1. NM_021795.2. [P28324-2]
    XP_005245007.1. XM_005244950.2. [P28324-1]
    XP_005245008.1. XM_005244951.2. [P28324-1]
    UniGeneiHs.497520.
    Hs.602654.

    Genome annotation databases

    EnsembliENST00000289703; ENSP00000289703; ENSG00000158711. [P28324-2]
    ENST00000357992; ENSP00000350681; ENSG00000158711. [P28324-1]
    GeneIDi2005.
    KEGGihsa:2005.
    UCSCiuc001hcy.2. human. [P28324-1]
    uc001hcz.3. human. [P28324-2]

    Polymorphism databases

    DMDMi12585557.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M85165 mRNA. Translation: AAA03631.1 .
    M85164 mRNA. Translation: AAA03632.1 .
    CH471067 Genomic DNA. Translation: EAW91570.1 .
    BC063676 mRNA. Translation: AAH63676.1 .
    CCDSi CCDS1456.1. [P28324-1 ]
    CCDS1457.1. [P28324-2 ]
    PIRi A42093.
    A53012.
    B42093.
    RefSeqi NP_001964.2. NM_001973.3. [P28324-1 ]
    NP_068567.1. NM_021795.2. [P28324-2 ]
    XP_005245007.1. XM_005244950.2. [P28324-1 ]
    XP_005245008.1. XM_005244951.2. [P28324-1 ]
    UniGenei Hs.497520.
    Hs.602654.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1BC7 X-ray 2.01 C 1-93 [» ]
    1BC8 X-ray 1.93 C 1-93 [» ]
    1HBX X-ray 3.15 G/H 2-156 [» ]
    1K6O X-ray 3.19 A 1-93 [» ]
    ProteinModelPortali P28324.
    SMRi P28324. Positions 1-156.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 108320. 5 interactions.
    DIPi DIP-59907N.
    STRINGi 9606.ENSP00000350681.

    PTM databases

    PhosphoSitei P28324.

    Polymorphism databases

    DMDMi 12585557.

    Proteomic databases

    PaxDbi P28324.
    PRIDEi P28324.

    Protocols and materials databases

    DNASUi 2005.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000289703 ; ENSP00000289703 ; ENSG00000158711 . [P28324-2 ]
    ENST00000357992 ; ENSP00000350681 ; ENSG00000158711 . [P28324-1 ]
    GeneIDi 2005.
    KEGGi hsa:2005.
    UCSCi uc001hcy.2. human. [P28324-1 ]
    uc001hcz.3. human. [P28324-2 ]

    Organism-specific databases

    CTDi 2005.
    GeneCardsi GC01M205577.
    HGNCi HGNC:3326. ELK4.
    HPAi HPA028863.
    MIMi 600246. gene.
    neXtProti NX_P28324.
    PharmGKBi PA27753.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG317055.
    HOVERGENi HBG004344.
    InParanoidi P28324.
    KOi K04376.
    OMAi ICWTSNN.
    OrthoDBi EOG7NPFTD.
    PhylomeDBi P28324.
    TreeFami TF317732.

    Enzyme and pathway databases

    SignaLinki P28324.

    Miscellaneous databases

    EvolutionaryTracei P28324.
    GeneWikii ELK4.
    GenomeRNAii 2005.
    NextBioi 8113.
    PROi P28324.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P28324.
    Bgeei P28324.
    CleanExi HS_ELK4.
    Genevestigatori P28324.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    InterProi IPR000418. Ets_dom.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00178. Ets. 1 hit.
    [Graphical view ]
    PRINTSi PR00454. ETSDOMAIN.
    SMARTi SM00413. ETS. 1 hit.
    [Graphical view ]
    PROSITEi PS00345. ETS_DOMAIN_1. 1 hit.
    PS00346. ETS_DOMAIN_2. 1 hit.
    PS50061. ETS_DOMAIN_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of SAP-1, a protein recruited by serum response factor to the c-fos serum response element."
      Dalton S., Treisman R.
      Cell 68:597-612(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
    2. "Characterization of SAP-1, a protein recruited by serum response factor to the c-fos serum response element."
      Dalton S., Treisman R.
      Cell 76:411-411(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: SEQUENCE REVISION.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Lymph.
    5. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.
    6. "Structures of SAP-1 bound to DNA targets from the E74 and c-fos promoters: insights into DNA sequence discrimination by Ets proteins."
      Mo Y., Vaessen B., Johnston K., Marmorstein R.
      Mol. Cell 2:201-212(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.01 ANGSTROMS) OF 1-93.
    7. Cited for: FUNCTION, INTERACTION WITH SIRT7.

    Entry informationi

    Entry nameiELK4_HUMAN
    AccessioniPrimary (citable) accession number: P28324
    Secondary accession number(s): P28323, Q6GSJ2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: January 24, 2001
    Last modified: October 1, 2014
    This is version 142 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3