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P28322 (ETV4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ETS translocation variant 4
Alternative name(s):
Polyomavirus enhancer activator 3
Short name=Protein PEA3
Gene names
Name:Etv4
Synonyms:Pea-3, Pea3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length485 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transcriptional activator that binds to the enhancer of the adenovirus E1A gene; the core-binding sequence is 5'[AC]GGA[AT]GT-3'. May play a regulatory role during embryogenesis.

Subcellular location

Nucleus.

Tissue specificity

Epididymis and brain.

Post-translational modification

Phosphorylated.

Sumoylated; enhanced upon ERK/MAP kinase pathway activation it positively regulates the transcriptional activator capacity. Sumoylation at Lys-95 probably requires phosphorylation at Ser-100. Transiently polysumoylated and desumoylated by SENP1. Sumoylation is a prerequisite to polyubiquitination which in turn increases proteasomal-mediated degradation. Probably polyubiquitinated by RNF4 and deubiquitinated by USP2 By similarity.

Sequence similarities

Belongs to the ETS family.

Contains 1 ETS DNA-binding domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   LigandDNA-binding
   Molecular functionActivator
   PTMIsopeptide bond
Phosphoprotein
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processbranching involved in mammary gland duct morphogenesis

Inferred from mutant phenotype PubMed 18977342. Source: MGI

motor neuron axon guidance

Inferred from mutant phenotype PubMed 12372283. Source: MGI

negative regulation of mammary gland epithelial cell proliferation

Inferred from mutant phenotype PubMed 18977342. Source: MGI

positive regulation of gene expression

Inferred from direct assay PubMed 15253939. Source: UniProtKB

positive regulation of transcription, DNA-templated

Inferred from direct assay PubMed 15466854. Source: MGI

regulation of branching involved in mammary gland duct morphogenesis

Inferred from direct assay PubMed 12871699. Source: MGI

regulation of transcription from RNA polymerase II promoter

Inferred from Biological aspect of Ancestor. Source: RefGenome

stem cell differentiation

Inferred from mutant phenotype PubMed 18977342. Source: MGI

transcription from RNA polymerase II promoter

Inferred from Biological aspect of Ancestor. Source: GOC

   Cellular_componentintracellular

Inferred from direct assay PubMed 10482234. Source: MGI

nucleolus

Inferred from electronic annotation. Source: Ensembl

nucleus

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Molecular_functionDNA binding

Inferred from direct assay PubMed 12527199. Source: MGI

sequence-specific DNA binding

Inferred from electronic annotation. Source: InterPro

sequence-specific DNA binding RNA polymerase II transcription factor activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P28322-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P28322-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MTKSSNHNCL...GPVAGGSGRM
     86-86: S → LA
     127-132: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 485485ETS translocation variant 4
PRO_0000204117

Regions

DNA binding342 – 42281ETS By similarity
Compositional bias49 – 7527Asp/Glu-rich (acidic)
Compositional bias147 – 24599Gln-rich

Amino acid modifications

Modified residue1001Phosphoserine By similarity
Cross-link95Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
Cross-link227Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
Cross-link261Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity

Natural variations

Alternative sequence11M → MTKSSNHNCLLRPENKPGLW GPGAQAASLRPSPATLVVSS PGHAEHPPAAPAQTPGPQVS ASARGPGPVAGGSGRM in isoform 2.
VSP_047943
Alternative sequence861S → LA in isoform 2.
VSP_047944
Alternative sequence127 – 1326Missing in isoform 2.
VSP_047945

Experimental info

Sequence conflict3161E → K in CAA44872. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified September 18, 2013. Version 2.
Checksum: 3A5FF4EFD7E828EF

FASTA48554,008
        10         20         30         40         50         60 
MERRMKGGYL DQRVPYTFCS KSPGNGSLGE ALMVPQGKLM DPGSLPPSDS EDLFQDLSHF 

        70         80         90        100        110        120 
QETWLAEAQV PDSDEQFVPD FHSENSFHSP TTRIKKEPQS PRTDPALSCS RKPPLPYHHG 

       130        140        150        160        170        180 
EQCLYSSAYD SPRQIAIKSP APGAPGQSPL QPFSRAEQQQ SLLRASSSSQ SHPGHGYLGE 

       190        200        210        220        230        240 
HSSVFQQPVD MCHSFTSPQG GGREPLPAPY QHQLSEPCPP YPQQNFKQEY HDPLYEQAGQ 

       250        260        270        280        290        300 
PASSQGGVSG HRYPGAGVVI KQERTDFAYD SDVPGCASMY LHPEGFSGPS PGDGVMGYGY 

       310        320        330        340        350        360 
EKSLRPFPDD VCIVPEKFEG DIKQEGIGAF REGPPYQRRG ALQLWQFLVA LLDDPTNAHF 

       370        380        390        400        410        420 
IAWTGRGMEF KLIEPEEVAR LWGIQKNRPA MNYDKLSRSL RYYYEKGIMQ KVAGERYVYK 

       430        440        450        460        470        480 
FVCEPEALFS LAFPDNQRPA LKAEFDRPVS EEDTVPLSHL DESPAYLPEL TGPAPPFGHR 


GGYSY 

« Hide

Isoform 2 [UniParc].

Checksum: 778AB1AF19B202BF
Show »

FASTA55560,847

References

« Hide 'large scale' references
[1]"Molecular cloning and characterization of PEA3, a new member of the Ets oncogene family that is differentially expressed in mouse embryonic cells."
Xin J.-H., Cowie A., Lachance P., Hassell J.A.
Genes Dev. 6:481-496(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Lung.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X63190 mRNA. Translation: CAA44872.1.
AK144590 mRNA. Translation: BAE25952.1.
AL591436 Genomic DNA. No translation available.
CCDSCCDS48937.1. [P28322-1]
PIRS24061.
RefSeqNP_032841.2. NM_008815.2. [P28322-1]
UniGeneMm.5025.

3D structure databases

ProteinModelPortalP28322.
SMRP28322. Positions 339-435.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-60466N.

PTM databases

PhosphoSiteP28322.

Proteomic databases

PRIDEP28322.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000164750; ENSMUSP00000129261; ENSMUSG00000017724. [P28322-1]
GeneID18612.
KEGGmmu:18612.
UCSCuc007lpt.1. mouse.

Organism-specific databases

CTD2118.
MGIMGI:99423. Etv4.

Phylogenomic databases

eggNOGNOG258471.
GeneTreeENSGT00750000117517.
HOGENOMHOG000230986.
HOVERGENHBG000231.
KOK15592.

Gene expression databases

GenevestigatorP28322.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
InterProIPR000418. Ets_dom.
IPR006715. ETS_PEA3_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF00178. Ets. 1 hit.
PF04621. ETS_PEA3_N. 1 hit.
[Graphical view]
PRINTSPR00454. ETSDOMAIN.
SMARTSM00413. ETS. 1 hit.
[Graphical view]
PROSITEPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSETV4. mouse.
NextBio294546.
PROP28322.
SOURCESearch...

Entry information

Entry nameETV4_MOUSE
AccessionPrimary (citable) accession number: P28322
Secondary accession number(s): Q3UMZ6
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: September 18, 2013
Last modified: July 9, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot