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P28322

- ETV4_MOUSE

UniProt

P28322 - ETV4_MOUSE

Protein

ETS translocation variant 4

Gene

Etv4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 2 (18 Sep 2013)
      Previous versions | rss
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    Functioni

    Transcriptional activator that binds to the enhancer of the adenovirus E1A gene; the core-binding sequence is 5'[AC]GGA[AT]GT-3'. May play a regulatory role during embryogenesis.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi342 – 42281ETSPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: MGI
    2. sequence-specific DNA binding Source: InterPro
    3. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: RefGenome

    GO - Biological processi

    1. branching involved in mammary gland duct morphogenesis Source: MGI
    2. motor neuron axon guidance Source: MGI
    3. negative regulation of mammary gland epithelial cell proliferation Source: MGI
    4. positive regulation of gene expression Source: UniProtKB
    5. positive regulation of transcription, DNA-templated Source: MGI
    6. positive regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
    7. regulation of branching involved in mammary gland duct morphogenesis Source: MGI
    8. regulation of transcription from RNA polymerase II promoter Source: RefGenome
    9. stem cell differentiation Source: MGI
    10. transcription from RNA polymerase II promoter Source: GOC

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ETS translocation variant 4
    Alternative name(s):
    Polyomavirus enhancer activator 3
    Short name:
    Protein PEA3
    Gene namesi
    Name:Etv4
    Synonyms:Pea-3, Pea3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:99423. Etv4.

    Subcellular locationi

    GO - Cellular componenti

    1. intracellular Source: MGI
    2. nucleolus Source: Ensembl
    3. nucleus Source: RefGenome

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 485485ETS translocation variant 4PRO_0000204117Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki95 – 95Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
    Modified residuei100 – 1001PhosphoserineBy similarity
    Cross-linki227 – 227Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
    Cross-linki261 – 261Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity

    Post-translational modificationi

    Phosphorylated.
    Sumoylated; enhanced upon ERK/MAP kinase pathway activation it positively regulates the transcriptional activator capacity. Sumoylation at Lys-95 probably requires phosphorylation at Ser-100. Transiently polysumoylated and desumoylated by SENP1. Sumoylation is a prerequisite to polyubiquitination which in turn increases proteasomal-mediated degradation. Probably polyubiquitinated by RNF4 and deubiquitinated by USP2 By similarity.By similarity

    Keywords - PTMi

    Isopeptide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PRIDEiP28322.

    PTM databases

    PhosphoSiteiP28322.

    Expressioni

    Tissue specificityi

    Epididymis and brain.

    Gene expression databases

    GenevestigatoriP28322.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-60466N.

    Structurei

    3D structure databases

    ProteinModelPortaliP28322.
    SMRiP28322. Positions 339-435.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi49 – 7527Asp/Glu-rich (acidic)Add
    BLAST
    Compositional biasi147 – 24599Gln-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ETS family.Curated
    Contains 1 ETS DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG258471.
    GeneTreeiENSGT00750000117517.
    HOGENOMiHOG000230986.
    HOVERGENiHBG000231.
    KOiK15592.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    InterProiIPR000418. Ets_dom.
    IPR006715. ETS_PEA3_N.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00178. Ets. 1 hit.
    PF04621. ETS_PEA3_N. 1 hit.
    [Graphical view]
    PRINTSiPR00454. ETSDOMAIN.
    SMARTiSM00413. ETS. 1 hit.
    [Graphical view]
    PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
    PS00346. ETS_DOMAIN_2. 1 hit.
    PS50061. ETS_DOMAIN_3. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P28322-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MERRMKGGYL DQRVPYTFCS KSPGNGSLGE ALMVPQGKLM DPGSLPPSDS    50
    EDLFQDLSHF QETWLAEAQV PDSDEQFVPD FHSENSFHSP TTRIKKEPQS 100
    PRTDPALSCS RKPPLPYHHG EQCLYSSAYD SPRQIAIKSP APGAPGQSPL 150
    QPFSRAEQQQ SLLRASSSSQ SHPGHGYLGE HSSVFQQPVD MCHSFTSPQG 200
    GGREPLPAPY QHQLSEPCPP YPQQNFKQEY HDPLYEQAGQ PASSQGGVSG 250
    HRYPGAGVVI KQERTDFAYD SDVPGCASMY LHPEGFSGPS PGDGVMGYGY 300
    EKSLRPFPDD VCIVPEKFEG DIKQEGIGAF REGPPYQRRG ALQLWQFLVA 350
    LLDDPTNAHF IAWTGRGMEF KLIEPEEVAR LWGIQKNRPA MNYDKLSRSL 400
    RYYYEKGIMQ KVAGERYVYK FVCEPEALFS LAFPDNQRPA LKAEFDRPVS 450
    EEDTVPLSHL DESPAYLPEL TGPAPPFGHR GGYSY 485
    Length:485
    Mass (Da):54,008
    Last modified:September 18, 2013 - v2
    Checksum:i3A5FF4EFD7E828EF
    GO
    Isoform 2 (identifier: P28322-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-1: M → MTKSSNHNCL...GPVAGGSGRM
         86-86: S → LA
         127-132: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:555
    Mass (Da):60,847
    Checksum:i778AB1AF19B202BF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti316 – 3161E → K in CAA44872. (PubMed:1547944)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 11M → MTKSSNHNCLLRPENKPGLW GPGAQAASLRPSPATLVVSS PGHAEHPPAAPAQTPGPQVS ASARGPGPVAGGSGRM in isoform 2. 1 PublicationVSP_047943
    Alternative sequencei86 – 861S → LA in isoform 2. 1 PublicationVSP_047944
    Alternative sequencei127 – 1326Missing in isoform 2. 1 PublicationVSP_047945

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X63190 mRNA. Translation: CAA44872.1.
    AK144590 mRNA. Translation: BAE25952.1.
    AL591436 Genomic DNA. No translation available.
    CCDSiCCDS48937.1. [P28322-1]
    PIRiS24061.
    RefSeqiNP_032841.2. NM_008815.2. [P28322-1]
    UniGeneiMm.5025.

    Genome annotation databases

    EnsembliENSMUST00000164750; ENSMUSP00000129261; ENSMUSG00000017724. [P28322-1]
    GeneIDi18612.
    KEGGimmu:18612.
    UCSCiuc007lpt.1. mouse.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X63190 mRNA. Translation: CAA44872.1 .
    AK144590 mRNA. Translation: BAE25952.1 .
    AL591436 Genomic DNA. No translation available.
    CCDSi CCDS48937.1. [P28322-1 ]
    PIRi S24061.
    RefSeqi NP_032841.2. NM_008815.2. [P28322-1 ]
    UniGenei Mm.5025.

    3D structure databases

    ProteinModelPortali P28322.
    SMRi P28322. Positions 339-435.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-60466N.

    PTM databases

    PhosphoSitei P28322.

    Proteomic databases

    PRIDEi P28322.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000164750 ; ENSMUSP00000129261 ; ENSMUSG00000017724 . [P28322-1 ]
    GeneIDi 18612.
    KEGGi mmu:18612.
    UCSCi uc007lpt.1. mouse.

    Organism-specific databases

    CTDi 2118.
    MGIi MGI:99423. Etv4.

    Phylogenomic databases

    eggNOGi NOG258471.
    GeneTreei ENSGT00750000117517.
    HOGENOMi HOG000230986.
    HOVERGENi HBG000231.
    KOi K15592.

    Miscellaneous databases

    ChiTaRSi ETV4. mouse.
    NextBioi 294546.
    PROi P28322.
    SOURCEi Search...

    Gene expression databases

    Genevestigatori P28322.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    InterProi IPR000418. Ets_dom.
    IPR006715. ETS_PEA3_N.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00178. Ets. 1 hit.
    PF04621. ETS_PEA3_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00454. ETSDOMAIN.
    SMARTi SM00413. ETS. 1 hit.
    [Graphical view ]
    PROSITEi PS00345. ETS_DOMAIN_1. 1 hit.
    PS00346. ETS_DOMAIN_2. 1 hit.
    PS50061. ETS_DOMAIN_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of PEA3, a new member of the Ets oncogene family that is differentially expressed in mouse embryonic cells."
      Xin J.-H., Cowie A., Lachance P., Hassell J.A.
      Genes Dev. 6:481-496(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Lung.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.

    Entry informationi

    Entry nameiETV4_MOUSE
    AccessioniPrimary (citable) accession number: P28322
    Secondary accession number(s): Q3UMZ6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: September 18, 2013
    Last modified: October 1, 2014
    This is version 117 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3