Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ETS translocation variant 4

Gene

Etv4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Transcriptional activator that binds to the enhancer of the adenovirus E1A gene; the core-binding sequence is 5'[AC]GGA[AT]GT-3'. May play a regulatory role during embryogenesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi342 – 422ETSPROSITE-ProRule annotationAdd BLAST81

GO - Molecular functioni

GO - Biological processi

  • branching involved in mammary gland duct morphogenesis Source: MGI
  • motor neuron axon guidance Source: MGI
  • negative regulation of mammary gland epithelial cell proliferation Source: MGI
  • positive regulation of gene expression Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • regulation of branching involved in mammary gland duct morphogenesis Source: MGI
  • stem cell differentiation Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-5687128. MAPK6/MAPK4 signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
ETS translocation variant 4
Alternative name(s):
Polyomavirus enhancer activator 3
Short name:
Protein PEA3
Gene namesi
Name:Etv4
Synonyms:Pea-3, Pea3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:99423. Etv4.

Subcellular locationi

GO - Cellular componenti

  • intracellular Source: MGI
  • nucleolus Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002041171 – 485ETS translocation variant 4Add BLAST485

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki95Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei100PhosphoserineBy similarity1
Modified residuei139PhosphoserineBy similarity1
Modified residuei148PhosphoserineBy similarity1
Modified residuei215PhosphoserineBy similarity1
Cross-linki227Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Cross-linki261Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Cross-linki323Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Post-translational modificationi

Phosphorylated.
Sumoylated; enhanced upon ERK/MAP kinase pathway activation it positively regulates the transcriptional activator capacity. Sumoylation at Lys-95 probably requires phosphorylation at Ser-100. Transiently polysumoylated and desumoylated by SENP1. Sumoylation is a prerequisite to polyubiquitination which in turn increases proteasomal-mediated degradation. Probably polyubiquitinated by RNF4 and deubiquitinated by USP2 (By similarity).By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP28322.
PRIDEiP28322.

PTM databases

iPTMnetiP28322.
PhosphoSitePlusiP28322.

Expressioni

Tissue specificityi

Epididymis and brain.

Gene expression databases

BgeeiENSMUSG00000017724.
ExpressionAtlasiP28322. baseline and differential.
GenevisibleiP28322. MM.

Interactioni

Protein-protein interaction databases

BioGridi202104. 2 interactors.
DIPiDIP-60466N.
STRINGi10090.ENSMUSP00000129261.

Structurei

3D structure databases

ProteinModelPortaliP28322.
SMRiP28322.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi49 – 75Asp/Glu-rich (acidic)Add BLAST27
Compositional biasi147 – 245Gln-richAdd BLAST99

Sequence similaritiesi

Belongs to the ETS family.Curated
Contains 1 ETS DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3806. Eukaryota.
ENOG410Z0ZF. LUCA.
GeneTreeiENSGT00760000118907.
HOGENOMiHOG000230986.
HOVERGENiHBG000231.
InParanoidiP28322.
KOiK15592.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000418. Ets_dom.
IPR006715. ETS_PEA3_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00178. Ets. 1 hit.
PF04621. ETS_PEA3_N. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P28322-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MERRMKGGYL DQRVPYTFCS KSPGNGSLGE ALMVPQGKLM DPGSLPPSDS
60 70 80 90 100
EDLFQDLSHF QETWLAEAQV PDSDEQFVPD FHSENSFHSP TTRIKKEPQS
110 120 130 140 150
PRTDPALSCS RKPPLPYHHG EQCLYSSAYD SPRQIAIKSP APGAPGQSPL
160 170 180 190 200
QPFSRAEQQQ SLLRASSSSQ SHPGHGYLGE HSSVFQQPVD MCHSFTSPQG
210 220 230 240 250
GGREPLPAPY QHQLSEPCPP YPQQNFKQEY HDPLYEQAGQ PASSQGGVSG
260 270 280 290 300
HRYPGAGVVI KQERTDFAYD SDVPGCASMY LHPEGFSGPS PGDGVMGYGY
310 320 330 340 350
EKSLRPFPDD VCIVPEKFEG DIKQEGIGAF REGPPYQRRG ALQLWQFLVA
360 370 380 390 400
LLDDPTNAHF IAWTGRGMEF KLIEPEEVAR LWGIQKNRPA MNYDKLSRSL
410 420 430 440 450
RYYYEKGIMQ KVAGERYVYK FVCEPEALFS LAFPDNQRPA LKAEFDRPVS
460 470 480
EEDTVPLSHL DESPAYLPEL TGPAPPFGHR GGYSY
Length:485
Mass (Da):54,008
Last modified:September 18, 2013 - v2
Checksum:i3A5FF4EFD7E828EF
GO
Isoform 2 (identifier: P28322-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MTKSSNHNCL...GPVAGGSGRM
     86-86: S → LA
     127-132: Missing.

Note: No experimental confirmation available.
Show »
Length:555
Mass (Da):60,847
Checksum:i778AB1AF19B202BF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti316E → K in CAA44872 (PubMed:1547944).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0479431M → MTKSSNHNCLLRPENKPGLW GPGAQAASLRPSPATLVVSS PGHAEHPPAAPAQTPGPQVS ASARGPGPVAGGSGRM in isoform 2. 1 Publication1
Alternative sequenceiVSP_04794486S → LA in isoform 2. 1 Publication1
Alternative sequenceiVSP_047945127 – 132Missing in isoform 2. 1 Publication6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63190 mRNA. Translation: CAA44872.1.
AK144590 mRNA. Translation: BAE25952.1.
AL591436 Genomic DNA. No translation available.
CCDSiCCDS48937.1. [P28322-1]
PIRiS24061.
RefSeqiNP_032841.2. NM_008815.3. [P28322-1]
UniGeneiMm.5025.

Genome annotation databases

EnsembliENSMUST00000164750; ENSMUSP00000129261; ENSMUSG00000017724. [P28322-1]
GeneIDi18612.
KEGGimmu:18612.
UCSCiuc007lpt.1. mouse. [P28322-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63190 mRNA. Translation: CAA44872.1.
AK144590 mRNA. Translation: BAE25952.1.
AL591436 Genomic DNA. No translation available.
CCDSiCCDS48937.1. [P28322-1]
PIRiS24061.
RefSeqiNP_032841.2. NM_008815.3. [P28322-1]
UniGeneiMm.5025.

3D structure databases

ProteinModelPortaliP28322.
SMRiP28322.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202104. 2 interactors.
DIPiDIP-60466N.
STRINGi10090.ENSMUSP00000129261.

PTM databases

iPTMnetiP28322.
PhosphoSitePlusiP28322.

Proteomic databases

PaxDbiP28322.
PRIDEiP28322.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000164750; ENSMUSP00000129261; ENSMUSG00000017724. [P28322-1]
GeneIDi18612.
KEGGimmu:18612.
UCSCiuc007lpt.1. mouse. [P28322-1]

Organism-specific databases

CTDi2118.
MGIiMGI:99423. Etv4.

Phylogenomic databases

eggNOGiKOG3806. Eukaryota.
ENOG410Z0ZF. LUCA.
GeneTreeiENSGT00760000118907.
HOGENOMiHOG000230986.
HOVERGENiHBG000231.
InParanoidiP28322.
KOiK15592.

Enzyme and pathway databases

ReactomeiR-MMU-5687128. MAPK6/MAPK4 signaling.

Miscellaneous databases

ChiTaRSiEtv4. mouse.
PROiP28322.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000017724.
ExpressionAtlasiP28322. baseline and differential.
GenevisibleiP28322. MM.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000418. Ets_dom.
IPR006715. ETS_PEA3_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00178. Ets. 1 hit.
PF04621. ETS_PEA3_N. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiETV4_MOUSE
AccessioniPrimary (citable) accession number: P28322
Secondary accession number(s): Q3UMZ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: September 18, 2013
Last modified: November 2, 2016
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.