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P28322

- ETV4_MOUSE

UniProt

P28322 - ETV4_MOUSE

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Protein
ETS translocation variant 4
Gene
Etv4, Pea-3, Pea3
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Transcriptional activator that binds to the enhancer of the adenovirus E1A gene; the core-binding sequence is 5'[AC]GGA[AT]GT-3'. May play a regulatory role during embryogenesis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi342 – 42281ETS By similarity
Add
BLAST

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. sequence-specific DNA binding Source: InterPro
  3. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: RefGenome
Complete GO annotation...

GO - Biological processi

  1. branching involved in mammary gland duct morphogenesis Source: MGI
  2. motor neuron axon guidance Source: MGI
  3. negative regulation of mammary gland epithelial cell proliferation Source: MGI
  4. positive regulation of gene expression Source: UniProtKB
  5. positive regulation of transcription, DNA-templated Source: MGI
  6. regulation of branching involved in mammary gland duct morphogenesis Source: MGI
  7. regulation of transcription from RNA polymerase II promoter Source: RefGenome
  8. stem cell differentiation Source: MGI
  9. transcription from RNA polymerase II promoter Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ETS translocation variant 4
Alternative name(s):
Polyomavirus enhancer activator 3
Short name:
Protein PEA3
Gene namesi
Name:Etv4
Synonyms:Pea-3, Pea3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:99423. Etv4.

Subcellular locationi

GO - Cellular componenti

  1. intracellular Source: MGI
  2. nucleolus Source: Ensembl
  3. nucleus Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 485485ETS translocation variant 4
PRO_0000204117Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki95 – 95Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
Modified residuei100 – 1001Phosphoserine By similarity
Cross-linki227 – 227Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
Cross-linki261 – 261Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity

Post-translational modificationi

Phosphorylated.
Sumoylated; enhanced upon ERK/MAP kinase pathway activation it positively regulates the transcriptional activator capacity. Sumoylation at Lys-95 probably requires phosphorylation at Ser-100. Transiently polysumoylated and desumoylated by SENP1. Sumoylation is a prerequisite to polyubiquitination which in turn increases proteasomal-mediated degradation. Probably polyubiquitinated by RNF4 and deubiquitinated by USP2 By similarity.

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiP28322.

PTM databases

PhosphoSiteiP28322.

Expressioni

Tissue specificityi

Epididymis and brain.

Gene expression databases

GenevestigatoriP28322.

Interactioni

Protein-protein interaction databases

DIPiDIP-60466N.

Structurei

3D structure databases

ProteinModelPortaliP28322.
SMRiP28322. Positions 339-435.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi49 – 7527Asp/Glu-rich (acidic)
Add
BLAST
Compositional biasi147 – 24599Gln-rich
Add
BLAST

Sequence similaritiesi

Belongs to the ETS family.

Phylogenomic databases

eggNOGiNOG258471.
GeneTreeiENSGT00750000117517.
HOGENOMiHOG000230986.
HOVERGENiHBG000231.
KOiK15592.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000418. Ets_dom.
IPR006715. ETS_PEA3_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00178. Ets. 1 hit.
PF04621. ETS_PEA3_N. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
[Graphical view]
PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P28322-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MERRMKGGYL DQRVPYTFCS KSPGNGSLGE ALMVPQGKLM DPGSLPPSDS    50
EDLFQDLSHF QETWLAEAQV PDSDEQFVPD FHSENSFHSP TTRIKKEPQS 100
PRTDPALSCS RKPPLPYHHG EQCLYSSAYD SPRQIAIKSP APGAPGQSPL 150
QPFSRAEQQQ SLLRASSSSQ SHPGHGYLGE HSSVFQQPVD MCHSFTSPQG 200
GGREPLPAPY QHQLSEPCPP YPQQNFKQEY HDPLYEQAGQ PASSQGGVSG 250
HRYPGAGVVI KQERTDFAYD SDVPGCASMY LHPEGFSGPS PGDGVMGYGY 300
EKSLRPFPDD VCIVPEKFEG DIKQEGIGAF REGPPYQRRG ALQLWQFLVA 350
LLDDPTNAHF IAWTGRGMEF KLIEPEEVAR LWGIQKNRPA MNYDKLSRSL 400
RYYYEKGIMQ KVAGERYVYK FVCEPEALFS LAFPDNQRPA LKAEFDRPVS 450
EEDTVPLSHL DESPAYLPEL TGPAPPFGHR GGYSY 485
Length:485
Mass (Da):54,008
Last modified:September 18, 2013 - v2
Checksum:i3A5FF4EFD7E828EF
GO
Isoform 2 (identifier: P28322-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MTKSSNHNCL...GPVAGGSGRM
     86-86: S → LA
     127-132: Missing.

Note: No experimental confirmation available.

Show »
Length:555
Mass (Da):60,847
Checksum:i778AB1AF19B202BF
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 11M → MTKSSNHNCLLRPENKPGLW GPGAQAASLRPSPATLVVSS PGHAEHPPAAPAQTPGPQVS ASARGPGPVAGGSGRM in isoform 2.
VSP_047943
Alternative sequencei86 – 861S → LA in isoform 2.
VSP_047944
Alternative sequencei127 – 1326Missing in isoform 2.
VSP_047945

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti316 – 3161E → K in CAA44872. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X63190 mRNA. Translation: CAA44872.1.
AK144590 mRNA. Translation: BAE25952.1.
AL591436 Genomic DNA. No translation available.
CCDSiCCDS48937.1. [P28322-1]
PIRiS24061.
RefSeqiNP_032841.2. NM_008815.2. [P28322-1]
UniGeneiMm.5025.

Genome annotation databases

EnsembliENSMUST00000164750; ENSMUSP00000129261; ENSMUSG00000017724. [P28322-1]
GeneIDi18612.
KEGGimmu:18612.
UCSCiuc007lpt.1. mouse.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X63190 mRNA. Translation: CAA44872.1 .
AK144590 mRNA. Translation: BAE25952.1 .
AL591436 Genomic DNA. No translation available.
CCDSi CCDS48937.1. [P28322-1 ]
PIRi S24061.
RefSeqi NP_032841.2. NM_008815.2. [P28322-1 ]
UniGenei Mm.5025.

3D structure databases

ProteinModelPortali P28322.
SMRi P28322. Positions 339-435.
ModBasei Search...

Protein-protein interaction databases

DIPi DIP-60466N.

PTM databases

PhosphoSitei P28322.

Proteomic databases

PRIDEi P28322.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000164750 ; ENSMUSP00000129261 ; ENSMUSG00000017724 . [P28322-1 ]
GeneIDi 18612.
KEGGi mmu:18612.
UCSCi uc007lpt.1. mouse.

Organism-specific databases

CTDi 2118.
MGIi MGI:99423. Etv4.

Phylogenomic databases

eggNOGi NOG258471.
GeneTreei ENSGT00750000117517.
HOGENOMi HOG000230986.
HOVERGENi HBG000231.
KOi K15592.

Miscellaneous databases

ChiTaRSi ETV4. mouse.
NextBioi 294546.
PROi P28322.
SOURCEi Search...

Gene expression databases

Genevestigatori P28322.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
InterProi IPR000418. Ets_dom.
IPR006715. ETS_PEA3_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF00178. Ets. 1 hit.
PF04621. ETS_PEA3_N. 1 hit.
[Graphical view ]
PRINTSi PR00454. ETSDOMAIN.
SMARTi SM00413. ETS. 1 hit.
[Graphical view ]
PROSITEi PS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of PEA3, a new member of the Ets oncogene family that is differentially expressed in mouse embryonic cells."
    Xin J.-H., Cowie A., Lachance P., Hassell J.A.
    Genes Dev. 6:481-496(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Lung.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.

Entry informationi

Entry nameiETV4_MOUSE
AccessioniPrimary (citable) accession number: P28322
Secondary accession number(s): Q3UMZ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: September 18, 2013
Last modified: July 9, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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